CA2810931C - Direct capture, amplification and sequencing of target dna using immobilized primers - Google Patents

Direct capture, amplification and sequencing of target dna using immobilized primers Download PDF

Info

Publication number
CA2810931C
CA2810931C CA2810931A CA2810931A CA2810931C CA 2810931 C CA2810931 C CA 2810931C CA 2810931 A CA2810931 A CA 2810931A CA 2810931 A CA2810931 A CA 2810931A CA 2810931 C CA2810931 C CA 2810931C
Authority
CA
Canada
Prior art keywords
sequence
population
adaptor
primer
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2810931A
Other languages
English (en)
French (fr)
Other versions
CA2810931A1 (en
Inventor
Samuel Myllykangas
Jason Buenrostro
Hanlee P. Ji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of CA2810931A1 publication Critical patent/CA2810931A1/en
Application granted granted Critical
Publication of CA2810931C publication Critical patent/CA2810931C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2810931A 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers Active CA2810931C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38639010P 2010-09-24 2010-09-24
US61/386,390 2010-09-24
US201161485062P 2011-05-11 2011-05-11
US61/485,062 2011-05-11
PCT/US2011/052645 WO2012040387A1 (en) 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers

Publications (2)

Publication Number Publication Date
CA2810931A1 CA2810931A1 (en) 2012-03-29
CA2810931C true CA2810931C (en) 2018-04-17

Family

ID=45874160

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2810931A Active CA2810931C (en) 2010-09-24 2011-09-21 Direct capture, amplification and sequencing of target dna using immobilized primers

Country Status (13)

