CN106721053A - A kind of utilization composite bacteria is to yeast wastewater and the method for maize alcohol lees hybrid solid fermenting and producing biological protein feedstuff - Google Patents
A kind of utilization composite bacteria is to yeast wastewater and the method for maize alcohol lees hybrid solid fermenting and producing biological protein feedstuff Download PDFInfo
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Abstract
The invention discloses a kind of utilization composite bacteria to yeast wastewater and the method for maize alcohol lees hybrid solid fermenting and producing biological protein feedstuff.Comprise the following steps：With maize alcohol lees as raw material, yeast wastewater is auxiliary material；Maize alcohol lees and yeast wastewater are mixed；According to quality of material after mixing 0.2% 0.5% add composite bacteria to be fermented, and fermentation temperature is 20 DEG C 55 DEG C, and fermentation time is 40 72 hours；Material after fermentation be dry, pulverize into packaging in 50 75 DEG C of fluid beds, biological protein feedstuff is obtained.The present invention is fermented using yeast wastewater and maize alcohol lees first, belongs to domestic initiation.The inventive method process is simple, operation is easy, with short production cycle, low production cost, good product quality.The present invention turns waste into wealth yeast production byproduct fermentation concentrated water, and the sugar source for solving China wastes situation, while being also beneficial to promote the environmental protection cause of China.
Description
Technical field
Present invention relates particularly to one kind using composite bacteria to yeast wastewater and maize alcohol lees hybrid solid fermenting and producing
The method of biological protein feedstuff.
Background technology
Yeast production is main with molasses as raw material, wherein based on cane molasses and beet molasses, what yeast production was produced
Clear liquid is mainly derived from molasses in itself.Molasses are the accessory substances after the sugaring of sugar refinery, wherein containing abundant sugar, in molasses can
After being utilized by yeast fermentation using sugar, the metabolite that the material and yeast metabolism that residue is not utilized by yeast are produced is most
Can be discharged with fermentation separating liquid eventually.Therefore, it is single containing inorganic microelements such as abundant nitrogen, phosphorus, potassium, calcium, iron in yeast wastewater
The larger molecular organicses such as sugar, Yeast protein, abundant protease and caramel, humic acid, fulvic acid.
Chen Peijin etc. discloses one kind molasses as main material production yeast, is followed using primary wastewater and secondary waste water
Ring is utilized, and stream is added in yeast fermentation tank, used as thinned water (Chen Peijin, Qiu Jihuai, Wu Nan the monarch's ferment for being continuously fermented
Research [J] the Environmental Pollution and Controls of female cycling utilization of wastewater, 1993 (5):12-14.).In the technology of this article, using ferment
The mode that mother stock adds from waste water direct reuse stream.Due to present yeast production requirement high gravity fermentation, the yeast of vigor high is produced,
This mode is difficult composite high concentration fermentation and requires, while obtained yeast activity will not be very high.
Yeast wastewater with molasses as raw material belongs to high concentrated organic wastewater, and its biochemical process performance is poor, single
Treatment technology yeast wastewater can not be processed after qualified discharge.Due in yeast fermentation waste water containing abundant organic matter and
Various trace elements.Therefore, the treatment for yeast wastewater must be on the basis of comprehensive utilization of resources be realized using various
Treatment technology is combined, and finally realizes the qualified discharge of waste water.How to realize that turning waste into wealth for yeast wastewater is with great meaning
The problem of justice.
Maize alcohol lees are the trade names of vinasse albumen feed, i.e. the distiller's dried grain containing soluble solids.It is being with corn
During fermenting raw materials ethanol production, starch therein is converted to ethanol and carbon dioxide, other nutritional ingredients such as protein,
Fat, fiber etc. are stayed in vinasse.Simultaneously because the effect of microorganism, protein, B family vitamin and amino acid contain in vinasse
Amount increased than corn, and contain the unknown somatomedin generated in fermentation.
But the feed prepared using maize alcohol lees, is had the disadvantage that：
(1) alcohol grain crude fiber content is (up to 10%) higher, makes the protein and other organic matter digestibilities of alcohol grain feed
Reduce.
(2) according to this toward document report, the poorly soluble of maize alcohol lees protein, this causes it in the digestion of monogastric
Rate reduction.
