CN100415113C - Nuisance free special immunological growth promotor for bastard halibut and pleuronectid and its production process - Google Patents
Nuisance free special immunological growth promotor for bastard halibut and pleuronectid and its production process Download PDFInfo
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Abstract
The present invention discloses a formula of a special immunity growth promoter for nuisanceless fodder of bastard halibut and pleuronectid fishes and the preparation technology thereof. The effective component names and the proportional range thereof comprise: 28000 to 46000 mg/kg of oligomerization xylose, 21000 to 38000 mg/kg of bile acid, 15000 to 33000 mg/kg of bacillus, 13000 to 25000 mg/kg of lactic acid bacteria, 22000 to 35000 mg/kg of beta-dextran and 16000 to 25000 mg/kg of manna oligosaccharide, carriers are ordinary secondary powder, and the weight proportion range is from 10% to 50%. The preparation technology comprises: the weight addition level of monomeric additives is calculated according to effective component proportion, and the monomeric additives are uniformly mixed with the carriers below 30 DEG C in a workshop without direct sunlight projection according to a gradually enlarged mixed method; then the mixture is stirred for 5 to 6 minutes in a special stirrer made of stainless steel, and the variation coefficient CV of the mixing uniformity is below 5%. The special immunity growth promoter has the advantages of obvious actual cultivation test effect, stable water environment maintenance and high formula operability, and the special immunity growth promoter is a fire-new special immunity growth promoter with special effect for nuisanceless fodder of bastard halibut and pleuronectid fishes, and the fire-new special immunity growth promoter is suitable for national conditions. The special immunity growth promoter has broad application prospects in bastard halibut and pleuronectid fish cultivation and fodder production in China.
Description
Technical field
The present invention relates to animal feed nutrient formulation technology, short long agent prescription of specifically a kind of sea-farming left-eyed flounder nuisance free feed special immune and manufacture craft thereof.
Background technology
China has become the country that global unique aquaculture output surpasses fishery output, increased more than 6 times in the period of the sea-farming output 15, thereby the aquatic feeds demand significantly increases, and the ratio that accounts for domestic mixed feed total amount brings up to 12% by 2% before 15 years.Yet in sea-farming, ignore the aquatic animal characteristic, cause cultivating pool or marine environment eutrophication, disease, red tide etc. to be on the rise.The factor such as extensive except that breeding way, that facility is simple and crude, fresh food uses more, mixed feed popularity rate low (only having an appointment 30%), existing in addition compound feed products is simply rough, nutrient imbalance, drug abuse, residual bait are too much etc., makes the feed factor become the main root of water environment automatic pollution and product medicament residue.The aquatic livestock nutrition and the feed formula research that comprise the short long agent of immunity are bigger with developed country's gap, and the feed coefficient of China is mostly more than 2.0, and fishery developed country is below 1.0.Though it should be noted that in addition some new antibiotic substitutes additive and can promote growth or strengthen immunity, cause environmental pollution to increase the weight of simultaneously, at the cost of the environment promptly.The left-eyed flounder environment-protecting feed of nearly 2 years states such as Denmark has entered China, but its price is very expensive, and, make its feeding effect satisfied inadequately, and homemade left-eyed flounder environment-friendly feed product does not have development always because of culturing factors such as national conditions difference, transportation and storage period are long.Thereby China seawater left-eyed flounder is cultured and production of fodder demands having new and high technology content urgently, cost is lower, benefit is lasting and have the nuisance free feed and the core nutrition component thereof of independent intellectual property right.
The short long agent of immunity is as one of core microcomponent of modern mixed feed, in the fish body not as nutrient utilization, and bring into play functional effect with its unique texture, generally by the immune stimulatory cell, improve the alimentary canal micro-ecological environment, activate modes such as relevant physiological factor, strengthen the immune disease-resistance power of fish, strengthen the gastro-intestinal digestion enzyme activity, improving nutrient digests and assimilates and efficiency of feed utilization, thereby promotion fish growth, reduce the discharging of nitrogen phosphorus, the final purpose that realizes substituting antibiotic and minimizing or do not increase environmental pollution.Along with the increasing of antibiotic forbidding dynamics, the importance of nuisanceless class non-nutritive additive in feed is more outstanding in recent years.Cultivating condition is different with mode, the production purpose is different with level, formula material kind difference, feed processing technology are not equal, causes differences such as demand type to non-nutritive additive, assembled scheme, adding proportion.Do not have as yet at present at the short long agent prescription development of the immunity of China's deep-well seawater half industrial aquaculture left-eyed flounder mixed feed, the short long agent product of nuisance free feed special immune that adapts to the expanded pellet feed processing characteristic still belongs to blank at home.
