CA3131115A1 - Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing - Google Patents

Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing Download PDF

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Publication number
CA3131115A1
CA3131115A1 CA3131115A CA3131115A CA3131115A1 CA 3131115 A1 CA3131115 A1 CA 3131115A1 CA 3131115 A CA3131115 A CA 3131115A CA 3131115 A CA3131115 A CA 3131115A CA 3131115 A1 CA3131115 A1 CA 3131115A1
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CA
Canada
Prior art keywords
oligonucleotide
sequence
dna
strand
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3131115A
Other languages
English (en)
French (fr)
Inventor
Lacey MERRILL
Marc PRINDLE
Samantha VELLUCCI
Jagadeeswaran CHANDRASEKAR
Mark Stamatios Kokoris
Gerson AGUIRRE
John Tabone
Robert N. Mcruer
Michael Lee
Matthew CORNING
Greg THIESSEN
Salka KELLER BARRETT
Christian BERRIOS
Aaron Jacobs
Taylor LEHMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Stratos Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratos Genomics Inc filed Critical Stratos Genomics Inc
Publication of CA3131115A1 publication Critical patent/CA3131115A1/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08FMACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F255/00Macromolecular compounds obtained by polymerising monomers on to polymers of hydrocarbons as defined in group C08F10/00
    • C08F255/02Macromolecular compounds obtained by polymerising monomers on to polymers of hydrocarbons as defined in group C08F10/00 on to polymers of olefins having two or three carbon atoms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3131115A 2019-02-21 2020-02-20 Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing Pending CA3131115A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962808768P 2019-02-21 2019-02-21
US62/808,768 2019-02-21
US201962826805P 2019-03-29 2019-03-29
US62/826,805 2019-03-29
PCT/US2020/019131 WO2020172479A1 (en) 2019-02-21 2020-02-20 Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing

Publications (1)

Publication Number Publication Date
CA3131115A1 true CA3131115A1 (en) 2020-08-27

Family

ID=72144173

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3131115A Pending CA3131115A1 (en) 2019-02-21 2020-02-20 Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing

Country Status (7)

Country Link
US (1) US20220042075A1 (de)
EP (1) EP3927869A4 (de)
JP (2) JP2022523362A (de)
CN (1) CN113631764A (de)
AU (1) AU2020224663B2 (de)
CA (1) CA3131115A1 (de)
WO (1) WO2020172479A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020236526A1 (en) * 2019-05-23 2020-11-26 Stratos Genomics, Inc. Translocation control elements, reporter codes, and further means for translocation control for use in nanopore sequencing
WO2024083982A1 (en) 2022-10-21 2024-04-25 F. Hoffmann-La Roche Ag Detection of modified nucleobases in nucleic acid samples

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032790A1 (en) * 1997-01-27 1998-07-30 Flowgenix Corporation Porous articles with surface functionality and uses thereof
EP2436778A3 (de) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photospaltbare fluoreszierende Nukleotide zur DNA-Sequenzierung auf einem durch ortsspezifische Kopplungschemie konstruierten Chip
DE602005012319D1 (de) * 2004-11-22 2009-02-26 Peter Birk Rasmussen Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
ES2559313T3 (es) * 2007-06-19 2016-02-11 Stratos Genomics Inc. Secuenciación de ácidos nucleicos de alto rendimiento por expansión
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
CN103502448B (zh) * 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US9670526B2 (en) * 2012-11-09 2017-06-06 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
KR20150135353A (ko) * 2013-03-14 2015-12-02 엔브이에스 테크놀로지스 인코포레이티드 생체 분자를 격리시키기 위한 표면 산화 및 관련 방법
AU2014315287A1 (en) * 2013-09-03 2015-03-12 Moderna Therapeutics, Inc. Chimeric polynucleotides
JP2017537657A (ja) * 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
SG11202102029TA (en) * 2018-08-28 2021-03-30 10X Genomics Inc Methods for generating spatially barcoded arrays

Also Published As

Publication number Publication date
EP3927869A1 (de) 2021-12-29
EP3927869A4 (de) 2023-04-26
WO2020172479A1 (en) 2020-08-27
AU2020224663A1 (en) 2021-07-22
JP2024026147A (ja) 2024-02-28
AU2020224663B2 (en) 2022-12-08
JP2022523362A (ja) 2022-04-22
CN113631764A (zh) 2021-11-09
US20220042075A1 (en) 2022-02-10

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