CA2967811A1 - Adapter for a cell holder of a spectrofluorometer - Google Patents
Adapter for a cell holder of a spectrofluorometer Download PDFInfo
- Publication number
- CA2967811A1 CA2967811A1 CA2967811A CA2967811A CA2967811A1 CA 2967811 A1 CA2967811 A1 CA 2967811A1 CA 2967811 A CA2967811 A CA 2967811A CA 2967811 A CA2967811 A CA 2967811A CA 2967811 A1 CA2967811 A1 CA 2967811A1
- Authority
- CA
- Canada
- Prior art keywords
- microcell
- adapter
- cell
- excitation
- emission
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000005284 excitation Effects 0.000 claims abstract description 137
- 238000000034 method Methods 0.000 claims abstract description 38
- 239000000232 Lipid Bilayer Substances 0.000 claims description 56
- 238000004458 analytical method Methods 0.000 claims description 24
- 238000011010 flushing procedure Methods 0.000 claims description 15
- 239000000725 suspension Substances 0.000 claims description 5
- 238000001917 fluorescence detection Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 182
- 239000000243 solution Substances 0.000 description 37
- 239000000523 sample Substances 0.000 description 29
- 210000002421 cell wall Anatomy 0.000 description 27
- 230000003287 optical effect Effects 0.000 description 18
- 150000002632 lipids Chemical class 0.000 description 17
- 238000005259 measurement Methods 0.000 description 17
- 238000005286 illumination Methods 0.000 description 14
- 239000011521 glass Substances 0.000 description 13
- 150000003904 phospholipids Chemical class 0.000 description 10
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 10
- 239000012528 membrane Substances 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 239000003446 ligand Substances 0.000 description 7
- 108010052285 Membrane Proteins Proteins 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 239000008364 bulk solution Substances 0.000 description 5
- 239000002502 liposome Substances 0.000 description 5
- 239000010453 quartz Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000000799 fluorescence microscopy Methods 0.000 description 4
- 238000001506 fluorescence spectroscopy Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 238000000386 microscopy Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000003595 spectral effect Effects 0.000 description 4
- 238000000295 emission spectrum Methods 0.000 description 3
- 210000002287 horizontal cell Anatomy 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 102000018697 Membrane Proteins Human genes 0.000 description 2
- LOUPRKONTZGTKE-WZBLMQSHSA-N Quinine Chemical compound C([C@H]([C@H](C1)C=C)C2)C[N@@]1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-WZBLMQSHSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000004624 confocal microscopy Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 239000002691 unilamellar liposome Substances 0.000 description 2
- DSNRWDQKZIEDDB-SQYFZQSCSA-N 1,2-dioleoyl-sn-glycero-3-phospho-(1'-sn-glycerol) Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@@H](O)CO)OC(=O)CCCCCCC\C=C/CCCCCCCC DSNRWDQKZIEDDB-SQYFZQSCSA-N 0.000 description 1
- SNKAWJBJQDLSFF-NVKMUCNASA-N 1,2-dioleoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC SNKAWJBJQDLSFF-NVKMUCNASA-N 0.000 description 1
- 235000001258 Cinchona calisaya Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- LOUPRKONTZGTKE-UHFFFAOYSA-N cinchonine Natural products C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108010030416 proteoliposomes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229960000948 quinine Drugs 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/064—Stray light conditioning
- G01N2201/0648—Shutters
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462079273P | 2014-11-13 | 2014-11-13 | |
| US62/079,273 | 2014-11-13 | ||
| US201562186449P | 2015-06-30 | 2015-06-30 | |
| US62/186,449 | 2015-06-30 | ||
| PCT/US2015/060658 WO2016077741A1 (en) | 2014-11-13 | 2015-11-13 | Adapter for a cell holder of a spectrofluorometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2967811A1 true CA2967811A1 (en) | 2016-05-19 |
Family
ID=55955136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2967811A Abandoned CA2967811A1 (en) | 2014-11-13 | 2015-11-13 | Adapter for a cell holder of a spectrofluorometer |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US10139344B2 (enExample) |
