CN100374037C - Sex gland mature promoting feed additive for tigar prawn - Google Patents
Sex gland mature promoting feed additive for tigar prawn Download PDFInfo
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Abstract
The present invention relates to a sex gland mature promoting feed additive for a tiger prawn, which is particularly to suitable for ripening the tiger prawn which achieve a mature age and an individual specification and is formed by components counted by weight parts as following: liquid height unsaturated fatty acid of 5 to 25 (wherein DHA+EPA is from 40.0 to 80.0%, and in addition, DHA/EPA is from 2.0 to 5.0), astaxanthin of five to ten with at least 1.5% of purity, cholesteryl of 5 to 10, vitamin C of 4 to 10, vitamin E of 1 to 3, sea tangle powder of 50 to 80 and other powder are sieved by a standard sieve with 100 meshes. The present invention can be well stuck to fresh food, such as squids, clam worms, etc., and the present invention and fresh food are together eaten by tiger parent prawns. The present invention can obviously promote the gonad development of artificial bred and wild tiger parent prawns, accelerate gonad mature, enhance the maturity and the fecundity of the parent prawns and notably enhance the mature ratio of a parent prawn group.
Description
Technical field
The present invention relates to a kind of feed addictive that promotes the Penaeus monodon gonad maturity, the accelerating the ripening of the Penaeus monodon that reaches maturation age and specification (body weight and body are long) that is particularly useful for propagating artificially.
Background technology
Shrimp culture is the pillar industry of China's culture fishery.Penaeus monodon (Penaeus monodon) is one of China's three big main cultured prawn kinds.At present, the seed of Penaeus monodon produces needed ripe parent and mainly relies on the sea to fish for natural population to obtain.Because the variation of wild Penaeus monodon stock number can't rely on and fish for the stable supply that guarantees the Penaeus monodon parent; Simultaneously, because white spot syndrome virus (White Spot Syndrome Virus, abbreviation WSSV) propagation, the ratio of carrying white spot syndrome virus in the WSSV natural reservoir (of bird flu viruses) in wild Penaeus monodon and the environment is very high, is difficult to guarantee the quality of wild Penaeus monodon parent; If can not artificial culture Penaeus monodon parent, just can't carry out the prevalent variety cultivation of Penaeus monodon.In this case, some countries begin the research carrying out the Penaeus monodon whole artificial propagation and grow seedlings, and obtain certain progress.But because at present to the grasp not fully aware of yet of Penaeus monodon breed mechanism, the Penaeus monodon parent shrimp maturing rate of propagating cultivation artificially is extremely low, the ripe ratio of the female shrimp of Penaeus monodon of artificial culture is difficult to reach large-scale production and application below 30%.Therefore, it is the important step of parent shrimp rearing with maturation that promotion or raising total man worker culture Penaeus monodon parent shrimp gonad development, is the key technology of Penaeus monodon whole artificial propagation.
Studies show that both at home and abroad nutrition is the key factor of decision Penaeus monodon parent's shrimp gonad development and reproductive capacity.Shrimps can not synthesize highly unsaturated fatty acid (as DHA and EPA) or synthesis capability very low, parent is grown the required highly unsaturated fatty acid bait of can only originating; Cholesterol, vitamin A, vitamin E, vitamin C and astaxanthin can both improve the fertility of close shrimp.Though a large amount of studies show that protein, amino acid, aliphatic acid, vitamin, trace elements are close shrimp gonad developments and breed required crucial trophic factors, but most research results is based on the nutritional labeling analysis to natural bait under the biochemical composition of close shrimp sexual gland different developmental phases and the natural conditions thereof, owing to being influenced by environment, weather etc., the nutritional labeling of natural bait changes, therefore, the effect and the Optimum Requirement of various Different Nutrition factor pair parent's shrimp gonad developments and breeding still be can not determine.
