CA3081687A1 - Dna plasmids for the fast generation of homologous recombination vectors for cell line development - Google Patents

Dna plasmids for the fast generation of homologous recombination vectors for cell line development Download PDF

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Publication number
CA3081687A1
CA3081687A1 CA3081687A CA3081687A CA3081687A1 CA 3081687 A1 CA3081687 A1 CA 3081687A1 CA 3081687 A CA3081687 A CA 3081687A CA 3081687 A CA3081687 A CA 3081687A CA 3081687 A1 CA3081687 A1 CA 3081687A1
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cell
sequence
tag
gene
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CA3081687A
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English (en)
French (fr)
Inventor
Oscar M. Perez-Leal
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Temple Univ School of Medicine
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Temple Univ School of Medicine
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3081687A 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development Pending CA3081687A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

Publications (1)

Publication Number Publication Date
CA3081687A1 true CA3081687A1 (en) 2018-08-09

Family

ID=63040011

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3081687A Pending CA3081687A1 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

Country Status (8)

Country Link
US (2) US12359222B2 (cg-RX-API-DMAC7.html)
EP (2) EP3535394B1 (cg-RX-API-DMAC7.html)
JP (1) JP7418796B2 (cg-RX-API-DMAC7.html)
KR (2) KR102736524B1 (cg-RX-API-DMAC7.html)
CN (1) CN110199020B (cg-RX-API-DMAC7.html)
CA (1) CA3081687A1 (cg-RX-API-DMAC7.html)
IL (1) IL266422A (cg-RX-API-DMAC7.html)
WO (1) WO2018144087A2 (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102736524B1 (ko) 2016-11-03 2024-11-29 템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
WO2020141109A1 (en) * 2018-12-30 2020-07-09 F. Hoffmann-La Roche Ag Method for the selection of cells based on crispr/cas-controlled integration of a detectable tag to a target protein
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination

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US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
IL102404A0 (en) 1992-07-03 1993-01-14 Daniel Zurr Method and means for the production of gene products
DE69433034T3 (de) 1993-04-21 2010-04-15 The University Court Of The University Of Edinburgh Expression von heterologen genen nach einem ziel-expressionsprofil
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
EP0784630B1 (en) 1994-10-11 2004-09-15 University Of California Selective inhibition of internally initiated rna translation
CA2304173A1 (en) * 1997-09-23 1999-04-01 Genvec, Inc. Plasmids for construction of eukaryotic viral vectors
NZ525134A (en) 1999-03-02 2004-09-24 Invitrogen Corp Compositions and methods for use in recombinational cloning of nucleic acids
JP5442177B2 (ja) * 2001-02-23 2014-03-12 ユニベルスィテ リーブル ドゥ ブリュッセル 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法
JPWO2003020940A1 (ja) * 2001-08-28 2004-12-16 日本たばこ産業株式会社 高等植物のゲノム改変法
JP2007509923A (ja) * 2003-10-31 2007-04-19 ファイザー・プロダクツ・インク 肥満関連状態の処置としてのホスホジエステラーゼ９阻害
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Also Published As

Publication number Publication date
KR102736524B1 (ko) 2024-11-29
JP2019537445A (ja) 2019-12-26
KR102590861B1 (ko) 2023-10-18
EP3535394A4 (en) 2020-05-27
US20190284582A1 (en) 2019-09-19
EP4219709A1 (en) 2023-08-02
WO2018144087A3 (en) 2018-10-25
KR20190113759A (ko) 2019-10-08
IL266422A (en) 2019-06-30
US12359222B2 (en) 2025-07-15
EP3535394B1 (en) 2023-04-12
CN110199020B (zh) 2024-06-18
JP7418796B2 (ja) 2024-01-22
EP3535394A2 (en) 2019-09-11
CN110199020A (zh) 2019-09-03
US10883120B2 (en) 2021-01-05
US20200002729A1 (en) 2020-01-02
KR20230148274A (ko) 2023-10-24
WO2018144087A2 (en) 2018-08-09

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