CA2729160C - A chimeric bacteriophage lysin with activity against staphylococci bacteria - Google Patents

A chimeric bacteriophage lysin with activity against staphylococci bacteria Download PDF

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Publication number
CA2729160C
CA2729160C CA2729160A CA2729160A CA2729160C CA 2729160 C CA2729160 C CA 2729160C CA 2729160 A CA2729160 A CA 2729160A CA 2729160 A CA2729160 A CA 2729160A CA 2729160 C CA2729160 C CA 2729160C
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CA
Canada
Prior art keywords
lysin
chimeric
bacteriophage lysin
chimeric bacteriophage
clys
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2729160A
Other languages
English (en)
French (fr)
Other versions
CA2729160A1 (en
Inventor
Vincent A. Fischetti
Anu Daniel
Chad Euler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Rockefeller University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rockefeller University filed Critical Rockefeller University
Priority to CA2984933A priority Critical patent/CA2984933C/en
Publication of CA2729160A1 publication Critical patent/CA2729160A1/en
Application granted granted Critical
Publication of CA2729160C publication Critical patent/CA2729160C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2729160A 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria Active CA2729160C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2984933A CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7827708P 2008-07-03 2008-07-03
US61/078,277 2008-07-03
PCT/US2009/049349 WO2010002959A2 (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2984933A Division CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Publications (2)

Publication Number Publication Date
CA2729160A1 CA2729160A1 (en) 2010-01-07
CA2729160C true CA2729160C (en) 2017-11-14

Family

ID=41466590

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2729160A Active CA2729160C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria
CA2984933A Active CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2984933A Active CA2984933C (en) 2008-07-03 2009-07-01 A chimeric bacteriophage lysin with activity against staphylococci bacteria

Country Status (8)

