JP2024529290A - Ｒｎａの翻訳状態の単一細胞プロファイリング - Google Patents

Ｒｎａの翻訳状態の単一細胞プロファイリング Download PDF

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JP2024529290A
JP2024529290A JP2024500034A JP2024500034A JP2024529290A JP 2024529290 A JP2024529290 A JP 2024529290A JP 2024500034 A JP2024500034 A JP 2024500034A JP 2024500034 A JP2024500034 A JP 2024500034A JP 2024529290 A JP2024529290 A JP 2024529290A
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probe
complementary
oligonucleotide
rna
probes
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JP2024500034A
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Japanese (ja)
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JP2024529290A5 (https=
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ワン，シャオ
ゼン，フー
レン，ジンイー
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Broad Institute Inc
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Broad Institute Inc
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Publication of JP2024529290A5 publication Critical patent/JP2024529290A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
JP2024500034A 2021-06-29 2022-06-28 Ｒｎａの翻訳状態の単一細胞プロファイリング Pending JP2024529290A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163216315P 2021-06-29 2021-06-29
US63/216,315 2021-06-29
PCT/US2022/035271 WO2023278409A1 (en) 2021-06-29 2022-06-28 Single-cell profiling of rna translation status

Publications (2)

Publication Number Publication Date
JP2024529290A true JP2024529290A (ja) 2024-08-06
JP2024529290A5 JP2024529290A5 (https=) 2025-07-08

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ID=82742808

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JP2024500034A Pending JP2024529290A (ja) 2021-06-29 2022-06-28 Ｒｎａの翻訳状態の単一細胞プロファイリング

Country Status (10)

Country Link
US (1) US20240294973A1 (https=)
EP (2) EP4696786A3 (https=)
JP (1) JP2024529290A (https=)
KR (1) KR20240025672A (https=)
CN (1) CN117881797A (https=)
AU (1) AU2022303140A1 (https=)
CA (1) CA3223946A1 (https=)
IL (1) IL309636A (https=)
MX (1) MX2024000117A (https=)
WO (1) WO2023278409A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
WO2022254726A1 (ja) * 2021-06-04 2022-12-08 暢 長坂 画像解析方法、画像解析装置、及び、画像解析方法を実現するためのコンピュータプログラム
EP4733410A2 (en) * 2022-01-21 2026-04-29 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2025059443A1 (en) 2023-09-15 2025-03-20 The Broad Institute, Inc. Untargeted multiplexed in situ rna profiling and the uses and means therefor
WO2025067452A1 (en) * 2023-09-27 2025-04-03 Westlake Laboratory Of Life Sciences And Biomedicine Methods for deep untargeted profiling of spatial transcriptome and proteome in intact tissues
WO2025136916A1 (en) 2023-12-18 2025-06-26 The Board Institute, Inc. Thick tissue transcriptomics and translatomics
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8486865B2 (en) * 2008-11-03 2013-07-16 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
GB201219976D0 (en) * 2012-11-06 2012-12-19 Nairz Knud Means and methods for identifying ribosome associated RNA molecules
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法

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Publication number Publication date
EP4363614A1 (en) 2024-05-08
MX2024000117A (es) 2024-03-14
US20240294973A1 (en) 2024-09-05
KR20240025672A (ko) 2024-02-27
EP4696786A2 (en) 2026-02-18
AU2022303140A1 (en) 2024-01-25
WO2023278409A9 (en) 2023-03-23
CN117881797A (zh) 2024-04-12
IL309636A (en) 2024-02-01
EP4696786A3 (en) 2026-03-11
WO2023278409A1 (en) 2023-01-05
EP4363614B1 (en) 2025-08-06
CA3223946A1 (en) 2023-01-05

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