CA2848064A1 - Eukaryotic organisms and methods for producing 1,3-butanediol - Google Patents

Eukaryotic organisms and methods for producing 1,3-butanediol Download PDF

Info

Publication number
CA2848064A1
CA2848064A1 CA2848064A CA2848064A CA2848064A1 CA 2848064 A1 CA2848064 A1 CA 2848064A1 CA 2848064 A CA2848064 A CA 2848064A CA 2848064 A CA2848064 A CA 2848064A CA 2848064 A1 CA2848064 A1 CA 2848064A1
Authority
CA
Canada
Prior art keywords
coa
acetyl
pathway comprises
bdo
pathway
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2848064A
Other languages
English (en)
French (fr)
Inventor
Anthony P. Burgard
Mark J. Burk
Robin E. Osterhout
Priti Pharkya
Jingyi Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of CA2848064A1 publication Critical patent/CA2848064A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
    • C08G63/02—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds
    • C08G63/12—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from polycarboxylic acids and polyhydroxy compounds
    • C08G63/16—Dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CA2848064A 2011-09-08 2012-09-07 Eukaryotic organisms and methods for producing 1,3-butanediol Abandoned CA2848064A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201161532492P 2011-09-08 2011-09-08
US61/532,492 2011-09-08
US201161541951P 2011-09-30 2011-09-30
US61/541,951 2011-09-30
US201161558959P 2011-11-11 2011-11-11
US61/558,959 2011-11-11
US201261649039P 2012-05-18 2012-05-18
US61/649,039 2012-05-18
US201261655355P 2012-06-04 2012-06-04
US61/655,355 2012-06-04
PCT/US2012/054152 WO2013036764A1 (en) 2011-09-08 2012-09-07 Eukaryotic organisms and methods for producing 1,3-butanediol

Publications (1)

Publication Number Publication Date
CA2848064A1 true CA2848064A1 (en) 2013-03-14

Family

ID=47830417

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2848064A Abandoned CA2848064A1 (en) 2011-09-08 2012-09-07 Eukaryotic organisms and methods for producing 1,3-butanediol

Country Status (10)