Country Link
US (4) US8840900B2 (enExample)
EP (2) EP2307557B1 (enExample)
JP (2) JP5758291B2 (enExample)
AU (2) AU2009266982B2 (enExample)
CA (2) CA2729160C (enExample)
ES (1) ES2627321T3 (enExample)
HK (1) HK1248722A1 (enExample)
WO (1) WO2010002959A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008018854A2 (en) 2005-06-06 2008-02-14 The Rockefeller University Bactiophage lysins for bacillus anthracis
ES2357626T3 (es) 2005-08-24 2011-04-28 The Rockefeller University Lisinas mutantes de ply-gbs.
US9034322B2 (en) 2011-04-21 2015-05-19 The Rockefeller University Streptococcus bacteriophage lysins for detection and treatment of gram positive bacteria
DK2705144T4 (da) 2011-05-04 2019-10-21 Micreos Human Health Bv Polypeptid
WO2013052643A1 (en) 2011-10-05 2013-04-11 The Rockefeller University Dimeric bacteriophage lysins
ES2729123T3 (es) * 2012-05-09 2019-10-30 Contrafect Corp Rotura de biopelícula con lisina plyss2
MX389127B (es) * 2012-05-09 2025-03-19 Contrafect Corp Lisina de bacteriófago y combinaciones de antibióticos contra bacterias gram positivas.
CN103122347B (zh) * 2013-02-01 2015-04-22 中国科学院武汉病毒研究所 一种杀灭葡萄球菌的裂解酶及其应用
ES2525954B1 (es) * 2013-05-28 2015-10-23 Consejo Superior De Investigaciones Científicas (Csic) Enzibióticos bactericidas mejorados frente a neumococo y otras bacterias
CN104762285B (zh) * 2015-04-23 2017-10-27 武汉菲吉乐科生物科技有限公司 一种自内裂解大肠杆菌的裂解酶及其应用
CN104805066B (zh) * 2015-05-18 2018-04-24 武汉菲吉乐科生物科技有限公司 一种葡萄球菌裂解酶及其应用
RU2754667C2 (ru) 2016-05-12 2021-09-06 Контрафект Корпорейшн Способ микроразбавления в жидкой среде для оценки и определения минимальной ингибирующей концентрации антибактериальных полипептидов
JP2021527699A (ja) * 2018-06-22 2021-10-14 コントラフェクト コーポレイション 溶解素及びその誘導体がスタフィロコッカス・アウレウス及びグラム陽性細菌を抗生物質に再感作する
CN113038960A (zh) * 2018-08-10 2021-06-25 明治制果药业株式会社 噬菌体剂
CN109666667A (zh) * 2019-01-22 2019-04-23 上海交通大学 杀灭金黄色葡萄球菌的嵌合酶抗生素及其制备和应用
WO2021084558A2 (en) * 2019-10-31 2021-05-06 Bactoclear Holdings Pte. Ltd Chimeric proteins for selective lysis of bacteria
EP4114422A4 (en) * 2020-03-06 2024-09-18 Endolytix Technology, Inc. Compositions and methods for the treatment of intracellular bacterial infections
CN115605587B (zh) * 2020-03-11 2026-02-13 特卢姆治疗有限公司 新型重组溶素及其在治疗革兰氏阴性菌感染中的用途
CN111808837A (zh) * 2020-07-17 2020-10-23 青岛诺安百特生物技术有限公司 一种金黄色葡萄球菌噬菌体裂解酶及其制备方法和应用
CN115820616A (zh) * 2022-07-22 2023-03-21 昆明理工大学 一种带荧光标记的噬菌体裂解酶及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997008553A1 (en) * 1995-08-22 1997-03-06 The Regents Of The University Of California Targeting of proteins to the cell wall of gram-positive bacteria
US6056954A (en) * 1997-10-31 2000-05-02 New Horizons Diagnostics Corp Use of bacterial phage associated lysing enzymers for the prophylactic and therapeutic treatment of various illnesses
US6703025B1 (en) * 1998-08-31 2004-03-09 Inhibitex, Inc. Multicomponent vaccines
US20020127215A1 (en) * 1999-09-14 2002-09-12 Lawrence Loomis Parenteral use of bacterial phage associated lysing enzymes for the therapeutic treatment of bacterial infections
GB0107661D0 (en) * 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
US20040213765A1 (en) * 2001-07-13 2004-10-28 Vincent Fischetti Use of bacterial phage associated lytic enzymes to prevent food poisoning
CN1643139A (zh) * 2002-03-25 2005-07-20 沃里克大学 抗菌剂
AU2003261782A1 (en) * 2002-08-28 2004-03-19 Techno Network Shikoku Co., Ltd. Bacteriophages and remedies for bacterial infection
ES2357626T3 (es) * 2005-08-24 2011-04-28 The Rockefeller University Lisinas mutantes de ply-gbs.
WO2009024327A2 (en) 2007-08-22 2009-02-26 Profos Ag New proteins for use in human and animal staphylococcus infections
JP5732397B2 (ja) * 2008-10-10 2015-06-10 ルソメディカメンタ, エス.エー.Lusomedicamenta, S.A. 抗菌性ファージペプチド及びその使用法
EP3115054B1 (en) * 2009-02-06 2019-11-20 Technophage, Investigação E Desenvolvimento Em Biotecnologia, SA Antibacterial phage, phage peptides and methods of use thereof

Also Published As

Publication number Publication date
JP5758291B2 (ja) 2015-08-05
AU2009266982B2 (en) 2015-06-25
JP2015126738A (ja) 2015-07-09
JP2011526920A (ja) 2011-10-20
JP6169632B2 (ja) 2017-07-26
US20150064156A1 (en) 2015-03-05
US11827911B2 (en) 2023-11-28
US20190055533A1 (en) 2019-02-21
US20120258088A1 (en) 2012-10-11
AU2015230807B2 (en) 2017-07-27
EP2307557A2 (en) 2011-04-13
CA2729160A1 (en) 2010-01-07
US8840900B2 (en) 2014-09-23
AU2009266982A1 (en) 2010-01-07
EP2307557A4 (en) 2012-04-11
CA2984933A1 (en) 2010-01-07
US10053681B2 (en) 2018-08-21
US11015182B2 (en) 2021-05-25
EP2307557B1 (en) 2017-03-01
AU2015230807A1 (en) 2015-10-15
WO2010002959A3 (en) 2010-05-06
ES2627321T3 (es) 2017-07-27
CA2984933C (en) 2020-07-07
US20210348146A1 (en) 2021-11-11
EP3263588A1 (en) 2018-01-03
HK1248722A1 (en) 2018-10-19
WO2010002959A2 (en) 2010-01-07

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