Summary of the invention
For filling up above-mentioned blank, the purpose of this invention is to provide and a kind ofly be exclusively used in high-tech, low cost in the nuisanceless mixed feed of seawater left-eyed flounder, meet China and culture remarkable additive formulations and the manufacture craft that strengthens immunity and promote the growth effect of having of characteristics, it be inventor's one of core nutrition component of the swelling granular nuisance free feed of success development first at home.
To achieve these goals, technical solution of the present invention is: 1) grasping in recent years both at home and abroad green or nuisanceless class antibiotic substitute products progress and in mixed feed on the operating position basis, according to brand-new animal ecology nutrition principle, at utmost to reduce or not increase water pollution is target, close national conditions characteristic and modern aquatic products expanded pellet feed processing features in conjunction with China's deep-well seawater half industrial aquaculture, use modern nutrition and biotechnology new product, system and the short long agent prescription composition of the special-purpose special efficacy immunity of optimal design left-eyed flounder nuisance free feed synthetically.2) additive with development adds in definite nuisanceless mixed feed by 0.3-0.6% weight and (does not contain antibiotic), uses facility for granulating to be mixed with the half heavy dried pellet of property.3) carry out compbined test and mensuration such as growth of animal, immunity, digestion, aquatic attitude under the actual cultivating condition, prove the actual feeding effect and the nuisanceless effect of this mixed feed.4) according to test effect prescription is made amendment perfectly, finally determine prescription of the present invention and manufacture craft thereof.
The present invention's prescription specifically is made up of by definite part by weight green or nuisanceless additive and carrier more than 4 kinds, and its active ingredient title and proportion are: oligomeric xylose 28000-46000mg/kg, bile acid 21000-38000mg/kg, bacillus 15000-33000mg/kg, lactic acid bacteria 13000-25000mg/kg, beta glucan 22000-35000mg/kg, mannan-oligosaccharides 16000-25000mg/kg.Carrier is a normal diet with time powder (after adopting wheat to extract smart powder, remove wheat bran through mill, sieve and obtain, its protein wt content is about 14%, and granularity is generally at the 400-600 micron), and the part by weight scope is 10-50%.Its manufacture craft is: the weight addition of calculating each monomeric additive according to the active ingredient proportional meter, below 30 ℃, in the workshop of no sunlight direct projection, accurate every kind of raw material monomer of weighing, according to enlarging mixed method step by step, mix with carrier, in the special stainless steel agitator, stirred 5-6 minute then, the coefficient of variation CV that makes its mixture homogeneity is below 5%, particle diameter divides in the goldleaf bag of packing in the 400-600 micrometer range at last, and sealing is kept in Dark Place standby.
The present invention adopts the animal ecology nutrition principle of above-mentioned prescription as follows:
It is a new life's overlapping edges subject that ecoalimental is learned, it is to be based upon on the animal nutrition theoretical foundation, the principle of utilization ecology and systematics, by measures such as modern biotechnology and products thereof, processing technology, the schemes of feeding, animal and environment are carried out (comprising environment in the alimentary canal) science of nutrition regulation.
The balance that the animal ecology nutrition is particularly suitable for studying aquatic livestock nutrition and environment is a scientific basic of developing nuisanceless or environment-protecting feed with harmonious.Its main feature is: (1) can overcome the over-borrowing of traditional fishes and shrimps nutrient research has the very limitation of big-difference in view of the result data under the beaker of regulating and control or small-sized tank raising result and the aquatic attitude condition of actual breed; (2) the feeding quality measurement index is to reduce water pollution and grow of equal importance fast; (3) the throw something and feed foundation of management is needs of ingesting that are subjected to factor affecting such as water temperature, dissolved oxygen, rather than the artificial definite throwing amount of raising; (4) take into account the animal microecological balance simultaneously, promptly fall and metabolite by normal microflora, environment in the conditioning alimentary canal improves nutritional utilization and health status; (5) also consider to do effect, processing technology improvement etc. mutually between feed ingredient.Finally reach to significantly improve the animal feed nutrient utilization and promote and grow, alleviate environmental pollution to greatest extent, keep aquatic attitude balance, the control disease takes place, and produces low cost, high benefit, oligosaprobic nuisanceless environment-friendly feed.
