CA2867789C - Methods for detection of anti-cytomegalovirus neutralizing antibodies - Google Patents
Methods for detection of anti-cytomegalovirus neutralizing antibodies Download PDFInfo
- Publication number
- CA2867789C CA2867789C CA2867789A CA2867789A CA2867789C CA 2867789 C CA2867789 C CA 2867789C CA 2867789 A CA2867789 A CA 2867789A CA 2867789 A CA2867789 A CA 2867789A CA 2867789 C CA2867789 C CA 2867789C
- Authority
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- Canada
- Prior art keywords
- hcmv
- infection
- fluorescence
- cells
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55505—Inorganic adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
- G01N2333/04—Varicella-zoster virus
- G01N2333/045—Cytomegalovirus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Physiology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261616204P | 2012-03-27 | 2012-03-27 | |
| US61/616,204 | 2012-03-27 | ||
| PCT/IB2013/001021 WO2013144722A2 (en) | 2012-03-27 | 2013-03-27 | Methods for detection of anti-cytomegalovirus neutralizing antibodies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2867789A1 CA2867789A1 (en) | 2013-10-03 |
| CA2867789C true CA2867789C (en) | 2022-06-14 |
Family
ID=49261346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2867789A Active CA2867789C (en) | 2012-03-27 | 2013-03-27 | Methods for detection of anti-cytomegalovirus neutralizing antibodies |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US10006096B2 (https=) |
| EP (2) | EP3613431B1 (https=) |
| JP (1) | JP6265344B2 (https=) |
| KR (2) | KR102146340B1 (https=) |
| CN (2) | CN104321444A (https=) |
| AU (2) | AU2013239383B2 (https=) |
| BR (1) | BR112014023913B1 (https=) |
| CA (1) | CA2867789C (https=) |
| MX (1) | MX354822B (https=) |
| RU (1) | RU2660712C2 (https=) |
| WO (1) | WO2013144722A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3613431B1 (en) * | 2012-03-27 | 2023-01-11 | Variation Biotechnologies Inc. | Methods for detection of anti-cytomegalovirus neutralizing antibodies |
| US10611800B2 (en) | 2016-03-11 | 2020-04-07 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| JP6850483B2 (ja) * | 2018-03-06 | 2021-03-31 | 株式会社ＣｅＳＰＩＡ | Ｃｅｌｌ−ｂａｓｅｄ ａｓｓａｙ法に基づいたハイスループット抗体検出方法 |
| WO2019232453A1 (en) * | 2018-06-01 | 2019-12-05 | Gravid Therapies, Inc. | Methods, kits, and products for detection of cytomegalovirus infection |
| AU2019322828A1 (en) * | 2018-08-14 | 2021-02-25 | Merck Sharp & Dohme Llc | A virus neutralization assay utilizing imaging cytometry |
| US11629172B2 (en) | 2018-12-21 | 2023-04-18 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| CN111474339A (zh) * | 2020-04-17 | 2020-07-31 | 浙江康佰裕生物科技有限公司 | 一种利用荧光标记痘病毒颗粒的方法及其应用 |
| TWI810589B (zh) | 2020-06-21 | 2023-08-01 | 美商輝瑞股份有限公司 | 人巨細胞病毒糖蛋白Ｂ（ｇＢ）多肽 |
| CN112362649B (zh) * | 2020-11-09 | 2024-07-12 | 长春金赛药业有限责任公司 | 一种rhGH中和抗体的检测方法 |
| CN112945919B (zh) * | 2021-01-29 | 2023-04-28 | 上海睿钰生物科技有限公司 | 一种病毒中和抗体的检测方法、系统及其应用 |
| CN114859046B (zh) * | 2021-04-30 | 2025-11-28 | 中国人民解放军军事科学院军事医学研究院 | 含高效价抗hcmv中和抗体原料血浆的筛选方法和抗hcmv中和抗体效价的检测方法 |
| WO2023141485A2 (en) * | 2022-01-19 | 2023-07-27 | Trustees Of Dartmouth College | Systems and methods for deterring viral transmission using neural networks |
| US20250140407A1 (en) * | 2022-02-11 | 2025-05-01 | Trustees Of Dartmouth College | Systems and methods for identifying and treating primary and latent infections and/or determining time since infection |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6267965B1 (en) | 1981-12-24 | 2001-07-31 | Virogenetics Corporation | Recombinant poxvirus—cytomegalovirus compositions and uses |
| US5248768A (en) * | 1986-11-24 | 1993-09-28 | The Children's Hospital, Incorporated | Immunogenic glycoproteins of human cytomegalovirus |
| JPH06222062A (ja) | 1993-01-22 | 1994-08-12 | Teijin Ltd | ウイルス感染価および中和抗体価の測定法 |
| DE19910044A1 (de) | 1999-03-08 | 2000-09-14 | Bodo Plachter | Virale Partikel, die nach Infektion durch humanes Cytomegalovirus freigesetzt werden und ihre Verwendung als Impfstoff |
| US6569616B1 (en) * | 1999-07-29 | 2003-05-27 | Wisconsin Alumni Research Foundation | Human cytomegalovirus glycoprotein O as a new drug target and subunit vaccine candidate |
| US6849409B2 (en) * | 2000-10-16 | 2005-02-01 | Axxima Pharmaceuticals Ag | Cellular kinases involved in Cytomegalovirus infection and their inhibition |
