CA2790007C - Anticorps anti-her2 completement humanise, son procede de preparation et son utilisation - Google Patents
Anticorps anti-her2 completement humanise, son procede de preparation et son utilisation Download PDFInfo
- Publication number
- CA2790007C CA2790007C CA2790007A CA2790007A CA2790007C CA 2790007 C CA2790007 C CA 2790007C CA 2790007 A CA2790007 A CA 2790007A CA 2790007 A CA2790007 A CA 2790007A CA 2790007 C CA2790007 C CA 2790007C
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- her2
- fully human
- seq
- human anti
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title description 7
- 239000002773 nucleotide Substances 0.000 claims abstract description 20
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 20
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 16
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 16
- 239000013604 expression vector Substances 0.000 claims abstract description 8
- 238000011282 treatment Methods 0.000 claims abstract description 8
- 239000003814 drug Substances 0.000 claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 claims abstract 2
- 206010006187 Breast cancer Diseases 0.000 claims description 17
- 208000026310 Breast neoplasm Diseases 0.000 claims description 17
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 description 30
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 description 25
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 229960000575 trastuzumab Drugs 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 230000000259 anti-tumor effect Effects 0.000 description 4
- 230000006907 apoptotic process Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 238000012353 t test Methods 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000005917 in vivo anti-tumor Effects 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- 206010055113 Breast cancer metastatic Diseases 0.000 description 2
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108091007960 PI3Ks Proteins 0.000 description 2
- 108090000430 Phosphatidylinositol 3-kinases Proteins 0.000 description 2
- 102000003993 Phosphatidylinositol 3-kinases Human genes 0.000 description 2
- 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 229940022353 herceptin Drugs 0.000 description 2
- 210000005104 human peripheral blood lymphocyte Anatomy 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- XUHRVZXFBWDCFB-QRTDKPMLSA-N (3R)-4-[[(3S,6S,9S,12R,15S,18R,21R,24R,27R,28R)-12-(3-amino-3-oxopropyl)-6-[(2S)-butan-2-yl]-3-(2-carboxyethyl)-18-(hydroxymethyl)-28-methyl-9,15,21,24-tetrakis(2-methylpropyl)-2,5,8,11,14,17,20,23,26-nonaoxo-1-oxa-4,7,10,13,16,19,22,25-octazacyclooctacos-27-yl]amino]-3-[[(2R)-2-[[(3S)-3-hydroxydecanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoic acid Chemical compound CCCCCCC[C@H](O)CC(=O)N[C@H](CC(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H]1[C@@H](C)OC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CO)NC(=O)[C@@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC1=O)[C@@H](C)CC XUHRVZXFBWDCFB-QRTDKPMLSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- AOJJSUZBOXZQNB-VTZDEGQISA-N 4'-epidoxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-VTZDEGQISA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102000001301 EGF receptor Human genes 0.000 description 1
- 108060006698 EGF receptor Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- HTIJFSOGRVMCQR-UHFFFAOYSA-N Epirubicin Natural products COc1cccc2C(=O)c3c(O)c4CC(O)(CC(OC5CC(N)C(=O)C(C)O5)c4c(O)c3C(=O)c12)C(=O)CO HTIJFSOGRVMCQR-UHFFFAOYSA-N 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 101001003233 Homo sapiens Immediate early response gene 2 protein Proteins 0.000 description 1