Country Link
US (3) US9309556B2 (forum.php)
EP (2) EP2619329B1 (forum.php)
JP (1) JP5986572B2 (forum.php)
KR (1) KR20130113447A (forum.php)
CN (1) CN103228798B (forum.php)
AU (1) AU2011305445B2 (forum.php)
CA (1) CA2810931C (forum.php)
IL (1) IL225109A (forum.php)
IN (1) IN2013MN00522A (forum.php)
MX (1) MX346956B (forum.php)
NZ (1) NZ608313A (forum.php)
RU (1) RU2565550C2 (forum.php)
WO (1) WO2012040387A1 (forum.php)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
GB2467691A (en) 2008-09-05 2010-08-11 Aueon Inc Methods for stratifying and annotating cancer drug treatment options
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
CA2770389C (en) * 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CA2794522C (en) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
NZ608313A (en) 2010-09-24 2013-12-20 Univ Leland Stanford Junior Direct capture, amplification and sequencing of target dna using immobilized primers
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2768983A4 (en) 2011-10-17 2015-06-03 Good Start Genetics Inc METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES
CN104080958A (zh) 2011-10-19 2014-10-01 纽亘技术公司 用于定向核酸扩增和测序的组合物和方法
JP6193252B2 (ja) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド 高効率電子配列決定及び検出のためのシステム並びに方法
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3495503A1 (en) * 2012-03-05 2019-06-12 President and Fellows of Harvard College Systems and methods for epigenetic sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) * 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CN108018343B (zh) 2012-05-10 2023-01-03 通用医疗公司 用于测定核苷酸序列的方法
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN104812947B (zh) * 2012-07-17 2018-04-27 考希尔股份有限公司 检测遗传变异的系统和方法
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
CN103571822B (zh) * 2012-07-20 2016-03-30 中国科学院植物研究所 一种用于新一代测序分析的多重目的dna片段富集方法
ES2769241T5 (es) * 2012-09-04 2023-05-30 Guardant Health Inc Sistemas y métodos para detectar variación en el número de copias
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
SG10201704633UA (en) * 2012-12-10 2017-07-28 Resolution Bioscience Inc Methods for targeted genomic analysis
EP2959016A4 (en) * 2013-02-21 2016-10-12 Toma Biosciences Inc METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
US9898575B2 (en) 2013-08-21 2018-02-20 Seven Bridges Genomics Inc. Methods and systems for aligning sequences
US9116866B2 (en) 2013-08-21 2015-08-25 Seven Bridges Genomics Inc. Methods and systems for detecting sequence variants
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US11049587B2 (en) 2013-10-18 2021-06-29 Seven Bridges Genomics Inc. Methods and systems for aligning sequences in the presence of repeating elements
US10832797B2 (en) 2013-10-18 2020-11-10 Seven Bridges Genomics Inc. Method and system for quantifying sequence alignment
WO2015058093A1 (en) 2013-10-18 2015-04-23 Seven Bridges Genomics Inc. Methods and systems for genotyping genetic samples
SG11201603039PA (en) 2013-10-18 2016-05-30 Seven Bridges Genomics Inc Methods and systems for identifying disease-induced mutations
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US9063914B2 (en) 2013-10-21 2015-06-23 Seven Bridges Genomics Inc. Systems and methods for transcriptome analysis
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
US11473140B2 (en) * 2013-11-26 2022-10-18 Lc Sciences Lc Highly selective omega primer amplification of nucleic acid sequences
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
AU2014361815A1 (en) * 2013-12-15 2016-05-26 Academia Sinica Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
HK1225758A1 (zh) * 2014-01-16 2017-09-15 Illumina, Inc. 固體支持物上的擴增子製備和測序
US9677132B2 (en) * 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
WO2015131107A1 (en) * 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
CA3219413A1 (en) * 2014-05-16 2015-11-19 Illumina, Inc. Nucleic acid synthesis techniques
US20150361422A1 (en) * 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
CA2953362A1 (en) 2014-06-23 2015-12-30 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
CA2999708A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
CA2964349C (en) 2014-10-14 2023-03-21 Seven Bridges Genomics Inc. Systems and methods for smart tools in sequence pipelines
WO2016112073A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
ES2935860T3 (es) 2015-04-10 2023-03-13 Spatial Transcriptomics Ab Análisis de ácidos nucleicos múltiplex, espacialmente distinguidos de especímenes biológicos
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10275567B2 (en) 2015-05-22 2019-04-30 Seven Bridges Genomics Inc. Systems and methods for haplotyping
CA2987633A1 (en) * 2015-06-02 2016-12-08 Biofire Defense, Llc Sample to sequence
RU2604198C1 (ru) * 2015-06-22 2016-12-10 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Способ получения гетерогенного набора одноцепочечных фрагментов днк для мультиплексного генетического анализа
CN116064738A (zh) * 2015-07-27 2023-05-05 亿明达股份有限公司 核酸序列信息的空间定位
US10793895B2 (en) 2015-08-24 2020-10-06 Seven Bridges Genomics Inc. Systems and methods for epigenetic analysis
US10584380B2 (en) 2015-09-01 2020-03-10 Seven Bridges Genomics Inc. Systems and methods for mitochondrial analysis
US10724110B2 (en) 2015-09-01 2020-07-28 Seven Bridges Genomics Inc. Systems and methods for analyzing viral nucleic acids