(3) yeast thalline therein (wu etc., 1981) is come from containing 9% protein in maize alcohol lees.These thalline
Cell membrane in the digested difficulty of domestic animal intestines and stomach.This aspect makes cell membrane nutrient contained in itself nondigestible, the opposing party
Also nutritional ingredient is digested and assimilated in block cell in face.
(4) due to organic acid of the alcohol grain containing some fermentation generations.So, the larger (pH value one of former grain liquid of its acidity
As 4 or so), this can accelerate gastrointestinal peristalsis, so as to shorten residence time of the chyme in alimentary canal, drop nutrient digestibility
It is low.
The content of the invention
It is an object of the invention to make full use of yeast wastewater to produce protein feed raw material, yeast is on the one hand processed and has given up
Water, reduces environmentally friendly cost, and still further aspect can produce certain economic benefit for yeast wastewater.
The technical solution used in the present invention is：
It is a kind of to yeast wastewater and the method for maize alcohol lees hybrid solid fermenting and producing biological protein feedstuff, including following step
Suddenly：
1) with maize alcohol lees as raw material, yeast wastewater is auxiliary material；Maize alcohol lees and yeast wastewater are mixed, mixture
The mass percent of yeast wastewater is 25%-45%, balance of maize alcohol lees in material；
2) composite bacteria is added according to the 0.2%-0.5% of quality of material after mixing, wherein composite bacteria is aspergillus oryzae, saccharomycete
With the mixed bacteria of lactic acid bacteria, aspergillus oryzae, saccharomycete and lactic acid bacteria mass ratio are (1.5-2.5)：(1.5-2.5)：(0.8-
1.2)；
3) mixed material is fermented, fermentation temperature is 20 DEG C -55 DEG C, fermentation time is 40-72 hours；By the thing after fermentation
Expect to be dried to moisture in 50-75 DEG C of fluid bed and be not higher than 12%, then crushing packing.
Preferably, in selected maize alcohol lees, alcohol Egg preserved in wine is controlled between 25%-40% in vain, and moisture is controlled in 10%-
15%, by mass percentage.
Preferably, the content control of yeast wastewater solid matter is in 45%-50%, albumen quality control 15%-20%, nothing
Machine nitrogen is controlled within 1.5%, by mass percentage.If not by protein in yeast wastewater between 15% to 20%,
To be substantially reduced as efficiency of feed utilization.
Preferably, step 1) in yeast wastewater and maize alcohol lees by water content detection, carry out dispensing by above-mentioned formula, obtain
To mixed material, material moisture is 35%-45% after controlling mixing.
Preferably, aspergillus oryzae, saccharomycete and lactic acid bacteria mass ratio are (1.8-2.2) in composite bacteria：(1.8-2.2)：1.
Aforementioned proportion is optimal proportion, and wherein saccharomycete is protein Candida, if using other strains such as saccharomyces cerevisiae
Deng reducing sugar will be improved, so as to influence absorbable material protein content.
Preferably, the mass percent of yeast wastewater is 30%-40%, balance of maize alcohol lees in mixed material.
Preferably, aspergillus oryzae viable count is not less than 5,000,000,000/g in composite bacteria, and yeast viable count is not less than 20,000,000,000/g, breast
Sour bacterium viable count is not less than 10,000,000,000/g.
Preferably, mixing is added after composite bacteria is recovered first with mass percent for the G/W of 1%-2%
In material.
Preferably, at 30-45 DEG C, fermentation optimum time is 70-75 hours to fermentation temperature.
Preferably, the material moisture after drying controls to cross 40-80 mesh after 10%-12%, crushing.
The beneficial effects of the invention are as follows：
The present invention is fermented using yeast wastewater and maize alcohol lees first, belongs to domestic initiation.The present invention utilizes Mixed Microbes
The method that kind fermentation process produces modified peptide protein to yeast wastewater and maize alcohol lees mixed fermentation, the method process is simple,
Operation is easy, with short production cycle, low production cost, good product quality.
