CA2754826A1 - Live attenuated influenza virus vaccines comprising microrna response elements - Google Patents
Live attenuated influenza virus vaccines comprising microrna response elements Download PDFInfo
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- CA2754826A1 CA2754826A1 CA2754826A CA2754826A CA2754826A1 CA 2754826 A1 CA2754826 A1 CA 2754826A1 CA 2754826 A CA2754826 A CA 2754826A CA 2754826 A CA2754826 A CA 2754826A CA 2754826 A1 CA2754826 A1 CA 2754826A1
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| US15823309P | 2009-03-06 | 2009-03-06 | |
| US61/158,233 | 2009-03-06 | ||
| PCT/US2010/000709 WO2010101663A2 (en) | 2009-03-06 | 2010-03-08 | Live attenuated influenza virus vaccines comprising microrna response elements |
Publications (1)
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| CA2754826A1 true CA2754826A1 (en) | 2010-09-10 |
Family
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Family Applications (1)
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|---|---|---|---|
| CA2754826A Abandoned CA2754826A1 (en) | 2009-03-06 | 2010-03-08 | Live attenuated influenza virus vaccines comprising microrna response elements |
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| EP (1) | EP2403527A4 (OSRAM) |
| JP (1) | JP2012519484A (OSRAM) |
| CA (1) | CA2754826A1 (OSRAM) |
| WO (1) | WO2010101663A2 (OSRAM) |
Families Citing this family (38)
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| ES2351976T3 (es) | 2003-04-29 | 2011-02-14 | Avi Biopharma, Inc. | Composiciones para mejorar el transporte y la eficacia antisentido de análogos de ácidos nucleicos en células. |
| US8067571B2 (en) | 2005-07-13 | 2011-11-29 | Avi Biopharma, Inc. | Antibacterial antisense oligonucleotide and method |
| US8877206B2 (en) | 2007-03-22 | 2014-11-04 | Pds Biotechnology Corporation | Stimulation of an immune response by cationic lipids |
| US20100016215A1 (en) | 2007-06-29 | 2010-01-21 | Avi Biopharma, Inc. | Compound and method for treating myotonic dystrophy |
| US9789129B2 (en) | 2008-04-17 | 2017-10-17 | Pds Biotechnology Corporation | Stimulation of an immune response by enantiomers of cationic lipids |
| CA2754826A1 (en) * | 2009-03-06 | 2010-09-10 | Mount Sinai School Of Medicine | Live attenuated influenza virus vaccines comprising microrna response elements |
| US20110269665A1 (en) | 2009-06-26 | 2011-11-03 | Avi Biopharma, Inc. | Compound and method for treating myotonic dystrophy |
| WO2011131193A1 (en) * | 2010-04-24 | 2011-10-27 | Statens Serum Institut | Diagnosing and treating fibrotic diseases using micro-rna 17 |
| PL3333265T3 (pl) | 2010-05-14 | 2020-08-10 | Oregon Health & Science University | Rekombinowane wektory HCMV i RHCMV kodujące heterologiczny antygen wyizolowany z wirusa zapalenia wątroby typu B oraz ich zastosowania |
| WO2012051491A1 (en) * | 2010-10-14 | 2012-04-19 | The United States Of America, As Represented By The Secretary National Institutes Of Health | Compositions and methods for controlling neurotropic viral pathogenesis by micro-rna targeting |
| CN103648491B (zh) * | 2011-04-08 | 2017-07-14 | 法国国家健康医学研究院 | 用于抑制流感病毒复制的方法和药物组合物 |
| US9161948B2 (en) | 2011-05-05 | 2015-10-20 | Sarepta Therapeutics, Inc. | Peptide oligonucleotide conjugates |
