CA2931518C - Viral vector manufacture - Google Patents
Viral vector manufacture Download PDFInfo
- Publication number
- CA2931518C CA2931518C CA2931518A CA2931518A CA2931518C CA 2931518 C CA2931518 C CA 2931518C CA 2931518 A CA2931518 A CA 2931518A CA 2931518 A CA2931518 A CA 2931518A CA 2931518 C CA2931518 C CA 2931518C
- Authority
- CA
- Canada
- Prior art keywords
- cell
- virus
- cells
- cho
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004519 manufacturing process Methods 0.000 title description 35
- 239000013603 viral vector Substances 0.000 title description 10
- 210000004027 cell Anatomy 0.000 claims abstract description 488
- 210000004962 mammalian cell Anatomy 0.000 claims abstract description 37
- 101150016130 CP77 gene Proteins 0.000 claims abstract description 11
- 101100372716 Escherichia phage 186 fil gene Proteins 0.000 claims abstract 4
- 241000700605 Viruses Species 0.000 claims description 161
- 230000014509 gene expression Effects 0.000 claims description 130
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 107
- 238000000034 method Methods 0.000 claims description 53
- 241000700618 Vaccinia virus Species 0.000 claims description 40
- 239000002773 nucleotide Substances 0.000 claims description 33
- 125000003729 nucleotide group Chemical group 0.000 claims description 31
- 108020004705 Codon Proteins 0.000 claims description 28
- 230000003321 amplification Effects 0.000 claims description 28
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- 230000008569 process Effects 0.000 claims description 19
- 230000001902 propagating effect Effects 0.000 claims description 18
- 101150083809 D10L gene Proteins 0.000 claims description 17
- 101150044623 D13L gene Proteins 0.000 claims description 17
- 101100498120 Vaccinia virus (strain Western Reserve) VACWR118 gene Proteins 0.000 claims description 17
- 241000699802 Cricetulus griseus Species 0.000 claims description 13
- 210000003501 vero cell Anatomy 0.000 claims description 11
- 238000000338 in vitro Methods 0.000 claims description 10
- 210000001672 ovary Anatomy 0.000 claims description 10
- 230000029812 viral genome replication Effects 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 description 228
- 208000015181 infectious disease Diseases 0.000 description 136
- 102000004169 proteins and genes Human genes 0.000 description 127
- 230000003612 virological effect Effects 0.000 description 101
- 239000013598 vector Substances 0.000 description 65
- 150000007523 nucleic acids Chemical class 0.000 description 59
- 108091026890 Coding region Proteins 0.000 description 57
- 206010046865 Vaccinia virus infection Diseases 0.000 description 57
- 208000007089 vaccinia Diseases 0.000 description 51
- 239000002609 medium Substances 0.000 description 49
- 108020004414 DNA Proteins 0.000 description 46
- 241000282414 Homo sapiens Species 0.000 description 39
- 239000001963 growth medium Substances 0.000 description 39
- 239000000284 extract Substances 0.000 description 37
- 108091028043 Nucleic acid sequence Proteins 0.000 description 35
- 238000010790 dilution Methods 0.000 description 35
- 239000012895 dilution Substances 0.000 description 35
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 32
- 102000039446 nucleic acids Human genes 0.000 description 31
- 108020004707 nucleic acids Proteins 0.000 description 31
- 108090000765 processed proteins & peptides Proteins 0.000 description 27
- 239000012528 membrane Substances 0.000 description 24
- 239000000047 product Substances 0.000 description 24
- 230000001105 regulatory effect Effects 0.000 description 24
- 238000010367 cloning Methods 0.000 description 22
- 238000012217 deletion Methods 0.000 description 22
- 230000037430 deletion Effects 0.000 description 22
- 238000004448 titration Methods 0.000 description 22
- 230000006801 homologous recombination Effects 0.000 description 21
- 238000002744 homologous recombination Methods 0.000 description 21
- 230000002458 infectious effect Effects 0.000 description 21
- 125000003275 alpha amino acid group Chemical group 0.000 description 20
- 102000004196 processed proteins & peptides Human genes 0.000 description 20
- 238000003780 insertion Methods 0.000 description 19
- 230000037431 insertion Effects 0.000 description 19
- 102000040430 polynucleotide Human genes 0.000 description 19