For other to shrimps, particularly the close shrimp nutrient research of Environment of Litopenaeus vannamei Low (Litopenaeus vannamei Boone) and close shrimp mixed feed and nutrient reinforcer for shrimp have been obtained some progress, as Chinese patent application number 02115067.2: granular shrimp feed, it comprises: (weight %) squid viscera powder 3.0～28.0, white fish meal 15.0～55.0, shrimp chaff powder 1.0～10.0, flour 10.0～25.0, granular shrimp feed specialist additive 10.0～45.0, phosphatidase 10 .2～2.5, fish oil 2.0～8.0, vitamin mixtures 0.5～3.0, mineral salt mixture 0.5～3.0 and calcium dihydrogen phosphate 0.2～2.0.Also has Chinese patent application numbers 200410077219.8: a kind of nutrient reinforcer for shrimp, it is characterized in that by mass fraction, by vitamin mixtures 5.0%～50.0%, cholesterol 1.0%～20.0%, mineral salt mixture 5.0%～35.0%, immune polysaccharide 0.5%～12.0%, sodium alginate 3.0%～25.0%, astaxanthin 0.5%～10.0% and Choline Chloride 1.0%～15.0% are formed.Mentioned close shrimp is a kind of definition widely in the aforementioned patent, and its concrete objective for implementation is an Environment of Litopenaeus vannamei Low.Studies show that different types of close shrimp nutritional need is also inconsistent, is subjected to the influence of factors such as life habit, individual size very big, above-mentioned mixed feed and nutrient reinforcer for shrimp are not remarkable to promotion Penaeus monodon gonad development, raising gonad maturity rate effect.
Penaeus monodon is that all cultured prawn kinds are individual maximum, the sexal maturity individuality of its natural close shrimp is much larger than its prawn kind, its granular size to bait, palatability, nutrition etc. all have certain specific (special) requirements, and not liking anthropophagy worker's pellet is the reason that artifical compound feed is difficult to use on the Penaeus monodon parent shrimp rearing.Therefore, do not see the application of granular mixed feed so far as yet artificial culture Penaeus monodon parent shrimp.Although Marsden etc. (1997) replace natural baits such as squid and mussel fully with a kind of soft pellet diet, can improve lay eggs number of times and the nauplius survival rate of Penaeus monodon parent shrimp, but its employed feed mainly with fresh and alive biotic component preparation, still can not be referred to as real mixed feed.Owing to can't obtain a kind of desirable artificial close shrimp mixed feed, at present, the Penaeus monodon parent shrimp rearing still can only rely on the fresh food of throwing something and feeding to guarantee the nutrition supply of its breeding period.
Summary of the invention
The object of the present invention is to provide a kind of parent feed addictive that promotes the Penaeus monodon gonad development, improves the gonad maturity rate.
The present invention also provides the preparation method and the using method of this additive.
The present invention is made of following component in parts by weight:
Highly unsaturated fatty acid 5-25; The astaxanthin 5-10 of purity more than 1.5%;
Cholesterol 5-10; Vitamin C 4-10;
Vitamin e1-3; Sea-tangle powder 50-80;
Described highly unsaturated fatty acid is liquid, and wherein DHA (DHA) adds eicosapentaenoic acid (EPA) and accounts for 40.0～80.0% of weight, and DHA/EPA is between 2.0～5.0; Its preferable range: DHA+EPA accounts for the 50.0-80.0% of weight, and DHA/EPA is between 2.0～4.0; Special preferable range: DHA+EPA accounts for the 60.0-75.0% of weight, and DHA/EPA is between 3.0～4.0;
Described all the other components all pass through 100 purpose standard screens.
The preparation method that Penaeus monodon of the present invention accelerates the ripening feed addictive at first weighs respectively by above-mentioned prescription, all pulvis was stirred in mixer 15 minutes, and then add highly unsaturated fatty acid continuation stirring 15 minutes.
Feed addictive of the present invention adopts vacuum packaging, every bag of 500g.Be suitable for low temperature, lucifuge is deposited, the shelf-life is 3 months.After opening, once do not use up, must and deposit in-20 ℃ the refrigerator-freezer with bag film sealing.