The effect of active ingredient oligomeric xylose, bile acid, bacillus, lactic acid bacteria, beta glucan and mannan-oligosaccharides that the present invention adopts is respectively:
Xylo-oligosaccharide mainly comes from following approach to the growth promotion function of animal: 1. antagonistic action, promptly impel beneficial bacterium such as intestinal bifidobacteria to breed in a large number, pathogenic bacteria field planting such as competitive exclusion Escherichia coli, salmonella give birth to the site, thereby suppress pathogenic bacteria; 2. improve the animal non-specific immunity, strengthen body the pathogen resistance; 3. improve the intestinal microecology environment, suppress the growth of pathogenic bacteria, improve the ability of digesting and assimilating of feed; 4. decomposing harmful substances produces benefit materials such as organic acid, alleviates the ight soil stink, improves breeding environment.
Bile acid is in the digestion process of food, and bile acid not only plays auxiliary action of lipase, can strengthen the activity of lipase simultaneously, has the raising animal immunizing power, reduces the function of bacterial endotoxin uptake in the animal body.It can also promote animal ingestion, improves feed efficiency, reduce feeding cost.If animal is taken in an amount of deoxidation bile acid, just can effectively decompose endotoxin, safeguard the health of animal body.Bile acid still is a kind of effective bactericide.In the large intestine of animal, bile acid can suppress Escherichia coli, the propagation of streptococcus and other harmful bacteria.
The bacteriocin (peptide material) that bacillus produces has remarkable inhibitory action to Escherichia coli and Salmonella.Bacillus subtilis and bacillus licheniformis have stronger protease, amylase and lipase active.The enzyme that also has complex carbohydrates in the degrading plant forage simultaneously, as enzymes such as pectin, glucan, celluloses, wherein a lot of enzymes are enzymes that mammal and bird can not be synthesized in vivo.Bacterial strain such as bacillus coagulans, lactobacillus can produce lactic acid, can improve the utilization of animal to calcium, phosphorus, iron, promotes the absorption of vitamin D.
The lactic acid bacteria animal has health care and therapeutic efficiency, mainly shows: 1. nutriment is provided, has short body growth effect.If lactic acid bacteria can normally be brought into play metabolic activity in vivo, just can directly provide available essential amino acid (as lysine and methionine etc.) and various vitamin (vitamin B complex and K, H etc.) for the host, also can improve the BA of mineral element, closely be reached for the host essential nutriment is provided, strengthen the Nutrition and Metabolism of animal, directly the effect of short its growth.2. improve micro-ecological environment, cleaning enteron aisle noxious material.3. regulate digestion immune system etc., strengthen digestion and immunity.
Beta glucan can promote the growth performance of animal, improves feed efficiency, strengthens the immunity of animal body.At immunology, can improve the phagocytic activity of macrophage, increase functions of immune system.Glucan can strengthen the bacteriolyze function of animal blood plasma endocomplement system, can also promote the differentiation of cytotoxic T cell, and promotes the thick liquid cell that is differentiated by the B cell to produce the function of selectivity antibody.
Manna oligosacchride has the effect of two aspects: 1. have certain immunogenicity, can stimulate immune response, and it can combine with certain toxin, virus and fungal cell's surface and as the adjuvant of these exogenous antigens, slow down the absorption of antigen, increase tiring of antigen, thereby strengthen the cell and the humoral immune reaction of animal body.2. manna oligosacchride can be regulated and control the animal gastrointestinal tract micro-ecological environment, promote the growth and the breeding of beneficial bacterium such as Bifidobacterium, Bacillus acidi lactici etc., make it in intestines and stomach, form little ecologic competition advantage, directly suppress the growth and breeding of intrinsic spoilage organisms in external source bacterium and the intestines, thereby brought into normal play the normal function of gut flora in barrier, nutrition and immunity.
The present invention has following innovation and advantage:
1. main innovation of the present invention is, use brand-new animal ecology nutrition principle, under actual cultivating condition, carry out compbined test and mensuration such as growth of animal, immunity, aquatic attitude, develop the short long agent of the special-purpose special efficacy immunity of the nuisance free feed that is fit to China's deep-well seawater half industrial aquaculture left-eyed flounder characteristic and adapts to modern aquatic products expanded pellet feed processing characteristic first, fill up the blank of this type of feed product of China.