AU2016321328A1 (en) * 2015-09-09 2018-04-26 Psomagen, Inc. Method and system for microbiome-derived diagnostics and therapeutics infectious disease and other health conditions associated with antibiotic usage
EP3670671B1 (en) 2015-09-18 2023-07-26 Vanadis Diagnostics Probe set for analyzing a dna sample and method for using the same
US11347704B2 (en) 2015-10-16 2022-05-31 Seven Bridges Genomics Inc. Biological graph or sequence serialization
KR101651817B1 (ko) * 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
CN115044645A (zh) 2015-11-11 2022-09-13 分析生物科学有限公司 Dna文库的高效率构建
US10364468B2 (en) 2016-01-13 2019-07-30 Seven Bridges Genomics Inc. Systems and methods for analyzing circulating tumor DNA
US10460829B2 (en) 2016-01-26 2019-10-29 Seven Bridges Genomics Inc. Systems and methods for encoding genetic variation for a population
US10262102B2 (en) 2016-02-24 2019-04-16 Seven Bridges Genomics Inc. Systems and methods for genotyping with graph reference
US10961568B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked target capture
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
CN109642219B (zh) 2016-08-05 2021-02-26 生物辐射实验室股份有限公司 第二链引导
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US11250931B2 (en) 2016-09-01 2022-02-15 Seven Bridges Genomics Inc. Systems and methods for detecting recombination
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
EP3512947B1 (en) 2016-09-15 2021-10-20 ArcherDX, LLC Methods of nucleic acid sample preparation
EP3518974A4 (en) 2016-09-29 2020-05-27 Myriad Women's Health, Inc. NON-INVASIVE PRENATAL SCREENING USING DYNAMIC ITERATIVE DEEP OPTIMIZATION
WO2018104908A2 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
EP4534692A3 (en) 2017-01-18 2025-06-18 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144216A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
WO2018161019A1 (en) * 2017-03-03 2018-09-07 Counsyl, Inc. Methods for optimizing direct targeted sequencing
EP3602359A4 (en) 2017-03-24 2021-01-06 Myriad Women's Health, Inc. NUMBER OF COPIES VARIANT CALLER
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS
CN111542532B (zh) * 2017-10-04 2024-03-15 生捷科技控股公司 酶法合成寡核苷酸的方法和体系
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3749779B1 (en) * 2018-02-07 2024-12-25 Tecan Genomics, Inc. Library preparation
EP3781585A4 (en) 2018-04-17 2022-01-26 The General Hospital Corporation SENSITIVE IN VITRO ASSAYS FOR SUBSTRATE AND SITE PREFERENCES OF NUCLEIC ACID BINDERS, MODIFIERS AND CLEAVATORS
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
WO2020039261A1 (en) 2018-08-23 2020-02-27 Boreal Genomics, Inc. Linked target capture and ligation
WO2020076914A1 (en) * 2018-10-09 2020-04-16 Academia Sinica Digital polymerase chain reaction method for detecting nucleic acids in samples
CN119614683A (zh) 2018-12-10 2025-03-14 10X基因组学有限公司 生成用于空间分析的捕获探针
WO2020118655A1 (zh) 2018-12-14 2020-06-18 深圳华大生命科学研究院 核酸合成装置和纯化装置、其用途及核酸合成方法和纯化方法
CN112654714B (zh) * 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
WO2020141464A1 (en) 2019-01-03 2020-07-09 Boreal Genomics, Inc. Linked target capture
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3972611A4 (en) * 2019-05-21 2023-06-14 Academia Sinica METHOD FOR AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACID (RNA) FRAGMENTS
US11639521B2 (en) * 2019-06-03 2023-05-02 The Chinese University Of Hong Kong Method for determining the copy number of a tandem repeat sequence
CN117535379A (zh) * 2019-08-09 2024-02-09 深圳市真迈生物科技有限公司 一种捕获核酸分子的方法及其应用
US11795495B1 (en) * 2019-10-02 2023-10-24 FOXO Labs Inc. Machine learned epigenetic status estimator
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
EP4534690A3 (en) * 2020-01-31 2025-07-23 Agilent Technologies, Inc. Systems and methods for targeted nucleic acid capture
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
DK3969613T3 (da) * 2020-02-26 2024-01-08 Illumina Inc Kits til genotypebestemmelse
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150126A4 (en) * 2020-05-12 2024-06-19 Singular Genomics Systems, Inc. NUCLEIC ACID AMPLIFICATION PROCESSES
CN111676276A (zh) * 2020-07-13 2020-09-18 湖北伯远合成生物科技有限公司 一种快速精准确定基因编辑突变情况的方法及其应用
EP4153606A4 (en) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. METHODS FOR SEQUENCING COMPLEMENTARY POLYNUCLEOTIDES
WO2022026905A1 (en) * 2020-07-31 2022-02-03 Arc Bio, Llc Systems, methods, and media for determining relative quality of oligonucleotide preparations
EP4211239A4 (en) * 2020-09-08 2024-09-11 Seqwell, Inc. Sequencing oligonucleotides and methods of use thereof
RU2748380C1 (ru) * 2020-11-10 2021-05-25 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Набор олигонуклеотидных праймеров и способ генотипирования однонуклеотидного полиморфизма rs8065080 в гене TRPV1 человека
CN112837749B (zh) * 2021-02-01 2021-11-26 北京百奥纳芯生物科技有限公司 一种癌症筛查用基因芯片探针的优选方法
US11578320B2 (en) * 2021-04-27 2023-02-14 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
EP4373967A4 (en) * 2021-07-20 2025-06-11 Freenome Holdings, Inc. Compositions and methods for improved resolution of 5-hydroxymethylated cytosine in nucleic acid sequencing
CN114621307B (zh) * 2022-04-12 2024-12-13 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
WO2025072683A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification

