CL2012001644A1 - Método para determinar la cigocidad de un evento tc1507 en un tejido de zea mays, donde dicho evento tc 1507 comprende el constructo de un transgén que comprende un gen cry1f, y el cual comprende utilizar un ensayo de pcr taq de punto final basado en fluorescencia para detectar dicho evento tc1507; y un kit para poner en práctica dicho método. - Google Patents

Método para determinar la cigocidad de un evento tc1507 en un tejido de zea mays, donde dicho evento tc 1507 comprende el constructo de un transgén que comprende un gen cry1f, y el cual comprende utilizar un ensayo de pcr taq de punto final basado en fluorescencia para detectar dicho evento tc1507; y un kit para poner en práctica dicho método.

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Publication number
CL2012001644A1
CL2012001644A1 CL2012001644A CL2012001644A CL2012001644A1 CL 2012001644 A1 CL2012001644 A1 CL 2012001644A1 CL 2012001644 A CL2012001644 A CL 2012001644A CL 2012001644 A CL2012001644 A CL 2012001644A CL 2012001644 A1 CL2012001644 A1 CL 2012001644A1
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CL
Chile
Prior art keywords
event
zygosity
transgene
fluorescence
kit
Prior art date
Application number
CL2012001644A
Other languages
English (en)
Inventor
Wei Chen
Stephen Novak
Wesley Marchione
Manju Gupta
Thomas W Greene
Siva Kumpatla
Original Assignee
Dow Agrosciences Llc
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Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of CL2012001644A1 publication Critical patent/CL2012001644A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para determinar la cigocidad de un evento TC1507 en un tejido de Zea Mays, donde dicho evento TC1507 comprende el constructo de un transgén que comprende un gen Cry1F, y el cual comprende utilizar un ensayo de PCR TAQ de punto final basado en fluorescencia para detectar dicho evento TC1507; y un kit para poner en práctica dicho método.
CL2012001644A 2009-12-18 2012-06-18 Método para determinar la cigocidad de un evento tc1507 en un tejido de zea mays, donde dicho evento tc 1507 comprende el constructo de un transgén que comprende un gen cry1f, y el cual comprende utilizar un ensayo de pcr taq de punto final basado en fluorescencia para detectar dicho evento tc1507; y un kit para poner en práctica dicho método. CL2012001644A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12/642,352 US20110151441A1 (en) 2009-12-18 2009-12-18 Endpoint taqman methods for determining zygosity of corn comprising tc1507 events

Publications (1)

Publication Number Publication Date
CL2012001644A1 true CL2012001644A1 (es) 2012-11-30

Family

ID=44151631

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012001644A CL2012001644A1 (es) 2009-12-18 2012-06-18 Método para determinar la cigocidad de un evento tc1507 en un tejido de zea mays, donde dicho evento tc 1507 comprende el constructo de un transgén que comprende un gen cry1f, y el cual comprende utilizar un ensayo de pcr taq de punto final basado en fluorescencia para detectar dicho evento tc1507; y un kit para poner en práctica dicho método.

Country Status (15)

Country Link
US (1) US20110151441A1 (es)
EP (1) EP2513326A4 (es)
JP (1) JP2013514780A (es)
KR (1) KR20120107998A (es)
CN (1) CN102770553A (es)
AR (1) AR079520A1 (es)
AU (1) AU2010330806A1 (es)
BR (1) BR112012014929A2 (es)
CA (1) CA2783254A1 (es)
CL (1) CL2012001644A1 (es)
CO (1) CO6511270A2 (es)
MX (1) MX2012007136A (es)
RU (1) RU2012130431A (es)
WO (1) WO2011075648A1 (es)
ZA (1) ZA201204913B (es)

