CA2874832C - Recombinant microorganisms and uses therefor - Google Patents

Recombinant microorganisms and uses therefor Download PDF

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Publication number
CA2874832C
CA2874832C CA2874832A CA2874832A CA2874832C CA 2874832 C CA2874832 C CA 2874832C CA 2874832 A CA2874832 A CA 2874832A CA 2874832 A CA2874832 A CA 2874832A CA 2874832 C CA2874832 C CA 2874832C
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Canada
Prior art keywords
bacteria
clostridium
microorganism
nucleic acid
carboxydotrophic
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Active
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CA2874832A
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English (en)
French (fr)
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CA2874832A1 (en
Inventor
Wendy Chen
Michael Koepke
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Lanzatech NZ Inc
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Lanzatech New Zealand Ltd
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Publication of CA2874832A1 publication Critical patent/CA2874832A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2874832A 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor Active CA2874832C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261653348P 2012-05-30 2012-05-30
US61/653,348 2012-05-30
PCT/NZ2013/000092 WO2013180581A1 (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Publications (2)

Publication Number Publication Date
CA2874832A1 CA2874832A1 (en) 2013-12-05
CA2874832C true CA2874832C (en) 2016-02-02

Family

ID=49670706

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2874832A Active CA2874832C (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Country Status (8)