Feed addictive of the present invention and close shrimp are accelerated the ripening after the bait commonly used of process such as squid, clam worm etc. mix, and can adhere to squid, clam worm surface well, are together ingested by the Penaeus monodon parent shrimp in cultivating with squid, clam worm etc.
Beneficial effect of the present invention: the present invention--promote the application of the feed addictive of Penaeus monodon parent shrimp gonad maturity, can obviously promote to propagate artificially gonad development with wild Penaeus monodon parent shrimp, the acceleration gland maturity, improve the ripe ratio of maturity, egg laying amount and the colony of close shrimp, significantly improve the percentage of ripened grains of close shrimp integral body, experiment effect is seen below and is stated comparative example.Solved total man worker and cultured Penaeus monodon parent shrimp gonad development and ripe key technology, thereby for Penaeus monodon whole artificial propagation and production application lay a good foundation, solved present close shrimp shortage and cause the expensive difficult problem of close shrimp, and the reduction seedling cost.And product component of the present invention is easy to buy, and is easy and simple to handle; Can also together be ingested with parent fresh food adhesions such as squid, clam worms well by Penaeus monodon parent shrimp.
The specific embodiment
The present invention relates to a kind ofly can promote Penaeus monodon parent shrimp gonad development and feed addictive ripe and that lay eggs, and improve the sexal maturity ratio of close shrimp integral body.The invention provides the production method of this feed addictive, and with its using method as Penaeus monodon parent shrimp fresh food additive.In addition, the artificial cooperation Penaeus monodon parent shrimp feed that comprises this feed addictive also is a content protion of the present invention.
Feed addictive of the present invention comprises sea-tangle powder, astaxanthin, highly unsaturated fatty acid EPA, DHA, vitamin C, vitamin E, cholesterol.The present invention is that a kind of fill-in of the natural bait use as Penaeus monodon parent shrimp joins in the fresh food, to remedy the deficiency of natural fresh food nutritional labeling, satisfies the special dietary needs of the close shrimp of Penaeus monodon in breeding period.
Although following term is widely known by the people in the art, for the ease of understanding, we will carry out some brief explanation with these terms.
Herein, " feed addictive " is meant by prescription of the present invention, with all the components after stirring abundant mixing, can directly mix with fresh food, finely ingested by Penaeus monodon parent shrimp, have and promote the Penaeus monodon parent ripe in the indoor culture pond, improve the nutrition fortifier of ripe ratio of maturity, egg laying amount and the colony of close shrimp.
Herein, " Penaeus monodon parent shrimp " be meant under field conditions (factors) or the artificial culture condition under, growth reaches a dating and individual specification, the ripe and prawn individuality that can produce offspring of attainability.
Herein, " gonad development " is meant that the development of ovary of female close shrimp is not from observing phase change in appearance to the partial shape that can observe ovary in appearance; The seminal vesicle of male close shrimp is not from observing phase change in appearance to the partial shape that can observe seminal vesicle in appearance.The development of ovary that " sexal maturity " is meant female close shrimp is obviously observed the full shape of ovary in appearance to IV more than the phase; The seminal vesicle of male close shrimp is grown the obviously seminal vesicle shape of visible white of outward appearance.
The present invention is described in further detail below by following examples.
Embodiment 1:
Make by following component: (in parts by weight)
Highly unsaturated fatty acid 10; The astaxanthin 7 of purity 1.5%;
Cholesterol 5; Vitamin C 5;
Vitamin e1; Sea-tangle powder 72;
Described highly unsaturated fatty acid is liquid, and wherein DHA (DHA) adds the 50.0-80.0% that eicosapentaenoic acid (EPA) accounts for weight, and DHA/EPA is between 2.0～4.0; All the other components all pass through 100 purpose standard screens.