- 108091033319 polynucleotide Proteins 0.000 description 19
- 239000002157 polynucleotide Substances 0.000 description 19
- 230000002103 transcriptional effect Effects 0.000 description 19
- 230000001413 cellular effect Effects 0.000 description 18
- 238000010276 construction Methods 0.000 description 18
- 230000007505 plaque formation Effects 0.000 description 18
- 229920001184 polypeptide Polymers 0.000 description 18
- 230000014616 translation Effects 0.000 description 18
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 17
- 108010021843 fluorescent protein 583 Proteins 0.000 description 17
- 210000002845 virion Anatomy 0.000 description 17
- 241000700626 Cowpox virus Species 0.000 description 16
- 239000002054 inoculum Substances 0.000 description 16
- 108020004999 messenger RNA Proteins 0.000 description 16
- 239000013612 plasmid Substances 0.000 description 16
- 238000013519 translation Methods 0.000 description 15
- 238000001262 western blot Methods 0.000 description 14
- 241000283973 Oryctolagus cuniculus Species 0.000 description 13
- 150000001413 amino acids Chemical group 0.000 description 13
- 201000010099 disease Diseases 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 13
- 230000008488 polyadenylation Effects 0.000 description 13
- 239000003623 enhancer Substances 0.000 description 12
- 230000010354 integration Effects 0.000 description 12
- 239000000427 antigen Substances 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 11
- 238000011161 development Methods 0.000 description 11
- 230000018109 developmental process Effects 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 10
- 241000699800 Cricetinae Species 0.000 description 10
- 230000002238 attenuated effect Effects 0.000 description 10
- 230000000295 complement effect Effects 0.000 description 10
- 238000012258 culturing Methods 0.000 description 10
- 238000001514 detection method Methods 0.000 description 10
- 230000004048 modification Effects 0.000 description 10
- 238000012986 modification Methods 0.000 description 10
- 230000003362 replicative effect Effects 0.000 description 10
- 230000002441 reversible effect Effects 0.000 description 10
- 241000894007 species Species 0.000 description 10
- 230000009261 transgenic effect Effects 0.000 description 10
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 9
- 239000013553 cell monolayer Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 238000003306 harvesting Methods 0.000 description 9
- 230000001939 inductive effect Effects 0.000 description 9
- 238000012423 maintenance Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 229960005486 vaccine Drugs 0.000 description 9
- 230000009385 viral infection Effects 0.000 description 9
- 241000700629 Orthopoxvirus Species 0.000 description 8
- 108010076504 Protein Sorting Signals Proteins 0.000 description 8
- 210000004899 c-terminal region Anatomy 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 241000701022 Cytomegalovirus Species 0.000 description 7
- 238000012408 PCR amplification Methods 0.000 description 7
- 241000288906 Primates Species 0.000 description 7
- 208000036142 Viral infection Diseases 0.000 description 7
- 238000007792 addition Methods 0.000 description 7
- 230000003115 biocidal effect Effects 0.000 description 7
- 210000000805 cytoplasm Anatomy 0.000 description 7
- 239000005090 green fluorescent protein Substances 0.000 description 7
- 230000028993 immune response Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 238000010222 PCR analysis Methods 0.000 description 6
- 239000012980 RPMI-1640 medium Substances 0.000 description 6
- 239000006180 TBST buffer Substances 0.000 description 6
- 108020005202 Viral DNA Proteins 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 230000000977 initiatory effect Effects 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 239000002243 precursor Substances 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000001225 therapeutic effect Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 238000010361 transduction Methods 0.000 description 6
- 230000026683 transduction Effects 0.000 description 6
- 241000271566 Aves Species 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 5
- 108090000157 Metallothionein Proteins 0.000 description 5
- 229930193140 Neomycin Natural products 0.000 description 5
