CA2763955C - Production biotechnologique de chondroitine - Google Patents
Production biotechnologique de chondroitine Download PDFInfo
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- CA2763955C CA2763955C CA2763955A CA2763955A CA2763955C CA 2763955 C CA2763955 C CA 2763955C CA 2763955 A CA2763955 A CA 2763955A CA 2763955 A CA2763955 A CA 2763955A CA 2763955 C CA2763955 C CA 2763955C
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- Prior art keywords
- chondroitin
- fermentation
- process according
- strain
- polysaccharide
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- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000013060 ultrafiltration and diafiltration Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Steroid Compounds (AREA)
Abstract
L'invention porte sur un procédé innovant pour la production de chondroïtine à concentration élevée, par fermentation de bactéries génétiquement mutées.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI2009A000923 | 2009-05-25 | ||
| ITMI2009A000923A IT1394311B1 (it) | 2009-05-25 | 2009-05-25 | Produzione biotecnologica di condroitina |
| PCT/EP2010/057129 WO2010136435A1 (fr) | 2009-05-25 | 2010-05-25 | Production biotechnologique de chondroïtine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2763955A1 CA2763955A1 (fr) | 2010-12-02 |
| CA2763955C true CA2763955C (fr) | 2018-11-27 |
Family
ID=41665067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2763955A Active CA2763955C (fr) | 2009-05-25 | 2010-05-25 | Production biotechnologique de chondroitine |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8592186B2 (fr) |
| EP (1) | EP2435578B1 (fr) |
| CN (1) | CN102459625B (fr) |
| CA (1) | CA2763955C (fr) |
| DK (1) | DK2435578T3 (fr) |
| ES (1) | ES2676755T3 (fr) |
| HU (1) | HUE038895T2 (fr) |
| IT (1) | IT1394311B1 (fr) |
| PL (1) | PL2435578T3 (fr) |
| PT (1) | PT2435578T (fr) |
| SI (1) | SI2435578T1 (fr) |
| WO (1) | WO2010136435A1 (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITMI20110505A1 (it) | 2011-03-29 | 2012-09-30 | Altergon Sa | Processo per la preparazione di condroitine solfatate in posizione 3 sull'acido glucuronico |
| HUE030096T2 (en) * | 2011-05-20 | 2017-04-28 | Gnosis Spa | Shark-like chondroitin sulfate and process |
| US8664196B2 (en) | 2011-05-20 | 2014-03-04 | Gnosis S.P.A. | Shark-like chondroitin sulphate and process for the preparation thereof |
| CN102618597A (zh) * | 2012-04-06 | 2012-08-01 | 江南大学 | 大肠杆菌工程菌及其生产软骨素的方法 |
| ITMI20120896A1 (it) | 2012-05-23 | 2013-11-24 | Bongulielmi Reto | Condroitina per uso in medicina |
| GB201211256D0 (en) | 2012-06-25 | 2012-08-08 | Glaxosmithkline Biolog Sa | Fermentation process |
| ITMI20121316A1 (it) | 2012-07-27 | 2014-01-28 | Altergon Sa | Complessi di condroitina ad assorbimento transcutaneo |
| CN102965414B (zh) * | 2012-11-27 | 2014-10-22 | 江南大学 | 一种发酵液中提取硫酸软骨素的方法 |
| CN103352061A (zh) * | 2013-07-23 | 2013-10-16 | 江南大学 | 一种提高发酵法生产硫酸软骨素类似物产量的方法 |
| JP6569530B2 (ja) * | 2013-10-02 | 2019-09-04 | 味の素株式会社 | ヘパロサン生産細菌及びヘパロサンの製造法 |
| WO2015066893A1 (fr) * | 2013-11-08 | 2015-05-14 | 青岛贝尔特生物科技有限公司 | Procédé de préparation de sulfate de chondroïtine de faible masse moléculaire pour le traitement d'une myocardite |
