CA3038122A1 - Methods and systems for determining adamts13 enzyme activity - Google Patents

Methods and systems for determining adamts13 enzyme activity Download PDF

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Publication number
CA3038122A1
CA3038122A1 CA3038122A CA3038122A CA3038122A1 CA 3038122 A1 CA3038122 A1 CA 3038122A1 CA 3038122 A CA3038122 A CA 3038122A CA 3038122 A CA3038122 A CA 3038122A CA 3038122 A1 CA3038122 A1 CA 3038122A1
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CA
Canada
Prior art keywords
enzymatic cleavage
sample
cleavage product
adamts13
amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3038122A
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English (en)
French (fr)
Inventor
Russell Philip Grant
Christopher Michael SHUFORD
Megan Norris BRADLEY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Laboratory Corp of America Holdings
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Laboratory Corp of America Holdings
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Filing date
Publication date
Application filed by Laboratory Corp of America Holdings filed Critical Laboratory Corp of America Holdings
Publication of CA3038122A1 publication Critical patent/CA3038122A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/22—Haematology
    • G01N2800/226—Thrombotic disorders, i.e. thrombo-embolism irrespective of location/organ involved, e.g. renal vein thrombosis, venous thrombosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Optics & Photonics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3038122A 2016-10-11 2017-10-11 Methods and systems for determining adamts13 enzyme activity Pending CA3038122A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662406693P 2016-10-11 2016-10-11
US62/406,693 2016-10-11
PCT/US2017/056080 WO2018071502A1 (en) 2016-10-11 2017-10-11 Methods and systems for determining adamts13 enzyme activity

Publications (1)

Publication Number Publication Date
CA3038122A1 true CA3038122A1 (en) 2018-04-19

Family

ID=60186390

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3038122A Pending CA3038122A1 (en) 2016-10-11 2017-10-11 Methods and systems for determining adamts13 enzyme activity

Country Status (8)

Country Link
US (2) US10858689B2 (ko)
EP (1) EP3526339A1 (ko)
JP (2) JP2019532649A (ko)
KR (1) KR102386584B1 (ko)
CN (1) CN110139935A (ko)
AU (1) AU2017343623A1 (ko)
CA (1) CA3038122A1 (ko)
WO (1) WO2018071502A1 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10858689B2 (en) 2016-10-11 2020-12-08 Laboratory Corporation Of America Holdings Methods and systems for determining ADAMTS13 enzyme activity
CN110760563A (zh) * 2019-10-10 2020-02-07 北京东西分析仪器有限公司 测定酶活性的方法
CN110627913A (zh) * 2019-11-06 2019-12-31 浙江壹晨生物科技有限公司 一种带赖氨酸标签的adamts13底物及其制备方法和应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003284570A (ja) * 2001-04-25 2003-10-07 Chemo Sero Therapeut Res Inst フォンビルブラント因子（ｖＷＦ）切断酵素
JP3944586B2 (ja) 2002-10-18 2007-07-11 国立循環器病センター総長 フォンビルブランド因子切断酵素の特異的基質および活性測定法
DE602004027314D1 (de) 2004-07-19 2010-07-01 American Diagnostica Inc Verfahren zur messung von adamts13-aktivität auf der blutplättchenoberfläche.
US7833726B2 (en) 2005-02-14 2010-11-16 Alfresa Pharma Corporation Antibody for assaying ADAMTS13 activity and method for assaying the activity
US20090004673A1 (en) 2006-01-31 2009-01-01 Mitsubishi Kagaku Iatron, Inc. Method for Determining Condition of Disseminated Intravascular Coagulation
JP5221333B2 (ja) 2006-02-16 2013-06-26 三菱化学メディエンス株式会社 意識障害患者における病態の検出方法及び検出用キット
US7893616B2 (en) 2006-02-27 2011-02-22 Ohio State University Process to determine enzyme activity
JP4820192B2 (ja) 2006-03-17 2011-11-24 一般財団法人化学及血清療法研究所 急性冠症候群でのａｄａｍｔｓ１３の測定と利用
DE102007031708A1 (de) 2007-07-06 2009-01-08 Dade Behring Marburg Gmbh Bestimmung der von Willebrand Faktor-Aktivität in Abwesenheit von Ristocetin
KR101670103B1 (ko) 2008-12-05 2016-10-27 박스알타 인코퍼레이티드 폰빌레브란트 인자의 ａｄａｍｔｓ１３－매개 생체내 절단을 측정하는 방법 및 그의 사용
CA2757585C (en) * 2010-11-17 2022-07-19 Puget Sound Blood Center Enhanced cleavage of von willebrand factor by adamts13
JP6419576B2 (ja) 2011-11-11 2018-11-07 イムコア ジーティーアイ ダイアグノスティクス インコーポレイテッド プロテアーゼａｄａｍｔｓ１３を切断するフォン・ヴィレブランド因子の検出用ポリペプチド基体
CN102533937A (zh) * 2012-02-03 2012-07-04 苏州大学附属第一医院 一种检测adamts13酶活性的荧光底物及检测方法
EP3401685B1 (en) 2016-01-08 2021-12-01 Kyoto University Diagnostic method and medicine comprising adamts13 as main ingredient
US10858689B2 (en) 2016-10-11 2020-12-08 Laboratory Corporation Of America Holdings Methods and systems for determining ADAMTS13 enzyme activity
CN106771230A (zh) 2016-11-11 2017-05-31 厦门文武天成生物科技有限公司 利用seldi‑tof‑ms检测血清中adamts13酶活性的方法
US20190369095A1 (en) 2016-11-30 2019-12-05 Helga Vetr Novel antibody for determination of adamts-13 activity
GB201707139D0 (en) 2017-05-04 2017-06-21 Imp Innovations Ltd Polypeptides

Also Published As

Publication number Publication date
US11959124B2 (en) 2024-04-16
US20180163249A1 (en) 2018-06-14
EP3526339A1 (en) 2019-08-21
US20210062242A1 (en) 2021-03-04
WO2018071502A1 (en) 2018-04-19
US10858689B2 (en) 2020-12-08
AU2017343623A1 (en) 2019-04-18
CN110139935A (zh) 2019-08-16
AU2017343623A8 (en) 2023-03-30
KR20190067844A (ko) 2019-06-17
JP2022123057A (ja) 2022-08-23
JP2019532649A (ja) 2019-11-14
KR102386584B1 (ko) 2022-04-14

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