CA3052635A1 - Engineered biosynthetic pathways for production of tyramine by fermentation - Google Patents
Engineered biosynthetic pathways for production of tyramine by fermentation Download PDFInfo
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- CA3052635A1 CA3052635A1 CA3052635A CA3052635A CA3052635A1 CA 3052635 A1 CA3052635 A1 CA 3052635A1 CA 3052635 A CA3052635 A CA 3052635A CA 3052635 A CA3052635 A CA 3052635A CA 3052635 A1 CA3052635 A1 CA 3052635A1
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- C12N9/90—Isomerases (5.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
- C12Y103/01012—Prephenate dehydrogenase (1.3.1.12)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01002—Transaldolase (2.2.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01054—3-Deoxy-7-phosphoheptulonate synthase (2.5.1.54)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01071—Shikimate kinase (2.7.1.71)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01025—Tyrosine decarboxylase (4.1.1.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y504/00—Intramolecular transferases (5.4)
- C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
- C12Y504/99005—Chorismate mutase (5.4.99.5)
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- Biophysics (AREA)
- Plant Pathology (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| US201762455428P | 2017-02-06 | 2017-02-06 | |
| US62/455,428 | 2017-02-06 | ||
| PCT/US2018/017127 WO2018203947A2 (en) | 2017-02-06 | 2018-02-06 | Engineered biosynthetic pathways for production of tyramine by fermentation |
Publications (1)
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|---|---|
| CA3052635A1 true CA3052635A1 (en) | 2018-11-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3052635A Pending CA3052635A1 (en) | 2017-02-06 | 2018-02-06 | Engineered biosynthetic pathways for production of tyramine by fermentation |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11028416B2 (https=) |
| EP (1) | EP3577220A2 (https=) |
| JP (1) | JP2020513790A (https=) |
| KR (1) | KR20190120238A (https=) |
| CN (1) | CN110494560A (https=) |
| CA (1) | CA3052635A1 (https=) |
| WO (1) | WO2018203947A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2850077C1 (ru) * | 2025-04-04 | 2025-11-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Горский государственный аграрный университет" | Штамм лактобактерий Enterococcus faecium 22-2024 ВКПМ В-14767 - продуцент молочной кислоты |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112410379B (zh) | 2013-11-04 | 2024-12-31 | 小利兰·斯坦福大学托管委员会 | 产生苄基异喹啉生物碱(bia)前体的微生物以及制备和使用所述前体的方法 |
| WO2016183023A1 (en) | 2015-05-08 | 2016-11-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of producing epimerases and benzylisoquinoline alkaloids |
| WO2018203947A2 (en) | 2017-02-06 | 2018-11-08 | Zymergen Inc. | Engineered biosynthetic pathways for production of tyramine by fermentation |
| CA3087269A1 (en) | 2018-01-10 | 2019-07-18 | Brightseed, Inc. | Method for modulating metabolism |
| EP3656857A1 (en) * | 2018-11-21 | 2020-05-27 | Danmarks Tekniske Universitet | Utilization of phosphoketolase for production of aromatic amino acid derived products |
| CN113784758B (zh) | 2019-03-08 | 2025-03-07 | 小利兰·斯坦福大学托管委员会 | 产生莨菪烷生物碱(ta)的非植物宿主细胞及其制备和使用方法 |
| WO2020232027A1 (en) * | 2019-05-13 | 2020-11-19 | Zymergen Inc. | Stabilization of amines with sulfur compounds |
| CA3158769A1 (en) * | 2019-10-25 | 2021-04-29 | Brightseed, Inc. | Recombinant cell, extract, consumable product and method for production of bioactive plant metabolite |