| FR3016637B1 (fr) * | 2014-01-23 | 2020-11-27 | Centre Nat Rech Scient | Procede de production in vivo de glycosaminoglycane |
| WO2017184085A1 (fr) * | 2016-04-21 | 2017-10-26 | Agency For Science, Technology And Research | Particules de type noyau-enveloppe |
| CN113603732B (zh) * | 2021-08-25 | 2024-02-13 | 江南大学 | 一种非动物源硫酸软骨素寡糖及其制备方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7892798B2 (en) * | 1999-06-25 | 2011-02-22 | Evonik Degussa Gmbh | Nucleic acid molecules encoding metabolic regulatory proteins from Corynebacterium glutamicum, useful for increasing the production of methionone by a microorganism |
| ITMI991465A1 (it) * | 1999-07-02 | 2001-01-02 | Inalco Spa | Processo per la preparazione dei polisaccaridi k4 e k5 da escherichiacoli |
| JP2004512013A (ja) * | 2000-04-25 | 2004-04-22 | デ アンジェリス，ポール，エル． | コンドロイチンシンターゼ遺伝子並びにその生成方法及び使用方法 |
| ITMI20012200A1 (it) * | 2001-10-22 | 2003-04-22 | Ibsa Inst Biochimique Sa | Processo per la preparazione di condroitin solfati dal polisaccaride k4 e prodotti ottenuti |
| BRPI0515210A (pt) * | 2004-08-19 | 2008-07-08 | Nature Technology Corp | método para a produção de fermentação de alimentação em batelada de dna de plasmìdio super-enrolado fechado covalentemente e método para a produção de fermentação de dna de plasmìdio super-enrolado fechado covalentemente |
| US8283145B2 (en) * | 2007-04-24 | 2012-10-09 | Seikagaku Corporation | Chondroitin-producing bacterium and method of producing chondroitin |
| AR070568A1 (es) * | 2008-02-05 | 2010-04-21 | Aeterna Zentaris Gmbh | Bacterias recombinantes con un sistema de secrecion de hemolisina de e. coli y expresion y /o secrecion incrementada de hlya, proceso para producirlas, y sus usos |
-
2009
- 2009-05-25 IT ITMI2009A000923A patent/IT1394311B1/it active
-
2010
- 2010-05-25 PL PL10730105T patent/PL2435578T3/pl unknown
- 2010-05-25 US US13/322,185 patent/US8592186B2/en active Active
- 2010-05-25 CN CN201080032889.1A patent/CN102459625B/zh active Active
- 2010-05-25 EP EP10730105.3A patent/EP2435578B1/fr active Active
- 2010-05-25 PT PT107301053T patent/PT2435578T/pt unknown
- 2010-05-25 WO PCT/EP2010/057129 patent/WO2010136435A1/fr active Application Filing
- 2010-05-25 CA CA2763955A patent/CA2763955C/fr active Active
- 2010-05-25 ES ES10730105.3T patent/ES2676755T3/es active Active
- 2010-05-25 DK DK10730105.3T patent/DK2435578T3/en active
- 2010-05-25 HU HUE10730105A patent/HUE038895T2/hu unknown
- 2010-05-25 SI SI201031706T patent/SI2435578T1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2676755T3 (es) | 2018-07-24 |
| CN102459625B (zh) | 2015-02-11 |
| PL2435578T3 (pl) | 2018-10-31 |
| US20120135470A1 (en) | 2012-05-31 |
| SI2435578T1 (en) | 2018-07-31 |
| EP2435578A1 (fr) | 2012-04-04 |
| ITMI20090923A1 (it) | 2010-11-26 |
| DK2435578T3 (en) | 2018-07-30 |
| IT1394311B1 (it) | 2012-06-06 |
| PT2435578T (pt) | 2018-07-16 |
| HUE038895T2 (hu) | 2018-12-28 |
| CA2763955A1 (fr) | 2010-12-02 |
| WO2010136435A1 (fr) | 2010-12-02 |
| US8592186B2 (en) | 2013-11-26 |
| CN102459625A (zh) | 2012-05-16 |
| EP2435578B1 (fr) | 2018-05-23 |
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