CA2973361A1 - Microscopie sted a balayage en ligne multicanal, methode et appareil - Google Patents

Microscopie sted a balayage en ligne multicanal, methode et appareil Download PDF

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Publication number
CA2973361A1
CA2973361A1 CA2973361A CA2973361A CA2973361A1 CA 2973361 A1 CA2973361 A1 CA 2973361A1 CA 2973361 A CA2973361 A CA 2973361A CA 2973361 A CA2973361 A CA 2973361A CA 2973361 A1 CA2973361 A1 CA 2973361A1
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Canada
Prior art keywords
light
sted
sample
imaging
scanning
Prior art date
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Abandoned
Application number
CA2973361A
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English (en)
Inventor
Peter A. Vokhmin
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Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA2973361A priority Critical patent/CA2973361A1/fr
Publication of CA2973361A1 publication Critical patent/CA2973361A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2973361A 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil Abandoned CA2973361A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2973361A CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2973361A CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Publications (1)

Publication Number Publication Date
CA2973361A1 true CA2973361A1 (fr) 2019-01-14

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ID=65019155

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2973361A Abandoned CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Country Status (1)

Country Link
CA (1) CA2973361A1 (fr)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110068554A (zh) * 2019-04-24 2019-07-30 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110187487A (zh) * 2019-06-13 2019-08-30 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN111182238A (zh) * 2019-11-15 2020-05-19 北京超放信息技术有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111521608A (zh) * 2020-04-27 2020-08-11 中国科学院广州生物医药与健康研究院 一种超分辨率显微成像方法及显微镜
CN111879737A (zh) * 2019-09-10 2020-11-03 之江实验室 一种产生高通量超衍射极限焦斑的装置和方法
CN111912796A (zh) * 2019-12-12 2020-11-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN113504634A (zh) * 2021-06-30 2021-10-15 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN113532271A (zh) * 2021-05-31 2021-10-22 浙江大学 一种无标记三维超分辨显微方法与装置
WO2022005286A1 (fr) * 2020-07-03 2022-01-06 Confocal.Nl B.V. Systèmes et procédés optiques de rébalayage
CN114019764A (zh) * 2021-10-25 2022-02-08 之江实验室 一种超分辨激光直写与成像方法及装置
CN114216887A (zh) * 2021-12-02 2022-03-22 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法
WO2022106468A1 (fr) * 2020-11-18 2022-05-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé et microscope à balayage laser pour l'imagerie d'une structure d'un échantillon marqué par différents marqueurs fluorescents

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110068554A (zh) * 2019-04-24 2019-07-30 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110068554B (zh) * 2019-04-24 2021-12-17 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110187487A (zh) * 2019-06-13 2019-08-30 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN110187487B (zh) * 2019-06-13 2021-07-27 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN111879737A (zh) * 2019-09-10 2020-11-03 之江实验室 一种产生高通量超衍射极限焦斑的装置和方法
CN111182238A (zh) * 2019-11-15 2020-05-19 北京超放信息技术有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111182238B (zh) * 2019-11-15 2023-04-18 浙江荷湖科技有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111912796A (zh) * 2019-12-12 2020-11-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN111912796B (zh) * 2019-12-12 2023-03-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN111521608A (zh) * 2020-04-27 2020-08-11 中国科学院广州生物医药与健康研究院 一种超分辨率显微成像方法及显微镜
WO2022005286A1 (fr) * 2020-07-03 2022-01-06 Confocal.Nl B.V. Systèmes et procédés optiques de rébalayage
NL2026002B1 (en) * 2020-07-03 2022-03-08 Confocal Nl B V Re-scan optical systems and methods
WO2022106468A1 (fr) * 2020-11-18 2022-05-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé et microscope à balayage laser pour l'imagerie d'une structure d'un échantillon marqué par différents marqueurs fluorescents
CN113532271A (zh) * 2021-05-31 2021-10-22 浙江大学 一种无标记三维超分辨显微方法与装置
CN113504634B (zh) * 2021-06-30 2023-01-03 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN113504634A (zh) * 2021-06-30 2021-10-15 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN114019764A (zh) * 2021-10-25 2022-02-08 之江实验室 一种超分辨激光直写与成像方法及装置
CN114019764B (zh) * 2021-10-25 2024-01-09 之江实验室 一种超分辨激光直写与成像方法及装置
CN114216887A (zh) * 2021-12-02 2022-03-22 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法
CN114216887B (zh) * 2021-12-02 2023-11-28 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法

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Effective date: 20200831