- 102100020702 Immediate early response gene 2 protein Human genes 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108010011536 PTEN Phosphohydrolase Proteins 0.000 description 1
- 102000014160 PTEN Phosphohydrolase Human genes 0.000 description 1
- 229930012538 Paclitaxel Natural products 0.000 description 1
- 102000004503 Perforin Human genes 0.000 description 1
- 108010056995 Perforin Proteins 0.000 description 1
- KHGNFPUMBJSZSM-UHFFFAOYSA-N Perforine Natural products COC1=C2CCC(O)C(CCC(C)(C)O)(OC)C2=NC2=C1C=CO2 KHGNFPUMBJSZSM-UHFFFAOYSA-N 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 102100024952 Protein CBFA2T1 Human genes 0.000 description 1
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 description 1
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 102100024547 Tensin-1 Human genes 0.000 description 1
- 108010088950 Tensins Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 229940045799 anthracyclines and related substance Drugs 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000022534 cell killing Effects 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 238000009104 chemotherapy regimen Methods 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 239000012916 chromogenic reagent Substances 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000006552 constitutive activation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229960004679 doxorubicin Drugs 0.000 description 1
- 238000009261 endocrine therapy Methods 0.000 description 1
- 229940034984 endocrine therapy antineoplastic and immunomodulating agent Drugs 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 229960001904 epirubicin Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
- 102000051957 human ERBB2 Human genes 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- ZDZOTLJHXYCWBA-BSEPLHNVSA-N molport-006-823-826 Chemical compound O([C@H]1[C@H]2[C@@](C([C@H](O)C3=C(C)[C@@H](OC(=O)[C@H](O)[C@@H](NC(=O)OC(C)(C)C)C=4C=CC=CC=4)C[C@@]1(O)C3(C)C)=O)(C)[C@@H](O)C[C@H]1OC[C@]12OC(=O)C)C(=O)C1=CC=CC=C1 ZDZOTLJHXYCWBA-BSEPLHNVSA-N 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 229960001592 paclitaxel Drugs 0.000 description 1
- 229930192851 perforin Natural products 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 102000035160 transmembrane proteins Human genes 0.000 description 1
- 108091005703 transmembrane proteins Proteins 0.000 description 1
- 230000005747 tumor angiogenesis Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3015—Breast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Oncology (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pregnancy & Childbirth (AREA)
- Gynecology & Obstetrics (AREA)
- Engineering & Computer Science (AREA)
- Reproductive Health (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne un anticorps anti-HER2 complètement humanisé, ayant la séquence d'acides aminés de région variable de chaîne lourde telle que définie dans SEQ ID NO : 6 et la séquence d'acides aminés de région variable de chaîne légère telle que définie dans SEQ ID NO : 8. L'invention concerne également la séquence nucléotidique codant pour l'anticorps, le vecteur d'expression et la cellule hôte comprenant la séquence nucléotidique, et l'utilisation de l'anticorps pour la fabrication du médicament pour le traitement d'une tumeur.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010125241.0 | 2010-02-25 | ||
| CN201010125241.0A CN102167742B (zh) | 2010-02-25 | 2010-02-25 | 一种全人源抗her2单克隆抗体、其制备方法及用途 |
| PCT/CN2010/000511 WO2011103700A1 (fr) | 2010-02-25 | 2010-04-16 | Anticorps anti-her2 complètement humanisé, son procédé de préparation et son utilisation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2790007A1 CA2790007A1 (fr) | 2011-09-01 |
