CN100390268C - Artificial cultivated strain of tianshan Mountain wild mushroom and cultivation method therefor - Google Patents
Artificial cultivated strain of tianshan Mountain wild mushroom and cultivation method therefor Download PDFInfo
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- CN100390268C CN100390268C CNB2005100532381A CN200510053238A CN100390268C CN 100390268 C CN100390268 C CN 100390268C CN B2005100532381 A CNB2005100532381 A CN B2005100532381A CN 200510053238 A CN200510053238 A CN 200510053238A CN 100390268 C CN100390268 C CN 100390268C
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Abstract
The present invention discloses a mushroom strain which is suitable for artificial cultivation and a cultivation method thereof. The wild mushrooms collected from the northern slope of Tian mountain in Xinjinag are used as specimens. The specimens are domesticated and purified by the tissue separation method. When domesticated, the strain is taken for mushroom experiments under various bad conditions to measure that the stain has the maximum tolerance in various environment conditions, and various environment conditions have influence on strain growth and development; thus, the strain has strong resistant performance and stable genetic performance. Thus, the strain which maintains the special taste of the wide mushrooms can be planted in outside idle lands, forest belts, moo-cow yards and vegetable fields. The cultivation method mainly provides a culture medium and a preparation of cultivation materials which are suitable for the strain; the preparation obviously accelerates the growth and the development of strain filament greatly and increases the productivity of mushrooms. The method which has the advantages of low investment and high productivity is a new way of making money for farmers.
Description
One, technical field
The present invention relates to novel bacterial and the cultivating method thereof of a kind of edible mushrooms, the biology of this bacterial classification divides the generic Mycophyta, Basidiomycetes, Agaricales, Agaricaceae Agaricus.
Two, technical background
In Xinjiang of China along on the vast forest and grassland of Northern Slope of Tianshan Mountains band, a kind of wild mushroom of growing, it is not only big, meat is thick, have a kind of strong unique perfume, taste is very delicious, but also is rich in multivitamin and nutritive ingredient, have anticancer, hypotensive, the hypoglycemic medical functions that waits.But because its output is few, can not form commodity, so for a long time, be unknown to the external world.Particularly in recent years, along with the degeneration on grassland, the increase of carring capacity and artificial indiscriminate mining and serious waste, made the edge that this rare resource is endangered.At this situation, agricultural school, Hami organizes the teaching and scientific research personnel to carry out the domestication's breeding of Tianshan Mountains wild mushroom and the research of cultivation from the spring in 2003, and having obtained phasic results, this research has been applied for Xinjiang Uygur Autonomous Regions's natural science fund project and has been got permission project verification.Entrusting academy of agricultural sciences, Xinjiang rural economy and Scientific and Technical Information Institute to set up the project when the application project verification looks into newly, look into new conclusion and be " at home in the document; do not see that (Agaricus campstris) wild strain that possesses mushroom separates; be fit to that tame culture medium prescription screens and the closely related bibliographical information of domestication's technical essentials such as mushroom strain seed selection, the bibliographical information of similar research is not also seen in Xinjiang ".
Three, summary of the invention
The purpose of this invention is to provide a kind of is sample with the Tianshan Mountains wild mushroom, serves as female method of planting culturing edible fungus through artificial culture, the mushroom strain that forms of domestication and with this bacterial classification.This bacterial classification not only has the fragrance and the nutritive ingredient of Tianshan Mountains wild mushroom, and resistance, and anti-assorted bacterium property is strong, and heritability is stable, is suitable for artificial culture.Artificial cultivated strain of tianshan Mountain wild mushroom of the present invention is a new bacterial strain of mushroom kind of (Agaricus) Agaricus (Agaricus campestris), its taxonomy called after mushroom (Agaricus campestris) sky A1 number.This bacterial classification is deposited at Chinese typical culture collection center C CTCC on December 22nd, 2004, and preserving number is CCTCC NO M204101.With the method for above-mentioned artificial cultivated strain of tianshan Mountain wild mushroom culturing edible fungus, be to do female the kind with above-mentioned bacterial classification, cultivate original seed and cultivar, again cultivar is seeded on the cultivation material for preparing, through sending out a bacterium, management of producing mushroom, at last to plucking mushroom.Employed substratum when cultivating original seed and cultivar with female kind is to have added female kind of sample bacterial strain root soil water cooking liquid in conventional excrement grass substratum.
The pH value of above-mentioned soil water cooking liquid preferably and substratum be consistent.Above-mentioned culture material mainly is made up of carbon source, nitrogenous source and auxiliary material, and wherein carbon source mainly comprises agricultural crop straw and cotton seed hullss such as wheat straw, straw, corn, sunflower, cotton; Nitrogenous source mainly comprises the ight soil of animals such as ox, sheep, horse, donkey, pig, chicken etc., and various carbon sources and nitrogenous source are comprehensively arranged in pairs or groups, and contained carbon-nitrogen ratio should be 30-33 in back carbon source and the nitrogenous source: 1.
