CA3211636A1 - Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same - Google Patents

Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same Download PDF

Info

Publication number
CA3211636A1
CA3211636A1 CA3211636A CA3211636A CA3211636A1 CA 3211636 A1 CA3211636 A1 CA 3211636A1 CA 3211636 A CA3211636 A CA 3211636A CA 3211636 A CA3211636 A CA 3211636A CA 3211636 A1 CA3211636 A1 CA 3211636A1
Authority
CA
Canada
Prior art keywords
lysine
variant
lysa
strain
present disclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3211636A
Other languages
English (en)
French (fr)
Inventor
Mi Ryu
Min Woo Moon
In Pyo Hong
Seok Hyun Park
Joon Hyun Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
Original Assignee
Daesang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020210054313A external-priority patent/KR20220126610A/ko
Application filed by Daesang Corp filed Critical Daesang Corp
Publication of CA3211636A1 publication Critical patent/CA3211636A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0102—Diaminopimelate decarboxylase (4.1.1.20)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3211636A 2021-03-09 2021-04-30 Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same Pending CA3211636A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20210030960 2021-03-09
KR10-2021-0030960 2021-03-09
KR1020210054313A KR20220126610A (ko) 2021-03-09 2021-04-27 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR10-2021-0054313 2021-04-27
PCT/KR2021/005509 WO2022191358A1 (ko) 2021-03-09 2021-04-30 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법

Publications (1)

Publication Number Publication Date
CA3211636A1 true CA3211636A1 (en) 2022-09-15

Family

ID=83156207

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3211636A Pending CA3211636A1 (en) 2021-03-09 2021-04-30 Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same

Country Status (11)

Country Link
US (1) US20250283126A1 (enExample)
EP (1) EP4306644A4 (enExample)
JP (1) JP7766704B2 (enExample)
KR (1) KR20230165734A (enExample)
CN (2) CN117778226A (enExample)
AU (1) AU2021432764B2 (enExample)
BR (1) BR112023018219A2 (enExample)
CA (1) CA3211636A1 (enExample)
MX (1) MX2023010588A (enExample)
WO (1) WO2022191358A1 (enExample)
ZA (1) ZA202308733B (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115927432A (zh) * 2022-10-17 2023-04-07 齐鲁工业大学 一种产l-氨基酸的谷氨酸棒状杆菌工程菌的构建方法及应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0775579A (ja) * 1993-09-08 1995-03-20 Mitsubishi Chem Corp ジアミノピメリン酸デカルボキシラーゼをコードする遺伝子を含むｄｎａ断片およびその利用
JP4035855B2 (ja) * 1996-06-05 2008-01-23 味の素株式会社 Ｌ−リジンの製造法
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
WO2008033001A1 (en) * 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100858913B1 (ko) * 2007-03-09 2008-09-17 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
US9169502B2 (en) * 2010-06-15 2015-10-27 Paik Kwang Industrial Co., Ltd. Method of producing L-lysine using a Corynebacterium glutamicum microorganism
KR101492134B1 (ko) * 2012-05-22 2015-02-12 백광산업 주식회사 코리네형 세균 유래 ｓｏｄ 유전자의 프로모터 변이체 및 상기 프로모터 변이체를 이용한 Ｌ-라이신의 생산 방법
EP3387135B1 (en) * 2015-12-07 2021-02-17 Zymergen Inc. Promoters from corynebacterium glutamicum
BR112018011503A2 (pt) * 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
KR20200026881A (ko) 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
CN109750069B (zh) * 2017-11-01 2025-08-01 宁夏伊品生物科技股份有限公司 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
CN110951662B (zh) * 2019-12-26 2024-03-12 新疆梅花氨基酸有限责任公司 一种高产赖氨酸的棒状细菌及其构建方法与应用
KR102269639B1 (ko) * 2020-02-12 2021-06-25 대상 주식회사 L-글루탐산 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-글루탐산의 생산 방법
KR102139806B1 (ko) 2020-02-13 2020-07-30 씨제이제일제당 (주) 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
CN112980867B (zh) * 2021-03-09 2023-04-11 中国科学院深圳先进技术研究院 一种改造谷氨酸棒杆菌启动子的重组菌株及其构建方法与产l-氨基酸的应用

Also Published As

Publication number Publication date
EP4306644A4 (en) 2025-04-16
KR20230165734A (ko) 2023-12-05
AU2021432764B2 (en) 2025-08-14
JP2024511329A (ja) 2024-03-13
US20250283126A1 (en) 2025-09-11
CN115044490B (zh) 2024-03-29
CN117778226A (zh) 2024-03-29
AU2021432764A1 (en) 2023-09-28
MX2023010588A (es) 2023-12-08
JP7766704B2 (ja) 2025-11-10
BR112023018219A2 (pt) 2023-11-28
CN115044490A (zh) 2022-09-13
ZA202308733B (en) 2025-06-25
EP4306644A1 (en) 2024-01-17
WO2022191358A1 (ko) 2022-09-15

Similar Documents

Publication Publication Date Title
JP2025168674A (ja) Ｌ－リジン生産能が向上したコリネバクテリウム・グルタミカム変異株およびこれを用いたｌ－リジンの生産方法
EP4306644A1 (en) Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same
US20250027125A1 (en) Corynebacterium glutamicum variant having improved l-lysine production ability and method for producing l-lysine by using same
EP3964572A1 (en) Mutant of corynebacterium glutamicum with enhanced l-lysine productivity and method for preparing l-lysine using the same
AU2021442398B2 (en) Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine using same
CN115261246A (zh) L-赖氨酸生产能力得到提高的谷氨酸棒状杆菌突变株及利用其的l-赖氨酸的生产方法
RU2834919C1 (ru) Вариант Corynebacterium glutamicum, обладающий улучшенной способностью продуцировать L-лизин, и способ продуцирования L-лизина с его использованием
AU2021443607B2 (en) Corynebacterium glutamicum variant with improved l-lysine production ability, and method for producing l-lysine using same
AU2021442655B2 (en) Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same
KR102685495B1 (ko) L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
EP4417696A1 (en) Corynebacterium glutamicum variant having improved l-lysine production ability and method for producing l-lysine by using same
KR20220126610A (ko) L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
JP2024014656A (ja) Ｌ－アルギニンまたはｌ－シトルリン生産能が向上したコリネバクテリウム属微生物およびこれを用いたｌ－アルギニンまたはｌ－シトルリンの生産方法

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20240314

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20241016

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20250213

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250219

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 4TH ANNIV.) - STANDARD

Year of fee payment: 4

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250228

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250228

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250228

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250515

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250515