| CA2790007C true CA2790007C (fr) | 2017-02-14 |
Family
ID=44489082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2790007A Active CA2790007C (fr) | 2010-02-25 | 2010-04-16 | Anticorps anti-her2 completement humanise, son procede de preparation et son utilisation |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8974785B2 (fr) |
| EP (1) | EP2540745B1 (fr) |
| JP (1) | JP5690356B2 (fr) |
| CN (1) | CN102167742B (fr) |
| BR (1) | BR112012021327B8 (fr) |
| CA (1) | CA2790007C (fr) |
| WO (1) | WO2011103700A1 (fr) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2371860A1 (fr) | 2010-04-05 | 2011-10-05 | Fundació Privada Institut d'Investigació Oncològica de Vall d'Hebron | Anticorps reconnaissant le facteur d'inhibition de la leucémie humaine (LIF) et utilisation d'anticorps anti-LIF pour le traitement de maladies associées à la prolifération de cellules indésirables |
| CN102492039B (zh) * | 2011-11-21 | 2015-03-11 | 无锡天演生物技术有限公司 | 全人源抗人her2单抗 |
| CN102993305B (zh) * | 2012-11-16 | 2015-05-13 | 上海赛伦生物技术有限公司 | 人源抗人表皮生长因子受体抗体及其编码基因与应用 |
| KR101453462B1 (ko) | 2013-05-16 | 2014-10-23 | 앱클론(주) | Her2에 특이적으로 결합하는 항체 |
| US9738726B2 (en) | 2013-06-11 | 2017-08-22 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | HER2-specific monoclonal antibodies and conjugates thereof |
| CN104447993A (zh) * | 2013-09-13 | 2015-03-25 | 上海海思太科药业有限公司 | 一种高活性全人源抗her2单克隆抗体、其制备方法及用途 |
| HUE055269T2 (hu) * | 2013-11-19 | 2021-11-29 | Remegen Co Ltd | Anti-HER2 ellenanyag és konjugátum |
| KR101608301B1 (ko) | 2014-08-22 | 2016-04-12 | 앱클론(주) | Her2에 특이적으로 결합하는 항체 |
| TWI758784B (zh) * | 2014-09-12 | 2022-03-21 | 美商建南德克公司 | 抗her2抗體及免疫結合物 |
| CN104497140B (zh) * | 2014-11-26 | 2018-08-31 | 嘉和生物药业有限公司 | 一种全人源her2抗体、其编码基因及应用 |
| CN104530236B (zh) * | 2014-11-26 | 2018-02-23 | 嘉和生物药业有限公司 | 一种全人源her2抗体、其编码基因及应用 |
| CN104650228B (zh) * | 2014-11-26 | 2017-11-28 | 嘉和生物药业有限公司 | 一种全人源her2抗体、其编码基因及应用 |
| CN105985435B (zh) * | 2015-01-30 | 2019-10-15 | 嘉和生物药业有限公司 | 全人源her2抗体的突变抗体及其编码基因和应用 |
| MA43345A (fr) | 2015-10-02 | 2018-08-08 | Hoffmann La Roche | Conjugués anticorps-médicaments de pyrrolobenzodiazépine et méthodes d'utilisation |
| MA44334A (fr) | 2015-10-29 | 2018-09-05 | Novartis Ag | Conjugués d'anticorps comprenant un agoniste du récepteur de type toll |
| CN106831986B (zh) * | 2015-12-04 | 2020-01-21 | 浙江大学 | 一种抗人her2抗体及其编码基因和应用 |
| CN106831987B (zh) * | 2015-12-04 | 2020-01-21 | 浙江大学 | 一种抗人her2抗体及其编码基因和应用 |
| MY191926A (en) | 2016-12-01 | 2022-07-18 | Regeneron Pharma | Radiolabeled anti-pd-l1 antibodies for immuno-pet imaging |
| CN108610419B (zh) * | 2016-12-09 | 2020-08-18 | 泰州迈博太科药业有限公司 | 一种人源化抗her2单克隆抗体 |
| HRP20240195T8 (hr) | 2016-12-19 | 2024-05-24 | Medimmune Limited | Antitijela na lif i njihove primjene |
| US10583191B2 (en) | 2016-12-19 | 2020-03-10 | Mosaic Biomedicals Slu | Antibodies against LIF and uses thereof |
| AR111651A1 (es) | 2017-04-28 | 2019-08-07 | Novartis Ag | Conjugados de anticuerpos que comprenden agonistas del receptor de tipo toll y terapias de combinación |
| SG10201801219VA (en) * | 2018-02-13 | 2019-09-27 | Agency Science Tech & Res | Anti-HER2 Antibodies |
| CN113480657B (zh) * | 2021-08-06 | 2023-01-20 | 南京融捷康生物科技有限公司 | 针对her2的单域抗体及其衍生蛋白和应用 |
| EP4269432A1 (fr) | 2022-04-26 | 2023-11-01 | Universite de Rouen Normandie | Production d'anticorps thérapeutiques par le phaeodctylum tricornutum des micro-algues |
| WO2024030341A1 (fr) | 2022-07-30 | 2024-02-08 | Pinetree Therapeutics, Inc. | Compositions pour la dégradation lysosomale ciblée et leurs procédés d'utilisation |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2103059C (fr) * | 1991-06-14 | 2005-03-22 | Paul J. Carter | Methode de production d'anticorps humanises |
