CA2938213C - Improved methods for processing dna substrates - Google Patents

Improved methods for processing dna substrates Download PDF

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Publication number
CA2938213C
CA2938213C CA2938213A CA2938213A CA2938213C CA 2938213 C CA2938213 C CA 2938213C CA 2938213 A CA2938213 A CA 2938213A CA 2938213 A CA2938213 A CA 2938213A CA 2938213 C CA2938213 C CA 2938213C
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Prior art keywords
oligonucleotide
adapter
dna
ligation
ligase
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French (fr)
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CA2938213A1 (en
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Vladimir Makarov
Julie LALIBERTE
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Integrated DNA Technologies Inc
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Integrated DNA Technologies Inc
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Priority to CA3211550A priority Critical patent/CA3211550A1/en
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2521/525—Phosphatase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CA2938213A 2014-01-31 2015-01-30 Improved methods for processing dna substrates Active CA2938213C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3211550A CA3211550A1 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461934515P 2014-01-31 2014-01-31
US61/934,515 2014-01-31
US201462078313P 2014-11-11 2014-11-11
US201462078309P 2014-11-11 2014-11-11
US62/078,309 2014-11-11
US62/078,313 2014-11-11
PCT/US2015/013994 WO2015117040A1 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

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CA2938213A1 CA2938213A1 (en) 2015-08-06
CA2938213C true CA2938213C (en) 2023-10-10

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US (6) US10316357B2 (cg-RX-API-DMAC7.html)
EP (5) EP3604544B1 (cg-RX-API-DMAC7.html)
JP (2) JP6723929B2 (cg-RX-API-DMAC7.html)
KR (2) KR102482592B1 (cg-RX-API-DMAC7.html)
CN (2) CN109971826B (cg-RX-API-DMAC7.html)
AU (1) AU2015210705B2 (cg-RX-API-DMAC7.html)
CA (2) CA3211550A1 (cg-RX-API-DMAC7.html)
DK (1) DK3604544T3 (cg-RX-API-DMAC7.html)
ES (1) ES2908644T3 (cg-RX-API-DMAC7.html)
HU (1) HUE058149T2 (cg-RX-API-DMAC7.html)
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