Country Link
US (2) US20130066035A1 (US06559137-20030506-C00071.png)
EP (1) EP2753699A4 (US06559137-20030506-C00071.png)
KR (1) KR20140058691A (US06559137-20030506-C00071.png)
CN (1) CN103917651A (US06559137-20030506-C00071.png)
BR (1) BR112014005352A2 (US06559137-20030506-C00071.png)
CA (1) CA2848064A1 (US06559137-20030506-C00071.png)
IN (1) IN2014CN02584A (US06559137-20030506-C00071.png)
MX (1) MX2014002787A (US06559137-20030506-C00071.png)
SG (1) SG11201400466TA (US06559137-20030506-C00071.png)
WO (1) WO2013036764A1 (US06559137-20030506-C00071.png)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
EP2899270A4 (en) * 2012-09-24 2016-07-20 Showa Denko Kk PROCESS FOR PREPARING BUTANEDIOL
CN105209414B (zh) 2013-03-15 2018-04-17 基因组股份公司 用于从发酵肉汤中获得1,4‑丁二醇的方法和系统
BR112015020904A2 (pt) 2013-03-15 2017-10-10 Genomatica Inc microrganismos e métodos para produção de butadieno e compostos relacionados por assimilação de formiato
EP3008178A1 (en) 2013-06-14 2016-04-20 Technical University of Denmark Microbial production of 3-hydroxypropionic acid
WO2015057154A2 (en) 2013-10-18 2015-04-23 Biopetrolia Ab ENGINEERING OF ACETYL-CoA METABOLISM IN YEAST
US20160376600A1 (en) * 2013-11-25 2016-12-29 Genomatica, Inc. Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol
US10808262B2 (en) 2013-12-03 2020-10-20 Genomatica, Inc. Microorganisms and methods for improving product yields on methanol using acetyl-CoA synthesis
ES2800606T3 (es) 2013-12-27 2021-01-04 Genomatica Inc Métodos y organismos con mayores eficiencias del flujo de carbono
US20170298363A1 (en) 2014-09-18 2017-10-19 Genomatica, Inc Non-natural microbial organisms with improved energetic efficiency
WO2016092063A1 (en) 2014-12-12 2016-06-16 Versalis S.P.A. Process for the production of 1,3 butadiene from 1,3 butanediol
CN107108398B (zh) 2015-02-23 2023-10-10 维尔萨利斯股份公司 用于含氧化合物的脱水的方法
BR112017025554B1 (pt) 2015-05-30 2024-03-12 Genomatica, Inc. Composição, e, método para produzir um polímero, resina ou artigo de fabricação
CN104988162B (zh) * 2015-06-08 2020-03-17 天津大学 辅因子再生模块在增强次级代谢产物在微生物中合成的应用
CN104988168A (zh) * 2015-06-08 2015-10-21 天津大学 乙酰辅酶a增强模块在增强7-脱氢胆甾醇在微生物中合成的应用
KR101704212B1 (ko) 2015-06-12 2017-02-08 씨제이제일제당 (주) 젖산을 생산하는 미생물 및 이를 이용한 젖산 제조 방법
US20170009263A1 (en) * 2015-07-08 2017-01-12 Invista North America S.A.R.L. Methods and host cells for enhancing production of 1, 3-butanediol
CA2992794A1 (en) * 2015-07-21 2017-01-26 Radhakrishnan Mahadevan Methods and microorganisms for the production of 1,3-butanediol
DK3359655T3 (da) * 2015-10-06 2020-02-03 Dsm Ip Assets Bv Eukaryot celle med forøget produktion af fermenteringsprodukt
CN108779153B (zh) 2015-10-30 2022-09-06 基因组股份公司 甲醇脱氢酶融合蛋白
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11299716B2 (en) 2017-03-31 2022-04-12 Genomatica, Inc. Aldehyde dehydrogenase variants and methods of use
WO2018183640A1 (en) 2017-03-31 2018-10-04 Genomatica, Inc. 3-hydroxybutyryl-coa dehydrogenase variants and methods of use
EP3652321B9 (en) * 2017-07-11 2023-10-04 Adisseo France S.A.S. Enhanced metabolite-producing saccharomyces cerevisiae
WO2019014310A1 (en) * 2017-07-13 2019-01-17 Verdezyne (Abc), Llc BIOLOGICAL METHODS FOR THE PREPARATION OF TERPENES
WO2019014309A1 (en) * 2017-07-13 2019-01-17 Verdezyne (Abc), Llc BIOLOGICAL METHODS FOR MODIFYING A CELL CARBON FLOW
US20210079334A1 (en) 2018-01-30 2021-03-18 Genomatica, Inc. Fermentation systems and methods with substantially uniform volumetric uptake rate of a reactive gaseous component
EP3814515A2 (en) 2018-06-26 2021-05-05 Genomatica, Inc. Engineered microorganisms with g3p---> 3pg enzyme and/or fructose-1,6-bisphosphatase including those having synthetic or enhanced methylotrophy
IL281601B2 (en) 2018-09-21 2024-03-01 Versalis Spa A method for purifying bio-3,1-butanediol from fermentation liquid
KR20210068489A (ko) 2018-09-26 2021-06-09 게노마티카 인코포레이티드 알데하이드 데하이드로게나제 변이체 및 이의 이용 방법
WO2020077059A1 (en) * 2018-10-12 2020-04-16 Yield10 Bioscience, Inc. Genetically engineered plants that express a quinone-utilizing malate dehydrogenase
JP6668577B1 (ja) 2018-10-30 2020-03-18 ＧｒｅｅｎＥａｒｔｈＩｎｓｔｉｔｕｔｅ株式会社 １，３−プロパンジオールの製造方法
CN110564662B (zh) * 2019-09-30 2022-03-25 南京农业大学 一种整合型高效表达乙醛脱氢酶枯草杆菌的构建方法
CN117701489B (zh) * 2024-02-05 2024-05-10 北京绿色康成生物技术有限公司 一种提高大肠杆菌生产1,3-丁二醇的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0827541B1 (en) * 1995-05-18 2009-11-18 University of Stellenbosch Nucleic acid sequence coding for a malate permease from s. pombe and uses thereof
US8956850B2 (en) * 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
US8691555B2 (en) * 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
CA2775390C (en) * 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
SG181607A1 (en) * 2009-12-10 2012-07-30 Genomatica Inc Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol
US8871488B2 (en) * 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases

