CA2932943C - High-throughput and highly multiplexed imaging with programmable nucleic acid probes - Google Patents
High-throughput and highly multiplexed imaging with programmable nucleic acid probes Download PDFInfo
- Publication number
- CA2932943C CA2932943C CA2932943A CA2932943A CA2932943C CA 2932943 C CA2932943 C CA 2932943C CA 2932943 A CA2932943 A CA 2932943A CA 2932943 A CA2932943 A CA 2932943A CA 2932943 C CA2932943 C CA 2932943C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acids
- target
- specific binding
- docking
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/601—Detection means characterised by use of a special device being a microscope, e.g. atomic force microscopy [AFM]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461951461P | 2014-03-11 | 2014-03-11 | |
| US61/951,461 | 2014-03-11 | ||
| PCT/US2015/020034 WO2015138653A1 (en) | 2014-03-11 | 2015-03-11 | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2932943A1 CA2932943A1 (en) | 2015-09-17 |
| CA2932943C true CA2932943C (en) | 2023-09-26 |
Family
ID=54072381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2932943A Active CA2932943C (en) | 2014-03-11 | 2015-03-11 | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US10294510B2 (https=) |
| EP (4) | EP4245859A3 (https=) |
| JP (3) | JP6757255B2 (https=) |
| KR (2) | KR102313982B1 (https=) |
| CN (2) | CN110004208A (https=) |
| AU (2) | AU2015229406C1 (https=) |
| BR (1) | BR112016018314A2 (https=) |
| CA (1) | CA2932943C (https=) |
| IL (1) | IL246962B (https=) |
| RU (1) | RU2662969C2 (https=) |
| WO (1) | WO2015138653A1 (https=) |
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| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| AU2014296253A1 (en) | 2013-07-30 | 2016-02-04 | President And Fellows Of Harvard College | Quantitative DNA-based imaging and super-resolution imaging |
| EP4245859A3 (en) | 2014-03-11 | 2023-11-08 | President and Fellows of Harvard College | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| CN112029826B (zh) | 2014-07-30 | 2025-03-14 | 哈佛学院院长及董事 | 用于测定核酸的系统和方法 |
| US20180224461A1 (en) | 2015-08-07 | 2018-08-09 | President And Fellows Of Harvard College | Super resolution imaging of protein-protein interactions |
| EP3448886A4 (en) * | 2016-04-26 | 2019-12-11 | Ultivue, Inc. | SUPER-HIGH-RESOLUTION IMMUNOFLUORESCENCE WITH BINDING-LIMITED PREVIEW |
| WO2017197147A2 (en) * | 2016-05-11 | 2017-11-16 | Atkinson Robert G | Molecular beacon comprising prefabricated components and associated products, processes, and methods of use |
| JP6996502B2 (ja) * | 2016-05-19 | 2022-01-17 | 凸版印刷株式会社 | 標的分子の検出方法 |
| EP3465162A4 (en) * | 2016-06-02 | 2020-03-25 | Ultivue, Inc. | COMPOSITIONS AND METHODS FOR ACCELERATING ANTIBODY EXCHANGE IMAGING |
| CN109789228B (zh) * | 2016-07-27 | 2022-10-21 | 斯坦福大学托管董事会 | 高度复用荧光成像 |
| JPWO2018038076A1 (ja) * | 2016-08-22 | 2019-06-20 | 日産化学株式会社 | Ｅ−カドヘリン結合型核酸アプタマーを用いる細胞足場材料 |
| JP2020504600A (ja) | 2016-12-09 | 2020-02-13 | アルティヴュー， インク． | 標識化核酸イメージング剤を使用した多重イメージングのための改善された方法 |
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| CA3122569A1 (en) | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
| JP2022513739A (ja) * | 2018-12-14 | 2022-02-09 | アルティヴュー， インク． | 標的を逐次検出するための方法及び組成物 |
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| EP3983803A2 (en) * | 2019-06-14 | 2022-04-20 | Akoya Biosciences, Inc. | Multiplexed tissue imaging |
| CN111004622B (zh) * | 2019-11-28 | 2023-08-15 | 郑州轻工业大学 | 一种检测多巴胺的高灵敏荧光探针的制备方法及其应用 |
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| CN112014563B (zh) * | 2020-08-27 | 2022-02-15 | 武汉大学 | 直接检测血液中循环肿瘤细胞的分子信标传递纳米探针、其制备方法及应用 |
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| CN113584133B (zh) * | 2021-08-30 | 2024-03-26 | 中国药科大学 | 基于颜色编码与可编程性荧光探针的多重靶标原位检测方法 |
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| CN116686824A (zh) * | 2023-06-07 | 2023-09-05 | 晶准生物医学（深圳）有限公司 | 血液样本保存液及其制备方法和应用 |
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-
2015
- 2015-03-11 EP EP23176015.8A patent/EP4245859A3/en active Pending
- 2015-03-11 CA CA2932943A patent/CA2932943C/en active Active
- 2015-03-11 CN CN201910186444.1A patent/CN110004208A/zh active Pending
- 2015-03-11 KR KR1020167021588A patent/KR102313982B1/ko active Active
- 2015-03-11 BR BR112016018314A patent/BR112016018314A2/pt not_active Application Discontinuation
- 2015-03-11 WO PCT/US2015/020034 patent/WO2015138653A1/en not_active Ceased
- 2015-03-11 RU RU2016139490A patent/RU2662969C2/ru active
- 2015-03-11 JP JP2016551812A patent/JP6757255B2/ja active Active
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