In the place that Herba Ephedrae is arranged, the carbon source in the cultivation material can add Herba Ephedrae (pharmaceutical factory has extracted the stalk tankage behind the racephedrine), and is delicious outside the mushroom taste Doug of planting out like this, and anti-assorted bacterium ability is strong.
Adopted technique scheme, because the cultivation of artificial cultivated strain of tianshan Mountain wild mushroom success, make endangered Tianshan Mountains wild mushroom realize artificial culture, secondly owing in breeding process, taked bacterial classification is placed on and done the fruiting test under the mal-condition, measure the influence that bacterial classification is grown growth with each environmental factors the maximum tolerance of various environmental factorss, by contrast, filter out resistance and resist assorted bacterium ability strong, the more stable bacterial classification of heritability is done female the kind.Because resistance and anti-assorted bacterium ability are strong, so this bacterial classification not only can be cultivated at warmhouse booth, also can utilize outdoor public land, old vacant room room, the forest belt, the fence grassland, the cattle and sheep circle, the vegetable plot, the interplanting of potato ditch, but planting type bedstead cultivation, but also level land ridge cultivation, these cultivation modes liquid manure out of question, do not account for or account for less the arable land, extensive management, less investment is because heritability is stable, so mushroom that artificial culture goes out, can keep former wild mushroom flavor, the heritable variation and the degeneration that also can significantly reduce novel bacterial are done in nutrition etc. like this, in addition, owing in substratum, add female kind sample root soil water cooking liquid, and carbon-nitrogen ratio, pH value can both be consistent with original hase, has promoted sprouting and the growth of mycelia, increase cultivating rate, improved yield and quality.
Four, embodiment
The invention will be further described below in conjunction with embodiment and embodiment:
Artificial cultivated strain of tianshan Mountain wild mushroom is Agaricus (Agaricus), the new bacterial strain of mushroom kind of (Agaricus campestris), and it is to gather Xinjiang Tianshan north slope wild mushroom to make sample, adopts tissue isolation through domestication, purification forms.
In breeding domestication process, bacterial classification is placed under the various environment, particularly be placed on than doing the fruiting test under the rugged environment (for example various near ultimate temperature, humidity, potential of hydrogen, illumination, ventilation, assorted bacterium etc.), determine the influence that bacterial classification is grown growth maximum tolerance and each environmental factors of various environmental factorss, thereby select resistance and resist assorted bacterium ability strong, the more stable bacterial strain of heritability is done female the kind.
Embodiment one: original seed, cultivar culture medium preparation
60 kilograms of cotton seed hullss, do 30 kilograms of cow dungs, 3 kilograms of terra albas, 8 kilograms in wheat bran, 1.5 kilograms in lime carbonate, earlier cow dung is smashed to pieces and adds water after mixing with cotton seed hulls, gypsum, wheat bran, lime carbonate and stir, again mother is planted sample bacterial strain root soil with water boil after, water cooking liquid is added in the compound, total water content is adjusted to 55%-60%, pH value transfers to 7.5-8, and (pH value should be consistent with mother culture media) gets final product after fermentation.
Embodiment two: plant formulation 1 (calculating by 100 square metres of plantation areas) (as follows)
Do 1000 kilograms of cow dungs, 500 kilograms of horsehits, 1000 kilograms of dried wheat straws (above carbon-nitrogen ratio is 33: 1), 25 kilograms of calcium superphosphate, 10 kilograms in urea, 25 kilograms in gypsum, 20 kilograms in lime, water content 55-60%.
Earlier that the excrement grass meal is broken, use water-soaked, to add a cover plastics and educate heap, turning five times during turning adds other auxiliary material, again through thermophilic fermentation, adjusts moisture content to 55-60%, and pH value 7.5-8 gets final product.
Embodiment three: plant formulation 2
Do 1200 kilograms of cow dungs, 300 kilograms of chicken manures, 800 kilograms of dried wheat straws, 100 kilograms of cottonseeds, 100 kilograms of the Herba Ephedrae dregs of a decoction (above carbon-nitrogen ratio is 30: 1), 10 kilograms in urea, 25 kilograms in gypsum, 20 kilograms in lime, 25 kilograms of calcium superphosphate, compound method is with embodiment 2.
Embodiment four: plant formulation 3
Do 1000 kilograms of cow dungs, 500 kilograms of pig manures, 500 kilograms of cotton seed hullss, 500 kilograms of dried wheat straws, (above carbon-nitrogen ratio is 32: 1), 10 kilograms in urea, 25 kilograms in gypsum, 20 kilograms in lime, 25 kilograms of calcium superphosphate.Compound method is with embodiment 3.