2. the both alternative antibiotic of long agent is urged in the present invention's immunity, making not, the nuisanceless mixed feed of added with antibiotic reaches immune disease-resistance and remarkable growth promotion usefulness, reach largely from systematology and ecological angle again and promote fish growth and aquatic attitude, harmony between nuisanceless, thereby both realized the real low cost (optimize assembly and be added to the basis in right amount) of product, obtained to culture the highest and sustainability of overall benefit again.Be made into dried pellet with this additive by the interpolation of 0.3-0.6% weight, under China's physical condition, carry out turbot growth, immunity and the test of aquatic attitude and test proof: every index is significantly increased (p＜0.05 or p＜0.01) than control group, and wherein haemocyte engulfs that vigor improves 67.2%, complement C3, C4 improve 18.8% and 38.1% respectively, rate of body weight gain improves 15.4%, protein efficiency ratio, PER improves 7.5%, feed coefficient reduces by 6.9%; And breeding water body chemical oxygen consumption (COC) (COD) and ammonia-nitrogen content etc. are not remarkable with control group difference; The feed cost of per gain weight reduces by 5.5%.
The technology of the present invention advanced person, workable, the market demand is big.The present invention obtains through experimental study, and is high in technological content, easy to operate in conjunction with closely actual, belongs to being fit to the short long agent of the special-purpose special efficacy immunity of brand-new left-eyed flounder nuisance free feed that national conditions are cultured.The left-eyed flounder that is suitable for cold water and cold warm water is the topmost marine fish of northern China, and it is southern coastal to be generalized to Fujian, Guangdong, Jiangsu etc. by " relay breed ".Add up and prediction according to definite, China's left-eyed flounder year cultured output is about 50,000 tons at present, will increase to more than 80,000 tons by 2010, needs nuisanceless mixed feed more than 120,000 tons in year, needs special-purpose special efficacy immunity to urge about 300 tons of long agent in year, and annual value of production is about more than 2,800 ten thousand yuan.
In a word, the technology of the present invention advanced person, actual culture experiment enhancing immunity and growth promotion effect are remarkable, keep water environment stable, increase economic efficiency, it is workable to fill a prescription, and is the short long agent of the special-purpose special efficacy immunity of brand-new left-eyed flounder nuisance free feed that is fit to the national conditions breed, and application prospect is very wide in China's left-eyed flounder breed and production of fodder.
The specific embodiment
Embodiment 1
Prescription is: Kang Weisu (containing 35% weight oligomeric xylose) 0.80kg, but Li Kang (containing 15% weight bile acid) 1.40kg, bacillus licheniformis (active ingredient weight content 35%) 0.43kg, lactobacillus lactis (active ingredient weight content 45%) 0.29kg, beta glucan (active ingredient weight content 35%) 1.10kg, manna oligosacchride (active ingredient weight content 15%) 1.07kg, common powder 4.91kg.By the manufacture craft operation, obtain the short long agent product 10kg of a kind of left-eyed flounder nuisance free feed special immune.
Add this formulation product by 0.6% weight in mixed feed, the content of oligomeric xylose, bile acid, bacillus, lactic acid bacteria, beta glucan, mannan-oligosaccharides is respectively (mg/kg) in the mixed feed: 168,126,90,78,132 and 96.
Its manufacture craft is: about 25 ℃, in the workshop of no sunlight direct projection, accurate every kind of raw material monomer of weighing, according to enlarging mixed method step by step, mix with carrier, stirred 5-6 minute in the special stainless steel agitator then, the coefficient of variation CV that makes its mixture homogeneity is below 5%, and particle diameter is in the 400-600 micrometer range, divide at last in the goldleaf bag of packing into, sealing is kept in Dark Place standby.
Embodiment 2
Difference from Example 1 is: add following formulation product by 0.5% weight in mixed feed, the content of oligomeric xylose, bile acid, bacillus, lactic acid bacteria, beta glucan, mannan-oligosaccharides is respectively (mg/kg) in the mixed feed: 175,140,115,85,135 and 100.
Prescription is: Kang Weisu (containing 35% weight oligomeric xylose) 1.00kg, but Li Kang (containing 15% weight bile acid) 1.87kg, bacillus licheniformis (active ingredient weight content 35%) 0.66kg, lactobacillus lactis (active ingredient weight content 45%) 0.38kg, beta glucan (active ingredient weight content 35%) 1.35kg, manna oligosacchride (active ingredient weight content 15%) 1.33kg, common powder 3.41kg.By the manufacture craft operation, obtain the short long agent product 10kg of a kind of left-eyed flounder nuisance free feed special immune.
