CA3052357A1 - Procede de production d'anticorps multispecifiques - Google Patents

Procede de production d'anticorps multispecifiques Download PDF

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Publication number
CA3052357A1
CA3052357A1 CA3052357A CA3052357A CA3052357A1 CA 3052357 A1 CA3052357 A1 CA 3052357A1 CA 3052357 A CA3052357 A CA 3052357A CA 3052357 A CA3052357 A CA 3052357A CA 3052357 A1 CA3052357 A1 CA 3052357A1
Authority
CA
Canada
Prior art keywords
antibody
heavy chain
light chain
domain
antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3052357A
Other languages
English (en)
Inventor
Stefan Seeber
Ulrich Goepfert
Andrea Osterlehner
Hubert Kettenberger
Wolfgang Paul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA3052357A1 publication Critical patent/CA3052357A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2881—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD71
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency

Abstract

L'invention concerne un procédé de production d'un anticorps multispécifique comprenant les étapes consistant à fournir une cellule de mammifère exprimant l'anticorps, à transfecter ladite cellule de mammifère avec un vecteur d'expression comprenant une cassette d'expression codant pour un polypeptide de l'anticorps qui a un croisement de domaine, à cultiver la cellule transfectée et à récupérer l'anticorps à partir de la cellule ou du milieu de culture et à produire ainsi l'anticorps multispécifique.
CA3052357A 2017-03-10 2018-03-07 Procede de production d'anticorps multispecifiques Abandoned CA3052357A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17160415.0 2017-03-10
EP17160415 2017-03-10
PCT/EP2018/055532 WO2018162517A1 (fr) 2017-03-10 2018-03-07 Procédé de production d'anticorps multispécifiques

Publications (1)

Publication Number Publication Date
CA3052357A1 true CA3052357A1 (fr) 2018-09-13

Family

ID=58266981

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3052357A Abandoned CA3052357A1 (fr) 2017-03-10 2018-03-07 Procede de production d'anticorps multispecifiques

Country Status (18)

Country Link
US (2) US20200216553A1 (fr)
EP (1) EP3592767A1 (fr)
JP (2) JP7021245B2 (fr)
KR (2) KR20190124270A (fr)
CN (1) CN110402253B (fr)
AU (1) AU2018232698B2 (fr)
BR (1) BR112019016970A2 (fr)
CA (1) CA3052357A1 (fr)
CL (1) CL2019002521A1 (fr)
CR (1) CR20190397A (fr)
IL (1) IL268470A (fr)
MA (1) MA48723A (fr)
MX (1) MX2019010575A (fr)
NZ (1) NZ756132A (fr)
PE (1) PE20191360A1 (fr)
RU (1) RU2750721C2 (fr)
SG (1) SG11201908127WA (fr)
WO (1) WO2018162517A1 (fr)

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Publication number Priority date Publication date Assignee Title
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
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AU2020269597A1 (en) * 2019-05-07 2021-09-23 Amgen Inc. Vectors and expression systems for producing recombinant proteins
CA3140323A1 (fr) 2019-06-19 2020-12-24 Johannes Auer Procede de generation d'une cellule exprimant un anticorps multivalent, bispecifique par integration ciblee de multiples cassettes d'expression dans une organisation definie
KR20220024637A (ko) 2019-06-19 2022-03-03 에프. 호프만-라 로슈 아게 정의된 조직의 다수 발현 카세트들의 표적화 통합에 의한 3가 항체 발현 세포의 생성 방법
CA3140192A1 (fr) 2019-06-19 2020-12-24 Johannes Auer Procede de generation d'une cellule exprimant un anticorps multivalent, multispecifique par integration ciblee de multiples cassettes d'expression dans une organisation definie
EP3986926A1 (fr) 2019-06-19 2022-04-27 F. Hoffmann-La Roche AG Procédé de génération d'une cellule exprimant un anticorps bivalent bispécifique par intégration ciblée de multiples cassettes d'expression dans une organisation définie
WO2020254357A1 (fr) 2019-06-19 2020-12-24 F. Hoffmann-La Roche Ag Procédé de production d'une cellule exprimant une protéine par intégration ciblée à l'aide d'arnm de cre

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Also Published As

Publication number Publication date
CL2019002521A1 (es) 2020-01-31
JP2020509754A (ja) 2020-04-02
BR112019016970A2 (pt) 2020-04-07
JP7021245B2 (ja) 2022-02-16
MX2019010575A (es) 2019-10-15
JP2022001059A (ja) 2022-01-06
CR20190397A (es) 2019-09-27
AU2018232698A1 (en) 2019-08-15
RU2019131113A3 (fr) 2021-04-12
CN110402253B (zh) 2024-01-05
EP3592767A1 (fr) 2020-01-15
IL268470A (en) 2019-09-26
US20230129340A1 (en) 2023-04-27
CN110402253A (zh) 2019-11-01
PE20191360A1 (es) 2019-10-01
MA48723A (fr) 2020-04-08
US20200216553A1 (en) 2020-07-09
RU2750721C2 (ru) 2021-07-01
NZ756132A (en) 2022-02-25
AU2018232698B2 (en) 2020-06-25
KR20190124270A (ko) 2019-11-04
SG11201908127WA (en) 2019-10-30
RU2019131113A (ru) 2021-04-12
WO2018162517A1 (fr) 2018-09-13
KR20210132207A (ko) 2021-11-03

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