Family Cites Families (271)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
AU4128089A (en) 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US7223833B1 (en) 1991-05-24 2007-05-29 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
DE69329800D1 (de) 1992-04-27 2001-02-01 Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CH686982A5 (fr) 1993-12-16 1996-08-15 Maurice Stroun Méthode pour le diagnostic de cancers.
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5789206A (en) 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
NO954667D0 (no) 1995-11-17 1995-11-17 Dagfinn Oegreid Fremgangsmåte til deteksjon av Ki-ras mutasjoner
EP0929694A4 (en) 1996-03-15 2002-05-02 Penn State Res Found DETECTION OF TUMOR-RELATED EXTRACELLULAR NUCLEIC ACID USING PLASMA OR BLOOD SERUM USING NUCLEIC ACID AMPLIFICATION TESTS
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
SE9602829D0 (sv) 1996-07-19 1996-07-19 Yngve Johnsson Förfarande och anordning för betalningsmedelshantering jämte betalningsmedel
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6262242B1 (en) 1997-01-30 2001-07-17 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
US6482795B1 (en) 1997-01-30 2002-11-19 Myriad Genetics, Inc. Tumor suppressor designated TS10q23.3
EP0972081B1 (en) * 1997-04-01 2007-06-13 Solexa Ltd. Method of nucleic acid amplification
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JPH10341047A (ja) 1997-06-06 1998-12-22 Sony Corp 磁気トンネル素子
CA2295324C (en) 1997-07-07 2012-12-18 Andrew Griffiths In vitro sorting method
US6008002A (en) 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
EP1062515B1 (en) 1998-02-12 2009-11-25 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells
JPH11355695A (ja) 1998-06-10 1999-12-24 Sony Corp 映像信号処理装置
DE19833738A1 (de) 1998-07-27 2000-02-03 Michael Giesing Verfahren zur Isolierung von Krebszellen aus zellhaltigen Körperflüssigkeiten sowie Sets zur Durchführung dieses Verfahrens
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6864052B1 (en) * 1999-01-06 2005-03-08 Callida Genomics, Inc. Enhanced sequencing by hybridization using pools of probes
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US9534254B1 (en) 1999-02-02 2017-01-03 Abbott Molecular Inc. Patient stratification for cancer therapy based on genomic DNA microarray analysis
US6395481B1 (en) 1999-02-16 2002-05-28 Arch Development Corp. Methods for detection of promoter polymorphism in a UGT gene promoter
US6410231B1 (en) 1999-02-26 2002-06-25 Incyte Genomics, Inc. SNP detection
US7324926B2 (en) 1999-04-09 2008-01-29 Whitehead Institute For Biomedical Research Methods for predicting chemosensitivity or chemoresistance
US20010034023A1 (en) 1999-04-26 2001-10-25 Stanton Vincent P. Gene sequence variations with utility in determining the treatment of disease, in genes relating to drug processing
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US6410243B1 (en) 1999-09-01 2002-06-25 Whitehead Institute For Biomedical Research Chromosome-wide analysis of protein-DNA interactions
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
EP1248832A4 (en) 2000-01-21 2004-07-07 Variagenics Inc IDENTIFICATION OF THE GENETIC COMPONENTS OF A MEDICINE REACTION
JP2004514112A (ja) 2000-01-26 2004-05-13 ヴェンタナ　メディカル　システムズ　インコーポレイテッド 個別化した医薬に対するアッセイ法の開発システム
WO2001064864A2 (en) 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
EP1995330A1 (en) 2000-03-01 2008-11-26 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US20020120409A1 (en) 2000-05-19 2002-08-29 Affymetrix, Inc. Methods for gene expression analysis
WO2001096382A2 (en) 2000-06-15 2001-12-20 Prokaria Ehf. Thermostable cellulase
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
AU2001290879A1 (en) 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
US7105293B2 (en) 2000-09-19 2006-09-12 Whitehead Institute For Biomedical Research Genetic markers for tumors
EP1366192B8 (en) 2000-10-24 2008-10-29 The Board of Trustees of the Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20050021240A1 (en) 2000-11-02 2005-01-27 Epigenomics Ag Systems, methods and computer program products for guiding selection of a therapeutic treatment regimen based on the methylation status of the DNA
CA2427802A1 (en) 2000-11-03 2002-05-10 Dana-Farber Cancer Institute Methods and compositions for the diagnosis of cancer susceptibilites and defective dna repair mechanisms and treatment thereof
EP1207209A3 (en) 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
CN102220414A (zh) 2000-12-01 2011-10-19 应答遗传公司 测定表皮生长因子受体和HER2-neu的基因表达及其水平与存活率之间相关性的方法
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US20020115073A1 (en) 2001-02-16 2002-08-22 Nickolas Papadopoulos Genome-based personalized medicine
US7268167B2 (en) 2001-02-23 2007-09-11 Japan Science And Technology Agency Process for producing emulsion and microcapsules and apparatus therefor
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20040110193A1 (en) 2001-07-31 2004-06-10 Gene Logic, Inc. Methods for classification of biological data
US20030165940A1 (en) 2001-12-06 2003-09-04 The Johns Hopkins University Disease detection by digital protein truncation assays
WO2003054149A2 (en) 2001-12-07 2003-07-03 University Of Massachusetts Targeted genetic risk-stratification using microarrays
US6949342B2 (en) 2001-12-21 2005-09-27 Whitehead Institute For Biomedical Research Prostate cancer diagnosis and outcome prediction by expression analysis
AU2002353228B2 (en) 2001-12-21 2008-09-11 The Welcome Trust Genes
US7730063B2 (en) 2002-12-10 2010-06-01 Asset Trust, Inc. Personalized medicine service
ATE441107T1 (de) 2002-03-01 2009-09-15 Siemens Healthcare Diagnostics Assays zur überwachung von krebspatienten auf grundlage der spiegel des analyten für die extrazelluläre domäne (ecd) des epidermalen wachstumsfaktor-rezeptors (egfr), allein oder in kombination mit anderen analyten, in proben von kírperflüssigkeiten
