CA2901588A1 - Compositions and methods for biological production of isoprene - Google Patents

Compositions and methods for biological production of isoprene Download PDF

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Publication number
CA2901588A1
CA2901588A1 CA2901588A CA2901588A CA2901588A1 CA 2901588 A1 CA2901588 A1 CA 2901588A1 CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A CA2901588 A CA 2901588A CA 2901588 A1 CA2901588 A1 CA 2901588A1
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CA
Canada
Prior art keywords
isoprene
nucleic acid
methanotrophic
exogenous nucleic
naturally occurring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2901588A
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English (en)
French (fr)
Inventor
Effendi Leonard
Jeremy Minshull
Jon Ness
Thomas Joseph Purcell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Calysta Inc
Original Assignee
Calysta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calysta Inc filed Critical Calysta Inc
Publication of CA2901588A1 publication Critical patent/CA2901588A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C11/00Aliphatic unsaturated hydrocarbons
    • C07C11/02Alkenes
    • C07C11/10Alkenes with five carbon atoms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0093Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/012671-Deoxy-D-xylulose-5-phosphate reductoisomerase (1.1.1.267)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y117/00Oxidoreductases acting on CH or CH2 groups (1.17)
    • C12Y117/01Oxidoreductases acting on CH or CH2 groups (1.17) with NAD+ or NADP+ as acceptor (1.17.1)
    • C12Y117/010024-Hydroxy-3-methylbut-2-enyl diphosphate reductase (1.17.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y117/00Oxidoreductases acting on CH or CH2 groups (1.17)
    • C12Y117/07Oxidoreductases acting on CH or CH2 groups (1.17) with an iron-sulfur protein as acceptor (1.17.7)
    • C12Y117/07001(E)-4-Hydroxy-3-methylbut-2-enyl-diphosphate synthase (1.17.7.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/011484-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (2.7.1.148)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07062-C-Methyl-D-erythritol 4-phosphate cytidylyltransferase (2.7.7.60)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/03Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027Isoprene synthase (4.2.3.27)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y406/00Phosphorus-oxygen lyases (4.6)
    • C12Y406/01Phosphorus-oxygen lyases (4.6.1)
    • C12Y406/010122-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (4.6.1.12)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
CA2901588A 2013-03-07 2014-03-06 Compositions and methods for biological production of isoprene Abandoned CA2901588A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361774342P 2013-03-07 2013-03-07
US61/774,342 2013-03-07
US201461928333P 2014-01-16 2014-01-16
US61/928,333 2014-01-16
PCT/US2014/021258 WO2014138419A1 (en) 2013-03-07 2014-03-06 Compositions and methods for biological production of isoprene

Publications (1)

Publication Number Publication Date
CA2901588A1 true CA2901588A1 (en) 2014-09-12

Family

ID=51491962

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2901588A Abandoned CA2901588A1 (en) 2013-03-07 2014-03-06 Compositions and methods for biological production of isoprene

Country Status (10)