The present invention is to ferment concentrated water as auxiliary material using yeast, is bacterial strain using the abundant monose and inorganic nitrogen in concentrated water
Energy and nitrogen source that fermentation is provided, are conducive to strain growth to be metabolized, the coarse-fibred content of effective degrading maize alcohol grain, pass through
The self-reproduction of yeast military sports, effectively improves the content of raw material absorbable proteins.Organic protein after the fermentation that the present invention is obtained
Absorbability albumen is significantly improved in raw material, containing a large amount of surfactant polypeptides, small peptide and monose etc., also abundant yeast egg
In vain, bioavailability is high, is conducive to digesting and assimilating for aquatic livestock.
Composite bacteria and its proportioning that fermentation process of the present invention is used, the beneficial bacteria that animal body intestinal microecology can be promoted to balance
Group, comprehensive producing enzyme acid producing ability is strong：The substantial amounts of high molecular weight protein of albumen in maize alcohol lees can be not only degraded into solvable
Property small protein, polypeptide and free amino acid；Also cellulose decomposition into monose and oligosaccharides can be greatly improved raw material utilization
Rate.The nutrition of fermentation product is balanced, product appearance yellowish-brown, special fragrance and alcohol fragrance with fermented product.
Product of the present invention is by after fluid bed low temperature drying, probiotics can keep activity, the micro- life of enteron aisle to animal substantially
State balance has certain facilitation, can promote amylase and proteinase activity in enteron aisle, is conducive to the growth of animal and disappears
Change performance, strengthen animal body immunity.
The present invention turns waste into wealth yeast production byproduct fermentation concentrated water, and the sugar source for solving China wastes situation, while
It is also beneficial to promote the environmental protection cause of China.
Specific embodiment
The waste water that terminology used in the present invention " yeast wastewater " is produced during referring to production yeast, including after yeast separation
Supernatant and yeast slurry, also referred to as yeast clear liquid contains the materials such as melanin higher, phenols and caramel, face
Color is deeper, in brownish black；Containing about 0.5% dry in yeast wastewater, main component is yeast protein, cellulose, colloid
Nutritional ingredient such as residual sugar in material and the blackstrap not being fully utilized etc..
Maize alcohol lees selected by the present invention be extract corn oil after corn germ protein, protein content 25% with
On, fat content is below 3%.Auxiliary material selected by the present invention is yeast wastewater, is the waste water after our company's production yeast, is entered
Row is concentrated by evaporation, and controls the content of solid matter in waste water for 45%-50%, can improve the amount of solid content in waste water, increases
Given money as a gift in raw material the loading of waste water, between wherein albumen quality control 15%-20%, inorganic nitrogen is controlled within 1.5%.
With reference to specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
(1) supplementary material mixing and inoculation
Prepare raw material maize alcohol lees 1200kg, its water content is 12%, and the protein content of maize alcohol lees is controlled 30%；It is auxiliary
Expect to be to isolate the fermentation waste water after yeast in yeast wastewater, that is, yeast production, weight is 800kg, wherein yeast wastewater
The content of solid matter is 45%, and 18%, inorganic nitrogen is controlled 1.2% albumen quality.By maize alcohol lees and yeast wastewater
It is sufficiently mixed, material moisture is 40%-45% after regulation mixing, material mixture homogeneity is less than 10%；
Weigh 4 kilograms of aspergillus oryzaes (viable count is not less than 5,000,000,000/g), 4 kilograms of candida utili (viable count is not less than 20,000,000,000/
G) with 2 kilograms of lactic acid bacterias (viable count is not less than 10,000,000,000/g)；Then activation culture half is carried out using the G/W of 1%-2%
Hour, it is uniformly added into mixed material using current add mode.
(2) ferment
Using box ventilating fermentation mode, steam source uses hot-blast stove, and yeasting temperature control is in 20-45 degree, moisture control
35%-40% processed, ferments 72 hours.When temperature drops to 50 degree or so, into drying procedure.
(3) dry
After fermentation ends by material in 50-75 DEG C of fluid bed dry to moisture 10% or so material, dry after material pass through
Packed after 40 mesh sieves excessively after beater disintegrating machine crushing and got product.
Crude protein 33%-36%, sour molten albumen 1%-3%, sulphur glucoside 50mmol/g before fermentation, probiotics 0.1 hundred million/gram.Hair
Finished product inner quality standard result is as follows after ferment, is shown in Table 1.