WO2003078450A2 (en) 2002-03-11 2003-09-25 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7427480B2 (en) 2002-03-26 2008-09-23 Perlegen Sciences, Inc. Life sciences business systems and methods
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
WO2003102216A2 (en) * 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US9388459B2 (en) * 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
TWI266907B (en) 2002-07-24 2006-11-21 Nitto Denko Corp Polarizing device, optical thin film using the same, and image display device using the same
JP2006500034A (ja) 2002-09-19 2006-01-05 アプレラ コーポレイション 標的を検出するための方法および組成物
WO2004027054A1 (en) 2002-09-20 2004-04-01 Prokaria Ehf. Thermostable rna ligase from thermus phage
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
WO2004046386A1 (en) 2002-11-15 2004-06-03 Genomic Health, Inc. Gene expression profiling of egfr positive cancer
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
AU2003302770B2 (en) 2002-12-20 2007-07-12 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
US20040137539A1 (en) 2003-01-10 2004-07-15 Bradford Sherry A. Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies
CA2728746C (en) 2003-01-29 2018-01-16 454 Corporation Methods of amplifying and sequencing nucleic acids
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20080044813A1 (en) 2003-03-20 2008-02-21 Kristian Jansson Amp Ligation Assay (Ala)
EP3261006A1 (en) 2003-04-09 2017-12-27 Omicia Inc. Methods of selection, reporting and analysis of genetic markers using broad based genetic profiling applications
MXPA05011404A (es) 2003-04-25 2006-05-31 Johnson & Johnson Conservacion del arn en una muestra biologica.
US20050064455A1 (en) 2003-05-28 2005-03-24 Baker Joffre B. Gene expression markers for predicting response to chemotherapy
RU2392324C2 (ru) * 2003-09-18 2010-06-20 Симфоген А/С Способ связывания интересующих последовательностей
US20050153317A1 (en) 2003-10-24 2005-07-14 Metamorphix, Inc. Methods and systems for inferring traits to breed and manage non-beef livestock
AU2004286352A1 (en) 2003-10-28 2005-05-12 Epoch Biosciences, Inc. Fluorescent probes for DNA detection by hybridization with improved sensitivity and low background
WO2005044091A2 (en) 2003-11-05 2005-05-19 Board Of Regents, The University Of Texas System Diagnostic and therapeutic methods and compositions involving pten and breast cancer
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US20050181377A1 (en) 2004-02-13 2005-08-18 Markovic Svetomir N. Targeted cancer therapy
US20060195266A1 (en) 2005-02-25 2006-08-31 Yeatman Timothy J Methods for predicting cancer outcome and gene signatures for use therein
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
JP2007527241A (ja) 2004-03-01 2007-09-27 ユニバーシティ オブ シカゴ 上皮細胞成長因子受容体遺伝子プロモーターにおける多型
JP2007530048A (ja) 2004-03-24 2007-11-01 アプレラ コーポレイション 標的ポリヌクレオチドを検出するためのコード化反応およびデコード反応
ES2741547T3 (es) 2004-03-31 2020-02-11 Massachusetts Gen Hospital Método para determinar la respuesta del cáncer a tratamientos dirigidos al receptor del factor de crecimiento epidérmico
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
CA2563074C (en) 2004-04-09 2014-05-20 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
WO2005108583A1 (en) 2004-05-06 2005-11-17 Prokaria Ehf. Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
WO2005118876A2 (en) 2004-06-04 2005-12-15 Genentech, Inc. Egfr mutations
US7329495B2 (en) 2004-06-09 2008-02-12 Board Of Regents, The University Of Texas System Mutations in KIT confer imatinib resistance in gastrointestinal stromal tumors
US20060115827A1 (en) 2004-07-01 2006-06-01 University Of Southern California Genetic markers for predicting disease and treatment outcome
EP1784646B1 (en) 2004-08-11 2012-06-13 Albert Einstein College Of Medicine Of Yeshiva University Method for identifying metastasis in motile cells
ES2529204T3 (es) 2004-10-18 2015-02-17 Brandeis University Métodos para la amplificación de ácido nucleico
KR20070073917A (ko) 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 개선된 증폭을 위한 핵산 복구
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US20060278241A1 (en) 2004-12-14 2006-12-14 Gualberto Ruano Physiogenomic method for predicting clinical outcomes of treatments in patients
US20060184489A1 (en) 2004-12-17 2006-08-17 General Electric Company Genetic knowledgebase creation for personalized analysis of medical conditions
WO2006079034A2 (en) 2005-01-21 2006-07-27 Gene Express, Inc. Business methods for assessing nucleic acids
US7442507B2 (en) 2005-01-24 2008-10-28 New York University School Of Medicine Methods for detecting circulating mutant BRAF DNA
AU2006207933B2 (en) 2005-01-28 2010-11-18 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
BRPI0609615A2 (pt) 2005-04-01 2010-04-27 Amgen Inc métodos de predição da eficácia de tratamento de agente de ligação especìfica a egfr em tratamento de cáncer relacionado com egfr num sujeito, de tratamento do mesmo e de determinação da eficácia de tratamento em paciente
PL1712639T3 (pl) 2005-04-06 2009-02-27 Maurice Stroun Sposób diagnozowania nowotworu przez wykrywanie krążącego DNA i RNA
JP2008535508A (ja) 2005-04-14 2008-09-04 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 腫瘍組織における増大したコピー数のｅｇｆｒ遺伝子に基づく抗ｅｇｆｒ抗体療法
JP4547301B2 (ja) 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス及び分析システム
US20070020657A1 (en) 2005-05-20 2007-01-25 Grebe Stefan K Methods for detecting circulating tumor cells
WO2006128463A2 (en) 2005-05-31 2006-12-07 Dako Denmark A/S Compositions and methods for predicting outcome of treatment
PL1913157T5 (pl) 2005-06-28 2017-09-29 Genentech Inc Mutacje EGFR i KRAS do prognozowania reakcji pacjenta na leczenie inhibitorem EGFR