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US10214783B2 (en) * 2010-10-06 2019-02-26 Dow Agrosciences Llc Endpoint zygosity assay to detect Rf4 gene in maize
PT2768981T (pt) * 2011-10-21 2018-05-18 Dow Agrosciences Llc Método para determinar a zigosidade do gene fad2 em canola usando pcr end-point
CN104011225A (zh) * 2011-10-21 2014-08-27 陶氏益农公司 确定芸苔中fad3基因的接合性的方法
CN102618658B (zh) * 2012-04-18 2013-10-09 四川省农业科学院分析测试中心 转基因玉米tc1507品系特异定量pcr精准检测方法
BR112015017788A2 (pt) * 2013-01-25 2017-08-22 Pioneer Hi Bred Int Construto de dna, método para produzir uma planta, método de detecção da presença de uma molécula de ácido nucleico, par de iniciadores de polinucleotídio, evento de milho, método de detecção da presença do dna e kit para detectar ácidos nucleicos
EP3207156A1 (en) * 2014-10-13 2017-08-23 Life Technologies Corporation Methods, kits & compositions for determining gene copy numbers
CN104561366A (zh) * 2015-02-06 2015-04-29 曹际娟 利用微流体芯片高通量检测转基因玉米的方法
ES2905649T3 (es) 2015-06-24 2022-04-11 Syngenta Participations Ag Una molécula de ácidos nucleicos para conferir propiedades insecticidas a las plantas
CN108359717A (zh) * 2018-05-11 2018-08-03 上海市农业科学院 一种直扩rpa现场可视化检测方法
CN110564822B (zh) * 2019-09-20 2023-06-20 中国科学院成都生物研究所 基于LAMP技术的转基因玉米Bt176相关基因检测方法及试剂盒
CN110643734A (zh) * 2019-11-11 2020-01-03 上海海关动植物与食品检验检疫技术中心 一种转基因玉米das-40278-9的rpa引物与探针组合、试剂盒及检测方法
CN117095747B (zh) * 2023-08-29 2024-04-30 广东省农业科学院水稻研究所 一种基于线性泛基因组和人工智能模型检测群体倒位或转座子端点基因型的方法
CN117144054B (zh) * 2023-10-27 2024-06-11 莱肯生物科技(海南)有限公司 一种核酸检测方法及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7598361B2 (en) * 1997-11-24 2009-10-06 Monsanto Technology Llc Nucleic acid molecules and other molecules associated with the sucrose pathway
ATE289807T1 (de) * 1998-12-22 2005-03-15 Ca Nat Research Council Transgene pflanzen mit konditional lethalem gen
JP4481656B2 (ja) * 2002-02-27 2010-06-16 ライフ テクノロジーズ コーポレーション ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物
KR101152465B1 (ko) * 2003-05-02 2012-07-04 다우 아그로사이언시즈 엘엘씨 옥수수 이벤트 ｔｃ1507 및 그의 검출 방법
EP1724360A1 (en) * 2005-05-17 2006-11-22 Eppendorf Array Technologies S.A. Identification and/or quantification method of nucleotide sequence(s) elements specific of genetically modified plants on arrays
CA2616535A1 (en) * 2005-07-29 2007-02-08 Monsanto Technology Llc Development of novel germplasm using segregates from transgenic crosses
US7928296B2 (en) * 2006-10-30 2011-04-19 Pioneer Hi-Bred International, Inc. Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof

Also Published As

Publication number Publication date
AR079520A1 (es) 2012-02-01
CN102770553A (zh) 2012-11-07
AU2010330806A1 (en) 2012-07-12
US20110151441A1 (en) 2011-06-23
EP2513326A1 (en) 2012-10-24
WO2011075648A1 (en) 2011-06-23
ZA201204913B (en) 2013-02-27
RU2012130431A (ru) 2014-01-27
KR20120107998A (ko) 2012-10-04
JP2013514780A (ja) 2013-05-02
CO6511270A2 (es) 2012-08-31
BR112012014929A2 (pt) 2015-10-06
MX2012007136A (es) 2012-10-05
CA2783254A1 (en) 2011-06-23
EP2513326A4 (en) 2013-03-27

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