CH643069A5 - Immunochemische bestimmung des lactat dehydrogenase isoenzyms ldh(1). - Google Patents
Immunochemische bestimmung des lactat dehydrogenase isoenzyms ldh(1). Download PDFInfo
- Publication number
- CH643069A5 CH643069A5 CH171379A CH171379A CH643069A5 CH 643069 A5 CH643069 A5 CH 643069A5 CH 171379 A CH171379 A CH 171379A CH 171379 A CH171379 A CH 171379A CH 643069 A5 CH643069 A5 CH 643069A5
- Authority
- CH
- Switzerland
- Prior art keywords
- antibody
- ldhi
- ldh
- activity
- insoluble
- Prior art date
Links
- 108010044467 Isoenzymes Proteins 0.000 title claims description 18
- 230000000984 immunochemical effect Effects 0.000 title description 3
- 102000003855 L-lactate dehydrogenase Human genes 0.000 title 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 title 1
- 238000000034 method Methods 0.000 claims description 25
- 239000002033 PVDF binder Substances 0.000 claims description 14
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 210000002966 serum Anatomy 0.000 claims description 8
- 241000283707 Capra Species 0.000 claims description 3
- 101150095787 ldh2 gene Proteins 0.000 claims description 3
- 101150100271 ldhb gene Proteins 0.000 claims description 3
- 102100031357 L-lactate dehydrogenase C chain Human genes 0.000 claims description 2
- 101150050566 LDHC gene Proteins 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 101150035055 ldh3 gene Proteins 0.000 claims description 2
- 239000007790 solid phase Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 claims 3
- 230000036046 immunoreaction Effects 0.000 claims 2
- 239000010414 supernatant solution Substances 0.000 claims 2
- 239000007795 chemical reaction product Substances 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 208000010125 myocardial infarction Diseases 0.000 description 11
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- BQCIDUSAKPWEOX-UHFFFAOYSA-N 1,1-Difluoroethene Chemical compound FC(F)=C BQCIDUSAKPWEOX-UHFFFAOYSA-N 0.000 description 3
- 229920006370 Kynar Polymers 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 206010061216 Infarction Diseases 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000000747 cardiac effect Effects 0.000 description 2
- 230000007574 infarction Effects 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000283074 Equus asinus Species 0.000 description 1
- 108010088350 Lactate Dehydrogenase 5 Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- SYJRVVFAAIUVDH-UHFFFAOYSA-N ipa isopropanol Chemical compound CC(C)O.CC(C)O SYJRVVFAAIUVDH-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229920002050 silicone resin Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/971—Capture of complex after antigen-antibody reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/882,013 US4224406A (en) | 1978-02-27 | 1978-02-27 | Immunochemical LDH1 assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH643069A5 true CH643069A5 (de) | 1984-05-15 |
Family
ID=25379718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH171379A CH643069A5 (de) | 1978-02-27 | 1979-02-21 | Immunochemische bestimmung des lactat dehydrogenase isoenzyms ldh(1). |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4224406A (OSRAM) |
| JP (1) | JPS54124797A (OSRAM) |
| CA (1) | CA1117414A (OSRAM) |
| CH (1) | CH643069A5 (OSRAM) |
| DE (1) | DE2907228C2 (OSRAM) |
| FR (1) | FR2418464A1 (OSRAM) |
| GB (1) | GB2015157B (OSRAM) |
| IT (1) | IT1166626B (OSRAM) |
| NL (1) | NL185240C (OSRAM) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5009996A (en) * | 1980-06-30 | 1991-04-23 | International Immunoassay Laboratories, Inc. | Two site cross-reaction immunometric sandwich assay method |
| US5009997A (en) * | 1980-06-30 | 1991-04-23 | Shah Vipin D | Two site cross-reaction immunometric sandwich assay method |
| US4387160A (en) * | 1981-02-17 | 1983-06-07 | Hoffmann-La Roche Inc. | Immunochemical assay for creatine kinase-MB isoenzyme |
| CA1185525A (en) * | 1981-06-22 | 1985-04-16 | Hans J. Hager | Immunochemical assay for prostatic acid phosphatase |
| IL78034A (en) * | 1986-03-04 | 1991-08-16 | Univ Ramot | Biosensors comprising antibodies bonded to glassy carbon electrode for immunoassays |
| US6242208B1 (en) | 1988-07-15 | 2001-06-05 | International Reagents Corporation | LDH1 assay |
| JPH0775556B2 (ja) * | 1988-07-15 | 1995-08-16 | 国際試薬株式会社 | Ｌｄｈアイソザイムのｌｄｈ▲下１▼の分別定量法 |
| JPH09304393A (ja) * | 1996-05-15 | 1997-11-28 | Ind Technol Res Inst | 急性心筋梗塞診断キット |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2128670B2 (de) * | 1971-06-09 | 1977-06-30 | Merck Patent Gmbh, 6100 Darmstadt | Immunologische isoenzym-bestimmungsmethode |
| NL7309558A (OSRAM) * | 1972-07-10 | 1974-01-14 | ||
| DE2258822A1 (de) * | 1972-12-01 | 1974-06-06 | Merck Patent Gmbh | Verfahren und mittel zur analyse von isoenzymmustern |
| US3843443A (en) * | 1973-03-30 | 1974-10-22 | J Fishman | Polypeptide materials bound to fluorocarbon polymers |
| DE2350711A1 (de) | 1973-10-06 | 1975-04-17 | Horst Dr Med Spielmann | Verfahren zum herstellen von antikoerpern gegen isoenzyme der laktatdehydrogenase |
| US4046634A (en) * | 1974-05-28 | 1977-09-06 | Mercer Donald W | Assay of isoenzymes by ion exchange chromatography |
| US4048298A (en) * | 1975-02-25 | 1977-09-13 | Rohm And Haas Company | Solid phase double-antibody radioimmunoassay procedure |
-
1978
- 1978-02-27 US US05/882,013 patent/US4224406A/en not_active Expired - Lifetime
-
1979
- 1979-02-08 CA CA000321099A patent/CA1117414A/en not_active Expired
- 1979-02-09 IT IT20097/79A patent/IT1166626B/it active
- 1979-02-21 CH CH171379A patent/CH643069A5/de not_active IP Right Cessation
- 1979-02-22 FR FR7904539A patent/FR2418464A1/fr active Granted
- 1979-02-23 DE DE2907228A patent/DE2907228C2/de not_active Expired
- 1979-02-26 GB GB7906732A patent/GB2015157B/en not_active Expired
- 1979-02-26 JP JP2178979A patent/JPS54124797A/ja active Granted
- 1979-02-27 NL NLAANVRAGE7901537,A patent/NL185240C/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| IT1166626B (it) | 1987-05-05 |
| NL7901537A (nl) | 1979-08-29 |
| FR2418464A1 (fr) | 1979-09-21 |
| GB2015157B (en) | 1982-09-15 |
| JPS6243501B2 (OSRAM) | 1987-09-14 |
| FR2418464B1 (OSRAM) | 1983-10-28 |
| NL185240C (nl) | 1990-02-16 |
| IT7920097A0 (it) | 1979-02-09 |
| CA1117414A (en) | 1982-02-02 |
| JPS54124797A (en) | 1979-09-27 |
| DE2907228A1 (de) | 1979-09-06 |
| DE2907228C2 (de) | 1986-10-09 |
| NL185240B (nl) | 1989-09-18 |
| GB2015157A (en) | 1979-09-05 |
| US4224406A (en) | 1980-09-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUE | Assignment |
Owner name: F. HOFFMANN-LA ROCHE AG |
|
| PL | Patent ceased |