GB0514909D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1943339A2 (en) 2005-09-16 2008-07-16 454 Life Sciences Corporation Cdna library preparation
CA2623445A1 (en) 2005-09-21 2007-03-29 Ccc Diagnostics, Llc Comprehensive diagnostic testing procedures for personalized anticancer chemotherapy (pac)
US20070172844A1 (en) 2005-09-28 2007-07-26 University Of South Florida Individualized cancer treatments
PL1948816T3 (pl) 2005-10-24 2012-07-31 Univ Johns Hopkins Ulepszone sposoby beamingu
WO2007073171A2 (en) 2005-12-22 2007-06-28 Keygene N.V. Improved strategies for transcript profiling using high throughput sequencing technologies
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
WO2007091228A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A liquid bridge and system
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
EP1984735B1 (en) 2006-02-16 2010-10-06 Ventana Medical Systems, Inc. Reagents and methods for cancer prognosis and pathological staging
WO2007100243A1 (en) 2006-03-01 2007-09-07 Keygene N.V. High throughput sequence-based detection of snps using ligation assays
US8165367B2 (en) 2006-03-08 2012-04-24 Olympus Medical Systems Corp. Medical image processing apparatus and medical image processing method having three-dimensional model estimating
WO2007106432A2 (en) 2006-03-10 2007-09-20 George Mason Intellectual Properties, Inc. Egf receptor phosphorylation status for disease treatment
US7908091B2 (en) 2006-03-17 2011-03-15 Prometheus Laboratories Inc. Methods of predicting and monitoring tyrosine kinase inhibitor therapy
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
KR20090008290A (ko) 2006-03-27 2009-01-21 글로브이뮨 Ｒａｓ 돌연변이와 그에 관련된 조성물 및 방법
TWI395754B (zh) 2006-04-24 2013-05-11 Amgen Inc 人類化之ｃ-ｋｉｔ抗體
WO2007133506A2 (en) 2006-05-09 2007-11-22 University Of Louisville Research Foundation , Inc. Personalized medicine management software
US20080003142A1 (en) 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US8700335B2 (en) 2006-05-18 2014-04-15 Caris Mpi, Inc. System and method for determining individualized medical intervention for a disease state
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
US20080124721A1 (en) 2006-06-14 2008-05-29 Martin Fuchs Analysis of rare cell-enriched samples
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US20080065411A1 (en) 2006-09-08 2008-03-13 Diaceutics Method and system for developing a personalized medicine business plan
SG174826A1 (en) 2006-09-19 2011-10-28 Novartis Ag Biomarkers of target modulation, efficacy, diagnosis and/or prognosis for raf inhibitors
WO2008038259A1 (en) 2006-09-28 2008-04-03 Stokes Bio Limited A qpcr analysis apparatus
EP2076609A1 (en) 2006-10-10 2009-07-08 Illumina Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
CN101641451A (zh) 2006-10-27 2010-02-03 解码遗传学私营有限责任公司 chr8q24.21上的癌症易感性变体
EP2089055A4 (en) 2006-11-01 2011-02-02 George Mason Intellectual Prop METHOD FOR THE DETECTION AND CONTROL OF CANCER
US20100196889A1 (en) 2006-11-13 2010-08-05 Bankaitis-Davis Danute M Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer
US20080131887A1 (en) 2006-11-30 2008-06-05 Stephan Dietrich A Genetic Analysis Systems and Methods
US20100143935A1 (en) 2006-12-01 2010-06-10 Apocell, Inc. c-KIT Phosphorylation in Cancer
EP4134667A1 (en) 2006-12-14 2023-02-15 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20080177608A1 (en) 2007-01-19 2008-07-24 Diaceutics Business method for enabling personalized medicine
US9556491B2 (en) 2007-02-26 2017-01-31 John Wayne Cancer Institute Utility of B-RAF DNA mutation in diagnosis and treatment of cancer
EP2129397B1 (en) 2007-03-02 2018-10-31 Amgen, Inc. Methods and compositions for treating tumor diseases
AR065686A1 (es) 2007-03-13 2009-06-24 Amgen Inc Mutaciones de k- ras y b- raf y terapia con anticuerpos anti- egfr
PL2121989T5 (pl) 2007-03-13 2023-03-06 Amgen Inc. Mutacje K-RAS oraz terapia przeciwciałem anty-EGFR
CA2680591A1 (en) 2007-03-15 2008-09-25 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
CA2680593A1 (en) 2007-03-19 2008-09-25 Cold Spring Harbor Laboratory Identification of genetic alterations that modulate drug sensitivity in cancer treatments
US20080255243A1 (en) 2007-04-13 2008-10-16 Petricoin Emanuel F Stat3 as a theranostic indicator
EP2137535B1 (en) 2007-04-13 2015-06-03 Dana-Farber Cancer Institute, Inc. Receptor tyrosine kinase profiling
WO2008147879A1 (en) 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
WO2008148072A2 (en) 2007-05-24 2008-12-04 The Brigham And Women's Hospital, Inc. Disease-associated genetic variations and methods for obtaining and using same
CA2691364C (en) 2007-06-19 2020-06-16 Stratos Genomics, Inc. High throughput nucleic acid sequencing by expansion
GB0712882D0 (en) * 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
JP5352585B2 (ja) 2007-07-13 2013-11-27 ネステク ソシエテ アノニム 抗体アレイを用いた肺癌治療のための薬剤選択
EP2183693B2 (en) 2007-07-23 2018-11-14 The Chinese University of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
WO2009021019A1 (en) 2007-08-06 2009-02-12 Health Research Inc. Methods for analysis of pdef and survivin as interconnected cancer biomarkers and targets for personalized medicine
US20090062138A1 (en) 2007-08-31 2009-03-05 Curry Bo U Array-based method for performing SNP analysis
US20100173294A1 (en) 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
US20110212991A1 (en) 2007-09-11 2011-09-01 Roche Molecular Systems, Inc. Diagnostic Test for Susceptibility to B-RAF Kinase Inhibitors
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US20090209436A1 (en) * 2007-09-17 2009-08-20 Twof, Inc. Hydrogel labeled primer extension method for microarrays
EA201000427A1 (ru) 2007-10-04 2010-10-29 Хэлсион Молекулар Секвенирование нуклеиново-кислотных полимеров с использованием электронной микроскопии
