CL2013003212A1 - Cepa de levadura kluyveromyces lactis, proteina obtenida de la cepa, vector y procedimientos de obtencion de azucares, etanol, b-galactosidasa y biomasa. - Google Patents

Cepa de levadura kluyveromyces lactis, proteina obtenida de la cepa, vector y procedimientos de obtencion de azucares, etanol, b-galactosidasa y biomasa.

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Publication number
CL2013003212A1
CL2013003212A1 CL2013003212A CL2013003212A CL2013003212A1 CL 2013003212 A1 CL2013003212 A1 CL 2013003212A1 CL 2013003212 A CL2013003212 A CL 2013003212A CL 2013003212 A CL2013003212 A CL 2013003212A CL 2013003212 A1 CL2013003212 A1 CL 2013003212A1
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CL
Chile
Prior art keywords
strain
biomass
vector
ethanol
procedures
Prior art date
Application number
CL2013003212A
Other languages
English (en)
Inventor
Rodriguez Angel Pereira
Fernandez Manuel Becerra
Siso Maria Isabel Gonzalez
Villanueva Maria Esperanza Cedran
Original Assignee
Queizuar S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Queizuar S L filed Critical Queizuar S L
Publication of CL2013003212A1 publication Critical patent/CL2013003212A1/es

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LE PRESENTE INVENCION SE REFIERE A UNA CEPA DE LEVADURA KLUYVEROMYCES FLACTIS DEPOSITADA EN LA COLECCIÓN DE CULTIVOS DEUTSCHE SRIMMLUNG VON MTKROORGANMSRNERI URID ZOLLKULTUREN GTT1BH (DSMZ) CON EL NÚMERO DE DEPÓSITO DSM 24900. QUE COMPRENDE LA SECUENCIA IDENTIFICADA POR SEQ ID NO 1 TAMBIÉN SE REFIERE A PROTEINA OBTENIDA DE LA CEPA DEPOSITADA, VECTOR Y PROCEDIMIENTOS DE OBTENCIÓN DE AZÚCARES, ETANOL, P.GALACTOSIDASA Y BIOMASA.
CL2013003212A 2011-06-24 2013-11-08 Cepa de levadura kluyveromyces lactis, proteina obtenida de la cepa, vector y procedimientos de obtencion de azucares, etanol, b-galactosidasa y biomasa. CL2013003212A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201131065A ES2397334B1 (es) 2011-06-24 2011-06-24 Cepa de levadura kluyveromyces lactis y procedimiento de obtención de azúcares, etanol, beta-galactosidasa y biomasa.

Publications (1)

Publication Number Publication Date
CL2013003212A1 true CL2013003212A1 (es) 2014-08-29

Family

ID=47422062

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013003212A CL2013003212A1 (es) 2011-06-24 2013-11-08 Cepa de levadura kluyveromyces lactis, proteina obtenida de la cepa, vector y procedimientos de obtencion de azucares, etanol, b-galactosidasa y biomasa.

Country Status (8)

Country Link
US (1) US9279135B2 (es)
EP (1) EP2725098B1 (es)
BR (1) BR112013028893A2 (es)
CL (1) CL2013003212A1 (es)
ES (2) ES2397334B1 (es)
MX (1) MX340809B (es)
PT (1) PT2725098T (es)
WO (1) WO2012175760A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3339433A1 (en) 2014-01-14 2018-06-27 DSM IP Assets B.V. Improved enzyme variants of lactase from kluyveromyces lactis
EP3300606A1 (de) 2016-09-28 2018-04-04 DMK Deutsches Milchkontor GmbH Aufschäumbare milchzubereitung
US11479763B2 (en) * 2017-04-07 2022-10-25 Dupont Nutrition Biosciences Aps Bacillus host cells producing β-galactosidases and lactases in the absence of p-nitrobenzylesterase side activity
MX2021006546A (es) * 2018-12-03 2021-08-24 Dupont Nutrition Biosci Aps Uso de lactasa para proporcionar un nivel alto de fibra de galactooligosacáridos (gos) y un nivel bajo de lactosa.
CN111334541A (zh) * 2020-02-24 2020-06-26 天津大学 一种β-半乳糖苷酶制备高纯度低聚半乳糖的方法
CN114456956A (zh) * 2022-04-14 2022-05-10 中义(北京)健康研究院 一种乳酸克鲁维酵母菌及其分离和培养方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2178431B (en) * 1985-07-15 1989-11-22 Bioteknika International Genetically engineered yeast strains
JP2009533058A (ja) * 2006-04-14 2009-09-17 ニユー・イングランド・バイオレイブス・インコーポレイテツド 変異体マルトース結合タンパク質に融合された標的タンパク質の可溶化及び精製
ES2319489B1 (es) * 2007-07-11 2010-02-10 Universidade Da Coruña Cepas de levadura capaces de secretar beta-galactosidasa al medio y su uso para la produccion de biomasa, etanol, beta-galactosidasa y proteinas de interes.
US20090253174A1 (en) * 2008-04-08 2009-10-08 Zach Serber Expression of Heterologous Sequences

Also Published As

Publication number Publication date
PT2725098T (pt) 2018-07-31
ES2397334A1 (es) 2013-03-06
WO2012175760A1 (es) 2012-12-27
US9279135B2 (en) 2016-03-08
ES2685786T3 (es) 2018-10-11
EP2725098A1 (en) 2014-04-30
US20150031078A1 (en) 2015-01-29
ES2397334B1 (es) 2014-06-06
EP2725098A4 (en) 2015-04-15
MX340809B (es) 2016-07-27
EP2725098B1 (en) 2018-05-30
MX2013013049A (es) 2014-05-21
BR112013028893A2 (pt) 2017-01-31

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