Also Published As

Publication number Publication date
US20170247709A1 (en) 2017-08-31
KR20140058691A (ko) 2014-05-14
EP2753699A4 (en) 2015-08-19
US20130066035A1 (en) 2013-03-14
CN103917651A (zh) 2014-07-09
WO2013036764A1 (en) 2013-03-14
EP2753699A1 (en) 2014-07-16
IN2014CN02584A (US06559137-20030506-C00071.png) 2015-08-07
BR112014005352A2 (pt) 2017-03-28
MX2014002787A (es) 2014-12-08
AU2012304428B2 (en) 2016-03-17
SG11201400466TA (en) 2014-04-28
AU2012304428A1 (en) 2013-05-02

Similar Documents

Publication Publication Date Title
CA2848064A1 (en) Eukaryotic organisms and methods for producing 1,3-butanediol
US20210147882A1 (en) Electron consuming ethanol production pathway to displace glycerol formation in s. cerevisiae
Lian et al. Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R, 3R)-butanediol
Rodrigues et al. Sugar metabolism in yeasts: an overview of aerobic and anaerobic glucose catabolism
AU2016277014B2 (en) Microorganism producing lactic acid and method for producing lactic acid using same
US10190139B2 (en) Method for lactic acid production using Monascus strains modified for lactic acid production
JP2013515507A (ja) 組換え宿主細胞におけるヘキソースキナーゼの発現
US11339398B2 (en) Acid-resistant yeast with suppressed ethanol production pathway and method for producing lactic acid using same
US20140322779A1 (en) Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
Vivek et al. Recent advances in microbial biosynthesis of C3–C5 diols: genetics and process engineering approaches
AU2015293864A1 (en) Method for producing acetoin
WO2010051324A1 (en) Microaerobic cultures for converting glycerol to chemicals
Kang et al. L‐malic acid production from xylose by engineered Saccharomyces cerevisiae
Lee et al. Rewiring yeast metabolism for producing 2, 3-butanediol and two downstream applications: Techno-economic analysis and life cycle assessment of methyl ethyl ketone (MEK) and agricultural biostimulant production
EP3788135A1 (en) Materials and methods for controlling oxidation and reduction in biosynthetic pathways of species of the genera ralstonia and cupriavidus and organisms related thereto
Wang et al. Developing clostridia as cell factories for short-and medium-chain ester production
Tang et al. Recent developments in the microbial production of 1, 3-propanediol
ES2776361T3 (es) Cepas de microorganismo para la producción de 2,3-butanodiol
US11999943B2 (en) Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
KR20200045580A (ko) 재조합 배수체 효모를 이용한 2,3-부탄다이올의 생산방법
SI24955A (sl) Modificirana 6-fosfofrukto-1-kinaza, ki rekombinantnim celicam kvasovke Saccharomyces cerevisiae omogoča fermentativno uporabo pentoznih sladkorjev
WO2023168233A1 (en) Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
JP2021153524A (ja) 有機酸の製造方法
Lian Engineering Saccharomyces cerevisiae with enhanced supply of precursor metabolites for efficient production of fuels and chemicals
AU2007203093A2 (en) Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida Tropicalis, method of producing high-yield of xylitol using the same, and xylitol produced thereby

Legal Events

Date Code Title Description
FZDE Dead

Effective date: 20170907