Take by weighing various nutriments by above-mentioned umber, all pulvis were stirred in mixer 15 minutes, and then add highly unsaturated fatty acid continuation stirring 15 minutes, obtain Penaeus monodon parent shrimp accelerating feed addictive behind the mixing.The gained additive by every bag of 500g, after the vacuum-packed sealing, is reinstalled in the specified packet tinning, be positioned over-20 ℃ of refrigerators and preserve.
Embodiment 2:
According to claim, in parts by weight, by
Highly unsaturated fatty acid 20; Astaxanthin (purity 1.5%) 7;
Cholesterol 5; Vitamin C 5;
Vitamin e1; Sea-tangle powder 62;
Described highly unsaturated fatty acid is liquid, and wherein DHA+EPA accounts for the 60.0-75.0% of weight, and DHA/EPA is between 3.0～4.0;
Described all the other components all pass through 100 purpose standard screens.
Take by weighing various nutriments by above-mentioned umber, all pulvis were stirred in mixer 15 minutes, and then add highly unsaturated fatty acid continuation stirring 15 minutes, obtain Penaeus monodon parent shrimp accelerating feed addictive behind the mixing.Every bag of 500g after the vacuum-packed sealing, reinstalls in the specified packet tinning, is positioned over-20 ℃ of refrigerators and preserves.
Embodiment 3:
Make by following component: (in parts by weight)
Highly unsaturated fatty acid 5; The astaxanthin 5 of purity 1.5%;
Cholesterol 5; Vitamin C 4;
Vitamin e1; Sea-tangle powder 50;
Described highly unsaturated fatty acid is liquid, and wherein DHA (DHA) adds eicosapentaenoic acid (EPA) and accounts for 40.0～80.0% of weight, and DHA/EPA is between 2.0～5.0;
Described all the other components all pass through 100 purpose standard screens.
Embodiment 4:
Make by following component: (in parts by weight)
Highly unsaturated fatty acid 25; The astaxanthin 10 of purity 1.5%;
Cholesterol 10; Vitamin C 10;
Vitamin E 3; Sea-tangle powder 80;
Described highly unsaturated fatty acid is liquid, and wherein DHA (DHA) adds eicosapentaenoic acid (EPA) and accounts for 40.0～80.0% of weight, and DHA/EPA is between 2.0～5.0;
Described all the other components all pass through 100 purpose standard screens.
The test effect experiment:
The preparation fresh food: the fresh and alive or ice fresh bait material of buying from market is through artificial treatment, and ice Fresh squid removes its ink sac and internal organ, and squid is cut into long 2.0～4.0cm, and wide 0.5～1.0cm small pieces are clean with clean seawater flushing, and dried 10～15 minutes of filter is standby.The shellfish such as razor clam of hanging need be removed its shell, and is clean with clean seawater flushing, filter do 10～15 minutes standby.Living clam worm need be clean with clean seawater flushing, and dried 5～10 minutes of filter is standby.
Comparative example 1:
With embodiment 1 gained additive, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir the Penaeus monodon parent shrimp of throwing something and feeding.Check the experimental design of close shrimp accelerating feed addictive effect: from reaching or near the ripe pond culture reserve Penaeus monodon parent shrimp, in the mode that shrimp pot is traped and trawlnet is caught, selecting specification reaches: the long 16.0～18.0cm of female shrimp body, body weight 60.0～85.0g, long 13.5～the 15.0cm of male shrimp body, body weight 35.0～50.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, support after 10 days temporarily, carry out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 40 tails and the male shrimp (the male and female ratio is 2: 1) of 20 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 40 tails and the male shrimp (the male and female ratio is 2: 1) of 20 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 55.8 ± 3.7%, maturing rate 48.3 ± 4.2%, the total amount 2272.3 ten thousand of laying eggs, shrimp 101 tails of always laying eggs are inferior, and average every tail is laid eggs 225,000 at every turn, total nauplius is several 1433.6 ten thousand, average incubation rate 63.1 ± 4.6%; Male shrimp maturing rate 72.5 ± 5.1%.The female shrimp gonad development of control group Penaeus monodon ratio is 18.6 ± 2.4%, maturing rate 10.0 ± 2.5%, the total amount 223.2 ten thousand of laying eggs, shrimp 12 tails of always laying eggs are inferior, and average every tail is laid eggs 186,000 at every turn, total nauplius is several 102.9 ten thousand, average incubation rate 46.1 ± 4.3%; Male shrimp maturing rate 56.5 ± 4.7%.
