CA3044920C - In vitro glycoengineering of antibodies - Google Patents

In vitro glycoengineering of antibodies Download PDF

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Publication number
CA3044920C
CA3044920C CA3044920A CA3044920A CA3044920C CA 3044920 C CA3044920 C CA 3044920C CA 3044920 A CA3044920 A CA 3044920A CA 3044920 A CA3044920 A CA 3044920A CA 3044920 C CA3044920 C CA 3044920C
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CA
Canada
Prior art keywords
antibody
glycosylation
solution
ligand
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3044920A
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English (en)
French (fr)
Other versions
CA3044920A1 (en
Inventor
Roberto Falkenstein
Heiko Walch
Sebastian MALIK
Marco THOMANN
Matthias FREIHERR VON ROMAN
Ingrid Grunert
Roland DORN
Michael HINGAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=60997429&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA3044920(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA3044920A1 publication Critical patent/CA3044920A1/en
Application granted granted Critical
Publication of CA3044920C publication Critical patent/CA3044920C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA3044920A 2016-12-21 2017-12-19 In vitro glycoengineering of antibodies Active CA3044920C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP16205587 2016-12-21
EP16205587.5 2016-12-21
EP17157002 2017-02-20
EP17157002.1 2017-02-20
PCT/EP2017/083429 WO2018114877A1 (en) 2016-12-21 2017-12-19 In vitro glycoengineering of antibodies

Publications (2)

Publication Number Publication Date
CA3044920A1 CA3044920A1 (en) 2018-06-28
CA3044920C true CA3044920C (en) 2022-06-28

Family

ID=60997429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3044920A Active CA3044920C (en) 2016-12-21 2017-12-19 In vitro glycoengineering of antibodies

Country Status (11)

Country Link
US (2) US20200165320A1 (https=)
EP (1) EP3559248B2 (https=)
JP (1) JP6850351B2 (https=)
KR (1) KR102317884B1 (https=)
CN (2) CN119685431A (https=)
AU (1) AU2017384276B9 (https=)
CA (1) CA3044920C (https=)
IL (1) IL267351A (https=)
MX (1) MX2019006266A (https=)
TW (1) TWI780097B (https=)
WO (1) WO2018114877A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
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EP3559250A1 (en) 2016-12-21 2019-10-30 H. Hoffnabb-La Roche Ag Re-use of enzymes in in vitro glycoengineering of antibodies
WO2018114877A1 (en) 2016-12-21 2018-06-28 F. Hoffmann-La Roche Ag In vitro glycoengineering of antibodies
MX2019006123A (es) * 2016-12-21 2019-08-12 Hoffmann La Roche Metodo para glicomanipulacion in vitro de anticuerpos.
MA53122A (fr) 2018-07-13 2021-05-19 Genmab As Variants d'anticorps cd38 et leurs utilisations
JP7534281B2 (ja) 2018-07-13 2024-08-14 ジェンマブ エー／エス Ｃｄ３８抗体を使用したトロゴサイトーシスを介した治療
KR20220130724A (ko) 2020-01-16 2022-09-27 젠맵 에이/에스 Cd38 항체의 제제 및 그의 용도
KR20240051280A (ko) 2021-09-06 2024-04-19 젠맵 에이/에스 Cd27과 결합할 수 있는 항체, 그의 변이체 및 그의 용도
CN121285385A (zh) 2023-05-12 2026-01-06 金麦安博股份有限公司 能够与ox40结合的抗体、其变体及其用途
WO2025049881A1 (en) * 2023-09-01 2025-03-06 Catalent Pharma Solutions, Llc Dnase co-expression in host cells

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Also Published As

Publication number Publication date
BR112019010485A2 (pt) 2019-09-10
MX2019006266A (es) 2019-08-21
WO2018114877A1 (en) 2018-06-28
EP3559248B2 (en) 2024-11-20
US20200165320A1 (en) 2020-05-28
CN119685431A (zh) 2025-03-25
AU2017384276A1 (en) 2019-05-30
EP3559248B1 (en) 2021-11-17
AU2017384276B9 (en) 2020-11-26
KR20190082942A (ko) 2019-07-10
TWI780097B (zh) 2022-10-11
EP3559248A1 (en) 2019-10-30
JP6850351B2 (ja) 2021-03-31
IL267351A (en) 2019-08-29
KR102317884B1 (ko) 2021-10-26
CA3044920A1 (en) 2018-06-28
TW201835333A (zh) 2018-10-01
AU2017384276B2 (en) 2020-07-02
CN110088291A (zh) 2019-08-02
US20240101643A1 (en) 2024-03-28
JP2020501577A (ja) 2020-01-23

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Effective date: 20190524