CA2978123A1 - Systeme de microscopie sted multicanal en temps reel - Google Patents

Systeme de microscopie sted multicanal en temps reel Download PDF

Info

Publication number
CA2978123A1
CA2978123A1 CA2978123A CA2978123A CA2978123A1 CA 2978123 A1 CA2978123 A1 CA 2978123A1 CA 2978123 A CA2978123 A CA 2978123A CA 2978123 A CA2978123 A CA 2978123A CA 2978123 A1 CA2978123 A1 CA 2978123A1
Authority
CA
Canada
Prior art keywords
imaging
light
sted
radiation
beamlets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2978123A
Other languages
English (en)
Inventor
Peter Vokhmin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA2978123A priority Critical patent/CA2978123A1/fr
Publication of CA2978123A1 publication Critical patent/CA2978123A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0044—Scanning details, e.g. scanning stages moving apertures, e.g. Nipkow disks, rotating lens arrays
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
CA2978123A 2017-09-05 2017-09-05 Systeme de microscopie sted multicanal en temps reel Abandoned CA2978123A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2978123A CA2978123A1 (fr) 2017-09-05 2017-09-05 Systeme de microscopie sted multicanal en temps reel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2978123A CA2978123A1 (fr) 2017-09-05 2017-09-05 Systeme de microscopie sted multicanal en temps reel

Publications (1)

Publication Number Publication Date
CA2978123A1 true CA2978123A1 (fr) 2019-03-05

Family

ID=65632965

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2978123A Abandoned CA2978123A1 (fr) 2017-09-05 2017-09-05 Systeme de microscopie sted multicanal en temps reel

Country Status (1)

Country Link
CA (1) CA2978123A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111122526A (zh) * 2019-12-18 2020-05-08 中国科学院南海海洋研究所 一种基于激光诱导荧光及流体动态显微摄影的水体藻类检测方法
CN111474150A (zh) * 2020-04-08 2020-07-31 华南师范大学 一种sted超分辨图像背景噪声差分抑制方法
CN111653378A (zh) * 2020-06-05 2020-09-11 桂林电子科技大学 基于多光纤光镊的sted超分辨显微成像装置
EP3888838A1 (fr) * 2020-03-12 2021-10-06 Rohr, Inc. Système et procédé de perforation de substrat utilisant des mini-faisceaux et un scanner
CN114280766A (zh) * 2021-12-24 2022-04-05 哈尔滨工业大学 基于梯度折射率透镜的七目微型三维显微成像装置和方法
CN116068745A (zh) * 2023-02-24 2023-05-05 广东粤港澳大湾区协同创新研究院 一种角度连续可调超分辨显微镜照明装置及相应成像方法
EP4258036A1 (fr) * 2022-04-07 2023-10-11 Till I.D. GmbH Dispositif de microscope à disque tournant avec une résolution d'image potentiellement améliorée

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111122526A (zh) * 2019-12-18 2020-05-08 中国科学院南海海洋研究所 一种基于激光诱导荧光及流体动态显微摄影的水体藻类检测方法
EP3888838A1 (fr) * 2020-03-12 2021-10-06 Rohr, Inc. Système et procédé de perforation de substrat utilisant des mini-faisceaux et un scanner
CN111474150A (zh) * 2020-04-08 2020-07-31 华南师范大学 一种sted超分辨图像背景噪声差分抑制方法
CN111474150B (zh) * 2020-04-08 2022-03-22 华南师范大学 一种sted超分辨图像背景噪声差分抑制方法
CN111653378A (zh) * 2020-06-05 2020-09-11 桂林电子科技大学 基于多光纤光镊的sted超分辨显微成像装置
CN114280766A (zh) * 2021-12-24 2022-04-05 哈尔滨工业大学 基于梯度折射率透镜的七目微型三维显微成像装置和方法
EP4258036A1 (fr) * 2022-04-07 2023-10-11 Till I.D. GmbH Dispositif de microscope à disque tournant avec une résolution d'image potentiellement améliorée
CN116068745A (zh) * 2023-02-24 2023-05-05 广东粤港澳大湾区协同创新研究院 一种角度连续可调超分辨显微镜照明装置及相应成像方法

Similar Documents

Publication Publication Date Title
CA2978123A1 (fr) Systeme de microscopie sted multicanal en temps reel
Vangindertael et al. An introduction to optical super-resolution microscopy for the adventurous biologist
US8705172B2 (en) Microscopy method and microscope with enhanced resolution
CN107941763B (zh) 一种共轴三维受激辐射损耗超分辨显微成像方法和装置
US7170675B2 (en) Method and system for wide-field multi-photon microscopy having a confocal excitation plane
US6028306A (en) Scanning microscope
Inoué Foundations of confocal scanned imaging in light microscopy
US20200150446A1 (en) Method and System for Improving Lateral Resolution in Optical Scanning Microscopy
US9201008B2 (en) Method and system for obtaining an extended-depth-of-field volumetric image using laser scanning imaging
US8362448B2 (en) Apparatus and method for high spatial resolution imaging of a structure of a sample
JP5485352B2 (ja) 解像度を高めたルミネセンス顕微鏡検査
CA2973361A1 (fr) Microscopie sted a balayage en ligne multicanal, methode et appareil
JP6996048B2 (ja) 広視野高分解能顕微鏡
WO2016054118A1 (fr) Microscopie à éclairage structuré non linéaire
DE102009043744A1 (de) Verfahren und Mikroskop zur dreidimensional auflösungsgesteigerten Mikroskopie
Qu et al. Recent progress in multifocal multiphoton microscopy
JP7090930B2 (ja) 超解像光学顕微イメージングシステム
Jonkman et al. [18] Resolution in optical microscopy
Chitnis et al. Time-lapse imaging beyond the diffraction limit
US20180196245A1 (en) High-resolution spectrally selective scanning microscopy of a sample
Fuseler et al. Types of confocal instruments: basic principles and advantages and disadvantages
CA3035876A1 (fr) Systemes de reassignation microscopiques a balayage de ligne photonique
US11073477B2 (en) Epi-cone shell light-sheet super-resolution system and microscope
Martini et al. Scattering suppression and confocal detection in multifocal multiphoton microscopy
Botcherby et al. Arbitrary-scan imaging for two-photon microscopy

Legal Events

Date Code Title Description
FZDE Dead

Effective date: 20200908