CA2901588A1 - Compositions and methods for biological production of isoprene - Google Patents
Compositions and methods for biological production of isoprene Download PDFInfo
- Publication number
- CA2901588A1 CA2901588A1 CA2901588A CA2901588A CA2901588A1 CA 2901588 A1 CA2901588 A1 CA 2901588A1 CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A1 CA2901588 A1 CA 2901588A1
- Authority
- CA
- Canada
- Prior art keywords
- isoprene
- nucleic acid
- methanotrophic
- exogenous nucleic
- naturally occurring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C11/00—Aliphatic unsaturated hydrocarbons
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- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03027—Isoprene synthase (4.2.3.27)
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- General Chemical & Material Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
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| US201361774342P | 2013-03-07 | 2013-03-07 | |
| US61/774,342 | 2013-03-07 | ||
| US201461928333P | 2014-01-16 | 2014-01-16 | |
| US61/928,333 | 2014-01-16 | ||
| PCT/US2014/021258 WO2014138419A1 (en) | 2013-03-07 | 2014-03-06 | Compositions and methods for biological production of isoprene |
Publications (1)
| Publication Number | Publication Date |
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| CA2901588A1 true CA2901588A1 (en) | 2014-09-12 |
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| CA2901588A Abandoned CA2901588A1 (en) | 2013-03-07 | 2014-03-06 | Compositions and methods for biological production of isoprene |
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| Country | Link |
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| US (1) | US20160017374A1 (enExample) |
| EP (1) | EP2964748A4 (enExample) |
| JP (1) | JP2016508737A (enExample) |
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| AU (1) | AU2014225631A1 (enExample) |
| BR (1) | BR112015021003A2 (enExample) |
| CA (1) | CA2901588A1 (enExample) |
| RU (1) | RU2015131480A (enExample) |
| WO (1) | WO2014138419A1 (enExample) |
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| US9695447B2 (en) * | 2012-12-27 | 2017-07-04 | Sekisui Chemical Co., Ltd. | Recombinant cell and method for producing isoprene |
| CA2914641A1 (en) | 2013-06-18 | 2014-12-24 | Calysta, Inc. | Compositions and methods for biological production of lactate from c1 compounds using lactate dehydrogenase transformants |
| JP2017504329A (ja) | 2014-01-16 | 2017-02-09 | キャリスタ, インコーポレイテッド | アミノ酸の増強された産生のための微生物および関連する方法 |
| DK3158065T3 (en) | 2014-06-18 | 2019-04-08 | Calysta Inc | NUCLEIC ACIDS AND VECTORS FOR USE WITH METHANOTROPHIC BACTERIES |
| JP2016059313A (ja) * | 2014-09-17 | 2016-04-25 | 積水化学工業株式会社 | 組換え細胞、並びに、イソプレン又は環式イソプレノイドの生産方法 |
| DE102015103608A1 (de) * | 2015-03-11 | 2016-09-15 | Basf Se | Verfahren zur mikrobiellen de novo Synthese von Terpenen |
| EP3292208B1 (en) | 2015-05-06 | 2020-08-19 | Trelys, Inc. | Compositions and methods for biological production of methionine |
| EP3294758A1 (en) | 2015-05-13 | 2018-03-21 | Calysta, Inc. | Proline auxotrophs |
| US20170145441A1 (en) * | 2015-08-17 | 2017-05-25 | INVISTA North America S.à.r.l. | Methods, hosts, and reagents related thereto for production of unsaturated pentahydrocarbons, derivatives and intermediates thereof |
| US10533192B2 (en) | 2015-12-22 | 2020-01-14 | Nutech Ventures | Production of isoprene by methane-producing archaea |
