CN103154017B - 生产和纯化活性可溶唾液酸转移酶的方法 - Google Patents

生产和纯化活性可溶唾液酸转移酶的方法 Download PDF

Info

Publication number
CN103154017B
CN103154017B CN201180047627.7A CN201180047627A CN103154017B CN 103154017 B CN103154017 B CN 103154017B CN 201180047627 A CN201180047627 A CN 201180047627A CN 103154017 B CN103154017 B CN 103154017B
Authority
CN
China
Prior art keywords
chromatography
sialyltransferase
st6galnaci
exchange chromatography
resin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201180047627.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN103154017A (zh
Inventor
A·安格曼
C·谢克曼
K·施密特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ratiopharm GmbH
Original Assignee
Ratiopharm GmbH
Biogenerix GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ratiopharm GmbH, Biogenerix GmbH filed Critical Ratiopharm GmbH
Publication of CN103154017A publication Critical patent/CN103154017A/zh
Application granted granted Critical
Publication of CN103154017B publication Critical patent/CN103154017B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180047627.7A 2010-08-02 2011-08-02 生产和纯化活性可溶唾液酸转移酶的方法 Expired - Fee Related CN103154017B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
EP10171626.4 2010-08-02
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (2)

Publication Number Publication Date
CN103154017A CN103154017A (zh) 2013-06-12
CN103154017B true CN103154017B (zh) 2016-02-03

Family

ID=42736163

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180047627.7A Expired - Fee Related CN103154017B (zh) 2010-08-02 2011-08-02 生产和纯化活性可溶唾液酸转移酶的方法

Country Status (9)

Country Link
US (1) US9222081B2 (OSRAM)
EP (2) EP2415779B1 (OSRAM)
JP (1) JP5984267B2 (OSRAM)
CN (1) CN103154017B (OSRAM)
CA (1) CA2807397C (OSRAM)
ES (1) ES2534936T3 (OSRAM)
MX (1) MX2013001447A (OSRAM)
PT (1) PT2415779E (OSRAM)
WO (1) WO2012016984A1 (OSRAM)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
JP2016513245A (ja) * 2013-02-06 2016-05-12 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面
HUE038741T2 (hu) 2013-05-06 2018-11-28 Sanofi Sa Folyamatos többlépéses eljárás antitestek tisztítására
KR20170132293A (ko) * 2015-03-31 2017-12-01 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
EP3559250A1 (en) 2016-12-21 2019-10-30 H. Hoffnabb-La Roche Ag Re-use of enzymes in in vitro glycoengineering of antibodies
AU2017381657B2 (en) 2016-12-21 2020-07-23 F. Hoffmann-La Roche Ag Method for in vitro glycoengineering of antibodies
EP3678687B1 (en) 2017-09-04 2022-08-17 89Bio Ltd. Mutant fgf-21 peptide conjugates and uses thereof
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
EP3613486B1 (de) * 2018-08-24 2020-10-07 UGA Biopharma GmbH Verfahren und anlage zur reinigung von epo und/oder einem epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
WO2005121332A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated st6galnaci polypeptides and nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
EP0737745A4 (en) 1993-12-24 1999-06-16 Rikagaku Kenkyusho INNOVATIVE SYNTHETASE FOR SUGAR CHAINS AND METHOD FOR THEIR PRODUCTION
ES2561985T3 (es) 2001-10-10 2016-03-01 Ratiopharm Gmbh Remodelación y glicoconjugación de anticuerpos
CN1897812B (zh) * 2003-12-03 2011-08-17 生物种属学股份公司 糖聚乙二醇化的粒细胞集落刺激因子
KR20080021590A (ko) 2005-03-24 2008-03-07 네오스 테크놀로지스, 인크. 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현
US20060246544A1 (en) 2005-03-30 2006-11-02 Neose Technologies,Inc. Manufacturing process for the production of peptides grown in insect cell lines
WO2007056524A2 (en) 2005-11-09 2007-05-18 Neose Technologies, Inc. St3gal-1/st6galnac-1 chimeras
JP2010505874A (ja) * 2006-10-03 2010-02-25 ノヴォ ノルディスク アー／エス ポリペプチドコンジュゲートの精製方法
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
WO2005121332A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated st6galnaci polypeptides and nucleic acids

Also Published As

Publication number Publication date
WO2012016984A1 (en) 2012-02-09
MX2013001447A (es) 2013-11-20
CA2807397A1 (en) 2012-02-09
US20140057316A1 (en) 2014-02-27
EP2415779A1 (en) 2012-02-08
EP2415779B1 (en) 2015-01-21
CN103154017A (zh) 2013-06-12
PT2415779E (pt) 2015-05-13
ES2534936T3 (es) 2015-04-30
US9222081B2 (en) 2015-12-29
EP2601207A1 (en) 2013-06-12
JP5984267B2 (ja) 2016-09-06
EP2601207B1 (en) 2016-12-28
CA2807397C (en) 2019-03-19
JP2013535207A (ja) 2013-09-12

Similar Documents

Publication Publication Date Title
CN103154017B (zh) 生产和纯化活性可溶唾液酸转移酶的方法
JP7142021B2 (ja) 糖タンパク質製造プロセス
TW516962B (en) A human TNFR1-IgG1 preparation
US20220154155A1 (en) Manufacturing of glycoproteins
US20030040037A1 (en) In vitro modification of glycosylation patterns of recombinant glycopeptides
US20080206810A1 (en) Truncated St6galnaci Polypeptides and Nucleic Acids
JP2008538181A (ja) 昆虫細胞系において増殖させたペプチドを生産するための製造方法
US9670271B2 (en) Production of recombinant proteins with simple glycoforms
EP2240578B1 (en) Substantially animal protein-free recombinant furin and methods for producing same
CN102482674B (zh) 制备具有高唾液酸含量的重组糖蛋白的方法
JP2013516991A (ja) 治療用アピラ−ゼ構築物、アピラ−ゼ作用物質、及び製造方法
JP2018504121A (ja) 汚染物質たる宿主細胞タンパク質を含む材料からの組換えヒトα−ガラクトシダーゼＡの精製方法
EP0654529B1 (en) alpha-2,8-SIALYLTRANSFERASE
CN109312368B (zh) 用于产生衍生自真菌工程化宿主的复合聚糖的手段和方法
US20200277641A1 (en) Genetically engineered eukaryotic cells producing sialylated glycoproteins
KR20230088811A (ko) 알칼리성 포스파타제의 제조 동안 총 시알산 함량(tsac)을 제어하는 방법
WO2004092218A1 (ja) 組換えアンチトロンビンの製造方法
AU2007201550A1 (en) In vitro modification of glycosylation patterns of recombinant glycopeptides
HK1017903B (en) Process for controlling sialylation of proteins produced by mammalian cell culture

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: RATIOPHARM GMBH

Free format text: FORMER OWNER: BIOGENERIX AG

Effective date: 20140715

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20140715

Address after: Ulm

Applicant after: Ratiopharm GmbH

Address before: Ulm

Applicant before: Biogenerix AG

C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160203

Termination date: 20180802

CF01 Termination of patent right due to non-payment of annual fee