CA3079439C - Methods for differentiating mesenchymal stem cells - Google Patents

Methods for differentiating mesenchymal stem cells Download PDF

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Publication number
CA3079439C
CA3079439C CA3079439A CA3079439A CA3079439C CA 3079439 C CA3079439 C CA 3079439C CA 3079439 A CA3079439 A CA 3079439A CA 3079439 A CA3079439 A CA 3079439A CA 3079439 C CA3079439 C CA 3079439C
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Canada
Prior art keywords
msc
cells
derived cells
heparin
bone
Prior art date
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Active
Application number
CA3079439A
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English (en)
French (fr)
Other versions
CA3079439A1 (en
Inventor
Sandra PIETRI
Xuan Mai NGUYEN
Enrico Bastianelli
Sabrina ENA
Pierre-Yves LARUELLE
Isabelle Tytgat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bone Therapeutics SA
Original Assignee
Ena Sabrina
Laruelle Pierre Yves
Pietri Sandra
Bone Therapeutics SA
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Application filed by Ena Sabrina, Laruelle Pierre Yves, Pietri Sandra, Bone Therapeutics SA filed Critical Ena Sabrina
Publication of CA3079439A1 publication Critical patent/CA3079439A1/en
Application granted granted Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CA3079439A 2017-10-20 2018-09-25 Methods for differentiating mesenchymal stem cells Active CA3079439C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17197605.3 2017-10-20
EP17197605 2017-10-20
PCT/EP2018/076030 WO2019076591A1 (en) 2017-10-20 2018-09-25 METHODS FOR DIFFERENCING MESENCHYMAL STEM CELLS

Publications (2)

Publication Number Publication Date
CA3079439A1 CA3079439A1 (en) 2019-04-25
CA3079439C true CA3079439C (en) 2022-08-09

Family

ID=60153205

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3079439A Active CA3079439C (en) 2017-10-20 2018-09-25 Methods for differentiating mesenchymal stem cells

Country Status (13)

Country Link
US (2) US11447749B2 (https=)
EP (1) EP3697894A1 (https=)
JP (1) JP7039711B2 (https=)
KR (1) KR102185697B1 (https=)
CN (1) CN111247240A (https=)
AU (1) AU2018351804B2 (https=)
BE (1) BE1025935A9 (https=)
BR (1) BR112020007775A2 (https=)
CA (1) CA3079439C (https=)
IL (1) IL273987B (https=)
RU (1) RU2020113411A (https=)
SG (1) SG11202003524XA (https=)
WO (1) WO2019076591A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1026595B9 (fr) * 2018-09-25 2020-11-03 Bone Therapeutics Sa Procedes de differenciation de cellules souches mesenchymateuses
CN113648417A (zh) * 2021-03-15 2021-11-16 中国人民解放军军事科学院军事医学研究院 Postn作为调控间充质干细胞分化能力的标志物及其应用
CN113355279A (zh) * 2021-06-07 2021-09-07 深圳市中佳生物医疗科技有限公司 一种人韧带成纤维细胞的制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4764369A (en) 1983-07-14 1988-08-16 New York Blood Center Inc. Undenatured virus-free biologically active protein derivatives
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5811094A (en) 1990-11-16 1998-09-22 Osiris Therapeutics, Inc. Connective tissue regeneration using human mesenchymal stem cell preparations
US5837539A (en) 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
US5972703A (en) 1994-08-12 1999-10-26 The Regents Of The University Of Michigan Bone precursor cells: compositions and methods
US5736396A (en) 1995-01-24 1998-04-07 Case Western Reserve University Lineage-directed induction of human mesenchymal stem cell differentiation
US5827740A (en) 1996-07-30 1998-10-27 Osiris Therapeutics, Inc. Adipogenic differentiation of human mesenchymal stem cells
AU2003202988A1 (en) 2002-01-14 2003-07-30 The Board Of Trustees Of The University Of Illinois Use of modified pyrimidine compounds to promote stem cell migration and proliferation
CA2640794C (en) 2006-02-16 2015-04-14 Universite Libre De Bruxelles A method for osteogenic differentiation of bone marrow stem cells (bmsc) and uses thereof
WO2008129563A2 (en) * 2007-04-23 2008-10-30 Stempeutics Research Private Limited, Human mesenchymal stem cells and preparation thereof
US20100278788A1 (en) 2008-01-11 2010-11-04 Bone Therapeutics, S.A. Osteogenic Differentiation Of Bone Marrow Stem Cells And Mesenchymal Stem Cells Using A Combination Of Growth Factors
HK1202581A1 (en) 2012-02-13 2015-10-02 Gamida-Cell Ltd. Mesenchymal stem cells conditioned medium and methods of generating and using the same
EP2943206A4 (en) * 2013-01-12 2017-01-04 Cesca Therapeutics Inc. Rapid infusion of autologous bone marrow derived stem cells
EP3074505B1 (en) 2014-02-14 2018-06-06 National University of Ireland Galway Serum-free medium
JP6446222B2 (ja) * 2014-09-30 2018-12-26 株式会社ジーシー 軟骨分化培養液、及び軟骨組織の製造方法

Also Published As

Publication number Publication date
BE1025935B9 (fr) 2019-10-29
JP7039711B2 (ja) 2022-03-22
AU2018351804B2 (en) 2020-07-09
JP2020537539A (ja) 2020-12-24
US20230042822A1 (en) 2023-02-09
BE1025935A1 (fr) 2019-08-13
SG11202003524XA (en) 2020-05-28
BE1025935A9 (fr) 2019-11-05
CN111247240A (zh) 2020-06-05
CA3079439A1 (en) 2019-04-25
EP3697894A1 (en) 2020-08-26
US11447749B2 (en) 2022-09-20
US20200354682A1 (en) 2020-11-12
IL273987B (en) 2021-06-30
AU2018351804A1 (en) 2020-05-14
BE1025935B1 (fr) 2019-10-18
KR102185697B1 (ko) 2020-12-03
RU2020113411A3 (https=) 2021-11-22
IL273987A (en) 2020-05-31
KR20200063241A (ko) 2020-06-04
WO2019076591A1 (en) 2019-04-25
RU2020113411A (ru) 2021-11-22
BR112020007775A2 (pt) 2020-10-20

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