- 239000000020 Nitrocellulose Substances 0.000 description 5
- 241000700647 Variola virus Species 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 210000005260 human cell Anatomy 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 5
- 230000002101 lytic effect Effects 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 229960004927 neomycin Drugs 0.000 description 5
- 229920001220 nitrocellulos Polymers 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000000644 propagated effect Effects 0.000 description 5
- 230000000069 prophylactic effect Effects 0.000 description 5
- 238000011321 prophylaxis Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- 230000008685 targeting Effects 0.000 description 5
- 239000012096 transfection reagent Substances 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 230000024540 transposon integration Effects 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 4
- 108700024394 Exon Proteins 0.000 description 4
- 108091006010 FLAG-tagged proteins Proteins 0.000 description 4
- GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 4
- 241000714474 Rous sarcoma virus Species 0.000 description 4
- 108091081024 Start codon Proteins 0.000 description 4
- 108091023040 Transcription factor Proteins 0.000 description 4
- 102000040945 Transcription factor Human genes 0.000 description 4
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 201000003740 cowpox Diseases 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 description 4
- 229940097277 hygromycin b Drugs 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000035800 maturation Effects 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 239000013600 plasmid vector Substances 0.000 description 4
- 108010054624 red fluorescent protein Proteins 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 210000003705 ribosome Anatomy 0.000 description 4
- 238000010257 thawing Methods 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 230000017105 transposition Effects 0.000 description 4
- 238000012795 verification Methods 0.000 description 4
- 238000003260 vortexing Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- 108010032595 Antibody Binding Sites Proteins 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 101710167800 Capsid assembly scaffolding protein Proteins 0.000 description 3
- 241000282693 Cercopithecidae Species 0.000 description 3
- 241000283086 Equidae Species 0.000 description 3
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical group C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 241000270322 Lepidosauria Species 0.000 description 3
- -1 Lett Chemical compound 0.000 description 3
- 102000003792 Metallothionein Human genes 0.000 description 3
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 description 3
- 102000002508 Peptide Elongation Factors Human genes 0.000 description 3
- 108010068204 Peptide Elongation Factors Proteins 0.000 description 3
- 101710130420 Probable capsid assembly scaffolding protein Proteins 0.000 description 3
- 101710204410 Scaffold protein Proteins 0.000 description 3
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 3
- HDOVUKNUBWVHOX-QMMMGPOBSA-N Valacyclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCOC(=O)[C@@H](N)C(C)C)C=N2 HDOVUKNUBWVHOX-QMMMGPOBSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000012160 loading buffer Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- 238000007747 plating Methods 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000002463 transducing effect Effects 0.000 description 3
- 241000701161 unidentified adenovirus Species 0.000 description 3
- YLOCGHYTXIINAI-XKUOMLDTSA-N (2s)-2-amino-3-(4-hydroxyphenyl)propanoic acid;(2s)-2-aminopentanedioic acid;(2s)-2-aminopropanoic acid;(2s)-2,6-diaminohexanoic acid Chemical compound C[C@H](N)C(O)=O.NCCCC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CCC(O)=O.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 YLOCGHYTXIINAI-XKUOMLDTSA-N 0.000 description 2
- OJHZNMVJJKMFGX-RNWHKREASA-N (4r,4ar,7ar,12bs)-9-methoxy-3-methyl-1,2,4,4a,5,6,7a,13-octahydro-4,12-methanobenzofuro[3,2-e]isoquinoline-7-one;2,3-dihydroxybutanedioic acid Chemical compound OC(=O)C(O)C(O)C(O)=O.O=C([C@@H]1O2)CC[C@H]3[C@]4([H])N(C)CC[C@]13C1=C2C(OC)=CC=C1C4 OJHZNMVJJKMFGX-RNWHKREASA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000252073 Anguilliformes Species 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 2