Table 1
As shown in Table 1：Absorbability albumen is significantly improved in organic protein raw material after the fermentation that the present invention is obtained, containing a large amount of activity
Substance polypeptide, small peptide and monose etc., also abundant Yeast protein, bioavailability are high, are conducive to the digestion of aquatic livestock to inhale
Receive.
Embodiment 2
(1) supplementary material mixing and inoculation
Prepare raw material maize alcohol lees 1400kg, its water content is 10%, and alcohol Egg preserved in wine is controlled 40% in vain；Auxiliary material is that yeast is dense
Water, weight is 600kg, and wherein the content of yeast wastewater solid matter is 50%, and albumen quality is 20%, and inorganic nitrogen control exists
1.3%.Maize alcohol lees are sufficiently mixed with yeast wastewater, material moisture is 35%-40%, material after regulation mixing
Mixture homogeneity is less than 10%；
Weigh 3.6 kilograms of aspergillus oryzaes (viable count is not less than 5,000,000,000/g), (viable count is not less than 200 to 3.6 kilograms of candida utili
Hundred million/g) and 2.0 kilograms of lactic acid bacterias (viable count is not less than 10,000,000,000/g)；Then activation training is carried out using the G/W of 1%-2%
Half an hour is supported, is uniformly added into mixed material using current add mode.
(2) ferment
Using box ventilating fermentation mode, using box fermentation, heap is high 1 meter, and 1.5-2 tons of material, steam source are filled per fermenting case
Using hot-blast stove, fermentation temperature 30-45 degree, raw material moisture is controlled in 35%-45%, fermentation time 70 hours.When temperature is dropped to
When 50 degree or so, into drying procedure.
(3) dry
The material of moisture 12% or so will be dried in material 50-75 DEG C of fluid bed of grain after fermentation ends, material passes through after drying
Packed after 60 mesh sieves excessively after beater disintegrating machine crushing and got product.
Embodiment 3
(1) supplementary material mixing and inoculation
Prepare raw material maize alcohol lees 1300kg, its water content is 15%, and albumen is controlled 25%, and auxiliary material is yeast wastewater,
It is exactly to isolate the fermentation waste water after yeast in yeast production, weight is 700kg, the content of solid matter wherein in yeast wastewater
It is 45%, 15%, inorganic nitrogen is controlled 1.2% albumen quality.Maize alcohol lees are sufficiently mixed with yeast wastewater, are adjusted
Material moisture is 35%-40% after section mixing, and material mixture homogeneity is less than 10%；
Weigh 3.8 kilograms of aspergillus oryzaes (viable count is not less than 5,000,000,000/g), (viable count is not less than 200 to 4.2 kilograms of candida utili
Hundred million/g) and 2 kilograms of lactic acid bacterias (viable count is not less than 10,000,000,000/g)；Then activation culture is carried out using the G/W of 1%-2%
Half an hour, it is uniformly added into mixed material using current add mode.
(2) ferment
Using box ventilating fermentation mode, steam source uses hot-blast stove, and yeasting temperature control is in 20-45 degree, moisture control
35%-40% processed, ferments 70-75 hours.When temperature drops to 50 degree or so, into drying procedure.
(3) dry
After fermentation ends by material in 50-75 DEG C of fluid bed dry to moisture 12% or so material, dry after material pass through
Packed after 60 mesh sieves excessively after beater disintegrating machine crushing and got product.
Culture experiment checking is carried out using biological protein feedstuff of the invention.
Culture experiment one
1) test period and place
Test is carried out in Xiaogan in April, 2016~October with star animal husbandry.
2) materials and methods
Sanhuang chicken is 28 ages in days stalwartness chicken.Broiler chicken is randomly divided into 4 treatment, each 4 repetition for the treatment of, and each repeats 30 plumages.
Wherein, 1 is processed：Feeding complete feed of the autogamy without any antibiotic, treatment 2：10% embodiment 1 is added in daily grain of chicken to prepare
The biological protein feedstuff for obtaining, treatment 3：The biological protein feedstuff that 20% embodiment 1 is prepared is added in daily grain of chicken, is processed
4：The biological protein feedstuff that 30% embodiment 1 is prepared is added in daily grain of chicken.
3) feeding and management
Feeding manner is raised scattered for ground, free choice feeding, drinking-water.
