CA2948421C - Co-cultivation of propionibacterium and yeast - Google Patents
Co-cultivation of propionibacterium and yeast Download PDFInfo
- Publication number
- CA2948421C CA2948421C CA2948421A CA2948421A CA2948421C CA 2948421 C CA2948421 C CA 2948421C CA 2948421 A CA2948421 A CA 2948421A CA 2948421 A CA2948421 A CA 2948421A CA 2948421 C CA2948421 C CA 2948421C
- Authority
- CA
- Canada
- Prior art keywords
- propionibacterium
- cultivation
- yeast
- medium
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000186429 Propionibacterium Species 0.000 title claims description 90
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 60
- 238000000034 method Methods 0.000 claims abstract description 56
- 238000000855 fermentation Methods 0.000 claims abstract description 50
- 230000004151 fermentation Effects 0.000 claims abstract description 50
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 29
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 69
- 239000006228 supernatant Substances 0.000 claims description 57
- 230000005526 G1 to G0 transition Effects 0.000 claims description 46
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 46
- 239000005862 Whey Substances 0.000 claims description 36
- 102000007544 Whey Proteins Human genes 0.000 claims description 36
- 108010046377 Whey Proteins Proteins 0.000 claims description 36
- 238000005273 aeration Methods 0.000 claims description 29
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 29
- 230000012010 growth Effects 0.000 claims description 26
- 235000019260 propionic acid Nutrition 0.000 claims description 25
- 229940041514 candida albicans extract Drugs 0.000 claims description 24
- 239000012138 yeast extract Substances 0.000 claims description 24
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims description 23
- 239000000047 product Substances 0.000 claims description 21
- 229930003779 Vitamin B12 Natural products 0.000 claims description 17
- 235000011054 acetic acid Nutrition 0.000 claims description 17
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 17
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- 239000008101 lactose Substances 0.000 claims description 15
- 239000003471 mutagenic agent Substances 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 241000186428 Propionibacterium freudenreichii Species 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000002028 Biomass Substances 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 238000013019 agitation Methods 0.000 claims description 7
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 7
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- 239000000843 powder Substances 0.000 claims description 6
- NGSFWBMYFKHRBD-UHFFFAOYSA-M sodium lactate Chemical compound [Na+].CC(O)C([O-])=O NGSFWBMYFKHRBD-UHFFFAOYSA-M 0.000 claims description 6
- 239000001540 sodium lactate Substances 0.000 claims description 6
- 235000011088 sodium lactate Nutrition 0.000 claims description 6
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- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 235000010216 calcium carbonate Nutrition 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 235000011149 sulphuric acid Nutrition 0.000 claims description 4
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 3
- 241000235649 Kluyveromyces Species 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 3
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims 2
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims 1
- 241000159512 Geotrichum Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 61
- 230000002538 fungal effect Effects 0.000 abstract description 61
- 239000000126 substance Substances 0.000 abstract description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 7
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- 229910052760 oxygen Inorganic materials 0.000 abstract description 7
- 239000002699 waste material Substances 0.000 abstract description 5
- 239000002609 medium Substances 0.000 description 57
- 239000002054 inoculum Substances 0.000 description 21
- 241001521757 Propionibacterium sp. Species 0.000 description 15