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20090264298A1 (en) 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
EP2063132A1 (de) * 2007-11-24 2009-05-27 Festo AG & Co. KG Linearantriebsvorrichtung
US8530197B2 (en) 2008-01-09 2013-09-10 Applied Biosystems, Llc Method of making a paired tag library for nucleic acid sequencing
EP2245191A1 (en) 2008-01-17 2010-11-03 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
US11051733B2 (en) 2008-01-18 2021-07-06 Wake Forest University Health Sciences Isolating and purifying cells for therapy
ES2703363T3 (es) 2008-02-01 2019-03-08 Massachusetts Gen Hospital Uso de microvesículas en el diagnóstico y pronóstico de tumores cerebrales
US20100029498A1 (en) 2008-02-04 2010-02-04 Andreas Gnirke Selection of nucleic acids by solution hybridization to oligonucleotide baits
NZ587903A (en) 2008-02-14 2013-05-31 Decode Genetics Ehf Susceptibility for lung cancer using the polymorphic marker rs1051730
WO2009102957A2 (en) 2008-02-14 2009-08-20 The Johns Hopkins University Methods to connect gene set expression profiles to drug sensitivity
CA2923248A1 (en) 2008-02-25 2009-09-03 Nestec S.A. Methods for detecting truncated receptors
US20090226975A1 (en) * 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
US8067178B2 (en) 2008-03-14 2011-11-29 Genomic Health, Inc. Gene expression markers for prediction of patient response to chemotherapy
US20090307180A1 (en) 2008-03-19 2009-12-10 Brandon Colby Genetic analysis
US7842248B2 (en) 2008-06-20 2010-11-30 Silverbrook Research Pty Ltd Microfluidic system comprising microfluidic pump, mixer or valve
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
JP2010041985A (ja) * 2008-08-18 2010-02-25 Hitachi Plant Technologies Ltd 核酸配列増幅方法、核酸配列の検出方法及び核酸増幅・検出用基板
GB2467691A (en) 2008-09-05 2010-08-11 Aueon Inc Methods for stratifying and annotating cancer drug treatment options
GB2477053B (en) 2008-09-23 2013-11-13 Quantalife Inc Droplet-based assay system
EP2347009A4 (en) 2008-10-14 2012-05-30 Caris Mpi Inc GENE AND GENE EXPRESSED TARGET PROTEINS FOR THE PRESENTATION OF BIOMARKERS AND SIGNATURES BY TUMOR TYPE
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010059323A2 (en) 2008-11-18 2010-05-27 Life Technologies Corporation Sequence amplification with target primers
US10262103B2 (en) 2008-11-18 2019-04-16 Raphael LEHRER Individualized cancer treatment
WO2010077324A2 (en) 2008-12-17 2010-07-08 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US8790873B2 (en) 2009-01-16 2014-07-29 Affymetrix, Inc. DNA ligation on RNA template
SG173105A1 (en) 2009-02-16 2011-09-29 Epict Technologies Corp Template-independent ligation of single-stranded dna
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
CN102460155B (zh) 2009-04-29 2015-03-25 考利达基因组股份有限公司 用于关于参考多核苷酸序列标注样本多核苷酸序列中的变异的方法和系统
CA2770389C (en) 2009-08-25 2015-12-29 Illumina, Inc. Methods for selecting and amplifying polynucleotides
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
WO2011050981A2 (en) * 2009-10-30 2011-05-05 Roche Diagnostics Gmbh Method for detecting balanced chromosomal aberrations in a genome
US20110117559A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
EP2504448B1 (en) 2009-11-25 2016-10-19 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
US20110157322A1 (en) 2009-12-31 2011-06-30 Broadcom Corporation Controlling a pixel array to support an adaptable light manipulator
DK2539450T3 (da) 2010-02-25 2016-05-30 Advanced Liquid Logic Inc Fremgangsmåde til fremstilling af nukleinsyrebiblioteker
WO2011123749A1 (en) 2010-04-01 2011-10-06 New England Biolabs, Inc. Compositions and methods for adenylating oligonucleotides
WO2011140510A2 (en) 2010-05-06 2011-11-10 Bioo Scientific Corporation Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing
US20110299645A1 (en) 2010-06-07 2011-12-08 Korea Hydro & Nuclear Power Co., Ltd. Breeding Nuclear Fuel Mixture Using Metallic Thorium
US20120003657A1 (en) 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
NZ608313A (en) 2010-09-24 2013-12-20 Univ Leland Stanford Junior Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
EP3564392B1 (en) 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
SG191818A1 (en) 2010-12-30 2013-08-30 Foundation Medicine Inc Optimization of multigene analysis of tumor samples
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
DK3246416T3 (da) 2011-04-15 2024-09-02 Univ Johns Hopkins Sikkert sekventeringssystem
US10865409B2 (en) 2011-09-07 2020-12-15 X-Chem, Inc. Methods for tagging DNA-encoded libraries
ES2729554T3 (es) 2011-10-21 2019-11-04 Chronix Biomedical Biomarcadores de ácidos nucleicos en circulación asociados al cáncer colorrectal
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
SG10201906331TA (en) 2012-01-27 2019-08-27 Univ Leland Stanford Junior Methods for profiling and quantitating cell-free rna
EP2812441A1 (en) 2012-02-06 2014-12-17 Wisconsin Alumni Research Foundation Nucleic acid ligation method
WO2013173472A1 (en) 2012-05-15 2013-11-21 Predictive Biosciences, Inc. Methods of assessing chromosomal instabilities
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US9816120B2 (en) 2013-01-09 2017-11-14 The Penn State Research Foundation Low sequence bias single-stranded DNA ligation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140287937A1 (en) 2013-02-21 2014-09-25 Toma Biosciences, Inc. Methods for assessing cancer
EP2959016A4 (en) 2013-02-21 2016-10-12 Toma Biosciences Inc METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
ES2707744T3 (es) 2013-12-11 2019-04-04 Accuragen Holdings Ltd Métodos para detectar variantes de secuencia raras
US20190050530A1 (en) 2016-02-09 2019-02-14 Toma Biosciences, Inc. Systems and Methods for Analyzing Nucleic Acids