4) project is determined
Performance test：In 56,91,120 ages in days randomly selected from each treatment 20 plumage broiler chicken weighed, ramming material, calculate
The average daily feed consumption rate of broiler chicken, Average weight increasing a day and feedstuff-meat ratio.Appetite, the healthy and death condition of each treatment chicken of observation.
5) experimental result and analysis
The results are shown in Table 2.
Table 2：Influence of the biological protein feedstuff to meat chicken production performance is added in daily ration
According to the data of table 1, by whole experiment it can be calculated that treatment 2,3,4 is compared with 1 (control) for the treatment of, Average weight increasing a day
7.02%, 6.95% and 11.18% is respectively increased, feed intake has been respectively increased 5.95%, 5.82% and 11.11%.In material
Meat compares aspect, and treatment 2,3,4 reduces by 1%, 1%, 1% than control；In terms of the incidence of disease, treatment 2,3,4 is reduced respectively than control
12%, 6% and 6%.Comprehensive indices understand, biological protein feedstuff is added in daily grain of chicken, can reduce aquaculture cost,
Improve survival rate and economic benefit.
In sum, the present invention adds 10%, 20% or 30% biological protein feedstuff of the invention in daily ration of broiler, has
Beneficial to the Average weight increasing a day, the feed intake that improve broiler chicken, the death rate, the incidence of disease, feedstuff-meat ratio are reduced, aquaculture cost can be reduced, improved
Survival rate and economic benefit.
Raise the three of feeding grain scattered in the Sanhuang chicken cultivated with normal diet to the Sanhuang chicken that treatment 2-4 is obtained, and rural area
Yellow chicken carries out meat, eggs and milk quality contrast experiment.
Result shows：Sanhuang chicken (treatment 2-4) meat, eggs and milk quality of biological protein feedstuff feeding of the present invention is significantly improved, excellent
In the Sanhuang chicken that normal diet is fed.It is embodied in：Chicken color is deeper, yellowly, raises the three of feeding grain scattered with rural area
Yellow chicken quality is suitable.Meanwhile, Sanhuang chicken of the invention produces egg, and the quality and color of eggshell are improved, and egg white thickness is thick, egg
It is yellow scarlet.After biological protein feedstuff 60 days；Average egg weight increases 1--3 grams, egg-laying peak growth；Laying period, is long
Phase uses, it is minimum increase laying period 1--2 month, 4--5 jins of egg of average every chicken fecund, additionally, Sanhuang chicken be difficult it is sick superseded,
And feather light increases the price sold.
Culture experiment two
1) test period and place
Test and assisting large group pig farm in 2 months 2016.
2) materials and methods
To 3000 sow breeding stocks, contrast effect：
From piglet 120, point control group and each 60 of experimental group；Pig 120 from, point control group and experimental group each 60
Head；From big porker 120, point control group and each 60 of experimental group.
Control group from dregs of beans (addition consumption be respectively 10%, 15%, 20%) as daily ration, experimental group with 10%,
15%th, the biological protein feedstuff of 20% embodiment 2 replaces complete diet pellet.
3) experimental result：
3.1) the piglet stage：
Experimental group：Pig end average weight be：21.52kg, average daily gain 467.7g, feed consumption rate is 19.79kg, and feed-weight ratio is
1.42：1；
Control group：Pig end average weight be：20.08kg, average daily gain 420.7g, feed consumption rate is 19.05kg, and feed-weight ratio is
1.51：1；
Experimental result data shows：In piglet stage food consumption reduction by 6%, average daily gain improves 47g to experimental group, improves
11.17%, diarrhea rate reduction by 6.6%, overall incidence reduction by 13.14%, feedstuff-meat ratio improves 0.09 again, reduces food consumption
6%.
3.2) the pig stage in：
Experimental group：Pig end average weight be：48.46kg, average daily gain：607g, feed consumption rate is 40.97kg, and feed-weight ratio is：
2.25∶1；
Control group：Pig end average weight be：47.10kg, average daily gain：525g, feed consumption rate is 39.85kg, and feed-weight ratio is：
2.53：1；
Experimental result data shows：In the middle pig stage, daily gain improves 15.62% to experimental group, and feed-weight ratio improves 0.28, reduces and raises
Expect that consumption is：11.11%.
