CA2972575A1 - Rpe cell populations and methods of generating same - Google Patents

Rpe cell populations and methods of generating same Download PDF

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Publication number
CA2972575A1
CA2972575A1 CA2972575A CA2972575A CA2972575A1 CA 2972575 A1 CA2972575 A1 CA 2972575A1 CA 2972575 A CA2972575 A CA 2972575A CA 2972575 A CA2972575 A CA 2972575A CA 2972575 A1 CA2972575 A1 CA 2972575A1
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Prior art keywords
cells
rpe
cell population
cell
medium
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Pending
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CA2972575A
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English (en)
French (fr)
Inventor
Osnat BOHANA-KASHTAN
Lior Ann ROSENBERG BELMAKER
Ofer WISER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Cure Neurosciences Ltd
Original Assignee
Bohana-Kashtan Osnat
Rosenberg Belmaker Lior Ann
Cell Cure Neurosciences Ltd
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Application filed by Bohana-Kashtan Osnat, Rosenberg Belmaker Lior Ann, Cell Cure Neurosciences Ltd filed Critical Bohana-Kashtan Osnat
Publication of CA2972575A1 publication Critical patent/CA2972575A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0621Eye cells, e.g. cornea, iris pigmented cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00Drugs for disorders of the senses
    • A61P27/02Ophthalmic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Ophthalmology & Optometry (AREA)
  • Wood Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2972575A 2014-12-30 2015-12-30 Rpe cell populations and methods of generating same Pending CA2972575A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462097753P 2014-12-30 2014-12-30
US62/097,753 2014-12-30
US201562116972P 2015-02-17 2015-02-17
US62/116,972 2015-02-17
US201562195309P 2015-07-22 2015-07-22
US62/195,309 2015-07-22
PCT/IL2015/051269 WO2016108239A1 (en) 2014-12-30 2015-12-30 Rpe cell populations and methods of generating same

Publications (1)

Publication Number Publication Date
CA2972575A1 true CA2972575A1 (en) 2016-07-07

Family

ID=55315463

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2972575A Pending CA2972575A1 (en) 2014-12-30 2015-12-30 Rpe cell populations and methods of generating same

Country Status (17)

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US (6) US11891622B2 (cg-RX-API-DMAC7.html)
EP (2) EP3240892B9 (cg-RX-API-DMAC7.html)
JP (4) JP6882981B2 (cg-RX-API-DMAC7.html)
KR (1) KR20170115514A (cg-RX-API-DMAC7.html)
CN (1) CN107406830A (cg-RX-API-DMAC7.html)
AU (3) AU2015373050B2 (cg-RX-API-DMAC7.html)
BR (1) BR112017014345A2 (cg-RX-API-DMAC7.html)
CA (1) CA2972575A1 (cg-RX-API-DMAC7.html)
DK (1) DK3240892T5 (cg-RX-API-DMAC7.html)
EA (1) EA201791416A1 (cg-RX-API-DMAC7.html)
ES (1) ES2774456T3 (cg-RX-API-DMAC7.html)
HK (1) HK1246348A1 (cg-RX-API-DMAC7.html)
IL (4) IL273405B2 (cg-RX-API-DMAC7.html)
MX (1) MX2017008737A (cg-RX-API-DMAC7.html)
PH (1) PH12017501218A1 (cg-RX-API-DMAC7.html)
SG (1) SG11201705379VA (cg-RX-API-DMAC7.html)
WO (1) WO2016108239A1 (cg-RX-API-DMAC7.html)

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US10485829B2 (en) 2009-11-17 2019-11-26 Astellas Institute For Regenerative Medicine Methods of producing human RPE cells and pharmaceutical preparations of human RPE cells
IL273405B2 (en) 2014-12-30 2024-01-01 Cell Cure Neurosciences Ltd Rpe cell populations and methods of generating same
IL253244B2 (en) 2014-12-30 2023-09-01 Cell Cure Neurosciences Ltd Methods for treating retinal diseases
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BR112020013238A2 (pt) * 2017-12-29 2020-12-01 Cell Cure Neurosciences Ltd. composição e método para formular células do epitélio pigmentar da retina bem como uso da dita composição para tratar uma condição retiniana
CN113811605B (zh) * 2019-05-15 2024-09-27 诺和诺德股份有限公司 从人多能干细胞获得眼野祖细胞的方法
CN111972399B (zh) * 2020-08-06 2021-11-30 温州医科大学 维持肝细胞活性的保存液
WO2023009676A1 (en) 2021-07-28 2023-02-02 Lineage Cell Therapeutics, Inc. Expansion of retinal pigment epithelium cells
CN115840045B (zh) * 2023-02-23 2023-04-28 中国农业大学 一种猪胚胎期毛囊基板前体细胞的鉴定方法及其应用

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