AU2021432764B2 - Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same - Google Patents

Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same

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Publication number
AU2021432764B2
AU2021432764B2 AU2021432764A AU2021432764A AU2021432764B2 AU 2021432764 B2 AU2021432764 B2 AU 2021432764B2 AU 2021432764 A AU2021432764 A AU 2021432764A AU 2021432764 A AU2021432764 A AU 2021432764A AU 2021432764 B2 AU2021432764 B2 AU 2021432764B2
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AU
Australia
Prior art keywords
lysa
artificial sequence
dna
lysine
variant
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Active
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AU2021432764A
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English (en)
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AU2021432764A1 (en
Inventor
In Pyo Hong
Min Woo Moon
Joon Hyun Park
Seok Hyun Park
Mi Ryu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
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CJ CheilJedang Corp
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Priority claimed from KR1020210054313A external-priority patent/KR20220126610A/ko
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Publication of AU2021432764A1 publication Critical patent/AU2021432764A1/en
Assigned to CJ CHEILJEDANG CORPORATION reassignment CJ CHEILJEDANG CORPORATION Request for Assignment Assignors: DAESANG CORPORATION
Application granted granted Critical
Publication of AU2021432764B2 publication Critical patent/AU2021432764B2/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0102—Diaminopimelate decarboxylase (4.1.1.20)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2021432764A 2021-03-09 2021-04-30 Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same Active AU2021432764B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20210030960 2021-03-09
KR10-2021-0030960 2021-03-09
KR1020210054313A KR20220126610A (ko) 2021-03-09 2021-04-27 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR10-2021-0054313 2021-04-27
PCT/KR2021/005509 WO2022191358A1 (ko) 2021-03-09 2021-04-30 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법

Publications (2)

Publication Number Publication Date
AU2021432764A1 AU2021432764A1 (en) 2023-09-28
AU2021432764B2 true AU2021432764B2 (en) 2025-08-14

Family

ID=83156207

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021432764A Active AU2021432764B2 (en) 2021-03-09 2021-04-30 Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same

Country Status (11)

Country Link
US (1) US20250283126A1 (enExample)
EP (1) EP4306644A4 (enExample)
JP (1) JP7766704B2 (enExample)
KR (1) KR20230165734A (enExample)
CN (2) CN117778226A (enExample)
AU (1) AU2021432764B2 (enExample)
BR (1) BR112023018219A2 (enExample)
CA (1) CA3211636A1 (enExample)
MX (1) MX2023010588A (enExample)
WO (1) WO2022191358A1 (enExample)
ZA (1) ZA202308733B (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115927432A (zh) * 2022-10-17 2023-04-07 齐鲁工业大学 一种产l-氨基酸的谷氨酸棒状杆菌工程菌的构建方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001049854A2 (en) * 1999-12-30 2001-07-12 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria

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JPH0775579A (ja) * 1993-09-08 1995-03-20 Mitsubishi Chem Corp ジアミノピメリン酸デカルボキシラーゼをコードする遺伝子を含むｄｎａ断片およびその利用
JP4035855B2 (ja) * 1996-06-05 2008-01-23 味の素株式会社 Ｌ−リジンの製造法
WO2008033001A1 (en) * 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100858913B1 (ko) * 2007-03-09 2008-09-17 한국과학기술원 부위특이적 변이에 의해 제조된 고수율의 ｌ－쓰레오닌생산균주 및 이를 이용한 ｌ－쓰레오닌의 제조방법
US9169502B2 (en) * 2010-06-15 2015-10-27 Paik Kwang Industrial Co., Ltd. Method of producing L-lysine using a Corynebacterium glutamicum microorganism
KR101492134B1 (ko) * 2012-05-22 2015-02-12 백광산업 주식회사 코리네형 세균 유래 ｓｏｄ 유전자의 프로모터 변이체 및 상기 프로모터 변이체를 이용한 Ｌ-라이신의 생산 방법
EP3387135B1 (en) * 2015-12-07 2021-02-17 Zymergen Inc. Promoters from corynebacterium glutamicum
BR112018011503A2 (pt) * 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
KR20200026881A (ko) 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
CN109750069B (zh) * 2017-11-01 2025-08-01 宁夏伊品生物科技股份有限公司 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
CN110951662B (zh) * 2019-12-26 2024-03-12 新疆梅花氨基酸有限责任公司 一种高产赖氨酸的棒状细菌及其构建方法与应用
KR102269639B1 (ko) * 2020-02-12 2021-06-25 대상 주식회사 L-글루탐산 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-글루탐산의 생산 방법
KR102139806B1 (ko) 2020-02-13 2020-07-30 씨제이제일제당 (주) 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
CN112980867B (zh) * 2021-03-09 2023-04-11 中国科学院深圳先进技术研究院 一种改造谷氨酸棒杆菌启动子的重组菌株及其构建方法与产l-氨基酸的应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001049854A2 (en) * 1999-12-30 2001-07-12 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria

Also Published As

Publication number Publication date
EP4306644A4 (en) 2025-04-16
KR20230165734A (ko) 2023-12-05
CA3211636A1 (en) 2022-09-15
JP2024511329A (ja) 2024-03-13
US20250283126A1 (en) 2025-09-11
CN115044490B (zh) 2024-03-29
CN117778226A (zh) 2024-03-29
AU2021432764A1 (en) 2023-09-28
MX2023010588A (es) 2023-12-08
JP7766704B2 (ja) 2025-11-10
BR112023018219A2 (pt) 2023-11-28
CN115044490A (zh) 2022-09-13
ZA202308733B (en) 2025-06-25
EP4306644A1 (en) 2024-01-17
WO2022191358A1 (ko) 2022-09-15

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Owner name: CJ CHEILJEDANG CORPORATION

Free format text: FORMER APPLICANT(S): DAESANG CORPORATION

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