- 244000005700 microbiome Species 0.000 description 13
- 238000012360 testing method Methods 0.000 description 13
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- 239000001963 growth medium Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 235000013351 cheese Nutrition 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
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- 235000018102 proteins Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 4
- 238000003501 co-culture Methods 0.000 description 4
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- LJUQGASMPRMWIW-UHFFFAOYSA-N 5,6-dimethylbenzimidazole Chemical compound C1=C(C)C(C)=CC2=C1NC=N2 LJUQGASMPRMWIW-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- -1 hydroxyl radicals Chemical class 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
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- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
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- 238000004659 sterilization and disinfection Methods 0.000 description 3
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- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
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- 241001465754 Metazoa Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 150000001243 acetic acids Chemical class 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- 238000011138 biotechnological process Methods 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- RMRCNWBMXRMIRW-BYFNXCQMSA-M cyanocobalamin Chemical compound N#C[Co+]N([C@]1([H])[C@H](CC(N)=O)[C@]\2(CCC(=O)NC[C@H](C)OP(O)(=O)OC3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)C)C/2=C(C)\C([C@H](C/2(C)C)CCC(N)=O)=N\C\2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O RMRCNWBMXRMIRW-BYFNXCQMSA-M 0.000 description 2
- 238000011143 downstream manufacturing Methods 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 229940014662 pantothenate Drugs 0.000 description 2
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- CZIHNRWJTSTCEX-UHFFFAOYSA-N 2 Acetylaminofluorene Chemical compound C1=CC=C2C3=CC=C(NC(=O)C)C=C3CC2=C1 CZIHNRWJTSTCEX-UHFFFAOYSA-N 0.000 description 1
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
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- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14167774.0A EP2942397A1 (en) | 2014-05-09 | 2014-05-09 | Co-cultivation of propionibacterium and yeast |
| EP14167774.0 | 2014-05-09 | ||
| PCT/EP2015/060270 WO2015169967A1 (en) | 2014-05-09 | 2015-05-08 | Co-cultivation of propionibacterium and yeast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2948421A1 CA2948421A1 (en) | 2015-11-12 |
| CA2948421C true CA2948421C (en) | 2024-03-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2948421A Active CA2948421C (en) | 2014-05-09 | 2015-05-08 | Co-cultivation of propionibacterium and yeast |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9938554B2 (enExample) |
| EP (2) | EP2942397A1 (enExample) |
| JP (1) | JP6814131B2 (enExample) |
| KR (1) | KR102265802B1 (enExample) |
| CN (1) | CN106536715B (enExample) |
| AU (1) | AU2015257590B2 (enExample) |
| BR (1) | BR112016025970B1 (enExample) |
| CA (1) | CA2948421C (enExample) |
| DK (1) | DK3140416T3 (enExample) |
| EA (1) | EA037230B1 (enExample) |
| ES (1) | ES2698529T3 (enExample) |
| HU (1) | HUE040087T2 (enExample) |
| MX (1) | MX378401B (enExample) |
| PL (1) | PL3140416T3 (enExample) |
| PT (1) | PT3140416T (enExample) |
| SI (1) | SI3140416T1 (enExample) |
| WO (1) | WO2015169967A1 (enExample) |
| ZA (1) | ZA201608104B (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102017212564A1 (de) * | 2017-07-21 | 2019-01-24 | Martin Frettlöh | Sequentielles Co-Kultivierungsverfahren zur Herstellung eines vitamin- und proteinreichen Nahrungsmittels |
| CA3104259A1 (en) | 2018-06-18 | 2019-12-26 | S&P Ingredient Development, Llc | Isolation of microbial cell lines for organic acid production |
| JP2023513799A (ja) * | 2020-02-14 | 2023-04-03 | エスアンドピー・イングリーディエント・ディベロップメント・エルエルシー | 有機酸産生を最大化するための微生物細胞株の使用 |
