CA2753208C - Methods and compositions for the differentiation of stem cells - Google Patents

Methods and compositions for the differentiation of stem cells Download PDF

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Publication number
CA2753208C
CA2753208C CA2753208A CA2753208A CA2753208C CA 2753208 C CA2753208 C CA 2753208C CA 2753208 A CA2753208 A CA 2753208A CA 2753208 A CA2753208 A CA 2753208A CA 2753208 C CA2753208 C CA 2753208C
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cells
medium
differentiation
cell
pluripotent stem
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French (fr)
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CA2753208A1 (en
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Christine A. Daigh
Peter Fuhrken
Giorgia Salvagiotto
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Fujifilm Cellular Dynamics Inc
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Fujifilm Cellular Dynamics Inc
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/069Vascular Endothelial cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/165Vascular endothelial growth factor [VEGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2753208A 2009-02-20 2010-02-22 Methods and compositions for the differentiation of stem cells Active CA2753208C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15421009P 2009-02-20 2009-02-20
US61/154,210 2009-02-20
PCT/US2010/024881 WO2010096746A1 (en) 2009-02-20 2010-02-22 Methods and compositions for the differentiation of stem cells

Publications (2)

Publication Number Publication Date
CA2753208A1 CA2753208A1 (en) 2010-08-26
CA2753208C true CA2753208C (en) 2018-08-21

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CA2753208A Active CA2753208C (en) 2009-02-20 2010-02-22 Methods and compositions for the differentiation of stem cells

Country Status (5)