Except above-mentioned embodiment, the preparation of breeding, cultivating material of other technology such as bacterial classifications at different levels, fermentation and sterilization, sowing, management, fruitings etc. all are same as existing known technology, repeat no more herein.
Claims (5)
1. artificial cultivated strain of tianshan Mountain wild mushroom, it is characterized in that: it is the new bacterial strain of Agaricus (Agaricus) mushroom kind of (Agaricus campestris), this bacterial classification has been deposited at Chinese typical culture collection center C CTCC, and preserving number is CCTCC NOM204101.
2. method with the said artificial cultivated strain of tianshan Mountain wild mushroom culturing edible fungus of claim 1, it is as mother's kind with above-mentioned bacterial classification, cultivate original seed and cultivar, again cultivar is seeded on the cultivation material for preparing, through sending out a bacterium, management of producing mushroom, at last to plucking mushroom, it is characterized in that: employed substratum when cultivating original seed and cultivar with female kind is to have added female kind of sample bacterial strain root soil water cooking liquid in conventional excrement grass substratum.
3. according to the said method with the artificial cultivated strain of tianshan Mountain wild mushroom culturing edible fungus of claim 2, it is characterized in that: said female pH value of planting sample bacterial strain root soil water cooking liquid should be consistent with substratum.
4. according to the said method with the artificial cultivated strain of tianshan Mountain wild mushroom culturing edible fungus of claim 2, it is characterized in that: the ratio of carbon and nitrogen element is 30～33: 1 in the said cultivation material.
5. according to the said method of claim 2, it is characterized in that: include pharmaceutical factory's Chinese ephedra tankage in the said cultivation material with the artificial cultivated strain of tianshan Mountain wild mushroom culturing edible fungus.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100532381A CN100390268C (en) | 2005-02-17 | 2005-02-17 | Artificial cultivated strain of tianshan Mountain wild mushroom and cultivation method therefor |
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| CNB2005100532381A CN100390268C (en) | 2005-02-17 | 2005-02-17 | Artificial cultivated strain of tianshan Mountain wild mushroom and cultivation method therefor |
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| CN1799316A CN1799316A (en) | 2006-07-12 |
| CN100390268C true CN100390268C (en) | 2008-05-28 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172168B (en) * | 2010-12-08 | 2012-07-25 | 中国科学院新疆理化技术研究所 | Method for separating, domesticating and cultivating wild Pholiota squarrosa strain |
| CN104620855B (en) * | 2015-03-11 | 2016-11-16 | 长春中医药大学 | Long handle oyster mushroom strain and culture medium, cultural method and application |
| CN105165411B (en) * | 2015-11-03 | 2018-08-10 | 哈密市庆丰设施农业科技开发有限公司 | The planting technique of wild orange mushroom |
| CN107926484A (en) * | 2017-12-05 | 2018-04-20 | 李博 | A kind of compost and its cultural method of straw rotting fungus ganoderma lucidum |
| CN108293599B (en) * | 2018-02-28 | 2020-05-19 | 中华全国供销合作总社昆明食用菌研究所 | Lepista sordida, and separation propagation method and soil-covering cultivation method thereof |
| CN111647512B (en) * | 2020-05-14 | 2023-02-14 | 新疆农业科学院植物保护研究所 | Isolated culture method of wild bird cherry mushroom strain |
| CN115349401B (en) * | 2022-09-16 | 2023-10-20 | 绵阳市农业科学研究院 | Domestication cultivation method for wild Pingwu mushrooms |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056528A (en) * | 1991-05-13 | 1991-11-27 | 山东农业大学 | Edible mushrooms ventilation tubular type bacterial classification and manufacturing and inoculation technique |
| CN1224059A (en) * | 1998-01-22 | 1999-07-28 | 李勇 | Process for producing nutrient granular edible fungus culture and its culture medium |
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2005
- 2005-02-17 CN CNB2005100532381A patent/CN100390268C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056528A (en) * | 1991-05-13 | 1991-11-27 | 山东农业大学 | Edible mushrooms ventilation tubular type bacterial classification and manufacturing and inoculation technique |
| CN1224059A (en) * | 1998-01-22 | 1999-07-28 | 李勇 | Process for producing nutrient granular edible fungus culture and its culture medium |
Non-Patent Citations (2)
| Title |
|---|
| 中天山林区野生食用真菌生态考察研究. 王俊燕，赵大明.生态学报，第17卷第4期. 1997 |
| 中天山林区野生食用真菌生态考察研究. 王俊燕，赵大明.生态学报，第17卷第4期. 1997 * |
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