CA2837634C - Polycistronic expression system for bacteria - Google Patents

Polycistronic expression system for bacteria Download PDF

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Publication number
CA2837634C
CA2837634C CA2837634A CA2837634A CA2837634C CA 2837634 C CA2837634 C CA 2837634C CA 2837634 A CA2837634 A CA 2837634A CA 2837634 A CA2837634 A CA 2837634A CA 2837634 C CA2837634 C CA 2837634C
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Prior art keywords
gram
gene
positive bacterium
nucleic acid
promoter
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CA2837634A
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English (en)
French (fr)
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CA2837634A1 (en
Inventor
Klaas Vandenbroucke
Karolien Van Huynegem
Lothar Steidler
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Intrexon Actobiotics NV
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Intrexon Actobiotics NV
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
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    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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    • A61P37/02—Immunomodulators
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    • A61P37/08—Antiallergic agents
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    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

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  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Neurology (AREA)
  • Diabetes (AREA)
  • Neurosurgery (AREA)
  • Hematology (AREA)
  • Pulmonology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Pain & Pain Management (AREA)
  • Oncology (AREA)
  • Obesity (AREA)
  • Dermatology (AREA)
CA2837634A 2011-06-01 2012-06-01 Polycistronic expression system for bacteria Active CA2837634C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3136084A CA3136084A1 (en) 2011-06-01 2012-06-01 Polycistronic expression system for bacteria

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11168495.7 2011-06-01
EP11168495 2011-06-01
EP11173588 2011-07-12
EP11173588.2 2011-07-12
PCT/EP2012/060431 WO2012164083A1 (en) 2011-06-01 2012-06-01 Polycistronic expression system for bacteria

Related Child Applications (1)

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CA3136084A Division CA3136084A1 (en) 2011-06-01 2012-06-01 Polycistronic expression system for bacteria

Publications (2)

Publication Number Publication Date
CA2837634A1 CA2837634A1 (en) 2012-12-06
CA2837634C true CA2837634C (en) 2021-12-14

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CA2837634A Active CA2837634C (en) 2011-06-01 2012-06-01 Polycistronic expression system for bacteria
CA3136084A Abandoned CA3136084A1 (en) 2011-06-01 2012-06-01 Polycistronic expression system for bacteria

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US (3) US9920324B2 (cg-RX-API-DMAC10.html)
EP (2) EP2714912B1 (cg-RX-API-DMAC10.html)
JP (3) JP6175428B2 (cg-RX-API-DMAC10.html)
KR (1) KR102066292B1 (cg-RX-API-DMAC10.html)
CN (2) CN103562390B (cg-RX-API-DMAC10.html)
AU (2) AU2012264589B2 (cg-RX-API-DMAC10.html)
BR (1) BR112013030824A8 (cg-RX-API-DMAC10.html)
CA (2) CA2837634C (cg-RX-API-DMAC10.html)
DK (1) DK2714912T3 (cg-RX-API-DMAC10.html)
ES (1) ES2676877T3 (cg-RX-API-DMAC10.html)
HU (1) HUE039577T2 (cg-RX-API-DMAC10.html)
RU (2) RU2644346C2 (cg-RX-API-DMAC10.html)
WO (1) WO2012164083A1 (cg-RX-API-DMAC10.html)

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US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP7292877B2 (ja) 2016-01-14 2023-06-19 イントレクソン・アクトバイオテイクス・エヌブイ １型糖尿病を処置するための組成物および方法
US10858663B2 (en) 2016-09-02 2020-12-08 Intrexon Actobiotics N.V. Genetically modified bacteria stably expressing IL-10 and insulin
KR102469701B1 (ko) * 2016-09-13 2022-11-22 인트랙슨 액토바이오틱스 엔.브이. 점막부착성 미생물
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
GB201906775D0 (en) * 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
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US10988770B2 (en) 2021-04-27
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