Comparative example 2:
With embodiment 2 gained additives, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir the Penaeus monodon parent shrimp of throwing something and feeding.Check the experimental design of close shrimp accelerating feed addictive effect: from reaching or near the ripe pond culture reserve Penaeus monodon parent shrimp, in shrimp pot trapping mode, selecting specification reaches: the long 16.0～18.0cm of female shrimp body, body weight 60.0～85.0g, long 13.5～the 15.0cm of male shrimp body, body weight 35.0～50.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, support after 10 days temporarily, carry out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 40 tails and the male shrimp (the male and female ratio is 2: 1) of 20 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 40 tails and the male shrimp (the male and female ratio is 2: 1) of 20 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 64.5 ± 6.4%, maturing rate 57.5 ± 4.4%, the total amount 3422.4 ten thousand of laying eggs, shrimp 138 tails of always laying eggs are inferior, and average every tail is laid eggs 248,000 at every turn, total nauplius is several 2056.9 ten thousand, average incubation rate 60.1 ± 5.1%; Male shrimp maturing rate 76.5 ± 6.8%.The female shrimp gonad development of control group Penaeus monodon ratio is 14.7 ± 2.4%, maturing rate 9.0 ± 2.5%, the total amount 192.5 ten thousand of laying eggs, shrimp 11 tails of always laying eggs are inferior, and average every tail is laid eggs 175,000 at every turn, total nauplius is several 96.3 ten thousand, average incubation rate 50.1 ± 4.0%; Male shrimp maturing rate 60.5 ± 4.3%.
Comparative example 3:
With embodiment 1 gained additive, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir the Penaeus monodon parent shrimp of throwing something and feeding.Check the experimental design of close shrimp accelerating feed addictive effect, from reaching or near the ripe pond culture reserve Penaeus monodon parent shrimp, in shrimp pot trapping mode, select specification and reach the long 18.0cm of female shrimp body, more than the body weight 80.0g, the long 14.5～15.8cm of male shrimp body, body weight 45.0～60.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, supports after 10 days temporarily, carries out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 30 tails and the male shrimp (the male and female ratio is 2: 1) of 15 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 30 tails and the male shrimp (the male and female ratio is 2: 1) of 15 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 85.6 ± 5.3%, maturing rate 80.0 ± 4.4%, the total amount 3538.0 ten thousand of laying eggs, shrimp 116 tails of always laying eggs are inferior, and average every tail is laid eggs 305,000 at every turn, total nauplius is several 2476.7 ten thousand, average incubation rate 70.3 ± 5.1%; Male shrimp maturing rate 74.5 ± 6.3%.The female shrimp gonad development of control group Penaeus monodon ratio is 21.5 ± 2.4%, maturing rate 16.2 ± 3.0%, the total amount 459.8 ten thousand of laying eggs, shrimp 19 tails of always laying eggs are inferior, and average every tail is laid eggs 242,000 at every turn, total nauplius is several 220.7 ten thousand, average incubation rate 48.1 ± 3.3%; Male shrimp maturing rate 62.5 ± 3.6%.
