CA2735690A1 - Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases - Google Patents
Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases Download PDFInfo
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- CA2735690A1 CA2735690A1 CA2735690A CA2735690A CA2735690A1 CA 2735690 A1 CA2735690 A1 CA 2735690A1 CA 2735690 A CA2735690 A CA 2735690A CA 2735690 A CA2735690 A CA 2735690A CA 2735690 A1 CA2735690 A1 CA 2735690A1
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01009—Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
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- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Theoretical Computer Science (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10079208P | 2008-09-29 | 2008-09-29 | |
| US61/100,792 | 2008-09-29 | ||
| PCT/US2009/058827 WO2010037112A1 (en) | 2008-09-29 | 2009-09-29 | IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2735690A1 true CA2735690A1 (en) | 2010-04-01 |
Family
ID=41402186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2735690A Abandoned CA2735690A1 (en) | 2008-09-29 | 2009-09-29 | Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases |
Country Status (13)
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|---|---|
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| EP (2) | EP2821484B1 (https=) |
| JP (1) | JP5846912B2 (https=) |
| KR (1) | KR101659101B1 (https=) |
| CN (1) | CN102186973B (https=) |
| AU (1) | AU2009296225B2 (https=) |
| BR (1) | BRPI0913682A2 (https=) |
| CA (1) | CA2735690A1 (https=) |
| ES (1) | ES2521675T3 (https=) |
| MX (1) | MX2011003238A (https=) |
| NZ (1) | NZ591244A (https=) |
| WO (1) | WO2010037112A1 (https=) |
| ZA (2) | ZA201101367B (https=) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
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| US20160222370A1 (en) * | 2008-09-29 | 2016-08-04 | Butamax Advanced Biofuels Llc | Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof |
| CA2736420A1 (en) * | 2008-09-29 | 2010-04-01 | Butamaxtm Advanced Biofuels Llc | Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria |
| US9206447B2 (en) | 2008-09-29 | 2015-12-08 | Butamax Advanced Biofuels Llc | Recombinant yeast host cells comprising FE-S cluster proteins |
| AU2009296227A1 (en) * | 2008-09-29 | 2010-04-01 | Butamax(Tm) Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
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| US8465964B2 (en) | 2008-11-13 | 2013-06-18 | Butamax (TM) Advanced Biofules LLC | Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis |
| US8614085B2 (en) * | 2009-02-27 | 2013-12-24 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving a multidrug efflux pump gene |
| BRPI1006492A2 (pt) | 2009-04-13 | 2019-09-24 | Butamax Advanced Biofuels Llc | método para a recuperação de butanol a partir de um meio de fermentação, método para a produção de butanol e mistura de duas fases |
| BR112012003241A2 (pt) * | 2009-08-12 | 2015-09-01 | Gevo Inc | Localização de via de isobutanol citosólico para a produção de isobutanol |
| EP2483401B1 (en) | 2009-09-29 | 2017-06-21 | Butamax (TM) Advanced Biofuels LLC | Improved yeast production host cells |
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| US20110195505A1 (en) * | 2009-10-08 | 2011-08-11 | Butamax(Tm) Advanced Biofuels Llc | Bacterial strains for butanol production |
| BR112012012211A2 (pt) | 2009-11-23 | 2017-05-09 | Butamax (Tm) Advanced Biofuels Llc | método para a recuperação de butanol e método para a produção de butanol |
| CA2779884A1 (en) | 2009-11-23 | 2011-05-26 | Butamax(Tm) Advanced Biofuels Llc | Method for producing butanol using extractive fermentation with electrolyte addition |
| WO2011066356A1 (en) * | 2009-11-24 | 2011-06-03 | Gevo, Inc. | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| NZ600509A (en) | 2009-12-29 | 2014-08-29 | Butamax Tm Advanced Biofuels | Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols |
| NZ600585A (en) | 2009-12-29 | 2014-07-25 | Butamax Tm Advanced Biofuels | Expression of hexose kinase in recombinant host cells |
| PH12012501658A1 (en) | 2010-02-17 | 2023-04-28 | Butamax Tm Advanced Biofuels | Improving activity of fe-s cluster requiring proteins |
| NZ733115A (en) * | 2010-05-24 | 2019-02-22 | Xyleco Inc | Processing biomass |
