IL309636A - Determining a profile of RNA translation status in a single cell - Google Patents

Determining a profile of RNA translation status in a single cell

Info

Publication number
IL309636A
IL309636A IL309636A IL30963623A IL309636A IL 309636 A IL309636 A IL 309636A IL 309636 A IL309636 A IL 309636A IL 30963623 A IL30963623 A IL 30963623A IL 309636 A IL309636 A IL 309636A
Authority
IL
Israel
Prior art keywords
rna translation
cell profiling
translation status
status
profiling
Prior art date
Application number
IL309636A
Other languages
English (en)
Hebrew (he)
Inventor
Xiao Wang
Hu Zeng
Jingyi Ren
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Xiao Wang
Hu Zeng
Jingyi Ren
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc, Massachusetts Inst Technology, Xiao Wang, Hu Zeng, Jingyi Ren filed Critical Broad Inst Inc
Publication of IL309636A publication Critical patent/IL309636A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125Rolling circle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107Probe or oligonucleotide ligation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
IL309636A 2021-06-29 2022-06-28 Determining a profile of RNA translation status in a single cell IL309636A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163216315P 2021-06-29 2021-06-29
PCT/US2022/035271 WO2023278409A1 (en) 2021-06-29 2022-06-28 Single-cell profiling of rna translation status

Publications (1)

Publication Number Publication Date
IL309636A true IL309636A (en) 2024-02-01

Family

ID=82742808

Family Applications (1)

Application Number Title Priority Date Filing Date
IL309636A IL309636A (en) 2021-06-29 2022-06-28 Determining a profile of RNA translation status in a single cell

Country Status (10)

Country Link
US (1) US20240294973A1 (https=)
EP (2) EP4696786A3 (https=)
JP (1) JP2024529290A (https=)
KR (1) KR20240025672A (https=)
CN (1) CN117881797A (https=)
AU (1) AU2022303140A1 (https=)
CA (1) CA3223946A1 (https=)
IL (1) IL309636A (https=)
MX (1) MX2024000117A (https=)
WO (1) WO2023278409A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
WO2022254726A1 (ja) * 2021-06-04 2022-12-08 暢 長坂 画像解析方法、画像解析装置、及び、画像解析方法を実現するためのコンピュータプログラム
EP4733410A2 (en) * 2022-01-21 2026-04-29 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2025059443A1 (en) 2023-09-15 2025-03-20 The Broad Institute, Inc. Untargeted multiplexed in situ rna profiling and the uses and means therefor
WO2025067452A1 (en) * 2023-09-27 2025-04-03 Westlake Laboratory Of Life Sciences And Biomedicine Methods for deep untargeted profiling of spatial transcriptome and proteome in intact tissues
WO2025136916A1 (en) 2023-12-18 2025-06-26 The Board Institute, Inc. Thick tissue transcriptomics and translatomics
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8486865B2 (en) * 2008-11-03 2013-07-16 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
GB201219976D0 (en) * 2012-11-06 2012-12-19 Nairz Knud Means and methods for identifying ribosome associated RNA molecules
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法

Also Published As

Publication number Publication date
EP4363614A1 (en) 2024-05-08
MX2024000117A (es) 2024-03-14
US20240294973A1 (en) 2024-09-05
KR20240025672A (ko) 2024-02-27
EP4696786A2 (en) 2026-02-18
AU2022303140A1 (en) 2024-01-25
WO2023278409A9 (en) 2023-03-23
CN117881797A (zh) 2024-04-12
JP2024529290A (ja) 2024-08-06
EP4696786A3 (en) 2026-03-11
WO2023278409A1 (en) 2023-01-05
EP4363614B1 (en) 2025-08-06
CA3223946A1 (en) 2023-01-05

Similar Documents

Publication Publication Date Title
IL309636A (en) Determining a profile of RNA translation status in a single cell
IL304263A (en) RNA structure with self-circulation
EP4090739A4 (en) Single cell sequencing
GB2596787B (en) Culture of organoids
CA211134S (en) Dongle
GB202020061D0 (en) RNA construct
GB202103774D0 (en) Detectiion of Ransomware
GB202205881D0 (en) Methods
GB202020063D0 (en) RNA construct
IL299846A (en) Gene editing to improve joint function
GB202010072D0 (en) Enzyme-mediated process
GB202206507D0 (en) Expression construct
GB2605568B (en) Performing operations using floating point values
GB2605567B (en) Performing operations using floating point values
GB202212749D0 (en) New uses
IL310118A (en) Gene editing to improve joint function
GB202217332D0 (en) Methods
GB202111742D0 (en) Improved process
GB202302887D0 (en) Rna
GB202311825D0 (en) Micro-organism
GB202020064D0 (en) RNA construct
GB202216347D0 (en) Micromanipulator
GB202101005D0 (en) Understanding the prior art of independent touch
GB202213674D0 (en) Vast dongle
CA202776S (en) Dual ended swab tool