Embodiment 3
Difference from Example 1 is: add this formulation product by 0.4% weight in mixed feed, the content of oligomeric xylose, bile acid, bacillus, lactic acid bacteria, beta glucan, mannan-oligosaccharides is respectively (mg/kg) in the mixed feed: 184,152,132,100,140 and 100.
Prescription is: Kang Weisu (containing 35% weight oligomeric xylose) 1.31kg, but Li Kang (containing 15% weight bile acid) 2.53kg, bacillus licheniformis (active ingredient weight content 35%) 0.94kg, lactobacillus lactis (active ingredient weight content 45%) 0.56kg, beta glucan (active ingredient weight content 35%) 1.75kg, manna oligosacchride (active ingredient weight content 15%) 1.67kg, common powder 1.24kg.By the manufacture craft operation, obtain the short long agent product 10kg of a kind of left-eyed flounder nuisance free feed special immune.
Application Example
The left-eyed flounder nuisance free feed special microelement additive of the present invention turbot test examples of feeding.
1. materials and methods
1.1 test with fish and grouping
Test is chosen one aquatic farm from Qingdao with turbot, and the average initial body weight 151.3 ± 15.2g of juvenile fish is sterile-processed, behind the raising domestication 20d, selects physical health, individual juvenile fish 66 tails of uniform size as the test fish.The design of employing randomized block experiment, the examination fish is assigned in 2 groups, and every group 33 tail fish established 3 repetitions.2 groups of initial average weights of test fish are through the variance test of homogeneity, and group difference is remarkable (p＞0.05) not, begins formal test then.
1.2 test feed
Table 1 experimental basis daily ration prescription is formed and the main nutrient composition analysis
Annotate: NFE is a calculated value, and all the other are measured value
The basal diet of each processed group is identical, and its prescription determines that through further optimizing primary raw material is high-quality fish meal, fish oil, starch, composite mineral matter and B B-complex, sees Table 1 on the early-stage Study basis.Do not add or add the short long agent of special immune of the present invention (embodiment 1 formulation product) and form the different tests processed group in basal diet: control group: the basal diet of feeding, feed price are 8 yuan/kg; Test group: the short long agent (embodiment 1 formulation product) of basal diet+0.6% special immune, feed price is 8.12 yuan/kg.Test feed is mixed with the half heavy dried pellet of property that diameter is 8mm through pelleter.
1.3 growth test method
Animal experiment carried out in Chinese Qingdao April to July, and the formal test phase is 72d altogether, early stage 38d, later stage 34d.Adopt and culture the breed of drum circulating seawer.Each drum is equipped with a gas stone.Enter after the system handles such as the sand filtration of circulating seawer process, ozone and culture bucket, its flow is 0.8L/min.Illumination every day is 14h light: 10h is dark.The duration of test water quality condition is: temperature 12-20 ℃, and salinity 30-31, pH7.8-8.4, dissolved oxygen content 6-8mg/L.
Every day, throwing was raised 2 times, and throwing the amount of raising every day is 2～2.5% of fish body weight, and the process of at every turn throwing something and feeding continues 30min to 1h, drew remaining bait afterwards and deducted from the throwing every day amount of raising after 65 ℃ of oven dry constant weights.According to each the group fish changes of weight and the situation of ingesting, in time adjust feeding volume.Carrying out empty stomach fish body respectively when on-test and end weighs.
Examination sashimi (raw fish) long pointer is calculated as follows: rate of body weight gain (WGR) %=100 * (end weight-initial weight)/initial weight; Specific growth rate (SGR) %d-1=100 * (Ln end weight-Ln initial weight)/breed fate; Feed coefficient (FCR)=food ration/(end weight-initial weight); Protein efficiency ratio, PER (PER)=(end weight-initial weight)/(food ration * dietary protein level); The feed cost of per gain weight (CWG)=feed coefficient (FCR) * granular mixed feed price.