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| WO2013043801A1 (en) * | 2011-09-20 | 2013-03-28 | Gevo, Inc. | High-performance dihydroxy acid dehydratases |
| JP2015501643A (ja) | 2011-12-09 | 2015-01-19 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | 発酵ブロスから生成物アルコールを取り出す方法 |
| CA2861613A1 (en) | 2011-12-30 | 2013-07-04 | Butamax Advanced Biofuels Llc | Fermentative production of alcohols |
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| US20140024064A1 (en) | 2012-07-23 | 2014-01-23 | Butamax(Tm) Advanced Biofuels Llc | Processes and systems for the production of fermentative alcohols |
| BR112015003701A2 (pt) | 2012-08-22 | 2017-12-12 | Butamax Advanced Biofuels Llc | células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição |
| BR112015005439A8 (pt) | 2012-09-12 | 2022-01-11 | Butamax Advanced Biofuels Llc | Método para recuperar um produto alcoólico de um caldo de fermentação e sistema |
| US9840724B2 (en) | 2012-09-21 | 2017-12-12 | Butamax Advanced Biofuels Llc | Production of renewable hydrocarbon compositions |
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| US20140093931A1 (en) | 2012-09-28 | 2014-04-03 | Butamax Advanced Biofuels Llc | Production of fermentation products |
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| BR112015008077A2 (pt) | 2012-10-11 | 2017-12-05 | Butamax Advanced Biofuels Llc | métodos para a produção de um produto de fermentação |
| US9650624B2 (en) | 2012-12-28 | 2017-05-16 | Butamax Advanced Biofuels Llc | DHAD variants for butanol production |
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| US9523104B2 (en) | 2013-03-12 | 2016-12-20 | Butamax Advanced Biofuels Llc | Processes and systems for the production of alcohols |
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| US9580705B2 (en) | 2013-03-15 | 2017-02-28 | Butamax Advanced Biofuels Llc | DHAD variants and methods of screening |
| WO2014151645A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Process for maximizing biomass growth and butanol yield by feedback control |
| WO2014144210A2 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
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| US9156760B2 (en) | 2013-03-15 | 2015-10-13 | Butamax Advanced Biofuels Llc | Method for production of butanol using extractive fermentation |
| WO2015002913A1 (en) | 2013-07-03 | 2015-01-08 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| EP3180440A4 (en) | 2014-08-11 | 2018-01-10 | Butamax Advanced Biofuels LLC | Yeast preparations and methods of making the same |
| WO2018157942A1 (en) | 2017-03-03 | 2018-09-07 | Nmr - Bio | Cell-free synthesis of isotopic labelled proteins from amino-acids precursors |
| CN111893083A (zh) * | 2019-05-05 | 2020-11-06 | 中国科学院上海高等研究院 | 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用 |
| DE102022119024A1 (de) * | 2022-07-28 | 2024-02-08 | INM - Leibniz-Institut für Neue Materialien gemeinnützige Gesellschaft mit beschränkter Haftung | Neuartige genetische Werkzeuge |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| FR2696190B1 (fr) * | 1992-09-25 | 1994-12-09 | Agronomique Inst Nat Rech | Acide nucléique codant pour une alpha-acétolactate synthase et ses applications. |
| US6800744B1 (en) * | 1997-07-02 | 2004-10-05 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics |
| DE19855312A1 (de) | 1998-12-01 | 2000-06-08 | Degussa | Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien |
| EP1887081A2 (en) * | 1999-02-25 | 2008-02-13 | Ceres Incorporated | DNA Sequences |
| US7273769B1 (en) | 2000-08-16 | 2007-09-25 | Micron Technology, Inc. | Method and apparatus for removing encapsulating material from a packaged microelectronic device |
| BR0115558A (pt) * | 2000-11-22 | 2004-08-17 | Cargill Dow Polymers Llc | ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático |
| US20110081348A1 (en) | 2004-12-01 | 2011-04-07 | Nina Brogden | Fungal signalling and metabolic enzymes |
| CA2603774C (en) * | 2005-04-12 | 2015-11-17 | E.I. Du Pont De Nemours And Company | System and process for biomass treatment |
| CA2617734A1 (en) * | 2005-08-12 | 2007-02-22 | Suntory Limited | Dihydroxy-acid dehydratase gene and use thereof |
| MX359740B (es) * | 2005-10-26 | 2018-10-09 | Du Pont | Produccion fermentativa de alcoholes de cuatro atomos de carbono. |
| WO2007106524A2 (en) | 2006-03-13 | 2007-09-20 | Cargill Inc. | Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol |
