CA2886247C - Microfluidic system for reproducing functional units of tissues and organs in vitro - Google Patents

Microfluidic system for reproducing functional units of tissues and organs in vitro Download PDF

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Publication number
CA2886247C
CA2886247C CA2886247A CA2886247A CA2886247C CA 2886247 C CA2886247 C CA 2886247C CA 2886247 A CA2886247 A CA 2886247A CA 2886247 A CA2886247 A CA 2886247A CA 2886247 C CA2886247 C CA 2886247C
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cells
matrix
cell
void
path
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English (en)
French (fr)
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CA2886247A1 (en
Inventor
Thomas Neumann
Anna A. TOUROVSKAIA
Mark E. Fauver
Greg Kramer
Elizabeth ASP
Henning MANN
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NORTIS Inc
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NORTIS Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/142—Apparatus
    • A01N1/143—Apparatus for organ perfusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A61P33/06—Antimalarials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5085—Supracellular entities, e.g. tissue, organisms of invertebrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
CA2886247A 2012-09-29 2013-09-27 Microfluidic system for reproducing functional units of tissues and organs in vitro Active CA2886247C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261707907P 2012-09-29 2012-09-29
US61/707,907 2012-09-29
US201261721002P 2012-10-31 2012-10-31
US61/721,002 2012-10-31
PCT/US2013/062307 WO2014052835A1 (en) 2012-09-29 2013-09-27 Microfluidic system for reproducing functional units of tissues and organs in vitro

Publications (2)

Publication Number Publication Date
CA2886247A1 CA2886247A1 (en) 2014-04-03
CA2886247C true CA2886247C (en) 2023-01-03

Family

ID=50389014

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2886247A Active CA2886247C (en) 2012-09-29 2013-09-27 Microfluidic system for reproducing functional units of tissues and organs in vitro

Country Status (9)

Country Link
US (3) US20150240194A1 (https=)
EP (1) EP2900801A4 (https=)
JP (1) JP6396909B2 (https=)
CN (1) CN104837982B (https=)
AU (1) AU2013323222A1 (https=)
CA (1) CA2886247C (https=)
HK (1) HK1213287A1 (https=)
IN (1) IN2015DN02696A (https=)
WO (1) WO2014052835A1 (https=)

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EP3161052A4 (en) * 2014-06-26 2018-03-21 Northeastern University Microfluidic device and method for analysis of tumor cell microenvironments
EP3584312A1 (en) * 2014-06-27 2019-12-25 Angiocrine Bioscience, Inc. Neural cells expressing adenovirus e4orf1, and methods of making and using the same
WO2016064757A1 (en) * 2014-10-19 2016-04-28 Nortis, Inc. Modular microfluidic system for perfused cell culture
WO2016081751A1 (en) * 2014-11-19 2016-05-26 Nortis, Inc. Method for vascularizing in-vitro generated or ex-vivo tissue fragments in a microfluidic device
GB2588276B (en) * 2015-04-24 2021-07-14 Harvard College Devices for simulating a function of a tissue and methods of use and manufacturing thereof
CA2983821A1 (en) * 2015-04-24 2017-01-05 President And Fellows Of Harvard College Devices for simulating a function of a tissue and methods of use and manufacturing thereof
CN104849439B (zh) * 2015-04-30 2017-01-18 四川农业大学 一种高效检测纳米颗粒肾毒性的方法
EP3334821A4 (en) * 2015-08-14 2019-07-17 The General Hospital Corporation SYSTEMS AND METHOD FOR USING BIOMIMETIC STRUCTURES FOR COMMUNICATION IN LIVING TISSUE
GB201613187D0 (en) * 2016-07-29 2016-09-14 Univ Central Lancashire Blood brain barrier model
WO2018026784A1 (en) * 2016-08-01 2018-02-08 University Of Georgia Research Foundation, Inc. In situ cell delivery using reconstituted photopolymerized chondroitin sulfate glycosaminoglycan hydrogel matrices
CN110997900B (zh) * 2017-07-14 2024-06-14 多伦多大学管理委员会 用于快速产生用于化合物筛选的类器官/球状体的微流体平台
US12036550B2 (en) 2017-08-28 2024-07-16 The Regents Of The University Of California Microfluidic device for the digestion of tissues into cellular suspensions
US11499128B2 (en) 2017-09-22 2022-11-15 The Regents Of The University Of Colorado Organ-on-chip microphysiological system
WO2019079725A1 (en) 2017-10-19 2019-04-25 Georgia Tech Research Corporation MESOFLUIDIC DEVICE FOR CULTIVATION OF CELLULAR AGGREGATES
KR102124420B1 (ko) * 2017-11-02 2020-06-18 한국과학기술연구원 선 스트레인의 조합 및 물성에 따른 구조체 내 미세섬유 정렬 제어 방법
EP3728552B1 (en) * 2017-12-22 2021-05-26 TissUse GmbH Microfluidic devices implementing blood and urine circuit for emulating homeostatic microphysiological system
CN111670245B (zh) * 2017-12-22 2024-03-12 蒂斯由斯股份有限公司 建立将iPSC衍生的细胞分化为器官等效物的新型多器官芯片
US11679546B2 (en) 2018-02-09 2023-06-20 The Regents Of The University Of Colorado, A Body Corporate Bioprinter and methods of manufacturing an organomimetic device
CN112154201B (zh) * 2018-05-22 2024-12-27 形态细胞科技公司 灌注生物反应器、灌注器件、人造肝脏系统及相关方法
US11480560B2 (en) 2018-06-11 2022-10-25 The Regents Of The University Of Colorado, A Body Corporate Delivery of aerosolized respiratory pathogens
GB201901273D0 (en) * 2019-01-30 2019-03-20 Stemnovate Ltd Method and apparatus for real time monitoring of cell and tissue culture
CN110108622B (zh) * 2019-06-17 2024-02-02 辽宁中医药大学 一种离体微血管内皮屏障功能检测方法与设备
CN110295111A (zh) * 2019-07-15 2019-10-01 清华大学深圳研究生院 一种仿生血管网络肿瘤细胞外渗迁移的装置
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
WO2021260735A1 (en) * 2020-06-26 2021-12-30 Ykrita Lifesciences Private Limited Bioengineered artificial lateral liver (ball) or bioengineered artificial ectopic liver (bael)
US20220062895A1 (en) * 2020-08-27 2022-03-03 Wisconsin Alumni Research Foundation Microfluidic Device And Method Of Assaying For Immune Cell Exhaustion Using Same
CA3211524A1 (en) * 2021-02-19 2022-08-25 The Regents Of The University Of California Microfluidic device for the digestion of tissues into cellular suspensions
CN112958172B (zh) * 2021-02-24 2021-12-28 北京航空航天大学 一种模拟体内微生物-肠-脑轴信号传导过程的仿生微流控芯片
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Also Published As

Publication number Publication date
EP2900801A4 (en) 2016-06-22
WO2014052835A1 (en) 2014-04-03
HK1213287A1 (zh) 2016-06-30
US20250066703A1 (en) 2025-02-27
EP2900801A1 (en) 2015-08-05
JP2015533083A (ja) 2015-11-19
CA2886247A1 (en) 2014-04-03
AU2013323222A1 (en) 2015-04-09
IN2015DN02696A (https=) 2015-09-04
US20150240194A1 (en) 2015-08-27
CN104837982B (zh) 2017-11-21
CN104837982A (zh) 2015-08-12
US20190062684A1 (en) 2019-02-28
JP6396909B2 (ja) 2018-09-26

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