Country Link
US (1) US9994878B2 (index.php)
EP (1) EP2855690B1 (index.php)
JP (1) JP6445970B2 (index.php)
KR (1) KR102098849B1 (index.php)
CN (2) CN113832199A (index.php)
CA (1) CA2874832C (index.php)
IN (1) IN2014DN10168A (index.php)
WO (1) WO2013180581A1 (index.php)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
SG11201605775UA (en) 2014-01-28 2016-08-30 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
KR102531893B1 (ko) 2014-10-22 2023-05-11 란자테크 엔지, 인크. 멀티-스테이지 생물반응기 공정
US10113194B2 (en) 2014-10-22 2018-10-30 Lanzatech New Zealand Limited Gas testing unit and method
US10131884B2 (en) 2015-02-23 2018-11-20 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
CN104789516B (zh) * 2015-04-22 2017-12-22 天津大学 一种产三羟基丙酸的集胞藻6803基因工程菌及构建方法及应用
DE102016212497B4 (de) * 2015-07-13 2024-04-25 Sk Innovation Co., Ltd. Mutanter Mikroorganismus, der ein Gen umfasst, das Methylmalonyl-CoA-Reductase codiert, und seine Verwendung
MX2023003058A (es) 2015-10-13 2023-04-05 Lanzatech Nz Inc Bacteria diseñada por ingeniería genética que comprende una trayectoria de fermentación que genera energía.
EA201891335A1 (ru) 2015-12-03 2018-11-30 Ланцатек Нью Зилэнд Лимитед Добавление аргинина с целью повышения эффективности ацетогенов, ферментирующих газ
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
GB201601558D0 (en) 2016-01-28 2016-03-16 Verdant Bioproducts Ltd Method for producing 3-hydroxypropionamide
US10358662B2 (en) 2016-02-01 2019-07-23 Lanzatech New Zealand Limited Integrated fermentation and electrolysis process
AU2017222688B2 (en) 2016-02-26 2023-05-25 Lanzatech Nz, Inc. CRISPR/Cas systems for C-1 fixing bacteria
CA3024114C (en) 2016-05-14 2020-02-04 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
JPWO2018070141A1 (ja) * 2016-10-13 2019-08-08 積水化学工業株式会社 偏性嫌気性酢酸生成微生物、並びに、組換え微生物
PH12020550073A1 (en) 2017-09-08 2022-07-18 Lanzatech Inc Processes and systems for metabolite production using hydrogen rich c1- containing substrates
MY196897A (en) 2017-12-19 2023-05-09 Lanzatech Inc Microorganisms and methods for the biological production of ethylene glycol
CN111683731B (zh) 2018-02-12 2022-09-02 朗泽科技有限公司 用于提高碳转化效率的工艺
CN111918957B (zh) 2018-04-20 2024-11-08 朗泽科技有限公司 间歇电解流
CN109112156A (zh) * 2018-09-19 2019-01-01 江苏师范大学 一种生产3-羟基丙酸的重组假单胞菌、其构建方法及其应用
CN112955558A (zh) 2018-11-19 2021-06-11 朗泽科技有限公司 发酵和气化的集成
KR20240135053A (ko) 2019-02-08 2024-09-10 란자테크, 인크. 유사한 비등점 생성물의 회수 방법
EP3942058A1 (en) 2019-03-20 2022-01-26 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
MY197373A (en) 2019-07-11 2023-06-14 Lanzatech Inc Methods for optimizing gas utilization
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
JP2023518045A (ja) 2020-03-18 2023-04-27 ランザテク，インコーポレイテッド ガス基質からの２－フェニルエタノールの発酵産生
MX2022013570A (es) * 2020-04-29 2022-11-30 Lanzatech Inc Produccion fermentativa de b-cetoadipato a partir de sustratos gaseosos.
EP4162056A4 (en) 2020-06-06 2024-05-29 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
JP7596523B2 (ja) * 2021-02-08 2024-12-09 ランザテク，インコーポレイテッド 組換え微生物及びその使用
BR112023020761A2 (pt) 2021-04-09 2023-12-12 Lanzatech Inc Processo para fornecer uma matéria-prima, e, aparelho para fornecer uma matéria-prima de fermentação gasosa
WO2023004293A1 (en) 2021-07-20 2023-01-26 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
WO2023250382A1 (en) 2022-06-21 2023-12-28 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates
AU2023289395A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
WO2024036187A1 (en) 2022-08-10 2024-02-15 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
US12281344B2 (en) 2023-06-05 2025-04-22 Lanzatech, Inc. Integrated gas fermentation
WO2024253883A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation and carbon black processes
CN121065057A (zh) * 2025-11-05 2025-12-05 珠海麦得发生物科技股份有限公司 一种生产3-羟基丙酸的重组菌及其构建方法和应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
WO2002008438A2 (en) 2000-07-25 2002-01-31 Bioengineering Resources, Inc. Methods for increasing the production of ethanol from microbial fermentation
US7186541B2 (en) * 2000-11-20 2007-03-06 Cargill, Incorporated 3-hydroxypropionic acid and other organic compounds
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
CA2703622C (en) 2007-11-13 2014-12-16 Lanzatech New Zealand Limited Clostridium autoethanogenum strain and methods of use thereof to produce ethanol and acetate
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
CN101445813B (zh) * 2007-12-21 2013-03-27 清华大学 一种生产3-羟基丙酸的方法
WO2009089457A1 (en) * 2008-01-11 2009-07-16 Novozymes A/S Methods for producing 3-hydroxypropionic acid and compounds thereof
US7803589B2 (en) * 2008-01-22 2010-09-28 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
WO2009111513A1 (en) * 2008-03-03 2009-09-11 Joule Biotechnologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
CN101519635A (zh) * 2009-04-03 2009-09-02 南京工业大学 产3-羟基丙酸的转化微生物及其构建方法和应用
EP2933338A3 (en) * 2009-09-09 2016-01-06 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
WO2011063363A2 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an organic acid and/or related chemicals
JP2013513384A (ja) * 2009-12-10 2013-04-22 ジェノマティカ・インコーポレイテッド 合成ガスまたは他のガス状の炭素源およびメタノールを１，３−ブタンジオールへ変換するための方法および生物
US20110177564A1 (en) * 2010-01-15 2011-07-21 Massachusetts Institute Of Technology Bioprocess and microbe engineering for total carbon utilization in biofuel production
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
JP2012085635A (ja) 2010-03-23 2012-05-10 Nippon Shokubai Co Ltd ３−ヒドロキシプロピオン酸の製造方法
WO2012019175A2 (en) * 2010-08-06 2012-02-09 Mascoma Corporation Production of malonyl-coa drived products via anaerobic pathways
WO2012074818A2 (en) * 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
AU2012221176B2 (en) 2011-02-25 2015-03-19 Lanzatech Nz, Inc. Recombinant microorganisms and uses therefor
WO2013043758A2 (en) * 2011-09-19 2013-03-28 Opx Biotechnologies, Inc. Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products
US8349587B2 (en) * 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds
US20130288317A1 (en) * 2012-04-30 2013-10-31 Metabolix, Inc. Chemical Increased yields of phas from hydrogen feeding and diverse carbon fixation pathways

Also Published As

Publication number Publication date
JP6445970B2 (ja) 2018-12-26
US20130323806A1 (en) 2013-12-05
US9994878B2 (en) 2018-06-12
IN2014DN10168A (index.php) 2015-08-21
KR102098849B1 (ko) 2020-04-09
WO2013180581A1 (en) 2013-12-05
EP2855690B1 (en) 2018-07-11
CN104508136A (zh) 2015-04-08
CN113832199A (zh) 2021-12-24
EP2855690A1 (en) 2015-04-08
CA2874832A1 (en) 2013-12-05
KR20150021081A (ko) 2015-02-27
JP2015517823A (ja) 2015-06-25
EP2855690A4 (en) 2016-02-17

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