- 241000700663 Avipoxvirus Species 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 241000700198 Cavia Species 0.000 description 2
- 241000283153 Cetacea Species 0.000 description 2
- 101000709520 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) Atypical response regulator protein ChxR Proteins 0.000 description 2
- 102000016726 Coat Protein Complex I Human genes 0.000 description 2
- 108010092897 Coat Protein Complex I Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 101150074155 DHFR gene Proteins 0.000 description 2
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XZWYTXMRWQJBGX-VXBMVYAYSA-N FLAG peptide Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=C(O)C=C1 XZWYTXMRWQJBGX-VXBMVYAYSA-N 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- 102100031483 High mobility group protein 20A Human genes 0.000 description 2
- 241001272567 Hominoidea Species 0.000 description 2
- 101000867036 Homo sapiens High mobility group protein 20A Proteins 0.000 description 2
- 101710128836 Large T antigen Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241000282553 Macaca Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 101710159527 Maturation protein A Proteins 0.000 description 2
- 101710091157 Maturation protein A2 Proteins 0.000 description 2
- 241000700627 Monkeypox virus Species 0.000 description 2
- 241000713333 Mouse mammary tumor virus Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108091092724 Noncoding DNA Proteins 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 241001504519 Papio ursinus Species 0.000 description 2
- 241000700639 Parapoxvirus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 108091034057 RNA (poly(A)) Proteins 0.000 description 2
- 101150085390 RPM1 gene Proteins 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000010459 TALEN Methods 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 241000700622 Vaccinia virus Copenhagen Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 101150087698 alpha gene Proteins 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 238000004422 calculation algorithm Methods 0.000 description 2
- 229910002091 carbon monoxide Inorganic materials 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000007541 cellular toxicity Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 108020001096 dihydrofolate reductase Proteins 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 108091006047 fluorescent proteins Proteins 0.000 description 2
- 102000034287 fluorescent proteins Human genes 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
- 108091006104 gene-regulatory proteins Proteins 0.000 description 2
- 102000034356 gene-regulatory proteins Human genes 0.000 description 2
- 238000010362 genome editing Methods 0.000 description 2
- 229960002518 gentamicin Drugs 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 229940065638 intron a Drugs 0.000 description 2
- 238000000464 low-speed centrifugation Methods 0.000 description 2
- 201000004792 malaria Diseases 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000007634 remodeling Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 229940083538 smallpox vaccine Drugs 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 230000007502 viral entry Effects 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- MUSGYEMSJUFFHT-UWABRSFTSA-N 2-[(4R,7S,10S,13S,19S,22S,25S,28S,31S,34R)-34-[[(2S,3S)-2-[[(2R)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylpentanoyl]amino]-4-[[(2S,3S)-1-amino-3-methyl-1-oxopentan-2-yl]-methylcarbamoyl]-25-(3-amino-3-oxopropyl)-7-(3-carbamimidamidopropyl)-10-(1H-imidazol-5-ylmethyl)-19-(1H-indol-3-ylmethyl)-13,17-dimethyl-28-[(1-methylindol-3-yl)methyl]-6,9,12,15,18,21,24,27,30,33-decaoxo-31-propan-2-yl-1,2-dithia-5,8,11,14,17,20,23,26,29,32-decazacyclopentatriacont-22-yl]acetic acid Chemical compound CC[C@H](C)[C@H](NC(=O)[C@H](N)Cc1ccc(O)cc1)C(=O)N[C@H]1CSSC[C@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](Cc2cnc[nH]2)NC(=O)[C@H](C)NC(=O)CN(C)C(=O)[C@H](Cc2c[nH]c3ccccc23)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](Cc2cn(C)c3ccccc23)NC(=O)[C@@H](NC1=O)C(C)C)C(=O)N(C)[C@@H]([C@@H](C)CC)C(N)=O MUSGYEMSJUFFHT-UWABRSFTSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241001522303 Acanthis Species 0.000 description 1