1.4 immune indexes assay method
Finish the same day at growth test, health examination fish 3 tails of the medium body weight of every test group picked at random carry out immune indexes and measure.From the fish tail venous blood collection, be divided into two parts with asepsis injector.After a room temperature leaves standstill 1h, 4 ℃ of refrigerations 12h that spends the night, the centrifugal 15min of 4000r/min next day draws supernatant, is used for SOD, lysozyme, complement C3 and C4 vitality test.Another part injects centrifuge tube in blood and 1: 1 ratio of anticoagulant heparin agent, carries out haemocyte and engulfs vitality test.Wherein the SOD vigor with Nanjing build up bio-engineering research testing cassete measure, complement C3, C4 vigor are measured with the testing cassete of health Bioisystech Co., Ltd of Erie, antalzyme activity uses the Hultmark method to measure, and haemocyte is engulfed vigor and used U.S. FACSVantage SE cells were tested by flow cytometry.
1.6 water quality factor assay method
Water quality factor is determined at the 60th day of growth test to carry out.Measuring fed morning on the same day finish after, the remaining bait of sucking-off is all changed breeding seawater, closes recirculated water, normal ventilation then.After examination steck excrement peak period (the about 8-10h in back feeds), evenly gather water sample 300ml for every barrel, insert-20 ℃ of freezing preservations, use the full-automatic water quality analyzer of Dutch Skalar sanplus, the concentration of ammonia nitrogen (NH3-N), nitre nitrogen (NO3-N), nitrite nitrogen (NO2-N) and phosphate (PO4) in the test sample in the 24h.COD measures (National Bureau of Oceanography, 1991) by the national standard potassium permanganate oxidation method.
1.5 statistical analysis
All indicator-specific statistics analyses adopt the variance analysis method of RANDOMIZED BLOCK DESIGN in the SPSS10.0 statistical software to carry out, and multiple ratio adopts LSD, DuncanShi method to carry out.
2. result of the test
2.1 test on immune function result
By table 2 as seen, test group fish blood complement C3 and C4 vigor, SOD vigor, antalzyme activity and cytophagy vigor all have significantly than control group or the utmost point significantly improves, and be more remarkable to the effect of complement C3 and cytophagy vigor.The utmost point significantly improves 18.8% and 67.2% (p＜0.01) according to organizing respectively for test group complement C3 vigor and the comparison of cytophagy vigor, and complement C4 vigor, SOD vigor and antalzyme activity comparison significantly improve 38.1%, 13% and 2.1% (p＜0.05) respectively according to group.
The short long agent of table 2 immunity is to the influence of young turbot haematogenic immunity factor vigor
Annotate: 1) numerical value is mean+SD in the table, the identical expression group difference of same column data shoulder motes letter is remarkable (P＞0.05) not, the adjacent expression group difference of shoulder motes letter is (P＜0.05) extremely significantly, and shoulder motes letter spacing is represented group difference extremely significantly (P＜0.01), and is as follows.
2) engulfing vigor is the total cell with phagocytic activity and the ratio of total blood cell.
2.2 growth test result
As known from Table 3, test group has significantly improved the growth performance of turbot than control group, specific growth rate, efficiency of feed utilization and protein efficiency ratio, PER improve 12% (p＜0.05), 6.9% (p＜0.05) and 7.5% (p＜0.01) respectively, and the feed cost of per gain weight reduces by 5.5%.
The short long agent of table 3 immunity is to the influence of growth, feed conversion and the weightening finish cost of young turbot
2.3 water quality factor measurement result
Table 4 shows, compares with control group, and test group has certain influence to fish water environment factor variations of living in, but statistical analysis difference not significantly (p＞0.05).Ammonia nitrogen in the test group water body, nitrite nitrogen, phosphate and the comparison of COD content according to component you can well imagine high by 5.3%, 4.9%, 4.9% and 1.9%.
The short long agent of table 4 immunity is to the influence (ug/L/D/ bar, COD:g/L/D/ bar) of the growth turbot water environment factor
4. brief summary:
1, test is measured by the turbot nospecific immunity factor and growth performance and be the analysis showed that the test group effect is good, compares with control group, and complement C3 vigor improves 18.8% (p＜0.01), complement C4 vigor improves 38.1% (p＜0.05); The SOD vigor improves 13% (p＜0.05); Antalzyme activity improves 2.1% (p＞0.05); The cytophagy vigor improves 67.0% (p＜0.01); Specific growth rate improves 12% (p＜0.05); Feed coefficient decline 6.9% (p＜0.05); Three nitrogen and COD content improve 5.0% and 1.9% (p＞0.05) respectively in the water body.The feed cost of per gain weight reduces by 5.5%.
