CA2973361A1 - Microscopie sted a balayage en ligne multicanal, methode et appareil - Google Patents

Microscopie sted a balayage en ligne multicanal, methode et appareil Download PDF

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Publication number
CA2973361A1
CA2973361A1 CA2973361A CA2973361A CA2973361A1 CA 2973361 A1 CA2973361 A1 CA 2973361A1 CA 2973361 A CA2973361 A CA 2973361A CA 2973361 A CA2973361 A CA 2973361A CA 2973361 A1 CA2973361 A1 CA 2973361A1
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CA
Canada
Prior art keywords
light
sted
sample
imaging
scanning
Prior art date
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Abandoned
Application number
CA2973361A
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English (en)
Inventor
Peter A. Vokhmin
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA2973361A priority Critical patent/CA2973361A1/fr
Publication of CA2973361A1 publication Critical patent/CA2973361A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2973361A 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil Abandoned CA2973361A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2973361A CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2973361A CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Publications (1)

Publication Number Publication Date
CA2973361A1 true CA2973361A1 (fr) 2019-01-14

Family

ID=65019155

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2973361A Abandoned CA2973361A1 (fr) 2017-07-14 2017-07-14 Microscopie sted a balayage en ligne multicanal, methode et appareil

Country Status (1)

Country Link
CA (1) CA2973361A1 (fr)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110068554A (zh) * 2019-04-24 2019-07-30 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110187487A (zh) * 2019-06-13 2019-08-30 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN111182238A (zh) * 2019-11-15 2020-05-19 北京超放信息技术有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111521608A (zh) * 2020-04-27 2020-08-11 中国科学院广州生物医药与健康研究院 一种超分辨率显微成像方法及显微镜
CN111879737A (zh) * 2019-09-10 2020-11-03 之江实验室 一种产生高通量超衍射极限焦斑的装置和方法
CN111912796A (zh) * 2019-12-12 2020-11-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN113504634A (zh) * 2021-06-30 2021-10-15 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN113532271A (zh) * 2021-05-31 2021-10-22 浙江大学 一种无标记三维超分辨显微方法与装置
WO2022005286A1 (fr) * 2020-07-03 2022-01-06 Confocal.Nl B.V. Systèmes et procédés optiques de rébalayage
CN114019764A (zh) * 2021-10-25 2022-02-08 之江实验室 一种超分辨激光直写与成像方法及装置
CN114216887A (zh) * 2021-12-02 2022-03-22 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法
WO2022106468A1 (fr) * 2020-11-18 2022-05-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé et microscope à balayage laser pour l'imagerie d'une structure d'un échantillon marqué par différents marqueurs fluorescents

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110068554A (zh) * 2019-04-24 2019-07-30 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110068554B (zh) * 2019-04-24 2021-12-17 暨南大学 一种小尺寸等离子纳米颗粒探测的超分辨显微系统
CN110187487A (zh) * 2019-06-13 2019-08-30 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN110187487B (zh) * 2019-06-13 2021-07-27 福建师范大学 单波长双光子sted与双波长单光子sted耦合成像装置及方法
CN111879737A (zh) * 2019-09-10 2020-11-03 之江实验室 一种产生高通量超衍射极限焦斑的装置和方法
CN111182238A (zh) * 2019-11-15 2020-05-19 北京超放信息技术有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111182238B (zh) * 2019-11-15 2023-04-18 浙江荷湖科技有限公司 基于扫描光场的高分辨率移动电子设备的成像装置及方法
CN111912796A (zh) * 2019-12-12 2020-11-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN111912796B (zh) * 2019-12-12 2023-03-10 南开大学 一种基于可见吸收光谱的超分辨成像系统和方法
CN111521608A (zh) * 2020-04-27 2020-08-11 中国科学院广州生物医药与健康研究院 一种超分辨率显微成像方法及显微镜
WO2022005286A1 (fr) * 2020-07-03 2022-01-06 Confocal.Nl B.V. Systèmes et procédés optiques de rébalayage
NL2026002B1 (en) * 2020-07-03 2022-03-08 Confocal Nl B V Re-scan optical systems and methods
WO2022106468A1 (fr) * 2020-11-18 2022-05-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé et microscope à balayage laser pour l'imagerie d'une structure d'un échantillon marqué par différents marqueurs fluorescents
CN113532271A (zh) * 2021-05-31 2021-10-22 浙江大学 一种无标记三维超分辨显微方法与装置
CN113504634B (zh) * 2021-06-30 2023-01-03 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN113504634A (zh) * 2021-06-30 2021-10-15 华南师范大学 点扫描长寿命荧光显微成像的智能高速扫描方法及装置
CN114019764A (zh) * 2021-10-25 2022-02-08 之江实验室 一种超分辨激光直写与成像方法及装置
CN114019764B (zh) * 2021-10-25 2024-01-09 之江实验室 一种超分辨激光直写与成像方法及装置
CN114216887A (zh) * 2021-12-02 2022-03-22 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法
CN114216887B (zh) * 2021-12-02 2023-11-28 南昌大学 偏振调制提高受激发射损耗显微系统分辨率的方法

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Effective date: 20200831