- VJVQKGYHIZPSNS-FXQIFTODSA-N Ala-Ser-Arg Chemical compound C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCN=C(N)N VJVQKGYHIZPSNS-FXQIFTODSA-N 0.000 description 1
- 108010049777 Ankyrins Proteins 0.000 description 1
- 102000008102 Ankyrins Human genes 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- POTCZYQVVNXUIG-BQBZGAKWSA-N Asp-Gly-Pro Chemical compound OC(=O)C[C@H](N)C(=O)NCC(=O)N1CCC[C@H]1C(O)=O POTCZYQVVNXUIG-BQBZGAKWSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 238000006037 Brook Silaketone rearrangement reaction Methods 0.000 description 1
- 241001455646 Buffalopox virus Species 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 108091033409 CRISPR Proteins 0.000 description 1
- 238000010354 CRISPR gene editing Methods 0.000 description 1
- 101100074330 Caenorhabditis elegans lec-8 gene Proteins 0.000 description 1
- 241000070928 Calligonum comosum Species 0.000 description 1
- 241000288951 Callithrix <genus> Species 0.000 description 1
- 241001137864 Camelpox virus Species 0.000 description 1
- 241000282421 Canidae Species 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000824799 Canis lupus dingo Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700664 Capripoxvirus Species 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 241000251556 Chordata Species 0.000 description 1
- 241000700628 Chordopoxvirinae Species 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 101000932768 Conus catus Alpha-conotoxin CIC Proteins 0.000 description 1
- 241001125840 Coryphaenidae Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 101100440908 Cowpox virus (strain Brighton Red) CP77 gene Proteins 0.000 description 1
- 101150108527 D13 gene Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 102100022317 Dihydropteridine reductase Human genes 0.000 description 1
- 241000725630 Ectromelia virus Species 0.000 description 1
- 206010014612 Encephalitis viral Diseases 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 101000823103 Equus caballus Alpha-1-antiproteinase 4 Proteins 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108091006013 HA-tagged proteins Proteins 0.000 description 1
- 108010052512 High Mobility Group Proteins Proteins 0.000 description 1
- 102000018802 High Mobility Group Proteins Human genes 0.000 description 1
- MDCTVRUPVLZSPG-BQBZGAKWSA-N His-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CNC=N1 MDCTVRUPVLZSPG-BQBZGAKWSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000651211 Homo sapiens Transcription factor PU.1 Proteins 0.000 description 1
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
- 241000726306 Irus Species 0.000 description 1
- 208000022120 Jeavons syndrome Diseases 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- 241000283953 Lagomorpha Species 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000713666 Lentivirus Species 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 241001071864 Lethrinus laticaudis Species 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 206010026749 Mania Diseases 0.000 description 1
- 241000721578 Melopsittacus Species 0.000 description 1
- 241000700559 Molluscipoxvirus Species 0.000 description 1
- ONYFNWIHJBLQKE-ZETCQYMHSA-N N(6)-acetimidoyl-L-lysine Chemical compound CC(=N)NCCCC[C@H](N)C(O)=O ONYFNWIHJBLQKE-ZETCQYMHSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 101150050331 PGIC gene Proteins 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- PYOHODCEOHCZBM-RYUDHWBXSA-N Phe-Met Chemical compound CSCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 PYOHODCEOHCZBM-RYUDHWBXSA-N 0.000 description 1
- 208000005585 Poxviridae Infections Diseases 0.000 description 1
- 102000014961 Protein Precursors Human genes 0.000 description 1
- 241001455645 Rabbitpox virus Species 0.000 description 1
- 101000836070 Rattus norvegicus Serine protease inhibitor A3L Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000288961 Saguinus imperator Species 0.000 description 1
- 241000282695 Saimiri Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000287231 Serinus Species 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 208000001203 Smallpox Diseases 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000700565 Swinepox virus Species 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 102100027654 Transcription factor PU.1 Human genes 0.000 description 1