| RS57397B8 (sr) | 2011-06-10 | 2020-01-31 | Univ Oregon Health & Science | Cmv glikoproteini i rekombinantni vektori |
| WO2013059498A1 (en) * | 2011-10-18 | 2013-04-25 | Geovax, Inc. | Mva vectors expressing polypeptides and having high level production in certain cell lines |
| EP3536789A1 (en) * | 2012-06-06 | 2019-09-11 | Boehringer Ingelheim International GmbH | Cell engineering using rnas |
| CN105163753B (zh) * | 2012-06-15 | 2018-12-21 | Pds生物科技公司 | 阳离子脂质疫苗组合物和使用方法 |
| US20150250872A1 (en) | 2012-09-21 | 2015-09-10 | Frank Bedu-Addo | Vaccine compositions and methods of use |
| ES2696703T3 (es) | 2013-03-05 | 2019-01-17 | Univ Oregon Health & Science | Vectores de citomegalovirus que permiten el control del direccionamiento de los linfocitos T |
| US10226528B2 (en) | 2013-03-15 | 2019-03-12 | The Trustees Of The University Of Pennsylvania | Vaccines with biomolecular adjuvants |
| CN104694541A (zh) | 2013-12-09 | 2015-06-10 | 江苏命码生物科技有限公司 | 禽流感病毒miRNA及其鉴定、检测和应用 |
| WO2015195881A1 (en) * | 2014-06-18 | 2015-12-23 | The Regents Of The University Of California | Methods and compositions for inhibiting infection by influenza and viruses |
| EP4194558A1 (en) | 2014-07-16 | 2023-06-14 | Oregon Health & Science University | Human cytomegalovirus comprising exogenous antigens |
| WO2016061200A1 (en) * | 2014-10-14 | 2016-04-21 | Texas Tech University System | Live attenuated viral vaccine created by self-attenuation with species-specific artificial microrna |
| WO2016088380A1 (ja) * | 2014-12-03 | 2016-06-09 | 公益財団法人ヒューマンサイエンス振興財団 | ムンプスウイルスの弱毒化方法、ムンプスウイルス、及び、生ワクチン |
| JP6816031B2 (ja) | 2015-02-10 | 2021-01-20 | オレゴン ヘルス アンド サイエンス ユニバーシティ | 非標準的なｃｄ８＋ｔ細胞応答を生成するのに有用な方法および組成物 |
| US11020417B2 (en) | 2015-06-04 | 2021-06-01 | Sarepta Therapeutics, Inc | Methods and compounds for treatment of lymphocyte-related diseases and conditions |
| US10927151B2 (en) | 2015-06-09 | 2021-02-23 | Sanofi Pasteur Inc. | Methods of optimizing nucleotide sequences encoding engineered influenza proteins |
| US10086063B2 (en) | 2015-09-23 | 2018-10-02 | Regents Of The University Of Minnesota | Methods of making and using live attenuated viruses |
| US10383935B2 (en) | 2015-09-23 | 2019-08-20 | Regents Of The University Of Minnesota | Methods of making and using live attenuated viruses |
| US11612652B2 (en) | 2015-11-13 | 2023-03-28 | Pds Biotechnology Corporation | Lipids as synthetic vectors to enhance antigen processing and presentation ex-vivo in dendritic cell therapy |
| AU2016355468B2 (en) * | 2015-11-20 | 2022-12-15 | Oregon Health & Science University | CMV vectors comprising microRNA recognition elements |
| US10590174B2 (en) * | 2016-07-08 | 2020-03-17 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Genomic sequences encoding for an attenuated mutant Zika virus |
| MA46588A (fr) | 2016-10-18 | 2021-04-14 | Univ Oregon Health & Science | Vecteurs de cytomégalovirus déclenchant des lymphocytes t limités par des molécules e de complexe majeur d'histocompatibilité |
| CN111511800B (zh) | 2017-10-30 | 2023-11-28 | 武田药品工业株式会社 | 灭活脂包膜病毒的环境相容性去污剂 |
| CN111511397A (zh) | 2017-12-22 | 2020-08-07 | 科达金尼克斯有限公司 | 具有密码子对去优化区域的重组病毒及其用于治疗癌症的用途 |
| CN112040977A (zh) | 2018-03-08 | 2020-12-04 | 科达金尼克斯有限公司 | 减毒黄病毒 |