| CN116368233A (zh) * | 2020-08-20 | 2023-06-30 | 齐默尔根公司 | 通过发酵生产4-氨基苯乙胺的工程化生物合成途径 |
| CN113174375B (zh) * | 2021-04-23 | 2022-11-04 | 天津大学 | Aro3蛋白突变体及其应用 |
| WO2022245340A1 (en) * | 2021-05-18 | 2022-11-24 | Zymergen Inc. | Engineered biosynthetic pathways for production of deoxyhydrochorismic acid by fermentation |
| CN113684165B (zh) * | 2021-08-12 | 2023-07-25 | 江南大学 | 一种重组谷氨酸棒杆菌及其在生产l-谷氨酰胺中的应用 |
| EP4198123A1 (de) * | 2021-12-17 | 2023-06-21 | Covestro Deutschland AG | Besonders geeignete 3-desoxyarabinoheptulosanat-7-phosphat-synthase zur fermentativen herstellung von ortho-aminobenzoesäure |
| CN116083387B (zh) * | 2022-12-29 | 2025-09-02 | 华熙生物科技股份有限公司 | 酶、生产红景天苷的菌株及生产方法 |
| CN116042553B (zh) * | 2022-12-29 | 2025-08-26 | 浙江工业大学 | 甘油醛-3-磷酸脱氢酶突变体及在提高o-琥珀酰-l-高丝氨酸产量中的应用 |
| CN117736960B (zh) * | 2024-02-21 | 2024-05-07 | 滨州医学院 | 一种小白链霉菌基因工程菌及其应用 |
| CN119144595B (zh) * | 2024-11-13 | 2025-02-14 | 中国科学院微生物研究所 | 一种酪氨酸脱羧酶突变体及其在发酵合成3-氨基-1-丙醇中的应用 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3128411A1 (de) | 1980-07-18 | 1983-09-15 | Unisearch Ltd., 2033 Kensington, New South Wales | Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin |
| EP0077196A3 (en) | 1981-10-09 | 1984-06-06 | Genex Corporation | Aromatic amino acid-producing microorganisms |
| JPH07108228B2 (ja) | 1990-10-15 | 1995-11-22 | 味の素株式会社 | 温度感受性プラスミド |
| DK0782621T3 (da) | 1994-09-16 | 2006-07-03 | Texas A & M Univ Sys | Mikroorganismer samt fremgangsmåde til overproduktion af DAHP ved hjælp af klonet PPS-gen |
| US6210937B1 (en) | 1997-04-22 | 2001-04-03 | Bechtel Bwxt Idaho, Llc | Development of genetically engineered bacteria for production of selected aromatic compounds |
| US6297055B1 (en) | 1998-09-08 | 2001-10-02 | E. I. Du Pont De Nemours And Company | Amino acid decarboxylases |
| US6600077B1 (en) | 1999-01-29 | 2003-07-29 | Board Of Trustees Operating Michigan State University | Biocatalytic synthesis of quinic acid and conversion to hydroquinone |
| WO2000073484A1 (en) | 1999-05-28 | 2000-12-07 | University Of North Texas Health Science Center At Fort Worth | A global regulator used to enhance the synthesis of aromatic substances |
| US20050089974A1 (en) | 2000-10-27 | 2005-04-28 | Townsend Craig A. | Fermentative production of d-hydroxyphenylglycine and d-phenylglycine |
| RU2229514C2 (ru) | 2000-11-13 | 2004-05-27 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" | 3-дезокси-d-арабиногептулозонат-7-фосфатсинтаза и фрагмент днк, кодирующий 3-дезокси-d-арабиногептулозонат-7-фосфатсинтазу из methylophilus methylotrophus |
| KR101148255B1 (ko) | 2002-10-04 | 2012-08-08 | 다니스코 유에스 인크. | 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법 |
| US8206955B2 (en) | 2003-10-21 | 2012-06-26 | Cargill, Incorporated | Production of monatin and monatin precursors |
| CN1280417C (zh) * | 2004-11-01 | 2006-10-18 | 清华大学 | 一种人酪氨酸酶表达载体及其应用 |
| WO2007118682A1 (en) * | 2006-04-13 | 2007-10-25 | Dsm Ip Assets B.V. | Process for the preparation of enantiomeri cally enriched beta-amino alcohols starting from glycine and an aldehyde in the presence of a threonine aldolase and a decarboxylase |
| WO2007140339A2 (en) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Production of isoprenoids |
| US7700328B2 (en) | 2006-06-07 | 2010-04-20 | E.I. Du Pont De Nemours And Company | Method for producing an L-tyrosine over-producing bacterial strain |
| US8975063B2 (en) | 2006-10-19 | 2015-03-10 | California Institute Of Technology | Compositions and methods for producing benzylisoquinoline alkaloids |