- 102000006612 Transducin Human genes 0.000 description 1
- 108010087042 Transducin Proteins 0.000 description 1
- 101150046432 Tril gene Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- LUMQYLVYUIRHHU-YJRXYDGGSA-N Tyr-Ser-Thr Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LUMQYLVYUIRHHU-YJRXYDGGSA-N 0.000 description 1
- FZADUTOCSFDBRV-RNXOBYDBSA-N Tyr-Tyr-Trp Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)C1=CC=C(O)C=C1 FZADUTOCSFDBRV-RNXOBYDBSA-N 0.000 description 1
- GVJUTBOZZBTBIG-AVGNSLFASA-N Val-Lys-Arg Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N GVJUTBOZZBTBIG-AVGNSLFASA-N 0.000 description 1
- 241000870995 Variola Species 0.000 description 1
- 101000803389 Viola hederacea Root cyclotide 1 Proteins 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 241001137865 Volepox virus Species 0.000 description 1
- 101710088929 Voltage-dependent L-type calcium channel subunit alpha-1S Proteins 0.000 description 1
- 241000700574 Yatapoxvirus Species 0.000 description 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 108010062796 arginyllysine Proteins 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 102000012740 beta Adrenergic Receptors Human genes 0.000 description 1
- 108010079452 beta Adrenergic Receptors Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000004671 cell-free system Anatomy 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 101150045735 cinB gene Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000032 diagnostic agent Substances 0.000 description 1
- 229940039227 diagnostic agent Drugs 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012909 foetal bovine serum Substances 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001889 high-resolution electron micrograph Methods 0.000 description 1
- 230000007446 host cell death Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229940126601 medicinal product Drugs 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 229940021993 prophylactic vaccine Drugs 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 229960000160 recombinant therapeutic protein Drugs 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 229940021747 therapeutic vaccine Drugs 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 201000002498 viral encephalitis Diseases 0.000 description 1
- 230000017613 viral reproduction Effects 0.000 description 1
- 230000006490 viral transcription Effects 0.000 description 1
- 229960004854 viral vaccine Drugs 0.000 description 1
- 230000010464 virion assembly Effects 0.000 description 1
- 230000006394 virus-host interaction Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24141—Use of virus, viral particle or viral elements as a vector
- C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24151—Methods of production or purification of viral material
- C12N2710/24152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2013904242 | 2013-11-01 | ||
| AU2013904242A AU2013904242A0 (en) | 2013-11-01 | Viral vector manufacture | |
| AU2014900370A AU2014900370A0 (en) | 2014-02-07 | Orthopox vectors and propagation | |
| AU2014900370 | 2014-02-07 | ||
| PCT/AU2014/050330 WO2015061858A1 (en) | 2013-11-01 | 2014-11-03 | Viral vector manufacture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2931518A1 CA2931518A1 (en) | 2015-05-07 |
| CA2931518C true CA2931518C (en) | 2021-03-09 |
Family
ID=53003022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2931518A Active CA2931518C (en) | 2013-11-01 | 2014-11-03 | Viral vector manufacture |
Country Status (24)
| Country | Link |
|---|---|
| US (2) | US10544397B2 (enExample) |
| EP (2) | EP3375882B1 (enExample) |
| JP (2) | JP6682443B2 (enExample) |
| KR (1) | KR102047030B1 (enExample) |
| CN (1) | CN106255758B (enExample) |
| AU (1) | AU2014344738B2 (enExample) |
| CA (1) | CA2931518C (enExample) |
| CY (1) | CY1121414T1 (enExample) |
| DK (2) | DK3063278T3 (enExample) |
| ES (1) | ES2670034T3 (enExample) |
| HR (1) | HRP20180789T1 (enExample) |
| HU (1) | HUE037718T2 (enExample) |
| LT (1) | LT3063278T (enExample) |
| NO (1) | NO3063278T3 (enExample) |
| NZ (1) | NZ720241A (enExample) |
| PL (1) | PL3063278T3 (enExample) |
| PT (1) | PT3063278T (enExample) |
| RS (1) | RS57290B1 (enExample) |
