CA2945548C - Method of preparing cells for 3d tissue culture - Google Patents

Method of preparing cells for 3d tissue culture Download PDF

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Publication number
CA2945548C
CA2945548C CA2945548A CA2945548A CA2945548C CA 2945548 C CA2945548 C CA 2945548C CA 2945548 A CA2945548 A CA 2945548A CA 2945548 A CA2945548 A CA 2945548A CA 2945548 C CA2945548 C CA 2945548C
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Prior art keywords
cells
cndot
tissue culture
cell
tissue
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CA2945548A
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English (en)
French (fr)
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CA2945548A1 (en
Inventor
Simon MESSNER
Wolfgang Moritz
Jan Lichtenberg
Jens M. KELM
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INSPHERO AG
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INSPHERO AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062General methods for three-dimensional culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0671Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067Liver cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2945548A 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture Active CA2945548C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1406716.9 2014-04-15
GBGB1406716.9A GB201406716D0 (en) 2014-04-15 2014-04-15 Method of preparing cells for 3D tissue culture
PCT/EP2015/058174 WO2015158777A1 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture

Publications (2)

Publication Number Publication Date
CA2945548A1 CA2945548A1 (en) 2015-10-22
CA2945548C true CA2945548C (en) 2019-11-12

Family

ID=50844988

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2945548A Active CA2945548C (en) 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture

Country Status (10)

Country Link
US (2) US20170037364A1 (enExample)
EP (1) EP3132023B1 (enExample)
JP (1) JP6492106B2 (enExample)
CN (1) CN106459895B (enExample)
AU (1) AU2015248875B9 (enExample)
CA (1) CA2945548C (enExample)
DK (1) DK3132023T3 (enExample)
GB (1) GB201406716D0 (enExample)
SG (1) SG11201608463WA (enExample)
WO (1) WO2015158777A1 (enExample)

Families Citing this family (10)

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JP6758026B2 (ja) * 2015-04-17 2020-09-23 株式会社日立ハイテク 成分分析装置、薬剤成分分析装置、成分分析方法及び薬剤成分分析方法
WO2017117333A1 (en) * 2015-12-30 2017-07-06 Cellular Dynamics International, Inc. Microtissue formation using stem cell-derived human hepatocytes
GB201609663D0 (en) 2016-06-02 2016-07-20 Stemtek Therapeutics Sl Methods for producing cancer stem cell spheroids
US20210130790A1 (en) * 2017-03-10 2021-05-06 Merck Patent Gmbh Infected Cell Cultures
CN109294971A (zh) * 2018-09-29 2019-02-01 西安交通大学 一种用于药物筛选的颈动脉分叉三维细胞培养模型及其构建方法
DE102019216886A1 (de) 2019-10-31 2021-05-06 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Transfer zumindest eines partikels in einer flüssigkeit von einer abgabeeinheit zu einer empfangseinheit
CN111471641B (zh) * 2020-02-03 2021-11-05 东华大学 多片层单元水凝胶包被的仿生毛细血管网的3d打印制法
CN111150885A (zh) * 2020-03-05 2020-05-15 陕西佰傲干细胞再生医学有限公司 软骨微囊及其制备方法和应用
US12076437B2 (en) 2021-07-12 2024-09-03 Brown University Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue
WO2025073379A1 (en) 2023-10-06 2025-04-10 Insphero Ag Device for studying interactions of biological specimen

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
WO2003055487A1 (en) * 2002-01-04 2003-07-10 The University Of Adelaide METHOD OF CONTROLLING DAMAGE MEDIATED BY α, β- UNSATURATED ALDEHYDES
WO2003084468A2 (en) * 2002-04-03 2003-10-16 Artecel Sciences, Inc. Improvements of adipocytic differentiated adipose derived adult stem cells and uses thereof
JP2004254674A (ja) * 2003-02-25 2004-09-16 Mutsumi Takagi 動物細胞の分化誘導培養方法
WO2006032092A1 (en) * 2004-09-24 2006-03-30 Angioblast Systems, Inc. Multipotential expanded mesenchymal precursor cell progeny (memp) and uses thereof
WO2007124023A2 (en) * 2006-04-21 2007-11-01 Wake Forest University Health Sciences Ink-jet printing of tissues
CA2667359C (en) * 2006-10-23 2014-07-22 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
CA2677679A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
WO2010059828A1 (en) * 2008-11-19 2010-05-27 Anthrogenesis Corporation Amnion derived adherent cells
US20110250688A1 (en) * 2008-11-24 2011-10-13 Immunotrex Corporation Three Dimensional Tissue Generation
ES2609006T3 (es) * 2009-10-16 2017-04-18 Rutgers, The State University Of New Jersey Procedimeinto de tratamiento de lesión crónica de tejido nervioso usando una estrategia de terapia celular
IL288647B2 (en) * 2009-11-30 2023-12-01 Pluri Biotech Ltd Adherent Cells From Placenta And Use Of Same In Treating Heart Failure
US20110229970A1 (en) * 2010-03-05 2011-09-22 Florida State University Research Foundation Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use
EP2580319B1 (en) * 2010-06-11 2016-07-20 Takara Bio Europe AB 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes
CN103379921A (zh) * 2010-12-17 2013-10-30 人类起源公司 使用羊膜来源的贴壁细胞治疗脊髓损伤和外伤性脑损伤
AU2012308715A1 (en) * 2011-09-12 2014-04-24 Organovo, Inc. Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same

Also Published As

Publication number Publication date
SG11201608463WA (en) 2016-11-29
EP3132023A1 (en) 2017-02-22
CN106459895B (zh) 2021-06-01
CN106459895A (zh) 2017-02-22
JP6492106B2 (ja) 2019-03-27
US20170037364A1 (en) 2017-02-09
AU2015248875A1 (en) 2016-10-27
JP2017511146A (ja) 2017-04-20
WO2015158777A1 (en) 2015-10-22
EP3132023B1 (en) 2021-06-02
AU2015248875B9 (en) 2019-05-23
GB201406716D0 (en) 2014-05-28
CA2945548A1 (en) 2015-10-22
AU2015248875B2 (en) 2019-04-04
US20230235277A1 (en) 2023-07-27
DK3132023T3 (da) 2021-08-09

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