| KR102546640B1 (ko) * | 2016-01-26 | 2023-06-22 | 에스케이이노베이션 주식회사 | 재조합 호염성 메탄균주를 이용한 이소프렌의 제조방법 |
| JP2018014960A (ja) * | 2016-07-29 | 2018-02-01 | 横浜ゴム株式会社 | 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド |
| CN109689857A (zh) * | 2016-10-28 | 2019-04-26 | 积水化学工业株式会社 | 重组细胞、以及异戊二烯或萜的生产方法 |
| WO2019006257A1 (en) * | 2017-06-30 | 2019-01-03 | Invista North America .S.A.R.L. | METHODS, SYNTHETIC HOSTS AND REAGENTS FOR HYDROCARBON BIOSYNTHESIS |
| US11634733B2 (en) | 2017-06-30 | 2023-04-25 | Inv Nylon Chemicals Americas, Llc | Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof |
| US11505809B2 (en) | 2017-09-28 | 2022-11-22 | Inv Nylon Chemicals Americas Llc | Organisms and biosynthetic processes for hydrocarbon synthesis |
| US11180707B2 (en) * | 2018-08-03 | 2021-11-23 | Faramaz Fred Faramarzi | System and method for duplicating flammable gas |
| CN109943493B (zh) * | 2019-04-17 | 2021-05-18 | 天津大学 | 实现通用酶催化功能多样性的突变体菌株及其构建方法 |
| JP7754406B2 (ja) * | 2021-03-26 | 2025-10-15 | 三菱ケミカル株式会社 | イソプレン産生能を有する遺伝子改変微生物およびこれを用いたイソプレン製造方法 |
| CN119736218A (zh) * | 2023-09-19 | 2025-04-01 | 西安卓虹超源生物科技有限公司 | 一种萜类化合物的生产及菌株 |
| KR20250113943A (ko) * | 2024-01-18 | 2025-07-28 | 한국생명공학연구원 | 메탄으로부터 이소프렌 생산능을 갖는 형질전환체 |
Family Cites Families (9)
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| US6818424B2 (en) * | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
| ATE384119T1 (de) * | 2000-09-29 | 2008-02-15 | Cargill Inc | Isoprenoidproduktion |
| US7947478B2 (en) * | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| BRPI0823506A8 (pt) * | 2007-12-13 | 2018-05-15 | Danisco Us Inc Genencor Div | composições e métodos para a produção de isoprene |
| CA2737223A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| TW201412988A (zh) * | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用dxp及mva途徑之改良之異戊二烯製造 |
| DE102012012913A1 (de) * | 2012-06-27 | 2014-01-02 | Beatrice Saier | Vorrichtung und Verfahren zur schaltbaren Förderung wenigstens eines Mediums |
| BR112015014843A2 (pt) * | 2012-12-21 | 2017-10-10 | Danisco Us Inc | produção de isopreno, isoprenoide, e precursores de isoprenoide usando uma via de mevalonato inferior alternativa |
| US9695447B2 (en) * | 2012-12-27 | 2017-07-04 | Sekisui Chemical Co., Ltd. | Recombinant cell and method for producing isoprene |
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- 2014-03-06 BR BR112015021003A patent/BR112015021003A2/pt not_active IP Right Cessation
- 2014-03-06 US US14/773,118 patent/US20160017374A1/en not_active Abandoned
- 2014-03-06 WO PCT/US2014/021258 patent/WO2014138419A1/en not_active Ceased
- 2014-03-06 AU AU2014225631A patent/AU2014225631A1/en not_active Abandoned
- 2014-03-06 EP EP14760094.4A patent/EP2964748A4/en not_active Withdrawn
- 2014-03-06 RU RU2015131480A patent/RU2015131480A/ru not_active Application Discontinuation
- 2014-03-06 KR KR1020157023131A patent/KR20150125653A/ko not_active Withdrawn
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- 2014-03-06 JP JP2015561662A patent/JP2016508737A/ja active Pending
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| WO2014138419A1 (en) | 2014-09-12 |
| CN105008514A (zh) | 2015-10-28 |
| BR112015021003A2 (pt) | 2017-07-18 |
| JP2016508737A (ja) | 2016-03-24 |
| KR20150125653A (ko) | 2015-11-09 |
| EP2964748A4 (en) | 2016-11-02 |
| EP2964748A1 (en) | 2016-01-13 |
| US20160017374A1 (en) | 2016-01-21 |
| RU2015131480A (ru) | 2017-04-12 |
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