| US8206970B2 (en) * | 2006-05-02 | 2012-06-26 | Butamax(Tm) Advanced Biofuels Llc | Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase |
| PL389665A1 (pl) | 2007-02-09 | 2010-07-05 | The Regents of the University of CaliforniaThe Regents of the University of California | Wytwarzanie biopaliwa przez rekombinowane mikroorganizmy |
| EP2134849B1 (en) * | 2007-04-18 | 2013-07-10 | Butamax (TM) Advanced Biofuels LLC | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| WO2009086423A2 (en) | 2007-12-23 | 2009-07-09 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| DE102008010121B4 (de) | 2008-02-20 | 2013-11-21 | Butalco Gmbh | Fermentative Produktion von Isobutanol mit Hefe |
| AU2009256148B2 (en) * | 2008-06-04 | 2014-11-27 | Butamax(Tm) Advanced Biofuels Llc | A method for producing butanol using two-phase extractive fermentation |
| BRPI0909989A2 (pt) * | 2008-06-05 | 2021-06-22 | Butamax Advanced Biofuels Llc | célula de levedura recombinante e método para a produção de um produto |
| CA2736420A1 (en) | 2008-09-29 | 2010-04-01 | Butamaxtm Advanced Biofuels Llc | Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria |
| MX2011003238A (es) | 2008-09-29 | 2011-04-28 | Butamax Tm Advanced Biofuels | Identificacion y uso de dihidroxiacido deshidratasas [2fe-2s] bacterianas. |
| US9206447B2 (en) * | 2008-09-29 | 2015-12-08 | Butamax Advanced Biofuels Llc | Recombinant yeast host cells comprising FE-S cluster proteins |
| WO2010037119A1 (en) | 2008-09-29 | 2010-04-01 | Butamax™ Advanced Biofuels LLC | Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria |
| AU2009296227A1 (en) | 2008-09-29 | 2010-04-01 | Butamax(Tm) Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
| US8828694B2 (en) * | 2008-11-13 | 2014-09-09 | Butamax Advanced Biofuels Llc | Production of isobutanol in yeast mitochondria |
| BR112012003241A2 (pt) * | 2009-08-12 | 2015-09-01 | Gevo Inc | Localização de via de isobutanol citosólico para a produção de isobutanol |
| AU2010300642B2 (en) * | 2009-09-29 | 2015-05-14 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes |
| EP2483401B1 (en) * | 2009-09-29 | 2017-06-21 | Butamax (TM) Advanced Biofuels LLC | Improved yeast production host cells |
| CA2779884A1 (en) * | 2009-11-23 | 2011-05-26 | Butamax(Tm) Advanced Biofuels Llc | Method for producing butanol using extractive fermentation with electrolyte addition |
| BR112012012211A2 (pt) * | 2009-11-23 | 2017-05-09 | Butamax (Tm) Advanced Biofuels Llc | método para a recuperação de butanol e método para a produção de butanol |
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2009
- 2009-09-29 MX MX2011003238A patent/MX2011003238A/es not_active Application Discontinuation
- 2009-09-29 CN CN200980138542.2A patent/CN102186973B/zh not_active Expired - Fee Related
- 2009-09-29 JP JP2011529364A patent/JP5846912B2/ja active Active
- 2009-09-29 KR KR1020117009692A patent/KR101659101B1/ko not_active Expired - Fee Related
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- 2009-09-29 US US12/569,636 patent/US20100081154A1/en not_active Abandoned
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- 2009-09-29 WO PCT/US2009/058827 patent/WO2010037112A1/en not_active Ceased
- 2009-09-29 CA CA2735690A patent/CA2735690A1/en not_active Abandoned
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- 2013-12-20 ZA ZA2013/09658A patent/ZA201309658B/en unknown
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| ZA201309658B (en) | 2016-06-29 |
| CN102186973B (zh) | 2017-08-15 |
| NZ591244A (en) | 2013-03-28 |
| ES2521675T3 (es) | 2014-11-13 |
| US20140030776A1 (en) | 2014-01-30 |
| BRPI0913682A2 (pt) | 2020-08-11 |
| US20140051137A1 (en) | 2014-02-20 |
| KR20110063575A (ko) | 2011-06-10 |
| EP2821484A1 (en) | 2015-01-07 |
| ZA201101367B (en) | 2014-03-26 |
| EP2337851A1 (en) | 2011-06-29 |
| EP2821484B1 (en) | 2016-06-29 |
| MX2011003238A (es) | 2011-04-28 |
| WO2010037112A1 (en) | 2010-04-01 |
| US20100081154A1 (en) | 2010-04-01 |
| AU2009296225B2 (en) | 2015-09-17 |
| CN102186973A (zh) | 2011-09-14 |
| JP5846912B2 (ja) | 2016-01-20 |
| EP2337851B1 (en) | 2014-08-13 |
| AU2009296225A1 (en) | 2010-04-01 |
| KR101659101B1 (ko) | 2016-09-22 |
| JP2012503993A (ja) | 2012-02-16 |
| US8951937B2 (en) | 2015-02-10 |
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