| RU (1) | RU2658487C2 (enExample) |
| SG (1) | SG11201603919XA (enExample) |
| SI (1) | SI3063278T1 (enExample) |
| SM (1) | SMT201800267T1 (enExample) |
| TR (1) | TR201807128T4 (enExample) |
| WO (1) | WO2015061858A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI728173B (zh) * | 2016-08-19 | 2021-05-21 | 澳大利亞商賽門堤斯有限公司 | 病毒疫苗 |
| JP2018081568A (ja) * | 2016-11-17 | 2018-05-24 | グローリー株式会社 | 紙幣収納装置および紙幣処理機 |
| CA3258751A1 (en) * | 2022-06-30 | 2024-01-04 | Bavarian Nordic As | Mammalian cell line for the production of modified Ankara vaccine virus (MVA) |
| EP4590841A2 (en) * | 2022-09-20 | 2025-07-30 | Aldevron, LLC | Best1 vectors and uses thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2604183B1 (fr) * | 1986-09-23 | 1988-12-30 | Transgene Sa | Sequence d'adn, vecteurs, virus recombinants et procede mettant en oeuvre des virus de la vaccine recombinants capables de se multiplier dans les cellules cho |
| EP0758397B1 (en) * | 1994-04-29 | 2005-06-22 | Baxter Healthcare S.A. | Recombinant poxviruses with foreign polynucleotides in essential regions |
| DE60336653D1 (de) * | 2002-08-07 | 2011-05-19 | Bavarian Nordic As | Vaccinia virus wirtspektrum gene zur erhöhung des titers von avipoxviren |
| FR2884255B1 (fr) * | 2005-04-11 | 2010-11-05 | Vivalis | Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe |
| AU2007280004A1 (en) * | 2006-08-04 | 2008-02-07 | Dublin City University | A method of producing recombinant biological products |
-
2014
- 2014-11-03 NZ NZ72024114A patent/NZ720241A/en unknown
- 2014-11-03 CA CA2931518A patent/CA2931518C/en active Active
- 2014-11-03 AU AU2014344738A patent/AU2014344738B2/en active Active
- 2014-11-03 LT LTEP14858802.3T patent/LT3063278T/lt unknown
- 2014-11-03 SM SM20180267T patent/SMT201800267T1/it unknown
- 2014-11-03 TR TR2018/07128T patent/TR201807128T4/tr unknown
- 2014-11-03 DK DK14858802.3T patent/DK3063278T3/en active
- 2014-11-03 SI SI201430731T patent/SI3063278T1/sl unknown
- 2014-11-03 PL PL14858802T patent/PL3063278T3/pl unknown
- 2014-11-03 CN CN201480071836.9A patent/CN106255758B/zh active Active
- 2014-11-03 WO PCT/AU2014/050330 patent/WO2015061858A1/en not_active Ceased
- 2014-11-03 DK DK18154084.0T patent/DK3375882T3/da active
- 2014-11-03 EP EP18154084.0A patent/EP3375882B1/en active Active
- 2014-11-03 JP JP2016550897A patent/JP6682443B2/ja active Active
- 2014-11-03 RS RS20180588A patent/RS57290B1/sr unknown
- 2014-11-03 SG SG11201603919XA patent/SG11201603919XA/en unknown
- 2014-11-03 EP EP14858802.3A patent/EP3063278B1/en active Active
- 2014-11-03 HU HUE14858802A patent/HUE037718T2/hu unknown
- 2014-11-03 PT PT148588023T patent/PT3063278T/pt unknown
- 2014-11-03 US US15/033,541 patent/US10544397B2/en active Active
- 2014-11-03 ES ES14858802.3T patent/ES2670034T3/es active Active
- 2014-11-03 NO NO14858802A patent/NO3063278T3/no unknown
- 2014-11-03 HR HRP20180789TT patent/HRP20180789T1/hr unknown
- 2014-11-03 RU RU2016121425A patent/RU2658487C2/ru active
- 2014-11-03 KR KR1020167014693A patent/KR102047030B1/ko active Active
-
2018
- 2018-05-22 CY CY20181100533T patent/CY1121414T1/el unknown
-
2019
- 2019-10-18 JP JP2019191435A patent/JP2020039340A/ja active Pending
- 2019-12-11 US US16/711,184 patent/US20200190482A1/en not_active Abandoned
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2019377141B2 (en) | Enhanced systems for cell-mediated oncolytic viral therapy | |
| US20200190482A1 (en) | Viral vector manufacture | |
| EP2488649A1 (en) | Recombinant modified vaccinia ankara (mva) vaccinia virus containing restructured insertion sites | |
| US20210113684A1 (en) | Vector-based attenuated poxvirus vaccines | |
| CN105039268A (zh) | 表达鸭坦布苏病毒e蛋白的重组鸭瘟病毒及其构建方法和应用 | |
| CN116479016B (zh) | 牛副流感病毒3型全长感染性克隆及其构建方法、用途 | |
| HK1260426B (en) | Viral vector manufacture | |
| HK1260426A1 (en) | Viral vector manufacture | |
| CN118240879A (zh) | 骨架质粒、重组质粒以及猴痘病毒颗粒和应用 | |
| HK1223395B (en) | Viral vector manufacture | |
| WO2023066920A1 (en) | Recombinant african swine fever virus and uses thereof | |
| CN102417907B (zh) | 靶向抑制羊痘病毒ORF095基因的siRNA序列 | |
| CN102851293B (zh) | 靶向抑制羊痘病毒ORF095基因的序列siRNA-165 | |
| Busha | A Novel Selection Technology for the Discovery of High-Affinity Human Proteins | |
| CN111206022A (zh) | 一种表达拉沙热病毒空衣壳的重组病毒及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20180423 |