Also Published As

Publication number Publication date
EP2619329A4 (en) 2014-03-05
IL225109A (en) 2017-05-29
JP2013544498A (ja) 2013-12-19
JP5986572B2 (ja) 2016-09-06
US20190024141A1 (en) 2019-01-24
CA2810931A1 (en) 2012-03-29
AU2011305445A1 (en) 2013-04-04
EP3572528A1 (en) 2019-11-27
AU2011305445B2 (en) 2017-03-16
IN2013MN00522A (forum.php) 2015-05-29
US20120157322A1 (en) 2012-06-21
RU2565550C2 (ru) 2015-10-20
KR20130113447A (ko) 2013-10-15
US10072283B2 (en) 2018-09-11
MX346956B (es) 2017-04-06
MX2013003349A (es) 2013-09-13
CN103228798A (zh) 2013-07-31
WO2012040387A1 (en) 2012-03-29
US20150017635A1 (en) 2015-01-15
CN103228798B (zh) 2015-12-09
NZ608313A (en) 2013-12-20
US9309556B2 (en) 2016-04-12
RU2013118722A (ru) 2014-10-27
EP2619329B1 (en) 2019-05-22
EP2619329A1 (en) 2013-07-31

Similar Documents

Publication Publication Date Title
CA2810931C (en) Direct capture, amplification and sequencing of target dna using immobilized primers
US20220042090A1 (en) PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)
CN110036117B (zh) 通过多联短dna片段增加单分子测序的处理量的方法
US9574234B2 (en) Solid-phase clonal amplification and related methods
US20220364169A1 (en) Sequencing method for genomic rearrangement detection
US10465241B2 (en) High resolution STR analysis using next generation sequencing
US11898202B2 (en) Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples
US20250146063A1 (en) Method for high-throughput detection of target nucleotide sequences
EP4332235A1 (en) Highly sensitive methods for accurate parallel quantification of variant nucleic acids

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20131017