| US20080102499A1 (en) | 2006-10-27 | 2008-05-01 | Lori Jean Templeton | Method of enhancing L-tyrosine production in recombinant bacteria |
| US20100047887A1 (en) | 2006-11-27 | 2010-02-25 | Jihane Achkar | Method for preparing hydroxytyrosol |
| WO2009046370A2 (en) | 2007-10-04 | 2009-04-09 | Bio Architecture Lab, Inc. | Biofuel production |
| CN105368764B (zh) | 2007-12-13 | 2019-03-12 | 丹尼斯科美国公司 | 用于生产异戊二烯的组合物和方法 |
| US20110045563A1 (en) | 2008-02-06 | 2011-02-24 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| WO2009132220A2 (en) | 2008-04-23 | 2009-10-29 | Danisco Us Inc. | Isoprene synthase variants for improved microbial production of isoprene |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| MX318543B (es) | 2008-07-02 | 2014-03-18 | Danisco Us Inc | Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros. |
| DE102008040352A1 (de) | 2008-07-11 | 2010-01-14 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Tryptophan unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| US20120252054A1 (en) | 2009-09-15 | 2012-10-04 | Sapphire Energy Inc | System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| KR20180014240A (ko) | 2009-10-23 | 2018-02-07 | 게노마티카 인코포레이티드 | 아닐린의 제조를 위한 미생물 |
| WO2012135389A2 (en) | 2011-03-28 | 2012-10-04 | The Regents Of The University Of California | Host cells and methods for oxidizing aromatic amino acids |
| ES2663445T3 (es) | 2012-01-30 | 2018-04-12 | Myriant Corporation | Producción de ácido mucónico a partir de microorganismos modificados genéticamente |
| US9234203B2 (en) | 2012-05-21 | 2016-01-12 | Cathay Industrial Biotech Ltd. | Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| MX374090B (es) | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| CN112410379B (zh) | 2013-11-04 | 2024-12-31 | 小利兰·斯坦福大学托管委员会 | 产生苄基异喹啉生物碱(bia)前体的微生物以及制备和使用所述前体的方法 |
| WO2015192233A1 (en) | 2014-06-19 | 2015-12-23 | Epimeron Inc. | Compositions and methods for making (s)-norcoclaurine and (s)-norlaudanosoline, and synthesis intermediates thereof |
| CN106795486B (zh) | 2014-08-21 | 2021-06-08 | 公益财团法人地球环境产业技术研究机构 | 棒状细菌转化体及使用该转化体的有机化合物的制造方法 |
| WO2016183023A1 (en) * | 2015-05-08 | 2016-11-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of producing epimerases and benzylisoquinoline alkaloids |
| WO2018203947A2 (en) | 2017-02-06 | 2018-11-08 | Zymergen Inc. | Engineered biosynthetic pathways for production of tyramine by fermentation |
-
2018
- 2018-02-06 WO PCT/US2018/017127 patent/WO2018203947A2/en not_active Ceased
- 2018-02-06 EP EP18758755.5A patent/EP3577220A2/en not_active Withdrawn
- 2018-02-06 KR KR1020197025667A patent/KR20190120238A/ko not_active Withdrawn
- 2018-02-06 JP JP2019542552A patent/JP2020513790A/ja active Pending
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2019
- 2019-06-26 US US16/453,648 patent/US11028416B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2850077C1 (ru) * | 2025-04-04 | 2025-11-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Горский государственный аграрный университет" | Штамм лактобактерий Enterococcus faecium 22-2024 ВКПМ В-14767 - продуцент молочной кислоты |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3577220A2 (en) | 2019-12-11 |
| JP2020513790A (ja) | 2020-05-21 |
| CN110494560A (zh) | 2019-11-22 |
| US20190390236A1 (en) | 2019-12-26 |
| WO2018203947A9 (en) | 2020-05-07 |
| KR20190120238A (ko) | 2019-10-23 |
| US11028416B2 (en) | 2021-06-08 |
| WO2018203947A2 (en) | 2018-11-08 |
| WO2018203947A3 (en) | 2019-01-17 |
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