| EP4449878A1 (en) * | 2023-04-18 | 2024-10-23 | Purac Biochem B.V. | Liquid propionate-containing fermentate and process for the manufacture thereof |
| EP4574984A1 (en) | 2023-12-21 | 2025-06-25 | Tirlán Limited | A method of producing organic acids or salts thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52154596A (en) * | 1976-06-14 | 1977-12-22 | Vysoka Skola Chem Tech | Controlling method of protein concentrate |
| US5096718A (en) | 1982-09-17 | 1992-03-17 | The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Preserving foods using metabolites of propionibacteria other than propionic acid |
| DK0668359T3 (da) * | 1994-02-22 | 2000-08-07 | Biotechnolog Forschung Gmbh | Fremgangsmåde til kontinuerlig fermentering, som er nyttig ved samtidig optimal fremstilling af propionsyre og B12 vitamin |
| JP3017456B2 (ja) * | 1997-05-08 | 2000-03-06 | 明治乳業株式会社 | プロピオン酸菌の高濃度培養方法及び培養物ならびにその加工物 |
| HUP0204186A3 (en) * | 1998-12-18 | 2004-07-28 | Dsm Ip Assets Bv | Improved process for the production of vitamin b12 |
| EP2614718B1 (en) | 2006-09-08 | 2018-01-10 | CSK Food Enrichment B.V. | Use of yeast and bacteria for making dairy products with improved flavour and/or texture quality characteristics |
| CN100475951C (zh) | 2007-03-13 | 2009-04-08 | 南京工业大学 | 利用费氏丙酸杆菌nx-4制备丙酸及联产维生素b12的方法 |
| KR20170059011A (ko) * | 2007-11-27 | 2017-05-29 | 가부시키가이샤 메이지 | 유산균의 생잔성 향상제 및 생잔성 향상 방법, 및 식품 조성물 |
| CH700192B1 (de) * | 2009-01-12 | 2010-07-15 | Bioforce Ag Roggwil Tg | Verfahren zur Herstellung einer Fermentationsbrühe. |
| EP2569415A4 (en) | 2010-05-14 | 2015-04-22 | Lactoscience Inc | PROCESS FOR FERMENTATION OF A MIXED CULTURE SUBSTRATE FOR PRODUCING ANIMAL-REPRODUCIBLE BIOMASS AND / OR READY FOR MANUAL CONSUMPTION |
-
2014
- 2014-05-09 EP EP14167774.0A patent/EP2942397A1/en not_active Withdrawn
-
2015
- 2015-05-08 WO PCT/EP2015/060270 patent/WO2015169967A1/en not_active Ceased
- 2015-05-08 PT PT15723471T patent/PT3140416T/pt unknown
- 2015-05-08 ES ES15723471T patent/ES2698529T3/es active Active
- 2015-05-08 EP EP15723471.7A patent/EP3140416B1/en active Active
- 2015-05-08 SI SI201530454T patent/SI3140416T1/sl unknown
- 2015-05-08 BR BR112016025970-0A patent/BR112016025970B1/pt active IP Right Grant
- 2015-05-08 US US15/309,869 patent/US9938554B2/en active Active
- 2015-05-08 HU HUE15723471A patent/HUE040087T2/hu unknown
- 2015-05-08 JP JP2017510771A patent/JP6814131B2/ja active Active
- 2015-05-08 CN CN201580024641.3A patent/CN106536715B/zh active Active
- 2015-05-08 DK DK15723471.7T patent/DK3140416T3/en active
- 2015-05-08 AU AU2015257590A patent/AU2015257590B2/en active Active
- 2015-05-08 KR KR1020167034601A patent/KR102265802B1/ko active Active
- 2015-05-08 MX MX2016014718A patent/MX378401B/es unknown
- 2015-05-08 CA CA2948421A patent/CA2948421C/en active Active
- 2015-05-08 EA EA201692263A patent/EA037230B1/ru not_active IP Right Cessation
- 2015-05-08 PL PL15723471T patent/PL3140416T3/pl unknown
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2016
- 2016-11-23 ZA ZA2016/08104A patent/ZA201608104B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EA037230B1 (ru) | 2021-02-24 |
| SI3140416T1 (sl) | 2018-12-31 |
| ES2698529T3 (es) | 2019-02-05 |
| WO2015169967A1 (en) | 2015-11-12 |
| MX378401B (es) | 2025-03-10 |
| BR112016025970B1 (pt) | 2022-11-01 |
| ZA201608104B (en) | 2019-04-24 |
| JP6814131B2 (ja) | 2021-01-13 |
| CN106536715A (zh) | 2017-03-22 |
| KR102265802B1 (ko) | 2021-06-16 |
| AU2015257590A1 (en) | 2016-11-10 |
| AU2015257590B2 (en) | 2019-03-14 |
| US9938554B2 (en) | 2018-04-10 |
| US20170145467A1 (en) | 2017-05-25 |
| CN106536715B (zh) | 2018-12-21 |
| PL3140416T3 (pl) | 2019-03-29 |
| JP2017514526A (ja) | 2017-06-08 |
| PT3140416T (pt) | 2018-11-16 |
| EP3140416A1 (en) | 2017-03-15 |
| MX2016014718A (es) | 2017-03-06 |
| KR20170002620A (ko) | 2017-01-06 |
| CA2948421A1 (en) | 2015-11-12 |
| BR112016025970A2 (enExample) | 2017-08-15 |
| EP3140416B1 (en) | 2018-08-01 |
| EA201692263A1 (ru) | 2017-04-28 |
| EP2942397A1 (en) | 2015-11-11 |
| DK3140416T3 (en) | 2018-11-26 |
| HUE040087T2 (hu) | 2019-02-28 |
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