2, this experimental study presentation of results, after being made into the environmental protection granular mixed feed with the short long agent of special immune of the present invention, significantly strengthen the immunologic function and the growth performance of growth turbot, efficiency of feed utilization and economic benefit obviously improve, and less to the water quality factor variable effect.The nuisanceless environment-friendly feed that belongs to low-cost, low pollution, high benefit.
Claims (5)
1. short long agent of left-eyed flounder nuisance free feed special immune, it is characterized in that: count by weight, plain 0.80 part of the Kang Wei that contains 35% weight oligomeric xylose, what contain 15% weight bile acid can sharp health 1.40 parts, the active ingredient weight content is 0.43 part of 35% a bacillus licheniformis, the active ingredient weight content is 0.29 part of 45% a lactobacillus lactis, the active ingredient weight content is 1.10 parts of 35% beta glucans, the active ingredient weight content is 1.07 parts of 15% manna oligosacchrides, 4.91 parts in common powder.
2. short long agent of left-eyed flounder nuisance free feed special immune, it is characterized in that: count by weight, plain 1.00 parts of the Kang Wei that contains 35% weight oligomeric xylose, what contain 15% weight bile acid can sharp health 1.87 parts, the active ingredient weight content is 0.66 part of 35% a bacillus licheniformis, the active ingredient weight content is 0.38 part of 45% a lactobacillus lactis, the active ingredient weight content is 1.35 parts of 35% beta glucans, the active ingredient weight content is 1.33 parts of 15% manna oligosacchrides, 3.41 parts in common powder.
3. short long agent of left-eyed flounder nuisance free feed special immune, it is characterized in that: count by weight, plain 1.31 parts of the Kang Wei that contains 35% weight oligomeric xylose, what contain 15% weight bile acid can sharp health 2.53 parts, the active ingredient weight content is 0.94 part of 35% a bacillus licheniformis, the active ingredient weight content is 0.56 part of 45% a lactobacillus lactis, the active ingredient weight content is 1.75 parts of 35% beta glucans, the active ingredient weight content is 1.67 parts of 15% manna oligosacchrides, 1.24 parts in common powder.
4. according to claim 1 or the short long agent of 2 or 3 described left-eyed flounder nuisance free feed special immunes, it is characterized in that: the interpolation part by weight in perfect compound feed is 0.3-0.6%.
5. urge the manufacture craft of long agent according to claim 1 or 2 or 3 described left-eyed flounder nuisance free feed special immunes, it is characterized in that: the weight addition of calculating each monomeric additive according to the active ingredient proportional meter, below 30 ℃, in the workshop of no sunlight direct projection, accurate every kind of raw material monomer of weighing, according to enlarging mixed method step by step, mix with carrier, in the special stainless steel agitator, stirred 5-6 minute then, the coefficient of variation CV that makes its mixture homogeneity is below 5%, divide at last in the goldleaf bag of packing into, sealing is kept in Dark Place standby.
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| CN101095465B (en) * | 2007-07-12 | 2011-04-06 | 章爱云 | Application of low-polyxylose complicated preparation in the preparing of feedstuff that is capable of enhancing immunity of marine animals |
| CN102450514B (en) * | 2010-12-06 | 2013-06-26 | 山东新希望六和集团有限公司 | Compound crucian feed and preparation method thereof |
| CN103844145A (en) * | 2014-03-12 | 2014-06-11 | 江苏天成科技集团有限公司 | Method for producing novel loach feed |
| CN104171334A (en) * | 2014-08-08 | 2014-12-03 | 江苏华威农牧发展有限公司 | Feed capable of preventing fading of spots of channel catfish |
| CN114617203A (en) * | 2020-12-14 | 2022-06-14 | 江苏大北农水产科技有限公司 | Functional feed for aquatic animals and using method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1463615A (en) * | 2002-06-14 | 2003-12-31 | 湖北畅响生物工程股份有限公司 | Microbiologically active prawn fodder and process for producing same |
| US20040161422A1 (en) * | 1999-04-30 | 2004-08-19 | Natarajan Ranganathan | Nutritional compositions comprising probiotics |
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| US20040161422A1 (en) * | 1999-04-30 | 2004-08-19 | Natarajan Ranganathan | Nutritional compositions comprising probiotics |
| CN1463615A (en) * | 2002-06-14 | 2003-12-31 | 湖北畅响生物工程股份有限公司 | Microbiologically active prawn fodder and process for producing same |
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