Comparative example 4:
With example 2 gained additives, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir, and throws something and feeds to Penaeus monodon parent shrimp.Check the experimental design of close shrimp accelerating feed addictive effect, from reaching or near the ripe pond culture reserve Penaeus monodon parent shrimp, to throw net or shrimp pot trapping mode, select specification and reach the long 18.0cm of female shrimp body, more than the body weight 80.0g, the long 14.5～15.8cm of male shrimp body, about body weight 45.0～60.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, supports after 10 days temporarily, carries out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 30 tails and the male shrimp (the male and female ratio is 2: 1) of 15 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 30 tails and the male shrimp (the male and female ratio is 2: 1) of 15 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 89.6 ± 4.1%, maturing rate 86.0 ± 4.4%, the total amount 4705.8 ten thousand of laying eggs, shrimp 138 tails of always laying eggs are inferior, and average every tail is laid eggs 341,000 at every turn, total nauplius is several 2978.8 ten thousand, average incubation rate 68.3 ± 5.1%; Male shrimp maturing rate 77.5 ± 5.7%.The female shrimp gonad development of control group Penaeus monodon ratio is 26.7 ± 2.4%, maturing rate 18.0 ± 3.5%, the total amount 470.0 ten thousand of laying eggs, shrimp 20 tails of always laying eggs are inferior, and average every tail is laid eggs 235,000 at every turn, total nauplius is several 235.2 ten thousand, average incubation rate 50.1 ± 4.3%; Male shrimp maturing rate 60.5 ± 4.2%.
Comparative example 5:
In order to verify the accelerating effect of close shrimp accelerating feed addictive, carried out following test again to natural wild close shrimp.With example 1 gained additive, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir, and wild Penaeus monodon parent shrimp is caught in the sea of throwing something and feeding.Check the experimental design of close shrimp accelerating feed addictive effect, from the purchase South Sea, Sanya, Hainan northern wild Penaeus monodon parent shrimp, select specification and reach the long 18.0cm of female shrimp body, more than the body weight 80.0g, the long 15.0cm of male shrimp body, more than the body weight 50.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, support after 5～10 days temporarily, carry out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 20 tails and the male shrimp (the male and female ratio is 2: 1) of 10 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 20 tails and the male shrimp (the male and female ratio is 2: 1) of 10 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 76.5 ± 6.3%, maturing rate 70.6 ± 3.8%, the total amount 2911.0 ten thousand of laying eggs, shrimp 82 tails of always laying eggs are inferior, and average every tail is laid eggs 355,000 at every turn, total nauplius is several 2192.0 ten thousand, average incubation rate 75.3 ± 4.3%; Male shrimp maturing rate 84.5 ± 5.0%.The female shrimp gonad development of control group Penaeus monodon ratio is 38.0 ± 2.9%, maturing rate 36.0 ± 3.3%, the total amount 1113.0 ten thousand of laying eggs, shrimp 35 tails of always laying eggs are inferior, and average every tail is laid eggs 318,000 at every turn, total nauplius is several 713.5 ten thousand, average incubation rate 64.1 ± 3.7%; Male shrimp maturing rate 71.3 ± 5.3%.
Comparative example 6:
With example 2 gained additives, according to each food ration of close shrimp, the ratio of adding 50g parent shrimp accelerating feed addictive in the per kilogram fresh food joins close shrimp accelerating feed addictive in the fresh food and to stir, and wild Penaeus monodon parent shrimp is caught in the sea of throwing something and feeding.Check the experimental design of close shrimp accelerating feed addictive effect, from the purchase South Sea, Sanya, Hainan northern wild Penaeus monodon parent shrimp, select specification and reach the long 18.0cm of female shrimp body, more than the body weight 80.0g, the long 15.0cm of male shrimp body, more than the body weight 50.0g, individual uniform close shrimp is transported the parent shrimp rearing chamber back, support after 5～10 days temporarily, carry out grouping experiment.Before the grouping, every tail is tested female shrimp excise one-sided optic stalk.Experimental group comprises female shrimp of 20 tails and the male shrimp (the male and female ratio is 2: 1) of 10 tails, and three parallel laboratory test groups are established in experiment, throw something and feed and add the fresh food of close shrimp accelerating feed addictive.Control group comprises female shrimp of 20 tails and the male shrimp (the male and female ratio is 2: 1) of 10 tails, establishes three parallel group equally, the standby fresh food of directly throwing something and feeding.Experimental group and control group breeding condition are identical.Through reproduction test in 35 days, the female shrimp gonad development of experimental group Penaeus monodon ratio is 84.6 ± 4.1%, maturing rate 76.9 ± 4.4%, the total amount 3519.0 ten thousand of laying eggs, shrimp 90 tails of always laying eggs are inferior, and average every tail is laid eggs 391,000 at every turn, total nauplius is several 2978.8 ten thousand, average incubation rate 80.5 ± 5.7%; Male shrimp maturing rate 79.0 ± 6.3%.The female shrimp gonad development of control group Penaeus monodon ratio is 45.5 ± 3.5%, maturing rate 41.8 ± 3.0%, the total amount 1300.0 ten thousand of laying eggs, shrimp 40 tails of always laying eggs are inferior, and average every tail is laid eggs 325,000 at every turn, total nauplius is several 851.5 ten thousand, average incubation rate 65.5 ± 4.1%; Male shrimp maturing rate 70.5 ± 4.2%.