Country Link
US (1) US8557580B2 (enrdf_load_stackoverflow)
EP (1) EP2398897B1 (enrdf_load_stackoverflow)
JP (3) JP6189581B2 (enrdf_load_stackoverflow)
CA (1) CA2753208C (enrdf_load_stackoverflow)
WO (1) WO2010096746A1 (enrdf_load_stackoverflow)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230065354A (ko) 2007-09-28 2023-05-11 셀룰래리티 인코포레이티드 인간 태반 관류액 및 인간 태반-유래 중간체 천연 킬러 세포를 사용한 종양 억제 방법
CA2758003A1 (en) * 2009-04-13 2010-10-21 The Regents Of The University Of California Methods and compositions for stem cell cultures
EP2539437B1 (en) 2010-02-25 2017-08-02 The Johns Hopkins University SMOOTH MUSCLE-LIKE CELLS (SMLCs) DERIVED FROM HUMAN PLURIPOTENT STEM CELLS
US20130071927A1 (en) * 2010-05-05 2013-03-21 Sydney Ivf Limited Media and methods for cell culture
US20120064040A1 (en) * 2010-06-03 2012-03-15 The Regents Of The University Of California Serum free culture medium and supplement
NZ605505A (en) 2010-07-13 2015-02-27 Anthrogenesis Corp Methods of generating natural killer cells
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
CN102985531B (zh) * 2010-09-10 2019-04-23 国立大学法人东京大学 涉及由多能干细胞向血细胞分化的培养方法
CA2814440A1 (en) 2010-11-09 2012-05-18 Lonza Cologne Gmbh Method for controlling binding of cells to a substrate
CN110016463A (zh) 2010-11-15 2019-07-16 艾克塞利瑞提德生物技术公司 由人类滋养层干细胞生成神经干细胞
WO2012112776A2 (en) * 2011-02-16 2012-08-23 Salk Institute For Biological Studies Robust and efficient differentiation of human pluripotent stem cells to multipotent vascular progenitors
US9499789B2 (en) 2011-02-23 2016-11-22 Kyoto University Method for producing dendritic cells from pluripotent stem cells
WO2012141571A1 (en) * 2011-04-11 2012-10-18 M Abdullah Jaffar Ali Bin Protein-free culture media products
ES2653249T3 (es) * 2011-09-29 2018-02-06 Cellsnap, Llc Composiciones y métodos para ensayos de toxigenicidad
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
CA2857295C (en) * 2011-12-01 2021-06-29 New York Stem Cell Foundation Automated system for producing induced pluripotent stem cells or differentiated cells
US9428732B2 (en) 2011-12-05 2016-08-30 Primorigen Biosciences, Inc. Compositions and methods for differentiating pluripotent stem cells into primitive blood cells and uses thereof
WO2013130820A1 (en) * 2012-02-29 2013-09-06 The Johns Hopkins University Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
EP2922866B1 (en) 2012-11-21 2019-01-23 Merz Pharma GmbH & Co. KGaA Means and methods for determination of botulinum neurotoxin biological activity
EP2925860B8 (en) 2012-11-30 2019-12-25 Accelerated BioSciences Corp. Methods of differentiating stem cells by modulating mir-124
WO2014100779A1 (en) * 2012-12-21 2014-06-26 Advanced Cell Technology, Inc. Methods ofr production of platelets from pluripotent stem cells and compositions thereof
EP2953635A4 (en) 2013-02-05 2016-10-26 Anthrogenesis Corp NATURAL KILLER CELLS FROM PLAZENTA
US9994825B2 (en) 2013-03-15 2018-06-12 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
EP2777703B1 (en) * 2013-03-15 2016-09-14 Lonza Cologne GmbH Compositions and methods for enhancing the therapeutic potential of stem cells
US9506037B2 (en) 2013-03-15 2016-11-29 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
US11976110B2 (en) 2013-06-28 2024-05-07 Merz Pharma Gmbh & Co. Kgaa Means and methods for the determination of the biological activity of neurotoxin polypeptides in cells
US10959997B2 (en) 2013-12-27 2021-03-30 Kyoto Prefectural Public University Corporation Combined agent for cell therapy of corneal endothelial cell
WO2015119642A1 (en) * 2014-02-10 2015-08-13 The Johns Hopkins University Low oxygen tension enhances endothelial fate of human pluripotent stem cells
JP6549599B2 (ja) 2014-02-19 2019-07-24 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー in vitro試験系におけるボツリヌス神経毒に対する細胞の感度を標準化し且つ増加させるためのガングリオシド
AU2015249371B2 (en) 2014-04-24 2020-04-30 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
AU2015353521B2 (en) 2014-11-26 2021-11-04 Accelerated Biosciences Corp. Induced hepatocytes and uses thereof
AU2015367329B2 (en) 2014-12-19 2021-02-18 Merz Pharma Gmbh & Co. Kgaa Means and methods for the determination of the biological activity of BoNT/E in cells
US10626372B1 (en) 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
TWI704156B (zh) 2015-03-04 2020-09-11 德商曼茲法瑪股份有限公司 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法
WO2017078807A1 (en) * 2015-11-04 2017-05-11 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
CN108368520B (zh) 2015-11-04 2023-01-17 菲特治疗公司 多能细胞的基因组工程改造
CN111334463B (zh) 2016-05-18 2024-02-20 学校法人庆应义塾 类器官培养用细胞培养基、培养方法及类器官
JP2021509812A (ja) 2018-01-05 2021-04-08 プレートレット バイオジェネシス, インコーポレイテッド 巨核球を産生するための組成物および方法
EP3813853A4 (en) 2018-06-29 2022-04-06 Platelet Biogenesis, Inc. DELIVERY COMPOSITIONS AND METHODS OF USE
EP3847243A1 (en) * 2018-09-07 2021-07-14 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN110438065B (zh) * 2019-07-25 2020-06-05 中山大学 一种诱导人诱导性多能干细胞分化为内皮祖细胞的方法
US20220411755A1 (en) * 2019-11-14 2022-12-29 Amgen Inc. Hematopoietic precursor cell production
EP4206320A4 (en) * 2020-08-21 2025-02-12 JSR Corporation METHOD FOR CULTIVATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS, CULTURE OF HUMAN INDUCED PLURIPOTENT STEM CELLS AND METHOD FOR THE PRODUCTION OF CEREBRAL ORGANOIDS
WO2024018018A1 (fr) 2022-07-21 2024-01-25 Etablissement Francais Du Sang Utilisation d'un composé vitisine pour la production de cellules hématopoïétiques
WO2025126208A1 (en) * 2023-12-12 2025-06-19 Accellta Ltd. Media for maintaining, differentiating, or both, pluripotent stem cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018737B1 (en) 1979-04-11 1983-11-09 Mitsui Petrochemical Industries, Ltd. Process for producing spherical carrier particles for olefin polymerisation catalyst, catalyst comprising such carrier particles, and use of such catalyst in olefin polymerisation
GB9807935D0 (en) * 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ,インコーポレイテッド 胚性幹細胞血清置換
CN1187090C (zh) 1999-03-25 2005-02-02 三菱制药株式会社 间质性肺炎、肺纤维化症的预防和治疗药
AU5250400A (en) 1999-06-18 2001-01-09 Mitsubishi Pharma Corporation Osteogenesis promoters
JP4563573B2 (ja) * 2000-12-07 2010-10-13 株式会社カネカ 抗原およびこの抗原を識別するモノクローナル抗体
MXPA04007196A (es) 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
US6664266B2 (en) 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
CA2482598C (en) 2002-04-22 2012-09-25 The Board Of Trustees Of The Leland Stanford Junior University Peptide inhibitors of protein kinase c
WO2004029200A2 (en) 2002-09-26 2004-04-08 Children's Medical Center Corporation Method of enhancing proliferation and/or hematopoietic differentiation of stem cells
AU2005282414C1 (en) * 2004-09-08 2011-04-07 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
GB2432846B (en) 2004-09-08 2009-12-30 Wisconsin Alumni Res Found Medium and culture of embryonic stem cells
AU2005302258B2 (en) 2004-11-01 2010-10-21 Wisconsin Alumni Research Foundation Platelets from stem cells
US7732445B2 (en) 2006-07-21 2010-06-08 Northwestern University Myosin light chain kinase inhibitor compounds, compostions and related methods of use
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
JP5991796B2 (ja) 2007-06-29 2016-09-14 セルラー ダイナミクス インターナショナル, インコーポレイテッド 胚性幹細胞培養のための自動化された方法および装置
US8513012B2 (en) * 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells

Also Published As

Publication number Publication date
JP2012518415A (ja) 2012-08-16
JP2017148079A (ja) 2017-08-31
EP2398897A1 (en) 2011-12-28
JP6189581B2 (ja) 2017-08-30
US8557580B2 (en) 2013-10-15
EP2398897B1 (en) 2017-06-28
CA2753208A1 (en) 2010-08-26
WO2010096746A1 (en) 2010-08-26
JP2016019543A (ja) 2016-02-04
US20100216181A1 (en) 2010-08-26
JP6691509B2 (ja) 2020-04-28

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