| WO2019204571A1 (en) | 2018-04-20 | 2019-10-24 | Texas Tech University System | Self-attenuated prophylactic and therapeutic vaccines against pathogens |
| CN114072417B (zh) * | 2019-06-04 | 2024-05-24 | 凌斌 | 重组非结构蛋白1、含此的重组流感病毒及免疫组成物及其用于制备流感病毒疫苗组成物的用途 |
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| CN102304570B (zh) | 2002-11-13 | 2015-01-21 | 托马斯杰斐逊大学 | 用于癌症诊断和治疗的组合物和方法 |
| CA2522081C (en) * | 2003-04-23 | 2013-01-29 | Wisconsin Alumni Research Foundation | Recombinant influenza viruses holding a mutation in a transmembrane protein gene |
| US8145436B2 (en) * | 2003-07-15 | 2012-03-27 | The Trustees Of The University Of Pennsylvania | Method and systems for identifying micro-RNA targets and synthesizing novel micro-RNAs and uses of the same |
| US8106180B2 (en) * | 2003-08-07 | 2012-01-31 | Whitehead Institute For Biomedical Research | Methods and products for expression of micro RNAs |
| US7279275B2 (en) | 2003-11-03 | 2007-10-09 | Washington University | Methods and compositions for detection of segmented negative strand RNA viruses |
| CA2573007A1 (en) | 2004-07-02 | 2006-03-30 | Henry L. Niman | Copy choice recombination and uses thereof |
| US20070213293A1 (en) | 2005-04-08 | 2007-09-13 | Nastech Pharmaceutical Company Inc. | Rnai therapeutic for respiratory virus infection |
| US20070054872A1 (en) * | 2005-08-24 | 2007-03-08 | Mirus Bio Corporation | Regulatable or conditional expression systems |
| US20080045472A1 (en) * | 2006-03-31 | 2008-02-21 | Council Of Scientific And Industrial Research Bharat Biotech | Targets for human micro rnas in avian influenza virus (h5n1) genome |
| US9072701B2 (en) | 2006-04-21 | 2015-07-07 | St. Jude Children's Research Hospital | Avian influenza viruses, vaccines, compositions, formulations, and methods |
| WO2008140622A2 (en) * | 2006-12-22 | 2008-11-20 | The Penn State Research Foundation | Modified polymerases and attenuated viruses and methods of use thereof |
| CN101646769A (zh) * | 2007-02-20 | 2010-02-10 | 孟山都技术公司 | 无脊椎动物微rna |
| EP2139515B2 (en) * | 2007-03-30 | 2023-12-20 | The Research Foundation of the State University of New York | Attenuated viruses useful for vaccines |
| US20120115131A1 (en) * | 2007-05-31 | 2012-05-10 | Yale University | Genetic lesion associated with cancer |
| WO2009079592A2 (en) * | 2007-12-17 | 2009-06-25 | California Institute Of Technology | Modulating immune system development and function through microrna mir-146 |
| WO2009111892A1 (en) * | 2008-03-14 | 2009-09-17 | Ottawa Health Research Institute | Microrna mediated oncolytic targeting |
| CA2754826A1 (en) * | 2009-03-06 | 2010-09-10 | Mount Sinai School Of Medicine | Live attenuated influenza virus vaccines comprising microrna response elements |
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- 2010-03-08 EP EP10749069A patent/EP2403527A4/en not_active Withdrawn
- 2010-03-08 JP JP2011552950A patent/JP2012519484A/ja active Pending
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| US8883995B2 (en) | 2014-11-11 |
| EP2403527A4 (en) | 2012-11-14 |
| WO2010101663A3 (en) | 2010-10-28 |
| WO2010101663A2 (en) | 2010-09-10 |
| US20120148622A1 (en) | 2012-06-14 |
| JP2012519484A (ja) | 2012-08-30 |
| EP2403527A2 (en) | 2012-01-11 |
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