IL266422A - DNA plasmids for rapid production of homologous recombination vectors for cell line development - Google Patents
DNA plasmids for rapid production of homologous recombination vectors for cell line developmentInfo
- Publication number
- IL266422A IL266422A IL266422A IL26642219A IL266422A IL 266422 A IL266422 A IL 266422A IL 266422 A IL266422 A IL 266422A IL 26642219 A IL26642219 A IL 26642219A IL 266422 A IL266422 A IL 266422A
- Authority
- IL
- Israel
- Prior art keywords
- cell line
- homologous recombination
- dna plasmids
- recombination vectors
- line development
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Veterinary Medicine (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662416802P | 2016-11-03 | 2016-11-03 | |
| PCT/US2017/059816 WO2018144087A2 (en) | 2016-11-03 | 2017-11-03 | Dna plasmids for the fast generation of homologous recombination vectors for cell line development |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL266422A true IL266422A (en) | 2019-06-30 |
Family
ID=63040011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL266422A IL266422A (en) | 2016-11-03 | 2019-05-02 | DNA plasmids for rapid production of homologous recombination vectors for cell line development |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US12359222B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3535394B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP7418796B2 (cg-RX-API-DMAC7.html) |
| KR (2) | KR102736524B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN110199020B (cg-RX-API-DMAC7.html) |
| CA (1) | CA3081687A1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL266422A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2018144087A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102736524B1 (ko) | 2016-11-03 | 2024-11-29 | 템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 | 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 |
| CN109402172B (zh) * | 2018-11-23 | 2021-11-26 | 四川农业大学 | 一种鸭坦布苏报告病毒的制备方法及其产品和应用 |
| WO2020141109A1 (en) * | 2018-12-30 | 2020-07-09 | F. Hoffmann-La Roche Ag | Method for the selection of cells based on crispr/cas-controlled integration of a detectable tag to a target protein |
| CN111235183B (zh) * | 2020-01-13 | 2021-07-27 | 东南大学 | 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用 |
| CN116721700B (zh) * | 2023-08-08 | 2024-01-12 | 中国人民解放军军事科学院军事医学研究院 | 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用 |
| WO2025106834A1 (en) * | 2023-11-17 | 2025-05-22 | Temple University-Of The Commonwealth System Of Higher Education | A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4937190A (en) | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
| US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
| IL102404A0 (en) | 1992-07-03 | 1993-01-14 | Daniel Zurr | Method and means for the production of gene products |
| DE69433034T3 (de) | 1993-04-21 | 2010-04-15 | The University Court Of The University Of Edinburgh | Expression von heterologen genen nach einem ziel-expressionsprofil |
| FR2722208B1 (fr) | 1994-07-05 | 1996-10-04 | Inst Nat Sante Rech Med | Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique |
| EP0784630B1 (en) | 1994-10-11 | 2004-09-15 | University Of California | Selective inhibition of internally initiated rna translation |
| CA2304173A1 (en) * | 1997-09-23 | 1999-04-01 | Genvec, Inc. | Plasmids for construction of eukaryotic viral vectors |
| NZ525134A (en) | 1999-03-02 | 2004-09-24 | Invitrogen Corp | Compositions and methods for use in recombinational cloning of nucleic acids |
| JP5442177B2 (ja) * | 2001-02-23 | 2014-03-12 | ユニベルスィテ リーブル ドゥ ブリュッセル | 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法 |
| JPWO2003020940A1 (ja) * | 2001-08-28 | 2004-12-16 | 日本たばこ産業株式会社 | 高等植物のゲノム改変法 |
| JP2007509923A (ja) * | 2003-10-31 | 2007-04-19 | ファイザー・プロダクツ・インク | 肥満関連状態の処置としてのホスホジエステラーゼ９阻害 |
| JP2007124915A (ja) | 2005-11-01 | 2007-05-24 | Tokai Univ | リコンビニアリングコンストラクト、及びジーンターゲティングコンストラクト作製用ベクター |
| JP2008253254A (ja) * | 2007-03-14 | 2008-10-23 | National Institutes Of Natural Sciences | 高効率変異導入法 |
| US20090111099A1 (en) | 2007-10-27 | 2009-04-30 | Yongsheng Ma | Promoter Detection and Analysis |
| US10722562B2 (en) * | 2008-07-23 | 2020-07-28 | Immudex Aps | Combinatorial analysis and repair |
| AU2009279949A1 (en) | 2008-08-04 | 2010-02-11 | Merck Sharp & Dohme Corp. | Urea derivatives as antibacterial agents |
| WO2011101696A1 (en) * | 2010-02-18 | 2011-08-25 | Cellectis | Improved meganuclease recombination system |
| WO2012116274A2 (en) | 2011-02-25 | 2012-08-30 | Recombinetics, Inc. | Genetically modified animals and methods for making the same |
| WO2015017866A1 (en) * | 2013-08-02 | 2015-02-05 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
| JP6374176B2 (ja) * | 2014-02-13 | 2018-08-15 | 学校法人 名城大学 | 植物体に用いる組換えベクター及びその利用 |
| EP3078675A1 (en) * | 2015-04-10 | 2016-10-12 | Ares Trading S.A. | Induction dosing regimen for the treatment of tnf alpha mediated disorders |
| KR102736524B1 (ko) | 2016-11-03 | 2024-11-29 | 템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 | 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 |
-
2017
- 2017-11-03 KR KR1020237035059A patent/KR102736524B1/ko active Active
- 2017-11-03 CA CA3081687A patent/CA3081687A1/en active Pending
- 2017-11-03 US US16/346,614 patent/US12359222B2/en active Active
- 2017-11-03 EP EP17895091.1A patent/EP3535394B1/en active Active
- 2017-11-03 EP EP23167143.9A patent/EP4219709A1/en active Pending
- 2017-11-03 JP JP2019523781A patent/JP7418796B2/ja active Active
- 2017-11-03 KR KR1020197015851A patent/KR102590861B1/ko active Active
- 2017-11-03 CN CN201780082232.8A patent/CN110199020B/zh active Active
- 2017-11-03 WO PCT/US2017/059816 patent/WO2018144087A2/en not_active Ceased
-
2019
- 2019-05-02 IL IL266422A patent/IL266422A/en unknown
- 2019-09-12 US US16/568,771 patent/US10883120B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR102736524B1 (ko) | 2024-11-29 |
| JP2019537445A (ja) | 2019-12-26 |
| KR102590861B1 (ko) | 2023-10-18 |
| EP3535394A4 (en) | 2020-05-27 |
| US20190284582A1 (en) | 2019-09-19 |
| EP4219709A1 (en) | 2023-08-02 |
| WO2018144087A3 (en) | 2018-10-25 |
| KR20190113759A (ko) | 2019-10-08 |
| US12359222B2 (en) | 2025-07-15 |
| EP3535394B1 (en) | 2023-04-12 |
| CN110199020B (zh) | 2024-06-18 |
| JP7418796B2 (ja) | 2024-01-22 |
| EP3535394A2 (en) | 2019-09-11 |
| CN110199020A (zh) | 2019-09-03 |
| US10883120B2 (en) | 2021-01-05 |
| US20200002729A1 (en) | 2020-01-02 |
| KR20230148274A (ko) | 2023-10-24 |
| CA3081687A1 (en) | 2018-08-09 |
| WO2018144087A2 (en) | 2018-08-09 |
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