CA3223946A1 - Single-cell profiling of rna translation status - Google Patents
Single-cell profiling of rna translation status Download PDFInfo
- Publication number
- CA3223946A1 CA3223946A1 CA3223946A CA3223946A CA3223946A1 CA 3223946 A1 CA3223946 A1 CA 3223946A1 CA 3223946 A CA3223946 A CA 3223946A CA 3223946 A CA3223946 A CA 3223946A CA 3223946 A1 CA3223946 A1 CA 3223946A1
- Authority
- CA
- Canada
- Prior art keywords
- probe
- complementary
- oligonucleotide
- probes
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/107—Probe or oligonucleotide ligation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163216315P | 2021-06-29 | 2021-06-29 | |
| US63/216,315 | 2021-06-29 | ||
| PCT/US2022/035271 WO2023278409A1 (en) | 2021-06-29 | 2022-06-28 | Single-cell profiling of rna translation status |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3223946A1 true CA3223946A1 (en) | 2023-01-05 |
Family
ID=82742808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3223946A Pending CA3223946A1 (en) | 2021-06-29 | 2022-06-28 | Single-cell profiling of rna translation status |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20240294973A1 (https=) |
| EP (2) | EP4696786A3 (https=) |
| JP (1) | JP2024529290A (https=) |
| KR (1) | KR20240025672A (https=) |
| CN (1) | CN117881797A (https=) |
| AU (1) | AU2022303140A1 (https=) |
| CA (1) | CA3223946A1 (https=) |
| IL (1) | IL309636A (https=) |
| MX (1) | MX2024000117A (https=) |
| WO (1) | WO2023278409A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7502787B2 (ja) | 2018-04-09 | 2024-06-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インサイチュ遺伝子配列決定の方法 |
| WO2022254726A1 (ja) * | 2021-06-04 | 2022-12-08 | 暢 長坂 | 画像解析方法、画像解析装置、及び、画像解析方法を実現するためのコンピュータプログラム |
| EP4733410A2 (en) * | 2022-01-21 | 2026-04-29 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| WO2025059443A1 (en) | 2023-09-15 | 2025-03-20 | The Broad Institute, Inc. | Untargeted multiplexed in situ rna profiling and the uses and means therefor |
| WO2025067452A1 (en) * | 2023-09-27 | 2025-04-03 | Westlake Laboratory Of Life Sciences And Biomedicine | Methods for deep untargeted profiling of spatial transcriptome and proteome in intact tissues |
| WO2025136916A1 (en) | 2023-12-18 | 2025-06-26 | The Board Institute, Inc. | Thick tissue transcriptomics and translatomics |
| WO2025189030A1 (en) | 2024-03-07 | 2025-09-12 | Stellaromics, Inc. | Methods and systems for volumetric imaging |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8486865B2 (en) * | 2008-11-03 | 2013-07-16 | The Regents Of The University Of California | Methods for detecting modification resistant nucleic acids |
| GB201219976D0 (en) * | 2012-11-06 | 2012-12-19 | Nairz Knud | Means and methods for identifying ribosome associated RNA molecules |
| JP7502787B2 (ja) | 2018-04-09 | 2024-06-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インサイチュ遺伝子配列決定の方法 |
-
2022
- 2022-06-28 MX MX2024000117A patent/MX2024000117A/es unknown
- 2022-06-28 CN CN202280059350.8A patent/CN117881797A/zh active Pending
- 2022-06-28 WO PCT/US2022/035271 patent/WO2023278409A1/en not_active Ceased
- 2022-06-28 EP EP25194035.9A patent/EP4696786A3/en active Pending
- 2022-06-28 EP EP22748129.8A patent/EP4363614B1/en active Active
- 2022-06-28 JP JP2024500034A patent/JP2024529290A/ja active Pending
- 2022-06-28 CA CA3223946A patent/CA3223946A1/en active Pending
- 2022-06-28 KR KR1020247003127A patent/KR20240025672A/ko active Pending
- 2022-06-28 AU AU2022303140A patent/AU2022303140A1/en active Pending
- 2022-06-28 IL IL309636A patent/IL309636A/en unknown
- 2022-06-28 US US18/575,255 patent/US20240294973A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4363614A1 (en) | 2024-05-08 |
| MX2024000117A (es) | 2024-03-14 |
| US20240294973A1 (en) | 2024-09-05 |
| KR20240025672A (ko) | 2024-02-27 |
| EP4696786A2 (en) | 2026-02-18 |
| AU2022303140A1 (en) | 2024-01-25 |
| WO2023278409A9 (en) | 2023-03-23 |
| CN117881797A (zh) | 2024-04-12 |
| IL309636A (en) | 2024-02-01 |
| JP2024529290A (ja) | 2024-08-06 |
| EP4696786A3 (en) | 2026-03-11 |
| WO2023278409A1 (en) | 2023-01-05 |
| EP4363614B1 (en) | 2025-08-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| P22 | Classification modified |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P22-P110 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CLASSIFICATION MODIFIED Effective date: 20240806 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250620 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250620 |