CL2021003555A1 - Dispositivos, sistemas y kits para suministro electromecánico y procedimiento de uso de los mismos. - Google Patents

Dispositivos, sistemas y kits para suministro electromecánico y procedimiento de uso de los mismos.

Info

Publication number
CL2021003555A1
CL2021003555A1 CL2021003555A CL2021003555A CL2021003555A1 CL 2021003555 A1 CL2021003555 A1 CL 2021003555A1 CL 2021003555 A CL2021003555 A CL 2021003555A CL 2021003555 A CL2021003555 A CL 2021003555A CL 2021003555 A1 CL2021003555 A1 CL 2021003555A1
Authority
CL
Chile
Prior art keywords
kits
systems
devices
procedure
electroporation zone
Prior art date
Application number
CL2021003555A
Other languages
English (en)
Inventor
Paulo Andres Garcia
Rameech Mccormack
Jessica Sido
James Hemphill
Harrison Bralower
Ross Beighley
Cullen Buie
Bethany Grant
Original Assignee
Kytopen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2019/058375 external-priority patent/WO2020087074A2/en
Application filed by Kytopen Corp filed Critical Kytopen Corp
Publication of CL2021003555A1 publication Critical patent/CL2021003555A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Electromagnetism (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Medicinal Preparation (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan dispositivos, sistemas y kits para la transfección celular. Un dispositivo incluye un primer electrodo, un segundo electrodo y una zona de electroporación entre estos donde una diferencia de potencial eléctrico aplicado al primer y al segundo electrodo genera un campo eléctrico en la zona de electroporación suficiente para transfectar al menos un subconjunto de las células en la vía de flujo. También se proporcionan métodos para introducir una composición en al menos una parte de una pluralidad de células con el uso de los dispositivos, sistemas y kits de la invención.
CL2021003555A 2019-07-02 2021-12-29 Dispositivos, sistemas y kits para suministro electromecánico y procedimiento de uso de los mismos. CL2021003555A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962869958P 2019-07-02 2019-07-02
PCT/US2019/058375 WO2020087074A2 (en) 2018-10-26 2019-10-28 Devices, systems, and kits for electroporation and methods of use thereof

Publications (1)

Publication Number Publication Date
CL2021003555A1 true CL2021003555A1 (es) 2022-08-19

Family

ID=74100196

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2021003555A CL2021003555A1 (es) 2019-07-02 2021-12-29 Dispositivos, sistemas y kits para suministro electromecánico y procedimiento de uso de los mismos.

Country Status (14)

Country Link
US (1) US20220243164A1 (es)
EP (1) EP3993900A4 (es)
JP (1) JP2022540797A (es)
KR (1) KR20220041101A (es)
CN (1) CN114340777A (es)
AU (1) AU2020299541A1 (es)
BR (1) BR112021026642A2 (es)
CA (1) CA3145294A1 (es)
CL (1) CL2021003555A1 (es)
CO (1) CO2022001001A2 (es)
EC (1) ECSP22007999A (es)
IL (1) IL289270A (es)
MX (1) MX2022000178A (es)
WO (1) WO2021003451A1 (es)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
DK2574662T3 (da) * 2001-08-22 2021-09-20 Maxcyte Inc Fremgangsmåde til elektroporation af biologiske prøver
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20070105206A1 (en) 2005-10-19 2007-05-10 Chang Lu Fluidic device
RU2656156C2 (ru) * 2011-10-17 2018-05-31 Массачусетс Инститьют Оф Текнолоджи Внутриклеточная доставка
EP2911736A4 (en) * 2012-10-25 2016-06-15 Oncosec Medical Inc electroporation
WO2016003485A1 (en) 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
WO2016077761A1 (en) * 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
BR112018004278B1 (pt) 2015-09-04 2022-05-03 Rutgers, The State University Of New Jersey Sistema para eletroporação de uma célula biológica, e método para eletroporação de células biológicas em uma solução tampão
US20190136224A1 (en) * 2016-05-31 2019-05-09 Massachusetts Institute Of Technology Hydrodynamically Controlled Electric Fields for High Throughput Transformation & High Throughput Parallel Transformation Platform
CN206109418U (zh) * 2016-08-31 2017-04-19 中国人民解放军第四军医大学 一种用于三维培养细胞电穿孔的实验装置
CN107486114B (zh) * 2017-09-22 2023-05-23 江南大学 基于感应电场的多磁路多次级流体反应系统及其应用

Also Published As

Publication number Publication date
JP2022540797A (ja) 2022-09-20
US20220243164A1 (en) 2022-08-04
CA3145294A1 (en) 2021-01-07
KR20220041101A (ko) 2022-03-31
MX2022000178A (es) 2022-05-18
ECSP22007999A (es) 2022-03-31
EP3993900A1 (en) 2022-05-11
AU2020299541A1 (en) 2022-01-27
WO2021003451A1 (en) 2021-01-07
CO2022001001A2 (es) 2022-04-29
BR112021026642A2 (pt) 2022-05-10
CN114340777A (zh) 2022-04-12
IL289270A (en) 2022-02-01
EP3993900A4 (en) 2023-10-18

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