CA3035876A1 - Systemes de reassignation microscopiques a balayage de ligne photonique - Google Patents
Systemes de reassignation microscopiques a balayage de ligne photonique Download PDFInfo
- Publication number
- CA3035876A1 CA3035876A1 CA3035876A CA3035876A CA3035876A1 CA 3035876 A1 CA3035876 A1 CA 3035876A1 CA 3035876 A CA3035876 A CA 3035876A CA 3035876 A CA3035876 A CA 3035876A CA 3035876 A1 CA3035876 A1 CA 3035876A1
- Authority
- CA
- Canada
- Prior art keywords
- scan
- sample
- plane
- microscopy
- imaging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000386 microscopy Methods 0.000 title claims abstract description 80
- 230000005284 excitation Effects 0.000 claims abstract description 98
- 238000003384 imaging method Methods 0.000 claims abstract description 85
- 230000003287 optical effect Effects 0.000 claims abstract description 77
- 238000001514 detection method Methods 0.000 claims abstract description 39
- 230000005855 radiation Effects 0.000 claims abstract description 21
- 239000000523 sample Substances 0.000 claims description 84
- 238000005286 illumination Methods 0.000 claims description 24
- 238000000799 fluorescence microscopy Methods 0.000 claims description 13
- 230000000903 blocking effect Effects 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 230000001360 synchronised effect Effects 0.000 claims description 8
- 238000012805 post-processing Methods 0.000 claims description 7
- 230000005684 electric field Effects 0.000 claims description 6
- 230000010287 polarization Effects 0.000 claims description 6
- 238000011144 upstream manufacturing Methods 0.000 claims description 4
- 230000002146 bilateral effect Effects 0.000 claims description 3
- 239000007850 fluorescent dye Substances 0.000 claims description 3
- 238000001454 recorded image Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 38
- 238000003491 array Methods 0.000 abstract description 5
- 230000006870 function Effects 0.000 description 19
- 238000004624 confocal microscopy Methods 0.000 description 16
- 102100027780 Splicing factor, proline- and glutamine-rich Human genes 0.000 description 10
- 229920013655 poly(bisphenol-A sulfone) Polymers 0.000 description 10
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 7
- 238000004621 scanning probe microscopy Methods 0.000 description 5
- 238000013459 approach Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 238000002073 fluorescence micrograph Methods 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
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- 238000001000 micrograph Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000021251 pulses Nutrition 0.000 description 2
- 210000001747 pupil Anatomy 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000007493 shaping process Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 208000037063 Thinness Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 201000009310 astigmatism Diseases 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010226 confocal imaging Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000010869 super-resolution microscopy Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000000482 two photon fluorescence microscopy Methods 0.000 description 1
- 206010048828 underweight Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B3/00—Simple or compound lenses
- G02B3/0006—Arrays
- G02B3/0037—Arrays characterized by the distribution or form of lenses
- G02B3/005—Arrays characterized by the distribution or form of lenses arranged along a single direction only, e.g. lenticular sheets
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Analytical Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Multimedia (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3035876A CA3035876A1 (fr) | 2019-03-06 | 2019-03-06 | Systemes de reassignation microscopiques a balayage de ligne photonique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3035876A CA3035876A1 (fr) | 2019-03-06 | 2019-03-06 | Systemes de reassignation microscopiques a balayage de ligne photonique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3035876A1 true CA3035876A1 (fr) | 2020-09-06 |
Family
ID=72424891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3035876A Abandoned CA3035876A1 (fr) | 2019-03-06 | 2019-03-06 | Systemes de reassignation microscopiques a balayage de ligne photonique |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA3035876A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114296228A (zh) * | 2021-12-31 | 2022-04-08 | 南京理工大学智能计算成像研究院有限公司 | 一种基于混合照明模式的高分辨率显微成像方法 |
| WO2023287978A1 (fr) * | 2021-07-15 | 2023-01-19 | Trustees Of Boston University | Multiplicateur de balayage optique et ses utilisations |
-
2019
- 2019-03-06 CA CA3035876A patent/CA3035876A1/fr not_active Abandoned
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023287978A1 (fr) * | 2021-07-15 | 2023-01-19 | Trustees Of Boston University | Multiplicateur de balayage optique et ses utilisations |
| CN114296228A (zh) * | 2021-12-31 | 2022-04-08 | 南京理工大学智能计算成像研究院有限公司 | 一种基于混合照明模式的高分辨率显微成像方法 |
| CN114296228B (zh) * | 2021-12-31 | 2024-01-23 | 南京理工大学智能计算成像研究院有限公司 | 一种基于混合照明模式的高分辨率显微成像方法 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20220908 |
|
| FZDE | Discontinued |
Effective date: 20220908 |