CA2877493C - Procedes de pcr hautement multiplex et compositions - Google Patents

Procedes de pcr hautement multiplex et compositions Download PDF

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Publication number
CA2877493C
CA2877493C CA2877493A CA2877493A CA2877493C CA 2877493 C CA2877493 C CA 2877493C CA 2877493 A CA2877493 A CA 2877493A CA 2877493 A CA2877493 A CA 2877493A CA 2877493 C CA2877493 C CA 2877493C
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CA
Canada
Prior art keywords
dna
target
primers
primer
pcr
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CA2877493A
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English (en)
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CA2877493A1 (fr
Inventor
Bernhard Zimmermann
Matthew M. HILL
Philippe Gilbert LACROUTE
Michael Dodd
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Natera Inc
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Natera Inc
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Publication date
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Priority claimed from US13/683,604 external-priority patent/US20130123120A1/en
Publication of CA2877493A1 publication Critical patent/CA2877493A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/10—Ploidy or copy number detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

Abstract

L'invention concerne des procédés pour amplifier simultanément de multiples régions d'acide nucléique d'intérêt dans un volume de réaction ainsi que des procédés pour sélectionner une bibliothèque d'amorces utilisées dans des procédés d'amplification. L'invention concerne également une bibliothèque d'amorces avec des caractéristiques souhaitées, telle que la formation minimale de dimères d'amorce amplifiés ou d'autres amplicons non cibles.
CA2877493A 2012-07-24 2012-11-21 Procedes de pcr hautement multiplex et compositions Active CA2877493C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261675020P 2012-07-24 2012-07-24
US61/675,020 2012-07-24
PCT/US2012/066339 WO2014018080A1 (fr) 2012-07-24 2012-11-21 Procédés de pcr hautement multiplex et compositions
US13/683,604 US20130123120A1 (en) 2010-05-18 2012-11-21 Highly Multiplex PCR Methods and Compositions
US13/683,604 2012-11-21

Publications (2)

Publication Number Publication Date
CA2877493A1 CA2877493A1 (fr) 2014-01-30
CA2877493C true CA2877493C (fr) 2020-08-25

Family

ID=49997695

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2877493A Active CA2877493C (fr) 2012-07-24 2012-11-21 Procedes de pcr hautement multiplex et compositions

Country Status (10)

Country Link
JP (10) JP6392222B2 (fr)
KR (1) KR101890466B1 (fr)
CN (1) CN104685064A (fr)
AU (1) AU2012385961B9 (fr)
CA (1) CA2877493C (fr)
HK (1) HK1211058A1 (fr)
IL (1) IL236435A0 (fr)
RU (1) RU2650790C2 (fr)
SG (1) SG11201408813VA (fr)
WO (1) WO2014018080A1 (fr)

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Also Published As

Publication number Publication date
JP2018183189A (ja) 2018-11-22
JP2022037145A (ja) 2022-03-08
JP2020054402A (ja) 2020-04-09
JP2020058388A (ja) 2020-04-16
JP2015526073A (ja) 2015-09-10
JP6916153B2 (ja) 2021-08-11
JP2022051949A (ja) 2022-04-01
JP2022027971A (ja) 2022-02-14
CN104685064A (zh) 2015-06-03
KR101890466B1 (ko) 2018-08-21
JP6392222B2 (ja) 2018-09-19
AU2012385961B2 (en) 2017-04-13
WO2014018080A1 (fr) 2014-01-30
JP7343563B2 (ja) 2023-09-12
RU2650790C2 (ru) 2018-04-17
JP6997814B2 (ja) 2022-02-10
CA2877493A1 (fr) 2014-01-30
HK1211058A1 (en) 2016-05-13
JP6997815B2 (ja) 2022-02-10
JP6997813B2 (ja) 2022-02-10
JP7027468B2 (ja) 2022-03-01
IL236435A0 (en) 2015-02-26
RU2014152883A (ru) 2016-09-10
JP2020054400A (ja) 2020-04-09
JP2022027975A (ja) 2022-02-14
AU2012385961A1 (en) 2015-02-12
JP7348330B2 (ja) 2023-09-20
SG11201408813VA (en) 2015-02-27
JP2020054401A (ja) 2020-04-09
KR20150038216A (ko) 2015-04-08
AU2012385961B9 (en) 2017-05-18

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