CA2807397C - Method of producing and purifying an active soluble sialyltransferase - Google Patents
Method of producing and purifying an active soluble sialyltransferase Download PDFInfo
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- CA2807397C CA2807397C CA2807397A CA2807397A CA2807397C CA 2807397 C CA2807397 C CA 2807397C CA 2807397 A CA2807397 A CA 2807397A CA 2807397 A CA2807397 A CA 2807397A CA 2807397 C CA2807397 C CA 2807397C
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- sialyltransferase
- polypeptide
- chromatography
- resin
- st6galnaci
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| EP20100171626 EP2415779B1 (en) | 2010-08-02 | 2010-08-02 | Method of producing and purifying an active soluble sialyltransferase |
| EP10171626.4 | 2010-08-02 | ||
| PCT/EP2011/063291 WO2012016984A1 (en) | 2010-08-02 | 2011-08-02 | Method of producing and purifying an active soluble sialyltransferase |
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| CA2807397A1 CA2807397A1 (en) | 2012-02-09 |
| CA2807397C true CA2807397C (en) | 2019-03-19 |
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| CA2807397A Active CA2807397C (en) | 2010-08-02 | 2011-08-02 | Method of producing and purifying an active soluble sialyltransferase |
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| Country | Link |
|---|---|
| US (1) | US9222081B2 (OSRAM) |
| EP (2) | EP2415779B1 (OSRAM) |
| JP (1) | JP5984267B2 (OSRAM) |
| CN (1) | CN103154017B (OSRAM) |
| CA (1) | CA2807397C (OSRAM) |
| ES (1) | ES2534936T3 (OSRAM) |
| MX (1) | MX2013001447A (OSRAM) |
| PT (1) | PT2415779E (OSRAM) |
| WO (1) | WO2012016984A1 (OSRAM) |
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| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| JP2016513245A (ja) * | 2013-02-06 | 2016-05-12 | エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ | タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面 |
| HUE038741T2 (hu) | 2013-05-06 | 2018-11-28 | Sanofi Sa | Folyamatos többlépéses eljárás antitestek tisztítására |
| KR20170132293A (ko) * | 2015-03-31 | 2017-12-01 | 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 | 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도 |
| KR101667677B1 (ko) * | 2015-05-29 | 2016-10-19 | 한국생명공학연구원 | 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법 |
| EP3559250A1 (en) | 2016-12-21 | 2019-10-30 | H. Hoffnabb-La Roche Ag | Re-use of enzymes in in vitro glycoengineering of antibodies |
| AU2017381657B2 (en) | 2016-12-21 | 2020-07-23 | F. Hoffmann-La Roche Ag | Method for in vitro glycoengineering of antibodies |
| EP3678687B1 (en) | 2017-09-04 | 2022-08-17 | 89Bio Ltd. | Mutant fgf-21 peptide conjugates and uses thereof |
| WO2019063849A1 (en) * | 2017-12-20 | 2019-04-04 | Dsm Ip Assets B.V. | PURIFICATION OF A POLYPEPTIDE OF INTEREST |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| KR102362777B1 (ko) * | 2018-03-27 | 2022-02-15 | 주식회사 녹십자 | 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법 |
| KR102140531B1 (ko) * | 2018-08-07 | 2020-08-04 | (주)휴온스 | Gly-Tβ4의 제조방법 |
| EP3613486B1 (de) * | 2018-08-24 | 2020-10-07 | UGA Biopharma GmbH | Verfahren und anlage zur reinigung von epo und/oder einem epo-derivat |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
| CN119912505A (zh) * | 2025-04-02 | 2025-05-02 | 浙江熙正霖生物科技有限公司 | 一种从发酵液中提纯唾液酸的方法 |
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| US5032519A (en) * | 1989-10-24 | 1991-07-16 | The Regents Of The Univ. Of California | Method for producing secretable glycosyltransferases and other Golgi processing enzymes |
| EP0737745A4 (en) | 1993-12-24 | 1999-06-16 | Rikagaku Kenkyusho | INNOVATIVE SYNTHETASE FOR SUGAR CHAINS AND METHOD FOR THEIR PRODUCTION |
| US6280989B1 (en) * | 1999-06-17 | 2001-08-28 | Dmitri Kapitonov | Sialyltransferases |
| ES2561985T3 (es) | 2001-10-10 | 2016-03-01 | Ratiopharm Gmbh | Remodelación y glicoconjugación de anticuerpos |
| CN1897812B (zh) * | 2003-12-03 | 2011-08-17 | 生物种属学股份公司 | 糖聚乙二醇化的粒细胞集落刺激因子 |
| WO2005121332A2 (en) | 2004-06-03 | 2005-12-22 | Neose Technologies, Inc. | Truncated st6galnaci polypeptides and nucleic acids |
| KR20080021590A (ko) | 2005-03-24 | 2008-03-07 | 네오스 테크놀로지스, 인크. | 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현 |
| US20060246544A1 (en) | 2005-03-30 | 2006-11-02 | Neose Technologies,Inc. | Manufacturing process for the production of peptides grown in insect cell lines |
| WO2007056524A2 (en) | 2005-11-09 | 2007-05-18 | Neose Technologies, Inc. | St3gal-1/st6galnac-1 chimeras |
| JP2010505874A (ja) * | 2006-10-03 | 2010-02-25 | ノヴォ ノルディスク アー／エス | ポリペプチドコンジュゲートの精製方法 |
| US20080207487A1 (en) | 2006-11-02 | 2008-08-28 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
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2010
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2011
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- 2011-08-02 US US13/813,804 patent/US9222081B2/en active Active
- 2011-08-02 WO PCT/EP2011/063291 patent/WO2012016984A1/en not_active Ceased
- 2011-08-02 JP JP2013522234A patent/JP5984267B2/ja active Active
- 2011-08-02 MX MX2013001447A patent/MX2013001447A/es unknown
- 2011-08-02 CA CA2807397A patent/CA2807397C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012016984A1 (en) | 2012-02-09 |
| MX2013001447A (es) | 2013-11-20 |
| CA2807397A1 (en) | 2012-02-09 |
| US20140057316A1 (en) | 2014-02-27 |
| EP2415779A1 (en) | 2012-02-08 |
| EP2415779B1 (en) | 2015-01-21 |
| CN103154017A (zh) | 2013-06-12 |
| PT2415779E (pt) | 2015-05-13 |
| ES2534936T3 (es) | 2015-04-30 |
| US9222081B2 (en) | 2015-12-29 |
| EP2601207A1 (en) | 2013-06-12 |
| JP5984267B2 (ja) | 2016-09-06 |
| EP2601207B1 (en) | 2016-12-28 |
| CN103154017B (zh) | 2016-02-03 |
| JP2013535207A (ja) | 2013-09-12 |
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