Country Link
US (1) US20160017374A1 (enExample)
EP (1) EP2964748A4 (enExample)
JP (1) JP2016508737A (enExample)
KR (1) KR20150125653A (enExample)
CN (1) CN105008514A (enExample)
AU (1) AU2014225631A1 (enExample)
BR (1) BR112015021003A2 (enExample)
CA (1) CA2901588A1 (enExample)
RU (1) RU2015131480A (enExample)
WO (1) WO2014138419A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9695447B2 (en) * 2012-12-27 2017-07-04 Sekisui Chemical Co., Ltd. Recombinant cell and method for producing isoprene
CA2914641A1 (en) 2013-06-18 2014-12-24 Calysta, Inc. Compositions and methods for biological production of lactate from c1 compounds using lactate dehydrogenase transformants
JP2017504329A (ja) 2014-01-16 2017-02-09 キャリスタ, インコーポレイテッド アミノ酸の増強された産生のための微生物および関連する方法
DK3158065T3 (en) 2014-06-18 2019-04-08 Calysta Inc NUCLEIC ACIDS AND VECTORS FOR USE WITH METHANOTROPHIC BACTERIES
JP2016059313A (ja) * 2014-09-17 2016-04-25 積水化学工業株式会社 組換え細胞、並びに、イソプレン又は環式イソプレノイドの生産方法
DE102015103608A1 (de) * 2015-03-11 2016-09-15 Basf Se Verfahren zur mikrobiellen de novo Synthese von Terpenen
EP3292208B1 (en) 2015-05-06 2020-08-19 Trelys, Inc. Compositions and methods for biological production of methionine
EP3294758A1 (en) 2015-05-13 2018-03-21 Calysta, Inc. Proline auxotrophs
US20170145441A1 (en) * 2015-08-17 2017-05-25 INVISTA North America S.à.r.l. Methods, hosts, and reagents related thereto for production of unsaturated pentahydrocarbons, derivatives and intermediates thereof
US10533192B2 (en) 2015-12-22 2020-01-14 Nutech Ventures Production of isoprene by methane-producing archaea
KR102546640B1 (ko) * 2016-01-26 2023-06-22 에스케이이노베이션 주식회사 재조합 호염성 메탄균주를 이용한 이소프렌의 제조방법
JP2018014960A (ja) * 2016-07-29 2018-02-01 横浜ゴム株式会社 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド
CN109689857A (zh) * 2016-10-28 2019-04-26 积水化学工业株式会社 重组细胞、以及异戊二烯或萜的生产方法
WO2019006257A1 (en) * 2017-06-30 2019-01-03 Invista North America .S.A.R.L. METHODS, SYNTHETIC HOSTS AND REAGENTS FOR HYDROCARBON BIOSYNTHESIS
US11634733B2 (en) 2017-06-30 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof
US11505809B2 (en) 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis
US11180707B2 (en) * 2018-08-03 2021-11-23 Faramaz Fred Faramarzi System and method for duplicating flammable gas
CN109943493B (zh) * 2019-04-17 2021-05-18 天津大学 实现通用酶催化功能多样性的突变体菌株及其构建方法
JP7754406B2 (ja) * 2021-03-26 2025-10-15 三菱ケミカル株式会社 イソプレン産生能を有する遺伝子改変微生物およびこれを用いたイソプレン製造方法
CN119736218A (zh) * 2023-09-19 2025-04-01 西安卓虹超源生物科技有限公司 一种萜类化合物的生产及菌株
KR20250113943A (ko) * 2024-01-18 2025-07-28 한국생명공학연구원 메탄으로부터 이소프렌 생산능을 갖는 형질전환체

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818424B2 (en) * 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
ATE384119T1 (de) * 2000-09-29 2008-02-15 Cargill Inc Isoprenoidproduktion
US7947478B2 (en) * 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
BRPI0823506A8 (pt) * 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
CA2737223A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
TW201412988A (zh) * 2009-06-17 2014-04-01 Danisco Us Inc 使用dxp及mva途徑之改良之異戊二烯製造
DE102012012913A1 (de) * 2012-06-27 2014-01-02 Beatrice Saier Vorrichtung und Verfahren zur schaltbaren Förderung wenigstens eines Mediums
BR112015014843A2 (pt) * 2012-12-21 2017-10-10 Danisco Us Inc produção de isopreno, isoprenoide, e precursores de isoprenoide usando uma via de mevalonato inferior alternativa
US9695447B2 (en) * 2012-12-27 2017-07-04 Sekisui Chemical Co., Ltd. Recombinant cell and method for producing isoprene

Also Published As

Publication number Publication date
AU2014225631A1 (en) 2015-08-13
WO2014138419A1 (en) 2014-09-12
CN105008514A (zh) 2015-10-28
BR112015021003A2 (pt) 2017-07-18
JP2016508737A (ja) 2016-03-24
KR20150125653A (ko) 2015-11-09
EP2964748A4 (en) 2016-11-02
EP2964748A1 (en) 2016-01-13
US20160017374A1 (en) 2016-01-21
RU2015131480A (ru) 2017-04-12

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