The above results shows, additive of the present invention is the accelerate the ripening additive of usefulness of a kind of extremely effectively Penaeus monodon parent shrimp, its effect comprises keeps fit Penaeus monodon parent shrimp, and promotes the gonad development, ripe and lay eggs of Penaeus monodon parent shrimp, significantly improves the percentage of ripened grains of pond adopted parents shrimp integral body.
The present invention is not limited in the scope of above-mentioned specific embodiment, and above-mentioned embodiment only is in order can be described in detail use of the present invention, and the production method of equal function and the part that composition also belongs to content of the present invention are arranged.In fact, those skilled in the art are according to the description of preamble, just can find a lot of different methods of adjustment according to needs separately, and these adjustment all should be in the scope of this paper institute appending claims.
Claims (4)
1. feed addictive that promotes the Penaeus monodon gonad maturity is made of following component in parts by weight:
Highly unsaturated fatty acid 10-20; The astaxanthin 5-10 of purity more than 1.5%;
Cholesterol 5-10; Vitamin C 4-10;
Vitamin e1-3; Sea-tangle powder 50-80;
Described highly unsaturated fatty acid is liquid, and wherein DHA DHA adds eicosapentaenoic acid EPA and accounts for 50.0～80.0% of weight, and DHA/EPA is between 2.0～5.0;
Described all the other components all pass through 100 purpose standard screens.
2. the feed addictive of promotion Penaeus monodon gonad maturity according to claim 1 is characterized in that: be made of following component in parts by weight:
Highly unsaturated fatty acid 10-20; The astaxanthin 6-8 of purity more than 1.5%;
Cholesterol 5-8; Vitamin C 4-8;
Vitamin e1-2; Sea-tangle powder 60-75;
Described highly unsaturated fatty acid wherein DHA DHA adds eicosapentaenoic acid EPA and accounts for 50.0～80.0% of weight, and DHA/EPA is between 2.0～4.0.
3. the feed addictive of promotion Penaeus monodon gonad maturity according to claim 1 is characterized in that: be made of following component in parts by weight:
Highly unsaturated fatty acid 10; The astaxanthin 7 of purity more than 1.5%;
Cholesterol 5; Vitamin C 5;
Vitamin e1; Sea-tangle powder 72;
Described highly unsaturated fatty acid wherein DHA DHA adds the 60.0-75.0% that eicosapentaenoic acid EPA accounts for weight, and DHA/EPA is between 3.0～4.0.