| DK2311848T3 (da) | 2002-12-23 | 2013-10-14 | Vical Inc | Kodon-optimerede polynukleotidbaserede vacciner mod human cytomegalovirus-infektion |
| DE602004021260D1 (de) | 2003-07-11 | 2009-07-09 | Alphavax Inc | Cytomegalovirusimpfstoffe, die auf dem alphavirus basieren |
| CN1686541A (zh) * | 2005-04-11 | 2005-10-26 | 王明丽 | 人巨细胞病毒pp65蛋白质疫苗的制备方法 |
| CA2662164A1 (en) * | 2006-09-01 | 2008-03-06 | Stirling John Edwards | Method of eliciting or inducing an immune response |
| US9439960B2 (en) * | 2007-10-10 | 2016-09-13 | The Trustees Of Princeton University | Cytomegalovirus vaccines and methods of production |
| AU2008359583B2 (en) * | 2008-07-16 | 2012-04-05 | Institute For Research In Biomedicine | Human cytomegalovirus neutralising antibodies and use thereof |
| US20100267583A1 (en) | 2009-04-15 | 2010-10-21 | Variation Biotechnologies Inc. | Method and kit for detection of hepatitis a virus neutralizing antibodies |
| US9145577B2 (en) | 2010-08-10 | 2015-09-29 | The Research Foundation Of State University Of New York | Lipoprotein lipase assay |
| CA2889659C (en) * | 2011-11-11 | 2023-03-14 | Variation Biotechnologies Inc. | Compositions and methods for treatment of cytomegalovirus |
| EP3613431B1 (en) * | 2012-03-27 | 2023-01-11 | Variation Biotechnologies Inc. | Methods for detection of anti-cytomegalovirus neutralizing antibodies |
-
2013
- 2013-03-27 EP EP19199107.4A patent/EP3613431B1/en active Active
- 2013-03-27 CN CN201380026015.9A patent/CN104321444A/zh active Pending
- 2013-03-27 EP EP13769863.5A patent/EP2831285B1/en active Active
- 2013-03-27 US US14/387,870 patent/US10006096B2/en active Active
- 2013-03-27 JP JP2015502478A patent/JP6265344B2/ja active Active
- 2013-03-27 CA CA2867789A patent/CA2867789C/en active Active
- 2013-03-27 WO PCT/IB2013/001021 patent/WO2013144722A2/en not_active Ceased
- 2013-03-27 RU RU2014142815A patent/RU2660712C2/ru active
- 2013-03-27 BR BR112014023913-4A patent/BR112014023913B1/pt not_active IP Right Cessation
- 2013-03-27 AU AU2013239383A patent/AU2013239383B2/en not_active Ceased
- 2013-03-27 KR KR1020147030138A patent/KR102146340B1/ko active Active
- 2013-03-27 KR KR1020207021568A patent/KR102314245B1/ko active Active
- 2013-03-27 CN CN202411673083.0A patent/CN119716047A/zh active Pending
- 2013-03-27 MX MX2014011504A patent/MX354822B/es active IP Right Grant
-
2018
- 2018-05-29 US US15/991,044 patent/US10161010B2/en active Active
- 2018-11-27 AU AU2018271276A patent/AU2018271276B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| KR20140146630A (ko) | 2014-12-26 |
| WO2013144722A2 (en) | 2013-10-03 |
| EP3613431A1 (en) | 2020-02-26 |
| AU2018271276B2 (en) | 2021-06-03 |
| MX2014011504A (es) | 2015-03-13 |
| RU2014142815A (ru) | 2016-05-20 |
| KR20200095575A (ko) | 2020-08-10 |
| US10006096B2 (en) | 2018-06-26 |
| CA2867789A1 (en) | 2013-10-03 |
| AU2013239383A1 (en) | 2014-10-16 |
| BR112014023913A2 (https=) | 2017-06-20 |
| BR112014023913B1 (pt) | 2022-08-09 |
| EP2831285A2 (en) | 2015-02-04 |
| EP3613431B1 (en) | 2023-01-11 |
| US10161010B2 (en) | 2018-12-25 |
| CN119716047A (zh) | 2025-03-28 |
| US20180274044A1 (en) | 2018-09-27 |
| AU2018271276A1 (en) | 2018-12-20 |
| RU2660712C2 (ru) | 2018-07-09 |
| CN104321444A (zh) | 2015-01-28 |
| EP2831285B1 (en) | 2019-10-30 |
| MX354822B (es) | 2018-03-21 |
| JP6265344B2 (ja) | 2018-01-24 |
| EP2831285A4 (en) | 2015-10-28 |
| JP2015518140A (ja) | 2015-06-25 |
| KR102146340B1 (ko) | 2020-08-24 |
| KR102314245B1 (ko) | 2021-10-20 |
| US20150044668A1 (en) | 2015-02-12 |
| WO2013144722A3 (en) | 2013-11-28 |
| AU2013239383B2 (en) | 2018-10-04 |
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Legal Events
| Date | Code | Title | Description |
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| EEER | Examination request |
Effective date: 20180117 |
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| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-4-4-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20251009 |
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| H13 | Ip right lapsed |
Free format text: ST27 STATUS EVENT CODE: N-4-6-H10-H13-H100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE AND LATE FEE NOT PAID BY DEADLINE OF NOTICE Effective date: 20260122 |
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| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260122 |