AU2015248875B9 - Method of preparing cells for 3D tissue culture - Google Patents

Method of preparing cells for 3D tissue culture Download PDF

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Publication number
AU2015248875B9
AU2015248875B9 AU2015248875A AU2015248875A AU2015248875B9 AU 2015248875 B9 AU2015248875 B9 AU 2015248875B9 AU 2015248875 A AU2015248875 A AU 2015248875A AU 2015248875 A AU2015248875 A AU 2015248875A AU 2015248875 B9 AU2015248875 B9 AU 2015248875B9
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AU
Australia
Prior art keywords
cells
tissue culture
cell
tissue
culture
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Ceased
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AU2015248875A
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English (en)
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AU2015248875B2 (en
AU2015248875A1 (en
Inventor
Jens M. KELM
Jan Lichtenberg
Simon MESSNER
Wolfgang Moritz
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INSPHERO AG
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INSPHERO AG
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Publication of AU2015248875B2 publication Critical patent/AU2015248875B2/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062General methods for three-dimensional culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0671Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067Liver cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2015248875A 2014-04-15 2015-04-15 Method of preparing cells for 3D tissue culture Ceased AU2015248875B9 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1406716.9A GB201406716D0 (en) 2014-04-15 2014-04-15 Method of preparing cells for 3D tissue culture
GB1406716.9 2014-04-15
PCT/EP2015/058174 WO2015158777A1 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture

Publications (3)

Publication Number Publication Date
AU2015248875A1 AU2015248875A1 (en) 2016-10-27
AU2015248875B2 AU2015248875B2 (en) 2019-04-04
AU2015248875B9 true AU2015248875B9 (en) 2019-05-23

Family

ID=50844988

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2015248875A Ceased AU2015248875B9 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3D tissue culture

Country Status (10)

Country Link
US (2) US20170037364A1 (enExample)
EP (1) EP3132023B1 (enExample)
JP (1) JP6492106B2 (enExample)
CN (1) CN106459895B (enExample)
AU (1) AU2015248875B9 (enExample)
CA (1) CA2945548C (enExample)
DK (1) DK3132023T3 (enExample)
GB (1) GB201406716D0 (enExample)
SG (1) SG11201608463WA (enExample)
WO (1) WO2015158777A1 (enExample)

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Publication number Priority date Publication date Assignee Title
JP6758026B2 (ja) * 2015-04-17 2020-09-23 株式会社日立ハイテク 成分分析装置、薬剤成分分析装置、成分分析方法及び薬剤成分分析方法
WO2017117333A1 (en) * 2015-12-30 2017-07-06 Cellular Dynamics International, Inc. Microtissue formation using stem cell-derived human hepatocytes
GB201609663D0 (en) 2016-06-02 2016-07-20 Stemtek Therapeutics Sl Methods for producing cancer stem cell spheroids
CN110431225B (zh) * 2017-03-10 2024-04-12 默克专利股份公司 受感染的细胞培养物
CN109294971A (zh) * 2018-09-29 2019-02-01 西安交通大学 一种用于药物筛选的颈动脉分叉三维细胞培养模型及其构建方法
DE102019216886A1 (de) 2019-10-31 2021-05-06 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Transfer zumindest eines partikels in einer flüssigkeit von einer abgabeeinheit zu einer empfangseinheit
CN111471641B (zh) * 2020-02-03 2021-11-05 东华大学 多片层单元水凝胶包被的仿生毛细血管网的3d打印制法
CN111150885A (zh) * 2020-03-05 2020-05-15 陕西佰傲干细胞再生医学有限公司 软骨微囊及其制备方法和应用
US12076437B2 (en) 2021-07-12 2024-09-03 Brown University Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue
WO2025073379A1 (en) 2023-10-06 2025-04-10 Insphero Ag Device for studying interactions of biological specimen

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008100497A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
WO2011154552A1 (en) * 2010-06-11 2011-12-15 Cellartis Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes

Family Cites Families (14)

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FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
WO2003055487A1 (en) * 2002-01-04 2003-07-10 The University Of Adelaide METHOD OF CONTROLLING DAMAGE MEDIATED BY α, β- UNSATURATED ALDEHYDES
EP1496978A4 (en) * 2002-04-03 2006-11-29 Artecel Sciences Inc IMPROVEMENTS IN DIFFERENTIATED ADULT DIFFERENTIATED STEM CELLS DERIVED FROM ADIPOSE TISSUE AND USES THEREOF
JP2004254674A (ja) * 2003-02-25 2004-09-16 Mutsumi Takagi 動物細胞の分化誘導培養方法
US20080260694A1 (en) * 2004-09-24 2008-10-23 Angioblast Systems, Inc. Multipotential Expanded Mesenchymal Precursor Cell Progeny (Memp) and Uses Thereof
US20090208466A1 (en) * 2006-04-21 2009-08-20 James Yoo Ink-jet printing of tissues
CA2850793A1 (en) * 2006-10-23 2008-05-02 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
CA2743566C (en) * 2008-11-19 2021-11-09 Anthrogenesis Corporation Amnion derived adherent cells
US20110250688A1 (en) * 2008-11-24 2011-10-13 Immunotrex Corporation Three Dimensional Tissue Generation
WO2011046570A1 (en) * 2009-10-16 2011-04-21 The University Of Medicine And Dentistry Of New Jersey Method for treating chronic nerve tissue injury using a cell therapy strategy
AU2010322808B9 (en) * 2009-11-30 2014-08-07 Pluri Biotech Ltd Adherent cells from placenta and use of same in disease treatment
US20110229970A1 (en) * 2010-03-05 2011-09-22 Florida State University Research Foundation Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use
AU2011343738A1 (en) * 2010-12-17 2013-07-04 Anthrogenesis Corporation Treatment of spinal cord injury and traumatic brain injury using amnion derived adherent cells
BR112014005746A2 (pt) * 2011-09-12 2017-03-28 Organovo Inc tecidos engendrados para usos em pesquisa in vitro, arranjos destes, e métodos de fabricar os mesmos

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008100497A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
WO2011154552A1 (en) * 2010-06-11 2011-12-15 Cellartis Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes

Also Published As

Publication number Publication date
JP2017511146A (ja) 2017-04-20
WO2015158777A1 (en) 2015-10-22
AU2015248875B2 (en) 2019-04-04
GB201406716D0 (en) 2014-05-28
DK3132023T3 (da) 2021-08-09
JP6492106B2 (ja) 2019-03-27
CA2945548A1 (en) 2015-10-22
EP3132023B1 (en) 2021-06-02
US20230235277A1 (en) 2023-07-27
AU2015248875A1 (en) 2016-10-27
CA2945548C (en) 2019-11-12
CN106459895B (zh) 2021-06-01
US20170037364A1 (en) 2017-02-09
CN106459895A (zh) 2017-02-22
SG11201608463WA (en) 2016-11-29
EP3132023A1 (en) 2017-02-22

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