| DE60037896D1 (de) * | 1999-07-29 | 2008-03-13 | Medarex Inc | Menschliche antikörper gegen her2/neu |
| CN1210308C (zh) * | 2001-11-16 | 2005-07-13 | 上海中信国健药业有限公司 | 人源化抗her2单克隆抗体及其制法和药物组合物 |
| DK1501856T3 (da) * | 2002-04-10 | 2013-03-25 | Genentech Inc | Anti-HER2 antistofvarianter |
| WO2008019290A2 (fr) * | 2006-08-04 | 2008-02-14 | Astrazeneca Ab | Anticorps anti-erbb2 |
| CN101165068B (zh) * | 2006-10-18 | 2011-09-21 | 上海复旦张江生物医药股份有限公司 | 抗HER2/ErbB2抗原的单克隆抗体及其制备方法和药物组合物 |
| PL2247304T3 (pl) * | 2008-04-02 | 2017-01-31 | Macrogenics, Inc. | Przeciwciała specyficzne wobec HER2/neu oraz sposoby ich zastosowania |
-
2010
- 2010-02-25 CN CN201010125241.0A patent/CN102167742B/zh active Active
- 2010-04-16 EP EP10846324.1A patent/EP2540745B1/fr active Active
- 2010-04-16 US US13/579,180 patent/US8974785B2/en active Active
- 2010-04-16 JP JP2012554185A patent/JP5690356B2/ja active Active
- 2010-04-16 CA CA2790007A patent/CA2790007C/fr active Active
- 2010-04-16 BR BR112012021327A patent/BR112012021327B8/pt active IP Right Grant
- 2010-04-16 WO PCT/CN2010/000511 patent/WO2011103700A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012021327B1 (pt) | 2020-05-05 |
| WO2011103700A1 (fr) | 2011-09-01 |
| JP5690356B2 (ja) | 2015-03-25 |
| CN102167742A (zh) | 2011-08-31 |
| JP2013520180A (ja) | 2013-06-06 |
| EP2540745A4 (fr) | 2013-09-11 |
| BR112012021327A2 (pt) | 2017-06-20 |
| CA2790007A1 (fr) | 2011-09-01 |
| EP2540745A1 (fr) | 2013-01-02 |
| EP2540745B1 (fr) | 2015-09-30 |
| CN102167742B (zh) | 2014-05-14 |
| EP2540745A9 (fr) | 2013-02-27 |
| US8974785B2 (en) | 2015-03-10 |
| US20120309942A1 (en) | 2012-12-06 |
| BR112012021327B8 (pt) | 2021-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2790007C (fr) | Anticorps anti-her2 completement humanise, son procede de preparation et son utilisation | |
| JP6359372B2 (ja) | ＤＡＲＰｉｎを含む二重特異キメラ蛋白質 | |
| US11155632B2 (en) | Anti-CD47 monoclonal antibody and use thereof | |
| EP3954712A1 (fr) | Anticorps anti-pd-l1/vegf bifonctionnel et son utilisation | |
| WO2020043184A1 (fr) | Anticorps bifonctionnel anti-pd-1 et anti-vegfa, composition pharmaceutique et utilisation associées | |
| US8664368B2 (en) | Fully human monoclonal antibody to VEGF, preparation method and use thereof | |
| CA3196940A1 (fr) | Anticorps anti-her3 et conjugue anticorps anti-her3-medicament et leur utilisation medicale | |
| JP2023539501A (ja) | 抗ｖｅｇｆ－抗ｐｄ－ｌ１二重特異性抗体、その医薬組成物およびその使用 | |
| CN114685675B (zh) | 双特异性抗体及其在治疗癌症中的用途 | |
| CN114181310A (zh) | 抗tigit抗体、其药物组合物及用途 | |
| IL304095A (en) | Mesothelin binding molecule and its application | |
| MX2011000117A (es) | Composicion farmaceutica para tratamiento y prevencion de cancer. | |
| WO2021244553A1 (fr) | Anticorps bispécifique tétravalent contre pd-1 et egfr | |
| CN113150156B (zh) | 抗tigit抗体及其用途 | |
| WO2021244554A1 (fr) | Anticorps bispécifique anti-pdl1 × egfr | |
| WO2023241656A1 (fr) | Anticorps bispécifique contenant un anticorps anti-cldn18.2, composition pharmaceutique et utilisation associées | |
| US10611833B2 (en) | Humanized anti-human epidermal growth factor receptor antibody and application thereof | |
| CN113336848B (zh) | 一种抗pd-1抗体及其用途 | |
| CN113336847B (zh) | 一种抗pd-1抗体 | |
| WO2016163433A1 (fr) | Composition comprenant un anticorps anti-fgfr2 et un autre agent | |
| CN113956359B (zh) | 一种抗体及其在抗肿瘤中的应用 | |
| CN114539415B (zh) | 一种抗PD-L1/VEGF/TGF-β多特异性抗体及其用途 | |
| US20220089727A1 (en) | Anti-CD47 monoclonal antibody and use thereof | |
| CN106606784B (zh) | 一种靶向表达egfr肿瘤细胞的前抗体偶联药物及其应用 | |
| CN115698087A (zh) | 抗pdl1×kdr的双特异性抗体 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20141205 |