CN100343390C - 反义寡核苷酸的分离 - Google Patents

反义寡核苷酸的分离 Download PDF

Info

Publication number
CN100343390C
CN100343390C CNB2003801043610A CN200380104361A CN100343390C CN 100343390 C CN100343390 C CN 100343390C CN B2003801043610 A CNB2003801043610 A CN B2003801043610A CN 200380104361 A CN200380104361 A CN 200380104361A CN 100343390 C CN100343390 C CN 100343390C
Authority
CN
China
Prior art keywords
oligonucleotides
antisense oligonucleotide
antisense
thioated
resin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2003801043610A
Other languages
English (en)
Chinese (zh)
Other versions
CN1717484A (zh
Inventor
K·埃里克松
B·-L·约翰松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN1717484A publication Critical patent/CN1717484A/zh
Application granted granted Critical
Publication of CN100343390C publication Critical patent/CN100343390C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB2003801043610A 2002-11-28 2003-11-17 反义寡核苷酸的分离 Expired - Fee Related CN100343390C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE02035210 2002-11-28
SE0203521A SE0203521D0 (sv) 2002-11-28 2002-11-28 Isolation of antisense oligonucleotides

Publications (2)

Publication Number Publication Date
CN1717484A CN1717484A (zh) 2006-01-04
CN100343390C true CN100343390C (zh) 2007-10-17

Family

ID=20289700

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2003801043610A Expired - Fee Related CN100343390C (zh) 2002-11-28 2003-11-17 反义寡核苷酸的分离

Country Status (11)

Country Link
US (1) US7368561B2 (enExample)
EP (1) EP1578968B1 (enExample)
JP (1) JP2006507825A (enExample)
CN (1) CN100343390C (enExample)
AT (1) ATE325191T1 (enExample)
AU (1) AU2003279676B2 (enExample)
CA (1) CA2506196A1 (enExample)
DE (1) DE60305075T2 (enExample)
ES (1) ES2263038T3 (enExample)
SE (1) SE0203521D0 (enExample)
WO (1) WO2004048569A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100016564A1 (en) * 2006-05-30 2010-01-21 Ge Healthcare Bio-Sciences Ab Method of preparing an immobilised metal ion chromatography adsorbent and methods of purifying proteins, peptides or polynucleotides
JP2009073819A (ja) * 2007-08-29 2009-04-09 Fujifilm Corp 生理活性物質の精製方法
US8753868B2 (en) 2008-08-04 2014-06-17 General Electric Company Method and system for selective isolation of target biological molecules in a general purpose system
US7738454B1 (en) * 2008-09-30 2010-06-15 Juniper Networks, Inc. Methods and apparatus related to packet classification based on range values
CN110346493B (zh) * 2019-05-23 2021-01-05 中国人民解放军军事科学院军事医学研究院 检测猕猴血浆中中期因子反义寡核苷酸含量的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998000435A2 (en) * 1996-07-03 1998-01-08 President And Fellows Of Harvard College Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides
WO1999009045A1 (en) * 1997-08-20 1999-02-25 Somagenics, Inc. Antisense and antigene therapeutics with improved binding properties and methods for their use
WO2002046398A2 (en) * 2000-11-06 2002-06-13 The University Of Houston System Nucleic acid separation using immobilized metal affinity chromatography

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998000435A2 (en) * 1996-07-03 1998-01-08 President And Fellows Of Harvard College Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides
WO1999009045A1 (en) * 1997-08-20 1999-02-25 Somagenics, Inc. Antisense and antigene therapeutics with improved binding properties and methods for their use
WO2002046398A2 (en) * 2000-11-06 2002-06-13 The University Of Houston System Nucleic acid separation using immobilized metal affinity chromatography

Also Published As

Publication number Publication date
ATE325191T1 (de) 2006-06-15
CN1717484A (zh) 2006-01-04
ES2263038T3 (es) 2006-12-01
DE60305075T2 (de) 2006-12-14
AU2003279676A1 (en) 2004-06-18
AU2003279676B2 (en) 2009-01-22
US20060003331A1 (en) 2006-01-05
US7368561B2 (en) 2008-05-06
CA2506196A1 (en) 2004-06-10
WO2004048569A1 (en) 2004-06-10
JP2006507825A (ja) 2006-03-09
EP1578968A1 (en) 2005-09-28
SE0203521D0 (sv) 2002-11-28
EP1578968B1 (en) 2006-05-03
DE60305075D1 (de) 2006-06-08

Similar Documents

Publication Publication Date Title
JP4869247B2 (ja) 混合様式アニオン交換型分離材料
EP0797096A2 (en) Selective recognition of solutes in chromatographic media by artificially created affinity
JP5148484B2 (ja) クロマトグラフィーマトリックスの再生
JP2002529714A (ja) クロマトグラフィー分離法および選択吸着体
JP5826180B2 (ja) 分離マトリックス
US5801237A (en) Method for the purification of short nucleic acids
CN107001410A (zh) 混合床离子交换吸附剂
Yang et al. Immobilized iminodiacetic acid (IDA)‐type Cu2+‐chelating membrane affinity chromatography for purification of bovine liver catalase
WO2009003952A2 (en) Column and method for preparing a biological sample for protein profiling
CN100343390C (zh) 反义寡核苷酸的分离
US20090234033A1 (en) Separating Agent for IgG Purification and Method for Purifying an IgG Monomer by Using It
Sii et al. Bioseparation using affinity techniques
US7781573B2 (en) Multi layer chromatography of nucleic acids
EP1232006A1 (en) A method for selective removal of a substance from samples containing compounds having nucleic acid structure
JP2006523220A (ja) マルチモードアニオン交換リガンドの製造法
CN101119797A (zh) 液相色谱法
CN1639336A (zh) 利用芳香族硫醚配体分离的方法
EP3766573A1 (en) Chromatographic matrix, method and uses thereof
WO2009007993A4 (en) A process for purification of immunoglobulins using a pseudobioaffinity adsorbent
WO2022244890A1 (ja) ボロン酸アフィニティクロマトグラフィー用分離剤担体、カラム及びそれを用いた測定方法
US20090047734A1 (en) Method of separation of deoxyribonucleic acids
US20110152510A1 (en) Simple load and elute process for purification of genomic dna
Padmavathi et al. SEPERATION OF PROTEINS BY CHROMATOGRAPHIC TECHNIQUES
NARAYANAN Application of liquid Chromatography to the Purification of Proteins and Peptides
WO2017146225A1 (ja) アフィニティクロマトグラフィー用多孔質担体、リガンド結合多孔質担体、標的物の精製方法、及び抗体

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20071017

Termination date: 20101117