CA2874832C - Recombinant microorganisms and uses therefor - Google Patents

Recombinant microorganisms and uses therefor Download PDF

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Publication number
CA2874832C
CA2874832C CA2874832A CA2874832A CA2874832C CA 2874832 C CA2874832 C CA 2874832C CA 2874832 A CA2874832 A CA 2874832A CA 2874832 A CA2874832 A CA 2874832A CA 2874832 C CA2874832 C CA 2874832C
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Canada
Prior art keywords
bacteria
clostridium
microorganism
nucleic acid
carboxydotrophic
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Active
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CA2874832A
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English (en)
French (fr)
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CA2874832A1 (en
Inventor
Wendy Chen
Michael Koepke
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Lanzatech NZ Inc
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Lanzatech New Zealand Ltd
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Publication of CA2874832A1 publication Critical patent/CA2874832A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2874832A 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor Active CA2874832C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261653348P 2012-05-30 2012-05-30
US61/653,348 2012-05-30
PCT/NZ2013/000092 WO2013180581A1 (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Publications (2)

Publication Number Publication Date
CA2874832A1 CA2874832A1 (en) 2013-12-05
CA2874832C true CA2874832C (en) 2016-02-02

Family

ID=49670706

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2874832A Active CA2874832C (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Country Status (8)

Country Link
US (1) US9994878B2 (forum.php)
EP (1) EP2855690B1 (forum.php)
JP (1) JP6445970B2 (forum.php)
KR (1) KR102098849B1 (forum.php)
CN (2) CN113832199A (forum.php)
CA (1) CA2874832C (forum.php)
IN (1) IN2014DN10168A (forum.php)
WO (1) WO2013180581A1 (forum.php)

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US10131884B2 (en) 2015-02-23 2018-11-20 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
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EA201890952A1 (ru) 2015-10-13 2018-09-28 Ланцатек Нью Зилэнд Лимитед Генетически сконструированная бактерия, содержащая энергогенерирующий ферментативный путь
CA3010412C (en) 2015-12-03 2020-04-14 Lanzatech New Zealand Limited Arginine supplementation to improve efficiency in gas fermenting acetogens
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
GB201601558D0 (en) 2016-01-28 2016-03-16 Verdant Bioproducts Ltd Method for producing 3-hydroxypropionamide
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US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
WO2021188190A1 (en) 2020-03-18 2021-09-23 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
CN114144513A (zh) * 2020-04-29 2022-03-04 朗泽科技有限公司 从气态底物发酵生产β-酮己二酸盐
US11760989B2 (en) 2020-06-06 2023-09-19 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
CA3195088C (en) 2021-02-08 2024-04-23 Fungmin Liew Recombinant microorganisms and their use in the production of 3-hydroxypropionate [3-hp]
JP2024513395A (ja) 2021-04-09 2024-03-25 ランザテク，インコーポレイテッド 供給原料を提供するためのプロセス及び装置
US12241105B2 (en) 2021-07-20 2025-03-04 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
CN119452090A (zh) 2022-06-21 2025-02-14 朗泽科技有限公司 用于由c1底物连续共产生串联重复蛋白和化学产物的微生物和方法
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Also Published As

Publication number Publication date
CN113832199A (zh) 2021-12-24
US9994878B2 (en) 2018-06-12
KR20150021081A (ko) 2015-02-27
US20130323806A1 (en) 2013-12-05
JP6445970B2 (ja) 2018-12-26
WO2013180581A1 (en) 2013-12-05
CN104508136A (zh) 2015-04-08
CA2874832A1 (en) 2013-12-05
EP2855690A1 (en) 2015-04-08
EP2855690B1 (en) 2018-07-11
IN2014DN10168A (forum.php) 2015-08-21
EP2855690A4 (en) 2016-02-17
JP2015517823A (ja) 2015-06-25
KR102098849B1 (ko) 2020-04-09

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