| EP (1) | EP3218680A4 (enExample) |
| JP (1) | JP6659713B2 (enExample) |
| CA (1) | CA2967811A1 (enExample) |
| WO (1) | WO2016077741A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6829466B2 (ja) * | 2017-03-24 | 2021-02-10 | 株式会社日立ハイテクサイエンス | 分光蛍光光度計 |
| US10371633B2 (en) * | 2017-10-30 | 2019-08-06 | Saudi Arabian Oil Company | Determining a specific gravity of a sample |
| US20200122148A1 (en) * | 2018-10-22 | 2020-04-23 | Saudi Arabian Oil Company | Precision cuvette holders and supports for optical characterization and measurements |
| US11385154B2 (en) * | 2020-09-28 | 2022-07-12 | Tokyo Electron Limited | Apparatus and method for monitoring and measuring properties of polymers in solutions |
| US11680897B2 (en) * | 2021-02-23 | 2023-06-20 | Joseph R. Demers | Multi-pass spectroscopy apparatus, associated sample holder and methods |
| US11733156B2 (en) | 2021-02-23 | 2023-08-22 | Joseph R. Demers | Semiconductor package for free-space coupling of radiation and method |
| PL247930B1 (pl) * | 2023-08-23 | 2025-09-15 | Univ Warszawski | Uchwyt do mocowania cienkich warstw materiałów w kuwecie pomiarowej do pomiarów fluorymetrycznych |
| AT18456U1 (de) * | 2023-10-19 | 2025-04-15 | Takeda Pharmaceuticals Co | Referenzprobe für Photolumineszenz-Spektrometer |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53105278A (en) * | 1977-02-25 | 1978-09-13 | Nippon Bunko Kogyo Kk | Cell |
| JPS53118587U (enExample) * | 1977-02-25 | 1978-09-20 | ||
| US4153369A (en) * | 1977-11-11 | 1979-05-08 | Farrand Optical Co., Inc. | Convertible dual beam differential spectrofluorometer and absorption meter |
| JPS6236540A (ja) * | 1985-08-09 | 1987-02-17 | Canon Inc | 粒子解析装置 |
| JP3130513B2 (ja) * | 1989-04-19 | 2001-01-31 | イビデン株式会社 | 生体活性物質測定用装置 |
| JPH0367154A (ja) * | 1989-08-04 | 1991-03-22 | Olympus Optical Co Ltd | 側方散乱光測定装置 |
| US5104218A (en) * | 1989-11-09 | 1992-04-14 | General Atomics | Micropipette adaptor for spectrofluorimeters |
| JPH04215043A (ja) * | 1990-12-11 | 1992-08-05 | Sekisui Chem Co Ltd | 酸味物質検知素子 |
| JPH04340444A (ja) * | 1991-05-17 | 1992-11-26 | Sekisui Chem Co Ltd | 苦味物質の検知素子及び検知方法 |
| JP2526454B2 (ja) * | 1991-10-31 | 1996-08-21 | 株式会社島津製作所 | 分光光度計の試料室 |
| JPH0835927A (ja) * | 1994-07-25 | 1996-02-06 | Jasco Corp | 蛍光検出用フローセル及びそれを用いた蛍光検出器 |
| US5710625A (en) * | 1996-04-30 | 1998-01-20 | Hughes Electronics | Spectral oil immersion cell |
| JPH10123055A (ja) * | 1996-10-18 | 1998-05-15 | Hitachi Ltd | 分光蛍光光度計 |
| CN101523191B (zh) * | 2006-10-06 | 2012-06-06 | 株式会社岛津制作所 | 分光光度计 |
| GB2455492B (en) * | 2007-10-08 | 2011-11-23 | Univ Greenwich | Apparatus and method for detection and measurement of target compounds such as food toxins |
| EA017587B1 (ru) * | 2007-11-02 | 2013-01-30 | Медпласт С.А. | Способ и аппарат для мониторинга пространственного образования фибринового сгустка |
| RU79181U1 (ru) * | 2008-07-28 | 2008-12-20 | Федеральное государственное унитарное предприятие Научно-исследовательский институт электромеханики (ФГУП НИИЭМ) | Флуоресцентный сигнализатор масла в сжатых газах |
| US8318506B2 (en) * | 2009-02-27 | 2012-11-27 | The University Of Connecticut | Dialysis adapter cell and method of release testing of a disperse dosage form |
| CN102072880A (zh) * | 2010-12-14 | 2011-05-25 | 北京清博华科技有限公司 | 一种光纤接口的大流量制备流通池 |
| US9279746B2 (en) * | 2012-02-16 | 2016-03-08 | Endress+ Hauser Conducta Inc. | Inline optical sensor with modular flowcell |
| JP5740510B2 (ja) * | 2014-04-30 | 2015-06-24 | シャープ株式会社 | 化学物質判定装置 |
-
2015
- 2015-11-13 JP JP2017544848A patent/JP6659713B2/ja not_active Expired - Fee Related
- 2015-11-13 CA CA2967811A patent/CA2967811A1/en not_active Abandoned
- 2015-11-13 WO PCT/US2015/060658 patent/WO2016077741A1/en not_active Ceased
- 2015-11-13 EP EP15858999.4A patent/EP3218680A4/en not_active Withdrawn
-
2017
- 2017-05-15 US US15/595,342 patent/US10139344B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP3218680A4 (en) | 2018-06-27 |
| US10139344B2 (en) | 2018-11-27 |
| JP2017534890A (ja) | 2017-11-24 |
| US20170315057A1 (en) | 2017-11-02 |
| WO2016077741A1 (en) | 2016-05-19 |
| JP6659713B2 (ja) | 2020-03-04 |
| EP3218680A1 (en) | 2017-09-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20220204 |
|
| FZDE | Discontinued |
Effective date: 20220204 |