4. the feed addictive of promotion Penaeus monodon gonad maturity according to claim 1 is characterized in that: be made of following component in parts by weight:
Highly unsaturated fatty acid 20; The astaxanthin 7 of purity more than 1.5%;
Cholesterol 5; Vitamin C 5;
Vitamin e1; Sea-tangle powder 62;
Described highly unsaturated fatty acid wherein DHA DHA adds the 60.0-75.0% that eicosapentaenoic acid EPA accounts for weight, and DHA/EPA is between 3.0～4.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100371106A CN100374037C (en) | 2005-09-13 | 2005-09-13 | Sex gland mature promoting feed additive for tigar prawn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100371106A CN100374037C (en) | 2005-09-13 | 2005-09-13 | Sex gland mature promoting feed additive for tigar prawn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1739377A CN1739377A (en) | 2006-03-01 |
| CN100374037C true CN100374037C (en) | 2008-03-12 |
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| CNB2005100371106A Expired - Fee Related CN100374037C (en) | 2005-09-13 | 2005-09-13 | Sex gland mature promoting feed additive for tigar prawn |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100459876C (en) * | 2006-05-09 | 2009-02-11 | 广东恒兴集团有限公司 | An environment friendly compound feed for Litopenaeus vannamei parent shrimps |
| CN102265985B (en) * | 2011-04-02 | 2013-04-17 | 大连理工大学 | Feed additive for promoting ripening of sea cucumber seeds |
| CN102715379B (en) * | 2012-07-05 | 2014-04-09 | 广东粤佳饲料有限公司 | Compound shell reinforcing additive for sugpo prawns and preparation method of compound shell reinforcing additive |
| CN102835549A (en) * | 2012-08-27 | 2012-12-26 | 广东海大集团股份有限公司 | Compound feed special for penaeus monodon breeding season nutrient enrichment and preparation method thereof |
| CN105533266A (en) * | 2015-12-17 | 2016-05-04 | 宁波大学 | Feed additive capable of promoting development of ovaries and vitellogenesis of blue crabs and application of feed additive |
| CN107258622B (en) * | 2017-07-12 | 2019-09-27 | 中国水产科学研究院黄海水产研究所 | A kind of dietary methods of regulation Cynoglossus semilaevis parent population sex hormone secretion |
| CN107343579B (en) * | 2017-08-25 | 2021-08-24 | 中国水产科学研究院南海水产研究所深圳试验基地 | Earthworm is used as ripening bait for Penaeus monodon instead of clamworm and preparation method thereof |
| CN108576495A (en) * | 2018-04-18 | 2018-09-28 | 广东粤海饲料集团股份有限公司 | A kind of breeding additive of Litopenaeus vannamei Broodstock and its application |
| CN109122445B (en) * | 2018-08-27 | 2021-02-12 | 中国水产科学研究院黄海水产研究所 | Parent shrimp breeding method for improving breeding performance of litopenaeus vannamei |
| CN111109449A (en) * | 2020-01-14 | 2020-05-08 | 大连海洋大学 | Maturing-promoting compound feed for seed shrimps of prawns and preparation method thereof |
| CN112841455A (en) * | 2021-02-02 | 2021-05-28 | 广东恒兴饲料实业股份有限公司 | Special compound feed for breeding penaeus monodon parent shrimps and preparation method thereof |
| CN112841456B (en) * | 2021-02-23 | 2022-03-15 | 江苏海洋大学 | Shrimp compound feed for promoting gonad development of palaemon carinicauda and preparation method and application thereof |
| CN115736131B (en) * | 2022-10-27 | 2024-09-17 | 广东海大集团股份有限公司 | Penaeus vannamei Boone parent feed additive and preparation method and application thereof |
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| WO2004036982A2 (en) * | 2002-10-24 | 2004-05-06 | Advanced Bionutrition Corporation | Shrimp and the production thereof |
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| CN1620895A (en) * | 2004-12-10 | 2005-06-01 | 中国科学院南海海洋研究所 | Nutrient reinforcer for shrimp |
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| US20040203121A1 (en) * | 1988-09-07 | 2004-10-14 | Martek Biosciences Corporation | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| WO2004036982A2 (en) * | 2002-10-24 | 2004-05-06 | Advanced Bionutrition Corporation | Shrimp and the production thereof |
| CN1547935A (en) * | 2003-05-13 | 2004-11-24 | 上海帮侬生物技术有限公司 | Open-mouthed bait for raising aquatic fry and its production process |
| CN1620895A (en) * | 2004-12-10 | 2005-06-01 | 中国科学院南海海洋研究所 | Nutrient reinforcer for shrimp |
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