3.3) the big porker stage：
Experimental group：Pig end average weight be：86.9kg, average daily gain 890g, feed consumption 72.2kg, feed-weight ratio is：2.7：1；
Control group：Pig end average weight be：86.7kg, average daily gain 840g, feed consumption 73.42kg, feed-weight ratio is：2.9：1；
Experimental data shows：Experimental group improves 50g in the average daily gain of big porker stage, improves 10%, and feed-weight ratio improves：0.2, drop
Low food consumption 2%.
Result finds：Compared with the pig of control group, the pig (including piglet, middle pig and big porker) of experimental group uses bioprotein
After feed 3~5 days, it is found that the appetite of pig is more vigorous；After biological protein feedstuff 7~10 days, the stool colour of pig is deepened, excrement
Just bulk, iblet is reduced or disappeared, the reduction of colony house ammonia concentration；After biological protein feedstuff 20~30 days, the skin of pig
Ruddy, bright in color；And good palatability, feed intake is significantly improved by comparing, using biological protein feedstuff of the invention, can
The cycle is delivered for sale to shorten pig 6-12 days.
So, using biological protein feedstuff of the invention, can reduce feedstuff-meat ratio, improve survival rate, strengthen immunity with
The cycle is delivered in premunition, shortening for sale.
Above-described embodiment is the present invention preferably implementation method, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from Spirit Essence of the invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (9)
1. a kind of to yeast wastewater and the method for maize alcohol lees hybrid solid fermenting and producing biological protein feedstuff, its feature exists
In：Comprise the following steps：
1）With maize alcohol lees as raw material, yeast wastewater is auxiliary material；Maize alcohol lees and yeast wastewater are mixed, mixture
The mass percent of yeast wastewater is 25%-45%, balance of maize alcohol lees in material；
2）According to after mixing quality of material 0.2%-0.5% add composite bacteria, wherein composite bacteria be aspergillus oryzae, saccharomycete and
The mixed bacteria of lactic acid bacteria, aspergillus oryzae, saccharomycete and lactic acid bacteria mass ratio are (1.5-2.5)：(1.5-2.5)：(0.8-1.2)；
3）Mixed material is fermented, fermentation temperature is 20 DEG C -55 DEG C, fermentation time is 40-72 hours；By the thing after fermentation
Expect to be dried to moisture in 50-75 DEG C of fluid bed and be not higher than 12%, then crushing packing.
2. method according to claim 1, it is characterised in that：In selected maize alcohol lees, control exists alcohol Egg preserved in wine in vain
25%-40%, moisture is controlled in 10%-15%, by mass percentage.
3. method according to claim 1, it is characterised in that：The content of yeast wastewater solid matter is controlled in 45%-50%,
Albumen quality controls 15%-20%, inorganic nitrogen to control within 1.5%, by mass percentage.
4. method according to claim 1, it is characterised in that：The mass percent of yeast wastewater is 30%- in mixed material
40%, balance of maize alcohol lees.
5. method according to claim 1, it is characterised in that：Aspergillus oryzae, saccharomycete and lactic acid bacteria quality in composite bacteria
Than being (1.8-2.2)：(1.8-2.2)：1.
6. method according to claim 1 or 5, it is characterised in that：In composite bacteria aspergillus oryzae viable count be not less than 5,000,000,000/
G, yeast viable count is not less than 20,000,000,000/g, and viable count of lactobacillus is not less than 10,000,000,000/g.
7. method according to claim 1 or 5, it is characterised in that：Composite bacteria is 1%-2%'s first with mass percent
G/W is added in mixed material after being recovered.
8. method according to claim 1, it is characterised in that：At 30-45 DEG C, fermentation time is that 70-75 is small to fermentation temperature
When.
9. method according to claim 1, it is characterised in that：Material moisture after drying is controlled after 10%-12%, crushing
Cross 40-80 mesh.
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| CN114304378A (en) * | 2021-12-28 | 2022-04-12 | 广州播恩维生素有限公司 | Biological fermentation method for improving corn DDGS water-soluble protein |
| CN116676203A (en) * | 2023-06-08 | 2023-09-01 | 珠海盈盛联科生物科技有限公司 | Preparation process of amino acid cereal yeast applied to skin care product |
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Application publication date: 20170531 |